Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulp

Detalhes bibliográficos
Autor(a) principal: CARDOSO,Gabrielle Virgínia Ferreira
Data de Publicação: 2021
Outros Autores: OLIVEIRA,Andrey Carlos do Sacramento de, SILVA,Andréia Silva da, SILVA,Marcos Clécio de Lemos, LIMA,Joelson Sousa, ROOS,Talita Bandeira, MORAES,Carina Martins de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Amazonica
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672021000100079
Resumo: ABSTRACT Chagas disease, caused by the protozoan Trypanosoma cruzi, has often been linked to oral transmission through açai consumption. Molecular methods that allow fast and accurate identification of the pathogen are important for the detection of the presence of the parasite in this food. This study aimed to optimize polymerase chain reaction (PCR)-based detection of T. cruzi DNA in açai pulp. Several dilutions of T. cruzi DTU TcI trypomastigote forms were cultured in liver infusion tryptose (LIT) medium. Trypanosoma cruzi DNA was extracted from the cells and subjected to PCR. Subsequently, culture dilutions were added to açai pulp to evaluate the detection threshold of the optimized PCR assay. We demonstrate that our assay can detect T. cruzi DNA in açai pulp at a concentration of 1.08 × 10-10 ng µL-1. We conclude that our optimized protocol is effective and can be used as an important tool for the detection of T. cruzi contamination in açaí.
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spelling Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulpdetection methodfoodborne diseasefood pathogenmolecular detectionChagas diseaseABSTRACT Chagas disease, caused by the protozoan Trypanosoma cruzi, has often been linked to oral transmission through açai consumption. Molecular methods that allow fast and accurate identification of the pathogen are important for the detection of the presence of the parasite in this food. This study aimed to optimize polymerase chain reaction (PCR)-based detection of T. cruzi DNA in açai pulp. Several dilutions of T. cruzi DTU TcI trypomastigote forms were cultured in liver infusion tryptose (LIT) medium. Trypanosoma cruzi DNA was extracted from the cells and subjected to PCR. Subsequently, culture dilutions were added to açai pulp to evaluate the detection threshold of the optimized PCR assay. We demonstrate that our assay can detect T. cruzi DNA in açai pulp at a concentration of 1.08 × 10-10 ng µL-1. We conclude that our optimized protocol is effective and can be used as an important tool for the detection of T. cruzi contamination in açaí.Instituto Nacional de Pesquisas da Amazônia2021-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672021000100079Acta Amazonica v.51 n.1 2021reponame:Acta Amazonicainstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPA10.1590/1809-4392201903426info:eu-repo/semantics/openAccessCARDOSO,Gabrielle Virgínia FerreiraOLIVEIRA,Andrey Carlos do Sacramento deSILVA,Andréia Silva daSILVA,Marcos Clécio de LemosLIMA,Joelson SousaROOS,Talita BandeiraMORAES,Carina Martins deeng2021-02-26T00:00:00Zoai:scielo:S0044-59672021000100079Revistahttps://acta.inpa.gov.br/PUBhttps://old.scielo.br/oai/scielo-oai.phpacta@inpa.gov.br||acta@inpa.gov.br1809-43920044-5967opendoar:2021-02-26T00:00Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.none.fl_str_mv Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulp
title Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulp
spellingShingle Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulp
CARDOSO,Gabrielle Virgínia Ferreira
detection method
foodborne disease
food pathogen
molecular detection
Chagas disease
title_short Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulp
title_full Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulp
title_fullStr Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulp
title_full_unstemmed Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulp
title_sort Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulp
author CARDOSO,Gabrielle Virgínia Ferreira
author_facet CARDOSO,Gabrielle Virgínia Ferreira
OLIVEIRA,Andrey Carlos do Sacramento de
SILVA,Andréia Silva da
SILVA,Marcos Clécio de Lemos
LIMA,Joelson Sousa
ROOS,Talita Bandeira
MORAES,Carina Martins de
author_role author
author2 OLIVEIRA,Andrey Carlos do Sacramento de
SILVA,Andréia Silva da
SILVA,Marcos Clécio de Lemos
LIMA,Joelson Sousa
ROOS,Talita Bandeira
MORAES,Carina Martins de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv CARDOSO,Gabrielle Virgínia Ferreira
OLIVEIRA,Andrey Carlos do Sacramento de
SILVA,Andréia Silva da
SILVA,Marcos Clécio de Lemos
LIMA,Joelson Sousa
ROOS,Talita Bandeira
MORAES,Carina Martins de
dc.subject.por.fl_str_mv detection method
foodborne disease
food pathogen
molecular detection
Chagas disease
topic detection method
foodborne disease
food pathogen
molecular detection
Chagas disease
description ABSTRACT Chagas disease, caused by the protozoan Trypanosoma cruzi, has often been linked to oral transmission through açai consumption. Molecular methods that allow fast and accurate identification of the pathogen are important for the detection of the presence of the parasite in this food. This study aimed to optimize polymerase chain reaction (PCR)-based detection of T. cruzi DNA in açai pulp. Several dilutions of T. cruzi DTU TcI trypomastigote forms were cultured in liver infusion tryptose (LIT) medium. Trypanosoma cruzi DNA was extracted from the cells and subjected to PCR. Subsequently, culture dilutions were added to açai pulp to evaluate the detection threshold of the optimized PCR assay. We demonstrate that our assay can detect T. cruzi DNA in açai pulp at a concentration of 1.08 × 10-10 ng µL-1. We conclude that our optimized protocol is effective and can be used as an important tool for the detection of T. cruzi contamination in açaí.
publishDate 2021
dc.date.none.fl_str_mv 2021-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672021000100079
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672021000100079
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1809-4392201903426
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Nacional de Pesquisas da Amazônia
publisher.none.fl_str_mv Instituto Nacional de Pesquisas da Amazônia
dc.source.none.fl_str_mv Acta Amazonica v.51 n.1 2021
reponame:Acta Amazonica
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Acta Amazonica
collection Acta Amazonica
repository.name.fl_str_mv Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA)
repository.mail.fl_str_mv acta@inpa.gov.br||acta@inpa.gov.br
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