The Annatto Carotenoids and the Norbixin Absorption Coefficient
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório do Instituto de Tecnologia de Alimentos |
Texto Completo: | http://repositorio.ital.sp.gov.br/jspui/handle/123456789/228 |
Resumo: | Annatto seeds present cis-bixin as the major carotenoids, but the norbixin salt is the main pigment present in dyes obtained from the alkaline extraction process. For analyzing the norbixin, the absorptions are obtained in the two spectral peaks with higher intensity, but discrepancies in the published extinction values have led to serious doubts. Taking into account the use of absorption coefficient for evaluating the norbixin concentration in annatto seeds and extracts, the present study evaluated the absorption coefficient in various solvents and the total uncertainty associated with this value was determined. The norbixin standard was prepared from annatto seeds, purified by column chromatography and the purity of norbixin was evaluated by HPLC-DAD. The absorption coefficients were determined for norbixin in different solvents and the uncertainty was evaluated. The 0.5% potassium hydroxide, the main solvent used for marketing the annatto seeds and extracts, showed the absorption coefficients of 2887 at 454nm and of 2546 at 483nm, and the estimation of expanded uncertainty (K=2) was ±86 and 85 g 100 mL-1, respectively. |
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The Annatto Carotenoids and the Norbixin Absorption CoefficientBixa orellanaPigmentsSolventsQuantificationAnnatto seeds present cis-bixin as the major carotenoids, but the norbixin salt is the main pigment present in dyes obtained from the alkaline extraction process. For analyzing the norbixin, the absorptions are obtained in the two spectral peaks with higher intensity, but discrepancies in the published extinction values have led to serious doubts. Taking into account the use of absorption coefficient for evaluating the norbixin concentration in annatto seeds and extracts, the present study evaluated the absorption coefficient in various solvents and the total uncertainty associated with this value was determined. The norbixin standard was prepared from annatto seeds, purified by column chromatography and the purity of norbixin was evaluated by HPLC-DAD. The absorption coefficients were determined for norbixin in different solvents and the uncertainty was evaluated. The 0.5% potassium hydroxide, the main solvent used for marketing the annatto seeds and extracts, showed the absorption coefficients of 2887 at 454nm and of 2546 at 483nm, and the estimation of expanded uncertainty (K=2) was ±86 and 85 g 100 mL-1, respectively.Silva, Marta Gomes da; et. al.2021-12-23T17:16:03Z2021-12-23T17:16:03Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfRevista do Instituto Adolfo Lutz, v. 77, 2018.http://repositorio.ital.sp.gov.br/jspui/handle/123456789/228reponame:Repositório do Instituto de Tecnologia de Alimentosinstname:Instituto de Tecnologia de Alimentos (ITAL)instacron:ITALenginfo:eu-repo/semantics/openAccess2022-05-20T16:13:49Zoai:http://repositorio.ital.sp.gov.br:123456789/228Repositório InstitucionalPUBhttp://repositorio.ital.sp.gov.br/oai/requestbjftsec@ital.sp.gov.br || bjftsec@ital.sp.gov.bropendoar:2022-05-20T16:13:49Repositório do Instituto de Tecnologia de Alimentos - Instituto de Tecnologia de Alimentos (ITAL)false |
dc.title.none.fl_str_mv |
The Annatto Carotenoids and the Norbixin Absorption Coefficient |
title |
The Annatto Carotenoids and the Norbixin Absorption Coefficient |
spellingShingle |
The Annatto Carotenoids and the Norbixin Absorption Coefficient Silva, Marta Gomes da; et. al. Bixa orellana Pigments Solvents Quantification |
title_short |
The Annatto Carotenoids and the Norbixin Absorption Coefficient |
title_full |
The Annatto Carotenoids and the Norbixin Absorption Coefficient |
title_fullStr |
The Annatto Carotenoids and the Norbixin Absorption Coefficient |
title_full_unstemmed |
The Annatto Carotenoids and the Norbixin Absorption Coefficient |
title_sort |
The Annatto Carotenoids and the Norbixin Absorption Coefficient |
author |
Silva, Marta Gomes da; et. al. |
author_facet |
Silva, Marta Gomes da; et. al. |
author_role |
author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Silva, Marta Gomes da; et. al. |
dc.subject.none.fl_str_mv |
|
dc.subject.por.fl_str_mv |
Bixa orellana Pigments Solvents Quantification |
topic |
Bixa orellana Pigments Solvents Quantification |
description |
Annatto seeds present cis-bixin as the major carotenoids, but the norbixin salt is the main pigment present in dyes obtained from the alkaline extraction process. For analyzing the norbixin, the absorptions are obtained in the two spectral peaks with higher intensity, but discrepancies in the published extinction values have led to serious doubts. Taking into account the use of absorption coefficient for evaluating the norbixin concentration in annatto seeds and extracts, the present study evaluated the absorption coefficient in various solvents and the total uncertainty associated with this value was determined. The norbixin standard was prepared from annatto seeds, purified by column chromatography and the purity of norbixin was evaluated by HPLC-DAD. The absorption coefficients were determined for norbixin in different solvents and the uncertainty was evaluated. The 0.5% potassium hydroxide, the main solvent used for marketing the annatto seeds and extracts, showed the absorption coefficients of 2887 at 454nm and of 2546 at 483nm, and the estimation of expanded uncertainty (K=2) was ±86 and 85 g 100 mL-1, respectively. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2021-12-23T17:16:03Z 2021-12-23T17:16:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
|
dc.identifier.uri.fl_str_mv |
Revista do Instituto Adolfo Lutz, v. 77, 2018. http://repositorio.ital.sp.gov.br/jspui/handle/123456789/228 |
identifier_str_mv |
Revista do Instituto Adolfo Lutz, v. 77, 2018. |
url |
http://repositorio.ital.sp.gov.br/jspui/handle/123456789/228 |
dc.language.none.fl_str_mv |
|
dc.language.iso.fl_str_mv |
eng |
language_invalid_str_mv |
|
language |
eng |
dc.rights.none.fl_str_mv |
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dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
|
publisher.none.fl_str_mv |
|
dc.source.none.fl_str_mv |
reponame:Repositório do Instituto de Tecnologia de Alimentos instname:Instituto de Tecnologia de Alimentos (ITAL) instacron:ITAL |
instname_str |
Instituto de Tecnologia de Alimentos (ITAL) |
instacron_str |
ITAL |
institution |
ITAL |
reponame_str |
Repositório do Instituto de Tecnologia de Alimentos |
collection |
Repositório do Instituto de Tecnologia de Alimentos |
repository.name.fl_str_mv |
Repositório do Instituto de Tecnologia de Alimentos - Instituto de Tecnologia de Alimentos (ITAL) |
repository.mail.fl_str_mv |
bjftsec@ital.sp.gov.br || bjftsec@ital.sp.gov.br |
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