Biology of amazonian mosquitoes. III. Esterase isozymes in Anopheles darlingi.

Detalhes bibliográficos
Autor(a) principal: Santos,Joselita M.M. dos
Data de Publicação: 1985
Outros Autores: Contel,Eucleia P.B., Kerr,Warwick E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Amazonica
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59671985000100167
Resumo: Six esterase isozymes were studied during the development of Anopheles darlingi by using polyacrylamide gel electrophoresis and two different substrates, a-naphthylcelate and a-naphthylpropionate. Esterases 5 and 6'were detected in all developmental stages esterases 1 and 2 were more intensively stained if larvae, while esterases 3 and 4 were better visualized in pupae and adults. Strong differences in intensity of some of the isozymes were observed during the pupal stage.Four out of the six isozymes showed variation in the electrophoretic mobility. Esterase-2 was choosed for genetic studies, because was the best stained isozyme in the gels. Two codominant alleles {Est2*S and Est2*F) code for this polymorphic system, with the Est*S frequency equal to 0.521. Phenotypic distribution is in agreement with hardy-Weinberg expectations.
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spelling Biology of amazonian mosquitoes. III. Esterase isozymes in Anopheles darlingi.Six esterase isozymes were studied during the development of Anopheles darlingi by using polyacrylamide gel electrophoresis and two different substrates, a-naphthylcelate and a-naphthylpropionate. Esterases 5 and 6'were detected in all developmental stages esterases 1 and 2 were more intensively stained if larvae, while esterases 3 and 4 were better visualized in pupae and adults. Strong differences in intensity of some of the isozymes were observed during the pupal stage.Four out of the six isozymes showed variation in the electrophoretic mobility. Esterase-2 was choosed for genetic studies, because was the best stained isozyme in the gels. Two codominant alleles {Est2*S and Est2*F) code for this polymorphic system, with the Est*S frequency equal to 0.521. Phenotypic distribution is in agreement with hardy-Weinberg expectations.Instituto Nacional de Pesquisas da Amazônia1985-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59671985000100167Acta Amazonica v.15 n.1-2 1985reponame:Acta Amazonicainstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPA10.1590/1809-43921985152177info:eu-repo/semantics/openAccessSantos,Joselita M.M. dosContel,Eucleia P.B.Kerr,Warwick E.eng2015-09-29T00:00:00Zoai:scielo:S0044-59671985000100167Revistahttps://acta.inpa.gov.br/PUBhttps://old.scielo.br/oai/scielo-oai.phpacta@inpa.gov.br||acta@inpa.gov.br1809-43920044-5967opendoar:2015-09-29T00:00Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.none.fl_str_mv Biology of amazonian mosquitoes. III. Esterase isozymes in Anopheles darlingi.
title Biology of amazonian mosquitoes. III. Esterase isozymes in Anopheles darlingi.
spellingShingle Biology of amazonian mosquitoes. III. Esterase isozymes in Anopheles darlingi.
Santos,Joselita M.M. dos
title_short Biology of amazonian mosquitoes. III. Esterase isozymes in Anopheles darlingi.
title_full Biology of amazonian mosquitoes. III. Esterase isozymes in Anopheles darlingi.
title_fullStr Biology of amazonian mosquitoes. III. Esterase isozymes in Anopheles darlingi.
title_full_unstemmed Biology of amazonian mosquitoes. III. Esterase isozymes in Anopheles darlingi.
title_sort Biology of amazonian mosquitoes. III. Esterase isozymes in Anopheles darlingi.
author Santos,Joselita M.M. dos
author_facet Santos,Joselita M.M. dos
Contel,Eucleia P.B.
Kerr,Warwick E.
author_role author
author2 Contel,Eucleia P.B.
Kerr,Warwick E.
author2_role author
author
dc.contributor.author.fl_str_mv Santos,Joselita M.M. dos
Contel,Eucleia P.B.
Kerr,Warwick E.
description Six esterase isozymes were studied during the development of Anopheles darlingi by using polyacrylamide gel electrophoresis and two different substrates, a-naphthylcelate and a-naphthylpropionate. Esterases 5 and 6'were detected in all developmental stages esterases 1 and 2 were more intensively stained if larvae, while esterases 3 and 4 were better visualized in pupae and adults. Strong differences in intensity of some of the isozymes were observed during the pupal stage.Four out of the six isozymes showed variation in the electrophoretic mobility. Esterase-2 was choosed for genetic studies, because was the best stained isozyme in the gels. Two codominant alleles {Est2*S and Est2*F) code for this polymorphic system, with the Est*S frequency equal to 0.521. Phenotypic distribution is in agreement with hardy-Weinberg expectations.
publishDate 1985
dc.date.none.fl_str_mv 1985-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59671985000100167
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59671985000100167
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1809-43921985152177
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Nacional de Pesquisas da Amazônia
publisher.none.fl_str_mv Instituto Nacional de Pesquisas da Amazônia
dc.source.none.fl_str_mv Acta Amazonica v.15 n.1-2 1985
reponame:Acta Amazonica
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Acta Amazonica
collection Acta Amazonica
repository.name.fl_str_mv Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA)
repository.mail.fl_str_mv acta@inpa.gov.br||acta@inpa.gov.br
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