Fine specificities of two lectins from Cymbosema roseum seeds: A lectin specific for high-mannose oligosaccharides and a lectin specific for blood group H type II trisaccharide

• Autor(a) principal: Dam, Tarun Kanti
• Data de Publicação: 2011
• Outros Autores: Cavada, B. S., Nagano, C. S., Rocha, Bruno Anderson Matias da, Benevides, R. G., Nascimento, K. S., Sousa, Luiz A.G. de, Oscarson, Stefan, Brewer, Curtis Fred
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional do INPA
• Texto Completo: https://repositorio.inpa.gov.br/handle/1/16195
• topic: Blood Group H Antigen; Epitope; Fucose; Lectin; Mannose Oligosaccharide; Trisaccharide; Chemical Structure; Cymbosema Roseum; Hemagglutination; Legume; Nonhuman; Seed Plant; Priority Journal; Protein Binding; Protein Purification; Thermodynamics; Abo Blood-group System; Animal; Chromatography, Affinity; Erythrocytes; Fabaceae; Fucose; Hemagglutination Inhibition Tests; Mannose; Mannose-binding Lectins; Oligosaccharides; Plant Lectins; Polysaccharides; Rabbits; Seeds; Thermodynamics; Cymbosema Roseum

The legume species of Cymbosema roseum of Diocleinae subtribe produce at least two different seed lectins. The present study demonstrates that C. roseum lectin I (CRL I) binds with high affinity to the "core" trimannoside of N-linked oligosaccharides. Cymbosema roseum lectin II (CRL II), on the other hand, binds with high affinity to the blood group H trisaccharide (Fuc1,2Gal1-4GlcNAc-). Thermodynamic and hemagglutination inhibition studies reveal the fine binding specificities of the two lectins. Data obtained with a complete set of monodeoxy analogs of the core trimannoside indicate that CRL I recognizes the 3-, 4- and 6-hydroxyl groups of the (1,6) Man residue, the 3- and 4-hydroxyl group of the (1,3) Man residue and the 2- and 4-hydroxyl groups of the central Man residue of the trimannoside. CRL I possesses enhanced affinities for the Man5 oligomannose glycan and a biantennary complex glycan as well as glycoproteins containing high-mannose glycans. On the other hand, CRL II distinguishes the blood group H type II epitope from the Lewisx, Lewisy, Lewisa and Lewisb epitopes. CRL II also distinguishes between blood group H type II and type I trisaccharides. CRL I and CRL II, respectively, possess differences in fine specificities when compared with other reported mannose and fucose recognizing lectins. This is the first report of a mannose-specific lectin (CRL I) and a blood group H type II-specific lectin (CRL II) from seeds of a member of the Diocleinae subtribe. © 2011 The Author.