Diversity and capacity to promote maize growth of bacteria isolated from the Amazon region
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Amazonica |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672016000200111 |
Resumo: | ABSTRACT Maize plants can establish beneficial associations with plant growth-promoting bacteria. However, few studies have been conducted on the characterization and inoculation of these bacteria in the Amazon region. This study aimed to characterize endophytic bacteria isolated from maize in the Amazon region and to assess their capacity to promote plant growth. Fifty-five bacterial isolates were obtained from maize grown in two types of ecosystems, i.e., a cerrado (savanna) and a forest area. The isolates were characterized by the presence of the nifH gene, their ability to synthesize indole-3-acetic acid (IAA) and solubilize calcium phosphate (CaHPO4), and 16S rRNA partial gene sequencing. Twenty-four bacteria contained the nifH gene, of which seven were isolated from maize plants cultivated in a cerrado area and seventeen from a forest area. Fourteen samples showed the capacity to synthesize IAA and only four solubilized calcium phosphate. The following genera were found among these isolates: Pseudomonas; Acinetobacter; Enterobacter; Pantoea; Burkholderia and Bacillus. In addition, eight isolates with plant growth-promoting capacity were selected for a glasshouse experiment involving the inoculation of two maize genotypes (a hybrid and a variety) grown in pots containing soil. Inoculation promoted the development of the maize plants but no significant interaction between maize cultivar and bacterial inoculation was found. A high diversity of endophytic bacteria is present in the Amazon region and these bacteria have potential to promote the development of maize plants. |
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Diversity and capacity to promote maize growth of bacteria isolated from the Amazon regionBiological nitrogen fixationZea mays L.16S rRNAABSTRACT Maize plants can establish beneficial associations with plant growth-promoting bacteria. However, few studies have been conducted on the characterization and inoculation of these bacteria in the Amazon region. This study aimed to characterize endophytic bacteria isolated from maize in the Amazon region and to assess their capacity to promote plant growth. Fifty-five bacterial isolates were obtained from maize grown in two types of ecosystems, i.e., a cerrado (savanna) and a forest area. The isolates were characterized by the presence of the nifH gene, their ability to synthesize indole-3-acetic acid (IAA) and solubilize calcium phosphate (CaHPO4), and 16S rRNA partial gene sequencing. Twenty-four bacteria contained the nifH gene, of which seven were isolated from maize plants cultivated in a cerrado area and seventeen from a forest area. Fourteen samples showed the capacity to synthesize IAA and only four solubilized calcium phosphate. The following genera were found among these isolates: Pseudomonas; Acinetobacter; Enterobacter; Pantoea; Burkholderia and Bacillus. In addition, eight isolates with plant growth-promoting capacity were selected for a glasshouse experiment involving the inoculation of two maize genotypes (a hybrid and a variety) grown in pots containing soil. Inoculation promoted the development of the maize plants but no significant interaction between maize cultivar and bacterial inoculation was found. A high diversity of endophytic bacteria is present in the Amazon region and these bacteria have potential to promote the development of maize plants.Instituto Nacional de Pesquisas da Amazônia2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672016000200111Acta Amazonica v.46 n.2 2016reponame:Acta Amazonicainstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPA10.1590/1809-4392201502502info:eu-repo/semantics/openAccessSILVA,Krisle daPERIN,LiamaraGOMES,Maria de LourdesBARAÚNA,Alexandre CardosoPEREIRA,Gilmara Maria DuarteMOSQUEIRA,Cátia AparecidaCOSTA,Ismaele Breckenfeld daO'HARA,GrahamZILLI,Jerri Édsoneng2016-03-31T00:00:00Zoai:scielo:S0044-59672016000200111Revistahttps://acta.inpa.gov.br/PUBhttps://old.scielo.br/oai/scielo-oai.phpacta@inpa.gov.br||acta@inpa.gov.br1809-43920044-5967opendoar:2016-03-31T00:00Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.none.fl_str_mv |
Diversity and capacity to promote maize growth of bacteria isolated from the Amazon region |
title |
Diversity and capacity to promote maize growth of bacteria isolated from the Amazon region |
spellingShingle |
Diversity and capacity to promote maize growth of bacteria isolated from the Amazon region SILVA,Krisle da Biological nitrogen fixation Zea mays L. 16S rRNA |
title_short |
Diversity and capacity to promote maize growth of bacteria isolated from the Amazon region |
title_full |
Diversity and capacity to promote maize growth of bacteria isolated from the Amazon region |
title_fullStr |
Diversity and capacity to promote maize growth of bacteria isolated from the Amazon region |
title_full_unstemmed |
Diversity and capacity to promote maize growth of bacteria isolated from the Amazon region |
title_sort |
Diversity and capacity to promote maize growth of bacteria isolated from the Amazon region |
author |
SILVA,Krisle da |
author_facet |
SILVA,Krisle da PERIN,Liamara GOMES,Maria de Lourdes BARAÚNA,Alexandre Cardoso PEREIRA,Gilmara Maria Duarte MOSQUEIRA,Cátia Aparecida COSTA,Ismaele Breckenfeld da O'HARA,Graham ZILLI,Jerri Édson |
author_role |
author |
author2 |
PERIN,Liamara GOMES,Maria de Lourdes BARAÚNA,Alexandre Cardoso PEREIRA,Gilmara Maria Duarte MOSQUEIRA,Cátia Aparecida COSTA,Ismaele Breckenfeld da O'HARA,Graham ZILLI,Jerri Édson |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
SILVA,Krisle da PERIN,Liamara GOMES,Maria de Lourdes BARAÚNA,Alexandre Cardoso PEREIRA,Gilmara Maria Duarte MOSQUEIRA,Cátia Aparecida COSTA,Ismaele Breckenfeld da O'HARA,Graham ZILLI,Jerri Édson |
dc.subject.por.fl_str_mv |
Biological nitrogen fixation Zea mays L. 16S rRNA |
topic |
Biological nitrogen fixation Zea mays L. 16S rRNA |
description |
ABSTRACT Maize plants can establish beneficial associations with plant growth-promoting bacteria. However, few studies have been conducted on the characterization and inoculation of these bacteria in the Amazon region. This study aimed to characterize endophytic bacteria isolated from maize in the Amazon region and to assess their capacity to promote plant growth. Fifty-five bacterial isolates were obtained from maize grown in two types of ecosystems, i.e., a cerrado (savanna) and a forest area. The isolates were characterized by the presence of the nifH gene, their ability to synthesize indole-3-acetic acid (IAA) and solubilize calcium phosphate (CaHPO4), and 16S rRNA partial gene sequencing. Twenty-four bacteria contained the nifH gene, of which seven were isolated from maize plants cultivated in a cerrado area and seventeen from a forest area. Fourteen samples showed the capacity to synthesize IAA and only four solubilized calcium phosphate. The following genera were found among these isolates: Pseudomonas; Acinetobacter; Enterobacter; Pantoea; Burkholderia and Bacillus. In addition, eight isolates with plant growth-promoting capacity were selected for a glasshouse experiment involving the inoculation of two maize genotypes (a hybrid and a variety) grown in pots containing soil. Inoculation promoted the development of the maize plants but no significant interaction between maize cultivar and bacterial inoculation was found. A high diversity of endophytic bacteria is present in the Amazon region and these bacteria have potential to promote the development of maize plants. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672016000200111 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672016000200111 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1809-4392201502502 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Nacional de Pesquisas da Amazônia |
publisher.none.fl_str_mv |
Instituto Nacional de Pesquisas da Amazônia |
dc.source.none.fl_str_mv |
Acta Amazonica v.46 n.2 2016 reponame:Acta Amazonica instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
instname_str |
Instituto Nacional de Pesquisas da Amazônia (INPA) |
instacron_str |
INPA |
institution |
INPA |
reponame_str |
Acta Amazonica |
collection |
Acta Amazonica |
repository.name.fl_str_mv |
Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA) |
repository.mail.fl_str_mv |
acta@inpa.gov.br||acta@inpa.gov.br |
_version_ |
1752129840346038272 |