RFLP pattern determination for the invasive bivalves Limnoperna fortunei (Dunker, 1857) and Corbicula fluminea (Muller, 1774)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Ambiente & Água |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2018000300304 |
Resumo: | Abstract The golden mussel (Limnoperna fortunei) and Corbicula fluminea are considered well-established invasive species in the rivers of Brazil and South America. In addition to the environmental problems resulting from this invasion process, the economic issue, especially in hydroelectric dams, is very worrisome and has mobilized several types of studies on these invasive bivalves. The detection and identification of these organisms in their adult phase in the rivers is not a problem; however, the identification of bivalve larvae by usual morphological methods is difficult due to high similarity conserved in these stages. The use of PCR-RFLP has proven to be an efficient and agile molecular method that allowed the detection of different patterns in the agarose gel for the two bivalves tested. The gel pattern showed a 100 bp band for L. fortunei not detected for C. fluminea. Thus, it is possible to detect larvae of these species from water samples, which can be a powerful tool for environmental monitoring programs on aquatic invasive species. |
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RFLP pattern determination for the invasive bivalves Limnoperna fortunei (Dunker, 1857) and Corbicula fluminea (Muller, 1774)asia clamgolden mussellarvaemolecular identification.Abstract The golden mussel (Limnoperna fortunei) and Corbicula fluminea are considered well-established invasive species in the rivers of Brazil and South America. In addition to the environmental problems resulting from this invasion process, the economic issue, especially in hydroelectric dams, is very worrisome and has mobilized several types of studies on these invasive bivalves. The detection and identification of these organisms in their adult phase in the rivers is not a problem; however, the identification of bivalve larvae by usual morphological methods is difficult due to high similarity conserved in these stages. The use of PCR-RFLP has proven to be an efficient and agile molecular method that allowed the detection of different patterns in the agarose gel for the two bivalves tested. The gel pattern showed a 100 bp band for L. fortunei not detected for C. fluminea. Thus, it is possible to detect larvae of these species from water samples, which can be a powerful tool for environmental monitoring programs on aquatic invasive species.Instituto de Pesquisas Ambientais em Bacias Hidrográficas2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2018000300304Revista Ambiente & Água v.13 n.3 2018reponame:Revista Ambiente & Águainstname:Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI)instacron:IPABHI10.4136/ambi-agua.2172info:eu-repo/semantics/openAccessOliveira Junior,Renato Brito dePaula,Rayan Silva deDiniz,Vinícius Sergio RodriguesCarvalho,Marcela David deCardoso,Antônio Valadãoeng2018-05-15T00:00:00Zoai:scielo:S1980-993X2018000300304Revistahttp://www.ambi-agua.net/PUBhttps://old.scielo.br/oai/scielo-oai.php||ambi.agua@gmail.com1980-993X1980-993Xopendoar:2018-05-15T00:00Revista Ambiente & Água - Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI)false |
dc.title.none.fl_str_mv |
RFLP pattern determination for the invasive bivalves Limnoperna fortunei (Dunker, 1857) and Corbicula fluminea (Muller, 1774) |
title |
RFLP pattern determination for the invasive bivalves Limnoperna fortunei (Dunker, 1857) and Corbicula fluminea (Muller, 1774) |
spellingShingle |
RFLP pattern determination for the invasive bivalves Limnoperna fortunei (Dunker, 1857) and Corbicula fluminea (Muller, 1774) Oliveira Junior,Renato Brito de asia clam golden mussel larvae molecular identification. |
title_short |
RFLP pattern determination for the invasive bivalves Limnoperna fortunei (Dunker, 1857) and Corbicula fluminea (Muller, 1774) |
title_full |
RFLP pattern determination for the invasive bivalves Limnoperna fortunei (Dunker, 1857) and Corbicula fluminea (Muller, 1774) |
title_fullStr |
RFLP pattern determination for the invasive bivalves Limnoperna fortunei (Dunker, 1857) and Corbicula fluminea (Muller, 1774) |
title_full_unstemmed |
RFLP pattern determination for the invasive bivalves Limnoperna fortunei (Dunker, 1857) and Corbicula fluminea (Muller, 1774) |
title_sort |
RFLP pattern determination for the invasive bivalves Limnoperna fortunei (Dunker, 1857) and Corbicula fluminea (Muller, 1774) |
author |
Oliveira Junior,Renato Brito de |
author_facet |
Oliveira Junior,Renato Brito de Paula,Rayan Silva de Diniz,Vinícius Sergio Rodrigues Carvalho,Marcela David de Cardoso,Antônio Valadão |
author_role |
author |
author2 |
Paula,Rayan Silva de Diniz,Vinícius Sergio Rodrigues Carvalho,Marcela David de Cardoso,Antônio Valadão |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Oliveira Junior,Renato Brito de Paula,Rayan Silva de Diniz,Vinícius Sergio Rodrigues Carvalho,Marcela David de Cardoso,Antônio Valadão |
dc.subject.por.fl_str_mv |
asia clam golden mussel larvae molecular identification. |
topic |
asia clam golden mussel larvae molecular identification. |
description |
Abstract The golden mussel (Limnoperna fortunei) and Corbicula fluminea are considered well-established invasive species in the rivers of Brazil and South America. In addition to the environmental problems resulting from this invasion process, the economic issue, especially in hydroelectric dams, is very worrisome and has mobilized several types of studies on these invasive bivalves. The detection and identification of these organisms in their adult phase in the rivers is not a problem; however, the identification of bivalve larvae by usual morphological methods is difficult due to high similarity conserved in these stages. The use of PCR-RFLP has proven to be an efficient and agile molecular method that allowed the detection of different patterns in the agarose gel for the two bivalves tested. The gel pattern showed a 100 bp band for L. fortunei not detected for C. fluminea. Thus, it is possible to detect larvae of these species from water samples, which can be a powerful tool for environmental monitoring programs on aquatic invasive species. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2018000300304 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2018000300304 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.4136/ambi-agua.2172 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Pesquisas Ambientais em Bacias Hidrográficas |
publisher.none.fl_str_mv |
Instituto de Pesquisas Ambientais em Bacias Hidrográficas |
dc.source.none.fl_str_mv |
Revista Ambiente & Água v.13 n.3 2018 reponame:Revista Ambiente & Água instname:Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI) instacron:IPABHI |
instname_str |
Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI) |
instacron_str |
IPABHI |
institution |
IPABHI |
reponame_str |
Revista Ambiente & Água |
collection |
Revista Ambiente & Água |
repository.name.fl_str_mv |
Revista Ambiente & Água - Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI) |
repository.mail.fl_str_mv |
||ambi.agua@gmail.com |
_version_ |
1752129750383460352 |