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Interactions between hypoxia tolerance and food deprivation in Amazonian oscars, Astronotus ocellatus

Detalhes bibliográficos
Autor(a) principal: Boeck, Gudrun de
Data de Publicação: 2013
Outros Autores: Wood, Chris M., Iftikar, Fathima I., Matey, Victoria E., Scott, Graham R., Sloman, Katherine A., Nazar Paula da Silva, Maria de, Almeida-Val, Vera Maria Fonseca, Val, Adalberto Luis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INPA
Texto Completo: https://repositorio.inpa.gov.br/handle/1/15056
Resumo: Oscars are often subjected to a combination of low levels of oxygen and fasting during nest-guarding on Amazonian floodplains. We questioned whether this anorexia would aggravate the osmo-respiratory compromise. We compared fed and fasted oscars (1014 days) in both normoxia and hypoxia (1020 Torr, 4 h). Routine oxygen consumption rates (MO2) were increased by 75% in fasted fish, reflecting behavioural differences, whereas fasting improved hypoxia resistance and critical oxygen tensions (Pcrit) lowered from 54 Torr in fed fish to 34 Torr when fasting. In fed fish, hypoxia reduced liver lipid stores by approximately 50% and total liver energy content by 30%. Fasted fish had a 50% lower hepatosomatic index, resulting in lower total liver protein, glycogen and lipid energy stores under normoxia. Compared with hypoxic fed fish, hypoxic fasted fish only showed reduced liver protein levels and even gained glycogen (+50%) on a per gram basis. This confirms the hypothesis that hypoxia-tolerant fish protect their glycogen stores as much as possible as a safeguard for more prolonged hypoxic events. In general, fasted fish showed lower hydroxyacylCoA dehydrogenase activities compared with fed fish, although this effect was only significant in hypoxic fasted fish. Energy stores and activities of enzymes related to energy metabolism in muscle or gills were not affected. Branchial Na+ uptake rates were more than two times lower in fed fish, whereas Na+ efflux was similar. Fed and fasted fish quickly reduced Na+ uptake and efflux during hypoxia, with fasting fish responding more rapidly. Ammonia excretion and K+ efflux were reduced under hypoxia, indicating decreased transcellular permeability. Fasted fish had more mitochondria-rich cells (MRC), with larger crypts, indicating the increased importance of the branchial uptake route when feeding is limited. Gill MRC density and surface area were greatly reduced under hypoxia, possibly to reduce ion uptake and efflux rates. Density of mucous cells of normoxic fasted fish was approximately fourfold of that in fed fish. Overall, a 1014 day fasting period had no negative effects on hypoxia tolerance in oscars, as fasted fish were able to respond more quickly to lower oxygen levels, and reduced branchial permeability effectively. © 2013. Published by The Company of Biologists Ltd.
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spelling Boeck, Gudrun deWood, Chris M.Iftikar, Fathima I.Matey, Victoria E.Scott, Graham R.Sloman, Katherine A.Nazar Paula da Silva, Maria deAlmeida-Val, Vera Maria FonsecaVal, Adalberto Luis2020-05-07T14:02:15Z2020-05-07T14:02:15Z2013https://repositorio.inpa.gov.br/handle/1/1505610.1242/jeb.082891Oscars are often subjected to a combination of low levels of oxygen and fasting during nest-guarding on Amazonian floodplains. We questioned whether this anorexia would aggravate the osmo-respiratory compromise. We compared fed and fasted oscars (1014 days) in both normoxia and hypoxia (1020 Torr, 4 h). Routine oxygen consumption rates (MO2) were increased by 75% in fasted fish, reflecting behavioural differences, whereas fasting improved hypoxia resistance and critical oxygen tensions (Pcrit) lowered from 54 Torr in fed fish to 34 Torr when fasting. In fed fish, hypoxia reduced liver lipid stores by approximately 50% and total liver energy content by 30%. Fasted fish had a 50% lower hepatosomatic index, resulting in lower total liver protein, glycogen and lipid energy stores under normoxia. Compared with hypoxic fed fish, hypoxic fasted fish only showed reduced liver protein levels and even gained glycogen (+50%) on a per gram basis. This confirms the hypothesis that hypoxia-tolerant fish protect their glycogen stores as much as possible as a safeguard for more prolonged hypoxic events. In general, fasted fish showed lower hydroxyacylCoA dehydrogenase activities compared with fed fish, although this effect was only significant in hypoxic fasted fish. Energy stores and activities of enzymes related to energy metabolism in muscle or gills were not affected. Branchial Na+ uptake rates were more than two times lower in fed fish, whereas Na+ efflux was similar. Fed and fasted fish quickly reduced Na+ uptake and efflux during hypoxia, with fasting fish responding more rapidly. Ammonia excretion and K+ efflux were reduced under hypoxia, indicating decreased transcellular permeability. Fasted fish had more mitochondria-rich cells (MRC), with larger crypts, indicating the increased importance of the branchial uptake route when feeding is limited. Gill MRC density and surface area were greatly reduced under hypoxia, possibly to reduce ion uptake and efflux rates. Density of mucous cells of normoxic fasted fish was approximately fourfold of that in fed fish. Overall, a 1014 day fasting period had no negative effects on hypoxia tolerance in oscars, as fasted fish were able to respond more quickly to lower oxygen levels, and reduced branchial permeability effectively. © 2013. Published by The Company of Biologists Ltd.Volume 216, Número 24, Pags. 4590-4600Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessOxygenAnimalsAnoxiaBreathingCichlidCritical Oxygen TensionEnergy MetabolismFood DeprivationGillHistologyIon CurrentIon TransportIonoregulationMetabolismOsmoregulationOxygen ConsumptionPhysiologyCritical Oxygen TensionEnergy MetabolismIon FluxIonoregulationRespirationAnimalAnoxiaCichlidsEnergy MetabolismFood DeprivationGillsIon TransportOsmoregulationOxygenOxygen ConsumptionRespirationInteractions between hypoxia tolerance and food deprivation in Amazonian oscars, Astronotus ocellatusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of Experimental Biologyengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf1999589https://repositorio.inpa.gov.br/bitstream/1/15056/1/artigo-inpa.pdfe1d12db19daf09ab350e6e6ec9b3f82fMD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/15056/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/150562020-07-14 10:43:25.618oai:repositorio:1/15056Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T14:43:25Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Interactions between hypoxia tolerance and food deprivation in Amazonian oscars, Astronotus ocellatus
title Interactions between hypoxia tolerance and food deprivation in Amazonian oscars, Astronotus ocellatus
spellingShingle Interactions between hypoxia tolerance and food deprivation in Amazonian oscars, Astronotus ocellatus
Boeck, Gudrun de
Oxygen
Animals
Anoxia
Breathing
Cichlid
Critical Oxygen Tension
Energy Metabolism
Food Deprivation
Gill
Histology
Ion Current
Ion Transport
Ionoregulation
Metabolism
Osmoregulation
Oxygen Consumption
Physiology
Critical Oxygen Tension
Energy Metabolism
Ion Flux
Ionoregulation
Respiration
Animal
Anoxia
Cichlids
Energy Metabolism
Food Deprivation
Gills
Ion Transport
Osmoregulation
Oxygen
Oxygen Consumption
Respiration
title_short Interactions between hypoxia tolerance and food deprivation in Amazonian oscars, Astronotus ocellatus
title_full Interactions between hypoxia tolerance and food deprivation in Amazonian oscars, Astronotus ocellatus
title_fullStr Interactions between hypoxia tolerance and food deprivation in Amazonian oscars, Astronotus ocellatus
title_full_unstemmed Interactions between hypoxia tolerance and food deprivation in Amazonian oscars, Astronotus ocellatus
title_sort Interactions between hypoxia tolerance and food deprivation in Amazonian oscars, Astronotus ocellatus
author Boeck, Gudrun de
author_facet Boeck, Gudrun de
Wood, Chris M.
Iftikar, Fathima I.
Matey, Victoria E.
Scott, Graham R.
Sloman, Katherine A.
Nazar Paula da Silva, Maria de
Almeida-Val, Vera Maria Fonseca
Val, Adalberto Luis
author_role author
author2 Wood, Chris M.
Iftikar, Fathima I.
Matey, Victoria E.
Scott, Graham R.
Sloman, Katherine A.
Nazar Paula da Silva, Maria de
Almeida-Val, Vera Maria Fonseca
Val, Adalberto Luis
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Boeck, Gudrun de
Wood, Chris M.
Iftikar, Fathima I.
Matey, Victoria E.
Scott, Graham R.
Sloman, Katherine A.
Nazar Paula da Silva, Maria de
Almeida-Val, Vera Maria Fonseca
Val, Adalberto Luis
dc.subject.eng.fl_str_mv Oxygen
Animals
Anoxia
Breathing
Cichlid
Critical Oxygen Tension
Energy Metabolism
Food Deprivation
Gill
Histology
Ion Current
Ion Transport
Ionoregulation
Metabolism
Osmoregulation
Oxygen Consumption
Physiology
Critical Oxygen Tension
Energy Metabolism
Ion Flux
Ionoregulation
Respiration
Animal
Anoxia
Cichlids
Energy Metabolism
Food Deprivation
Gills
Ion Transport
Osmoregulation
Oxygen
Oxygen Consumption
Respiration
topic Oxygen
Animals
Anoxia
Breathing
Cichlid
Critical Oxygen Tension
Energy Metabolism
Food Deprivation
Gill
Histology
Ion Current
Ion Transport
Ionoregulation
Metabolism
Osmoregulation
Oxygen Consumption
Physiology
Critical Oxygen Tension
Energy Metabolism
Ion Flux
Ionoregulation
Respiration
Animal
Anoxia
Cichlids
Energy Metabolism
Food Deprivation
Gills
Ion Transport
Osmoregulation
Oxygen
Oxygen Consumption
Respiration
description Oscars are often subjected to a combination of low levels of oxygen and fasting during nest-guarding on Amazonian floodplains. We questioned whether this anorexia would aggravate the osmo-respiratory compromise. We compared fed and fasted oscars (1014 days) in both normoxia and hypoxia (1020 Torr, 4 h). Routine oxygen consumption rates (MO2) were increased by 75% in fasted fish, reflecting behavioural differences, whereas fasting improved hypoxia resistance and critical oxygen tensions (Pcrit) lowered from 54 Torr in fed fish to 34 Torr when fasting. In fed fish, hypoxia reduced liver lipid stores by approximately 50% and total liver energy content by 30%. Fasted fish had a 50% lower hepatosomatic index, resulting in lower total liver protein, glycogen and lipid energy stores under normoxia. Compared with hypoxic fed fish, hypoxic fasted fish only showed reduced liver protein levels and even gained glycogen (+50%) on a per gram basis. This confirms the hypothesis that hypoxia-tolerant fish protect their glycogen stores as much as possible as a safeguard for more prolonged hypoxic events. In general, fasted fish showed lower hydroxyacylCoA dehydrogenase activities compared with fed fish, although this effect was only significant in hypoxic fasted fish. Energy stores and activities of enzymes related to energy metabolism in muscle or gills were not affected. Branchial Na+ uptake rates were more than two times lower in fed fish, whereas Na+ efflux was similar. Fed and fasted fish quickly reduced Na+ uptake and efflux during hypoxia, with fasting fish responding more rapidly. Ammonia excretion and K+ efflux were reduced under hypoxia, indicating decreased transcellular permeability. Fasted fish had more mitochondria-rich cells (MRC), with larger crypts, indicating the increased importance of the branchial uptake route when feeding is limited. Gill MRC density and surface area were greatly reduced under hypoxia, possibly to reduce ion uptake and efflux rates. Density of mucous cells of normoxic fasted fish was approximately fourfold of that in fed fish. Overall, a 1014 day fasting period had no negative effects on hypoxia tolerance in oscars, as fasted fish were able to respond more quickly to lower oxygen levels, and reduced branchial permeability effectively. © 2013. Published by The Company of Biologists Ltd.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2020-05-07T14:02:15Z
dc.date.available.fl_str_mv 2020-05-07T14:02:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/15056
dc.identifier.doi.none.fl_str_mv 10.1242/jeb.082891
url https://repositorio.inpa.gov.br/handle/1/15056
identifier_str_mv 10.1242/jeb.082891
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Volume 216, Número 24, Pags. 4590-4600
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Journal of Experimental Biology
publisher.none.fl_str_mv Journal of Experimental Biology
dc.source.none.fl_str_mv reponame:Repositório Institucional do INPA
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Repositório Institucional do INPA
collection Repositório Institucional do INPA
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