Brazil nuts are subject to infection with B and G aflatoxin-producing fungus, Aspergillus pseudonomius

Detalhes bibliográficos
Autor(a) principal: Massi, Fernanda Pelisson; et. al.
Data de Publicação: 2014
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório do Instituto de Tecnologia de Alimentos
Texto Completo: http://repositorio.ital.sp.gov.br/jspui/handle/123456789/378
Resumo: The exploitation of the Brazil nut is one of the most important activities of the extractive communities of the Amazon rainforest. However, its commercialization can be affected by the presence of aflatoxins produced by fungi, namely Aspergillus section Flavi. In the present study, we investigated a collection of Aspergillus nomius strains isolated from Brazil nuts using different approaches, including morphological characters, RAPD and AFLP profiles, partial β-tubulin and calmodulin nucleotide sequences, aflatoxin patterns, as well as tolerance to low water activity in cultured media. Results showed that most of the isolates do belong to A. nomius species, but a few were re-identified as Aspergillus pseudonomius, a very recently described species. The results of the analyses of molecular variance, as well as the high pairwise FST values between A. nomius and A. pseudonomius suggested the isolation between these two species and the inexistence of gene flow. Fixed interspecific nucleotide polymorphisms at β-tubulin and calmodulin loci are presented. All A. pseudonomius strains analyzed produced aflatoxins AFB1, AFB2, AFG1 and AFG2. This study contains the first-ever report on the occurrence in Brazil nuts of A. pseudonomius. The G-type aflatoxins and the mycotoxin tenuazonic acid are reported here for the first time in A. pseudonomius. © 2014 Elsevier B.V. All rights reserved.
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spelling Brazil nuts are subject to infection with B and G aflatoxin-producing fungus, Aspergillus pseudonomiusAspergillus section FlaviBrazil nutAflatoxin productionThe exploitation of the Brazil nut is one of the most important activities of the extractive communities of the Amazon rainforest. However, its commercialization can be affected by the presence of aflatoxins produced by fungi, namely Aspergillus section Flavi. In the present study, we investigated a collection of Aspergillus nomius strains isolated from Brazil nuts using different approaches, including morphological characters, RAPD and AFLP profiles, partial β-tubulin and calmodulin nucleotide sequences, aflatoxin patterns, as well as tolerance to low water activity in cultured media. Results showed that most of the isolates do belong to A. nomius species, but a few were re-identified as Aspergillus pseudonomius, a very recently described species. The results of the analyses of molecular variance, as well as the high pairwise FST values between A. nomius and A. pseudonomius suggested the isolation between these two species and the inexistence of gene flow. Fixed interspecific nucleotide polymorphisms at β-tubulin and calmodulin loci are presented. All A. pseudonomius strains analyzed produced aflatoxins AFB1, AFB2, AFG1 and AFG2. This study contains the first-ever report on the occurrence in Brazil nuts of A. pseudonomius. The G-type aflatoxins and the mycotoxin tenuazonic acid are reported here for the first time in A. pseudonomius. © 2014 Elsevier B.V. All rights reserved.ElsevierMassi, Fernanda Pelisson; et. al.2022-08-01T19:39:57Z2022-08-01T19:39:57Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfInternational Journal of Food Microbiology, New York, v.186, p.14-21, março/2014.http://repositorio.ital.sp.gov.br/jspui/handle/123456789/378reponame:Repositório do Instituto de Tecnologia de Alimentosinstname:Instituto de Tecnologia de Alimentos (ITAL)instacron:ITALenginfo:eu-repo/semantics/openAccess2022-08-01T19:39:57Zoai:http://repositorio.ital.sp.gov.br:123456789/378Repositório InstitucionalPUBhttp://repositorio.ital.sp.gov.br/oai/requestbjftsec@ital.sp.gov.br || bjftsec@ital.sp.gov.bropendoar:2022-08-01T19:39:57Repositório do Instituto de Tecnologia de Alimentos - Instituto de Tecnologia de Alimentos (ITAL)false
dc.title.none.fl_str_mv Brazil nuts are subject to infection with B and G aflatoxin-producing fungus, Aspergillus pseudonomius
title Brazil nuts are subject to infection with B and G aflatoxin-producing fungus, Aspergillus pseudonomius
spellingShingle Brazil nuts are subject to infection with B and G aflatoxin-producing fungus, Aspergillus pseudonomius
Massi, Fernanda Pelisson; et. al.
Aspergillus section Flavi
Brazil nut
Aflatoxin production
title_short Brazil nuts are subject to infection with B and G aflatoxin-producing fungus, Aspergillus pseudonomius
title_full Brazil nuts are subject to infection with B and G aflatoxin-producing fungus, Aspergillus pseudonomius
title_fullStr Brazil nuts are subject to infection with B and G aflatoxin-producing fungus, Aspergillus pseudonomius
title_full_unstemmed Brazil nuts are subject to infection with B and G aflatoxin-producing fungus, Aspergillus pseudonomius
title_sort Brazil nuts are subject to infection with B and G aflatoxin-producing fungus, Aspergillus pseudonomius
author Massi, Fernanda Pelisson; et. al.
author_facet Massi, Fernanda Pelisson; et. al.
author_role author
dc.contributor.none.fl_str_mv







dc.contributor.author.fl_str_mv Massi, Fernanda Pelisson; et. al.
dc.subject.none.fl_str_mv

dc.subject.por.fl_str_mv Aspergillus section Flavi
Brazil nut
Aflatoxin production
topic Aspergillus section Flavi
Brazil nut
Aflatoxin production
description The exploitation of the Brazil nut is one of the most important activities of the extractive communities of the Amazon rainforest. However, its commercialization can be affected by the presence of aflatoxins produced by fungi, namely Aspergillus section Flavi. In the present study, we investigated a collection of Aspergillus nomius strains isolated from Brazil nuts using different approaches, including morphological characters, RAPD and AFLP profiles, partial β-tubulin and calmodulin nucleotide sequences, aflatoxin patterns, as well as tolerance to low water activity in cultured media. Results showed that most of the isolates do belong to A. nomius species, but a few were re-identified as Aspergillus pseudonomius, a very recently described species. The results of the analyses of molecular variance, as well as the high pairwise FST values between A. nomius and A. pseudonomius suggested the isolation between these two species and the inexistence of gene flow. Fixed interspecific nucleotide polymorphisms at β-tubulin and calmodulin loci are presented. All A. pseudonomius strains analyzed produced aflatoxins AFB1, AFB2, AFG1 and AFG2. This study contains the first-ever report on the occurrence in Brazil nuts of A. pseudonomius. The G-type aflatoxins and the mycotoxin tenuazonic acid are reported here for the first time in A. pseudonomius. © 2014 Elsevier B.V. All rights reserved.
publishDate 2014
dc.date.none.fl_str_mv




2014
2022-08-01T19:39:57Z
2022-08-01T19:39:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv International Journal of Food Microbiology, New York, v.186, p.14-21, março/2014.
http://repositorio.ital.sp.gov.br/jspui/handle/123456789/378
identifier_str_mv
International Journal of Food Microbiology, New York, v.186, p.14-21, março/2014.
url http://repositorio.ital.sp.gov.br/jspui/handle/123456789/378
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dc.language.iso.fl_str_mv eng
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application/pdf
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Elsevier
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Elsevier
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