Gold nanoparticles synthetized with aminolevulinic acid for theranostic

Detalhes bibliográficos
Autor(a) principal: GON??ALVES, KARINA O.
Data de Publicação: 2018
Outros Autores: BONFIM, LETICIA, VIEIRA, DANIEL P., COURROL, LILIA C., PAN AMERICAN CONGRESS OF NANOTECHNOLOGY, 1st; FUNDAMENTALS AND APPLICATIONS TO SHAPE THE FUTURE
Tipo de documento: Artigo de conferência
Título da fonte: Repositório Institucional do IPEN
Texto Completo: http://repositorio.ipen.br/handle/123456789/28432
Resumo: In this study we propose to synthesize gold nanoparticles with 5-ALA, to study cytotoxicity in cell cultures, to perform photodynamic (PDT) and sonodynamic (SDT) therapies and to compare the efficience of 5-ALA delivery. For 5-ALA:AuNPs synthesis, is used a 300 Watt Xenon lamp for photo reduction. Cytotoxicity tests were performed on 96-well plates with THP-1 human cells, which were differentiated into macrophages. For the PDT, an amber LED at 590 nm (~ 100mW) was used for 2 minutes and SDT was used with pulses of 1 MHz and 1W / cm2, for 2 minutes. Cellular viability assays (MTS) were performed to evaluate therapies. The results have shown improved photosensitizer delivery in cells incubated with 5-ALA:AuNPs, leading to cell death and indicating potential use as a photo and sonosensitizer agent.
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spelling 2018-02-01T17:38:44Z2018-02-01T17:38:44ZNovember 27-30, 2017http://repositorio.ipen.br/handle/123456789/28432In this study we propose to synthesize gold nanoparticles with 5-ALA, to study cytotoxicity in cell cultures, to perform photodynamic (PDT) and sonodynamic (SDT) therapies and to compare the efficience of 5-ALA delivery. For 5-ALA:AuNPs synthesis, is used a 300 Watt Xenon lamp for photo reduction. Cytotoxicity tests were performed on 96-well plates with THP-1 human cells, which were differentiated into macrophages. For the PDT, an amber LED at 590 nm (~ 100mW) was used for 2 minutes and SDT was used with pulses of 1 MHz and 1W / cm2, for 2 minutes. Cellular viability assays (MTS) were performed to evaluate therapies. The results have shown improved photosensitizer delivery in cells incubated with 5-ALA:AuNPs, leading to cell death and indicating potential use as a photo and sonosensitizer agent.Submitted by Marco Antonio Oliveira da Silva (maosilva@ipen.br) on 2018-02-01T17:38:44Z No. of bitstreams: 1 24272.pdf: 380774 bytes, checksum: 98d48c75eb394220f1a17c8875e6e826 (MD5)Made available in DSpace on 2018-02-01T17:38:44Z (GMT). No. of bitstreams: 1 24272.pdf: 380774 bytes, checksum: 98d48c75eb394220f1a17c8875e6e826 (MD5)Gold nanoparticles synthetized with aminolevulinic acid for theranosticinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectPanNanoIGuaruj??, SPGON??ALVES, KARINA O.BONFIM, LETICIAVIEIRA, DANIEL P.COURROL, LILIA C.PAN AMERICAN CONGRESS OF NANOTECHNOLOGY, 1st; FUNDAMENTALS AND APPLICATIONS TO SHAPE THE FUTUREinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional do IPENinstname:Instituto de Pesquisas Energéticas e Nucleares (IPEN)instacron:IPEN242722017BONFIM, LETICIAVIEIRA, DANIEL P.18-02Proceedings141853158photodynamic and sonodynamic therapiesBONFIM, LETICIA:14185:810:NVIEIRA, DANIEL P.:3158:810:NORIGINAL24272.pdf24272.pdfapplication/pdf380774http://repositorio.ipen.br/bitstream/123456789/28432/1/24272.pdf98d48c75eb394220f1a17c8875e6e826MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.ipen.br/bitstream/123456789/28432/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/284322020-09-29 16:43:15.368oai:repositorio.ipen.br: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Repositório InstitucionalPUBhttp://repositorio.ipen.br/oai/requestbibl@ipen.bropendoar:45102020-09-29T16:43:15Repositório Institucional do IPEN - Instituto de Pesquisas Energéticas e Nucleares (IPEN)false
dc.title.pt_BR.fl_str_mv Gold nanoparticles synthetized with aminolevulinic acid for theranostic
title Gold nanoparticles synthetized with aminolevulinic acid for theranostic
spellingShingle Gold nanoparticles synthetized with aminolevulinic acid for theranostic
GON??ALVES, KARINA O.
title_short Gold nanoparticles synthetized with aminolevulinic acid for theranostic
title_full Gold nanoparticles synthetized with aminolevulinic acid for theranostic
title_fullStr Gold nanoparticles synthetized with aminolevulinic acid for theranostic
title_full_unstemmed Gold nanoparticles synthetized with aminolevulinic acid for theranostic
title_sort Gold nanoparticles synthetized with aminolevulinic acid for theranostic
author GON??ALVES, KARINA O.
author_facet GON??ALVES, KARINA O.
BONFIM, LETICIA
VIEIRA, DANIEL P.
COURROL, LILIA C.
PAN AMERICAN CONGRESS OF NANOTECHNOLOGY, 1st; FUNDAMENTALS AND APPLICATIONS TO SHAPE THE FUTURE
author_role author
author2 BONFIM, LETICIA
VIEIRA, DANIEL P.
COURROL, LILIA C.
PAN AMERICAN CONGRESS OF NANOTECHNOLOGY, 1st; FUNDAMENTALS AND APPLICATIONS TO SHAPE THE FUTURE
author2_role author
author
author
author
dc.contributor.author.fl_str_mv GON??ALVES, KARINA O.
BONFIM, LETICIA
VIEIRA, DANIEL P.
COURROL, LILIA C.
PAN AMERICAN CONGRESS OF NANOTECHNOLOGY, 1st; FUNDAMENTALS AND APPLICATIONS TO SHAPE THE FUTURE
description In this study we propose to synthesize gold nanoparticles with 5-ALA, to study cytotoxicity in cell cultures, to perform photodynamic (PDT) and sonodynamic (SDT) therapies and to compare the efficience of 5-ALA delivery. For 5-ALA:AuNPs synthesis, is used a 300 Watt Xenon lamp for photo reduction. Cytotoxicity tests were performed on 96-well plates with THP-1 human cells, which were differentiated into macrophages. For the PDT, an amber LED at 590 nm (~ 100mW) was used for 2 minutes and SDT was used with pulses of 1 MHz and 1W / cm2, for 2 minutes. Cellular viability assays (MTS) were performed to evaluate therapies. The results have shown improved photosensitizer delivery in cells incubated with 5-ALA:AuNPs, leading to cell death and indicating potential use as a photo and sonosensitizer agent.
publishDate 2018
dc.date.evento.pt_BR.fl_str_mv November 27-30, 2017
dc.date.accessioned.fl_str_mv 2018-02-01T17:38:44Z
dc.date.available.fl_str_mv 2018-02-01T17:38:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://repositorio.ipen.br/handle/123456789/28432
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