Refractometry as an alternative to the biuret method for measuring total serum proteins in Podocnemis expansa (Podocnemididae) and Phrynops geoffroanus (Chelidae)

Detalhes bibliográficos
Autor(a) principal: PESSOA,Lourdes Marina Bezerra
Data de Publicação: 2017
Outros Autores: LIMA,Maíra Gonçalves da Mota, CARNEIRO,Filipe Tavares, ZANANI,Nathalia Salgado, SCALON,Marcela Corrêa, SILVA,Thamiris Figueiredo, LIMA,Mariana Accioly, ABRAHIM,Maia Araújo, PALUDO,Giane Regina
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Acta Amazonica
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000100083
Resumo: ABSTRACT Total serum protein is a significant indicator of health condition in animals. The aim of this study was to analyze the precision of the portable refractometer in determining the concentration of total serum proteins in Podocnemis expansa and Phrynops geoffroanus. A total of 26 animals were used. The blood samples were collected from the supraoccipital sinus and stored in tubes without anticoagulant. Total serum protein was determined using both the biuret reaction and refractometry. The total serum protein mean concentration (g dL-1) with biuret method and refractometry for P. expansa were 3.16 and 3.2; and for P. geoffroanus were 3.56 and 2.72, respectively. These results indicate that total serum protein values can be determined with precision in P. expansa and P. geoffroanus using a portable refractometer.
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spelling Refractometry as an alternative to the biuret method for measuring total serum proteins in Podocnemis expansa (Podocnemididae) and Phrynops geoffroanus (Chelidae)biuret methodrefractometryPodocnemis expansaPhrynops geoffroanusABSTRACT Total serum protein is a significant indicator of health condition in animals. The aim of this study was to analyze the precision of the portable refractometer in determining the concentration of total serum proteins in Podocnemis expansa and Phrynops geoffroanus. A total of 26 animals were used. The blood samples were collected from the supraoccipital sinus and stored in tubes without anticoagulant. Total serum protein was determined using both the biuret reaction and refractometry. The total serum protein mean concentration (g dL-1) with biuret method and refractometry for P. expansa were 3.16 and 3.2; and for P. geoffroanus were 3.56 and 2.72, respectively. These results indicate that total serum protein values can be determined with precision in P. expansa and P. geoffroanus using a portable refractometer.Instituto Nacional de Pesquisas da Amazônia2017-03-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000100083Acta Amazonica v.47 n.1 2017reponame:Acta Amazonicainstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPA10.1590/1809-4392201601383info:eu-repo/semantics/openAccessPESSOA,Lourdes Marina BezerraLIMA,Maíra Gonçalves da MotaCARNEIRO,Filipe TavaresZANANI,Nathalia SalgadoSCALON,Marcela CorrêaSILVA,Thamiris FigueiredoLIMA,Mariana AcciolyABRAHIM,Maia AraújoPALUDO,Giane Reginaeng2016-12-05T00:00:00Zoai:scielo:S0044-59672017000100083Revistahttps://acta.inpa.gov.br/PUBhttps://old.scielo.br/oai/scielo-oai.phpacta@inpa.gov.br||acta@inpa.gov.br1809-43920044-5967opendoar:2016-12-05T00:00Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.none.fl_str_mv Refractometry as an alternative to the biuret method for measuring total serum proteins in Podocnemis expansa (Podocnemididae) and Phrynops geoffroanus (Chelidae)
title Refractometry as an alternative to the biuret method for measuring total serum proteins in Podocnemis expansa (Podocnemididae) and Phrynops geoffroanus (Chelidae)
spellingShingle Refractometry as an alternative to the biuret method for measuring total serum proteins in Podocnemis expansa (Podocnemididae) and Phrynops geoffroanus (Chelidae)
PESSOA,Lourdes Marina Bezerra
biuret method
refractometry
Podocnemis expansa
Phrynops geoffroanus
title_short Refractometry as an alternative to the biuret method for measuring total serum proteins in Podocnemis expansa (Podocnemididae) and Phrynops geoffroanus (Chelidae)
title_full Refractometry as an alternative to the biuret method for measuring total serum proteins in Podocnemis expansa (Podocnemididae) and Phrynops geoffroanus (Chelidae)
title_fullStr Refractometry as an alternative to the biuret method for measuring total serum proteins in Podocnemis expansa (Podocnemididae) and Phrynops geoffroanus (Chelidae)
title_full_unstemmed Refractometry as an alternative to the biuret method for measuring total serum proteins in Podocnemis expansa (Podocnemididae) and Phrynops geoffroanus (Chelidae)
title_sort Refractometry as an alternative to the biuret method for measuring total serum proteins in Podocnemis expansa (Podocnemididae) and Phrynops geoffroanus (Chelidae)
author PESSOA,Lourdes Marina Bezerra
author_facet PESSOA,Lourdes Marina Bezerra
LIMA,Maíra Gonçalves da Mota
CARNEIRO,Filipe Tavares
ZANANI,Nathalia Salgado
SCALON,Marcela Corrêa
SILVA,Thamiris Figueiredo
LIMA,Mariana Accioly
ABRAHIM,Maia Araújo
PALUDO,Giane Regina
author_role author
author2 LIMA,Maíra Gonçalves da Mota
CARNEIRO,Filipe Tavares
ZANANI,Nathalia Salgado
SCALON,Marcela Corrêa
SILVA,Thamiris Figueiredo
LIMA,Mariana Accioly
ABRAHIM,Maia Araújo
PALUDO,Giane Regina
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv PESSOA,Lourdes Marina Bezerra
LIMA,Maíra Gonçalves da Mota
CARNEIRO,Filipe Tavares
ZANANI,Nathalia Salgado
SCALON,Marcela Corrêa
SILVA,Thamiris Figueiredo
LIMA,Mariana Accioly
ABRAHIM,Maia Araújo
PALUDO,Giane Regina
dc.subject.por.fl_str_mv biuret method
refractometry
Podocnemis expansa
Phrynops geoffroanus
topic biuret method
refractometry
Podocnemis expansa
Phrynops geoffroanus
description ABSTRACT Total serum protein is a significant indicator of health condition in animals. The aim of this study was to analyze the precision of the portable refractometer in determining the concentration of total serum proteins in Podocnemis expansa and Phrynops geoffroanus. A total of 26 animals were used. The blood samples were collected from the supraoccipital sinus and stored in tubes without anticoagulant. Total serum protein was determined using both the biuret reaction and refractometry. The total serum protein mean concentration (g dL-1) with biuret method and refractometry for P. expansa were 3.16 and 3.2; and for P. geoffroanus were 3.56 and 2.72, respectively. These results indicate that total serum protein values can be determined with precision in P. expansa and P. geoffroanus using a portable refractometer.
publishDate 2017
dc.date.none.fl_str_mv 2017-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000100083
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000100083
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1809-4392201601383
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Nacional de Pesquisas da Amazônia
publisher.none.fl_str_mv Instituto Nacional de Pesquisas da Amazônia
dc.source.none.fl_str_mv Acta Amazonica v.47 n.1 2017
reponame:Acta Amazonica
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Acta Amazonica
collection Acta Amazonica
repository.name.fl_str_mv Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA)
repository.mail.fl_str_mv acta@inpa.gov.br||acta@inpa.gov.br
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