Low-cost, specific PCR assays to identify the main aflatoxigenic species of Aspergillus section Flavi

Detalhes bibliográficos
Autor(a) principal: Silva, Josué J.; et al.
Data de Publicação: 2022
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório do Instituto de Tecnologia de Alimentos
Texto Completo: http://repositorio.ital.sp.gov.br/jspui/handle/123456789/430
Resumo: Aflatoxins are fungal metabolites that are present as contaminants in food globally. Most aflatoxigenic species belong to Aspergillus section Flavi, and the main ones are grouped in the A. flavus clade, where many cryptic species that are difficult to discriminate are found. In this study, we investigated inter- and intraspecific diversity of the A. flavus clade to develop low-cost, species-specific PCR assays for identifying aflatoxigenic species. A total of 269 sequences of the second largest subunit of RNA polymerase II (RPB2) locus were retrieved from GenBank, and primer pairs were designed using data mining to identify A. flavus, A. parasiticus, and A. novoparasiticus. Species-specific amplicons of approximately 620, 350, and 860 bp enabled identification of target species as A. flavus, A. parasiticus, and A. novoparasiticus, respectively.
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spelling Low-cost, specific PCR assays to identify the main aflatoxigenic species of Aspergillus section FlaviAspergillus novoparasiticusAspergillus section FlaviAflatoxigenicSpecies-specificRPB2Aflatoxins are fungal metabolites that are present as contaminants in food globally. Most aflatoxigenic species belong to Aspergillus section Flavi, and the main ones are grouped in the A. flavus clade, where many cryptic species that are difficult to discriminate are found. In this study, we investigated inter- and intraspecific diversity of the A. flavus clade to develop low-cost, species-specific PCR assays for identifying aflatoxigenic species. A total of 269 sequences of the second largest subunit of RNA polymerase II (RPB2) locus were retrieved from GenBank, and primer pairs were designed using data mining to identify A. flavus, A. parasiticus, and A. novoparasiticus. Species-specific amplicons of approximately 620, 350, and 860 bp enabled identification of target species as A. flavus, A. parasiticus, and A. novoparasiticus, respectively.Silva, Josué J.; et al.2022-09-02T17:12:33Z2022-09-02T17:12:33Z2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfJournal of Microbiological Methods, v. 196, 106470, 2022.http://repositorio.ital.sp.gov.br/jspui/handle/123456789/430reponame:Repositório do Instituto de Tecnologia de Alimentosinstname:Instituto de Tecnologia de Alimentos (ITAL)instacron:ITALenginfo:eu-repo/semantics/openAccess2022-09-02T17:12:33Zoai:http://repositorio.ital.sp.gov.br:123456789/430Repositório InstitucionalPUBhttp://repositorio.ital.sp.gov.br/oai/requestbjftsec@ital.sp.gov.br || bjftsec@ital.sp.gov.bropendoar:2022-09-02T17:12:33Repositório do Instituto de Tecnologia de Alimentos - Instituto de Tecnologia de Alimentos (ITAL)false
dc.title.none.fl_str_mv Low-cost, specific PCR assays to identify the main aflatoxigenic species of Aspergillus section Flavi
title Low-cost, specific PCR assays to identify the main aflatoxigenic species of Aspergillus section Flavi
spellingShingle Low-cost, specific PCR assays to identify the main aflatoxigenic species of Aspergillus section Flavi
Silva, Josué J.; et al.
Aspergillus novoparasiticus
Aspergillus section Flavi
Aflatoxigenic
Species-specific
RPB2
title_short Low-cost, specific PCR assays to identify the main aflatoxigenic species of Aspergillus section Flavi
title_full Low-cost, specific PCR assays to identify the main aflatoxigenic species of Aspergillus section Flavi
title_fullStr Low-cost, specific PCR assays to identify the main aflatoxigenic species of Aspergillus section Flavi
title_full_unstemmed Low-cost, specific PCR assays to identify the main aflatoxigenic species of Aspergillus section Flavi
title_sort Low-cost, specific PCR assays to identify the main aflatoxigenic species of Aspergillus section Flavi
author Silva, Josué J.; et al.
author_facet Silva, Josué J.; et al.
author_role author
dc.contributor.none.fl_str_mv







dc.contributor.author.fl_str_mv Silva, Josué J.; et al.
dc.subject.none.fl_str_mv

dc.subject.por.fl_str_mv Aspergillus novoparasiticus
Aspergillus section Flavi
Aflatoxigenic
Species-specific
RPB2
topic Aspergillus novoparasiticus
Aspergillus section Flavi
Aflatoxigenic
Species-specific
RPB2
description Aflatoxins are fungal metabolites that are present as contaminants in food globally. Most aflatoxigenic species belong to Aspergillus section Flavi, and the main ones are grouped in the A. flavus clade, where many cryptic species that are difficult to discriminate are found. In this study, we investigated inter- and intraspecific diversity of the A. flavus clade to develop low-cost, species-specific PCR assays for identifying aflatoxigenic species. A total of 269 sequences of the second largest subunit of RNA polymerase II (RPB2) locus were retrieved from GenBank, and primer pairs were designed using data mining to identify A. flavus, A. parasiticus, and A. novoparasiticus. Species-specific amplicons of approximately 620, 350, and 860 bp enabled identification of target species as A. flavus, A. parasiticus, and A. novoparasiticus, respectively.
publishDate 2022
dc.date.none.fl_str_mv




2022-09-02T17:12:33Z
2022-09-02T17:12:33Z
2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv

dc.identifier.uri.fl_str_mv Journal of Microbiological Methods, v. 196, 106470, 2022.
http://repositorio.ital.sp.gov.br/jspui/handle/123456789/430
identifier_str_mv
Journal of Microbiological Methods, v. 196, 106470, 2022.
url http://repositorio.ital.sp.gov.br/jspui/handle/123456789/430
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dc.language.iso.fl_str_mv eng
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language eng
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eu_rights_str_mv openAccess
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application/pdf
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reponame:Repositório do Instituto de Tecnologia de Alimentos
instname:Instituto de Tecnologia de Alimentos (ITAL)
instacron:ITAL
instname_str Instituto de Tecnologia de Alimentos (ITAL)
instacron_str ITAL
institution ITAL
reponame_str Repositório do Instituto de Tecnologia de Alimentos
collection Repositório do Instituto de Tecnologia de Alimentos
repository.name.fl_str_mv Repositório do Instituto de Tecnologia de Alimentos - Instituto de Tecnologia de Alimentos (ITAL)
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