In vitro study of the microstructural effects of photodynamic therapy in medical supplies when used for disinfection

• Autor(a) principal: NAMBA, A.F.
• Data de Publicação: 2020
• Outros Autores: DEL-VALLE, M., DAGHASTANLI, N.A., ANA, P.A., BRAZILIAN CONGRESS IN BIOMEDICAL ENGINEERING, 27th
• Tipo de documento: Artigo de conferência
• Título da fonte: Repositório Institucional do IPEN
• Texto Completo: http://200.136.52.105/handle/123456789/31682
• topic: photodynamic therapy; sterilization; medical supplies; lasers

Cleaning and disinfecting surfaces and materials in health services are primary elements in infection control measures. For thermosensitive materials, the chemical agents used have disadvantages such as the odor of the products, which can cause allergic reactions to patients and the nursing staff. Photodynamic therapy (PDT) has been shown to be an effective technique in the treatment of infections caused by different microorganisms; however, nothing is known about the effects of this technique on the microstructure of hospital supplies. This in vitro study aimed to evaluate the effects of 0.2% peracetic acid, 1% sodium hypochlorite and PDT with 0.01% methylene blue on the composition and color changes of hospital masks and extensions. For this purpose, 100 mask samples and 100 extension samples were randomly distributed in 20 experimental groups (n = 10, 10 groups for each material), in which the applied substance was varied (sodium hypochlorite, peracetic acid and PDT) and the number of applications (without application, 1, 2 or 3 applications). The compositional analysis was performed by Fourier transform infrared spectroscopy, while the color changes were evaluated using image analysis by CIElab method evaluating the parameters L*, a*, b* and ???E. The statistical analysis was performed at 5% significance level. It was observed that all agents altered the composition of the materials in a similar way. Although all agents promoted changes in different parameters evaluated, peracetic acid and methylene blue alone altered the final color perceived only in extensions. It was concluded that 0.2% peracetic acid, 1% sodium hypochlorite and PDT alter the chemical composition of both masks and extensions, and that such changes have a positive relationship with the number of treatments performed. These compositional changes may be related to the color changes promoted in both materials by all agents tested.