Influence of macronutrients, sucrose and LED on in vitro culture of Lomatozona artemisiifolia (Asteraceae - Eupatorieae)
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Rodriguésia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602021000100242 |
Resumo: | Abstract Balanced levels of macronutrients and sucrose may ensure the success of micropropagation of the endangered ones. This study aimed to evaluate the effect of levels of salts in the culture medium on in vitro culture of Lomatozona artemisiifolia, as well as to determine the influence of light emitting diode (LED) on the shoot proliferation and rooting of the species. Nodal segments were used to evaluate the different macronutrient concentrations of MS medium (25, 50 and 100%), as well as sucrose concentrations (0.0 mM; 0.34 mM; 0.68 mM and 1.03 mM). Five light conditions were evaluated at shoots proliferation and rooting [100% blue (455 nm); 100% red (630 nm); 30% blue + 70% red; 30% red + 70% blue or fluorescent white]. Low levels of macronutrients in MS (50% and 25%) and sucrose (0.00 mM and 0.34 mM) resulted in plants with higher height, number of shoots and higher production of photosynthetic pigments. The 100% red light promoted rooting of 100% of the plants, and in 100% red or 70% red + 30% blue, higher plants were observed. These results demonstrate that under in vitro conditions, L. artemisiifolia has low nutritional needs, typical of plants that live in rupestrian fields. |
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Influence of macronutrients, sucrose and LED on in vitro culture of Lomatozona artemisiifolia (Asteraceae - Eupatorieae)culture mediummicropropagationrock plantsspectral lightsucrose concentrationAbstract Balanced levels of macronutrients and sucrose may ensure the success of micropropagation of the endangered ones. This study aimed to evaluate the effect of levels of salts in the culture medium on in vitro culture of Lomatozona artemisiifolia, as well as to determine the influence of light emitting diode (LED) on the shoot proliferation and rooting of the species. Nodal segments were used to evaluate the different macronutrient concentrations of MS medium (25, 50 and 100%), as well as sucrose concentrations (0.0 mM; 0.34 mM; 0.68 mM and 1.03 mM). Five light conditions were evaluated at shoots proliferation and rooting [100% blue (455 nm); 100% red (630 nm); 30% blue + 70% red; 30% red + 70% blue or fluorescent white]. Low levels of macronutrients in MS (50% and 25%) and sucrose (0.00 mM and 0.34 mM) resulted in plants with higher height, number of shoots and higher production of photosynthetic pigments. The 100% red light promoted rooting of 100% of the plants, and in 100% red or 70% red + 30% blue, higher plants were observed. These results demonstrate that under in vitro conditions, L. artemisiifolia has low nutritional needs, typical of plants that live in rupestrian fields.Instituto de Pesquisas Jardim Botânico do Rio de Janeiro2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602021000100242Rodriguésia v.72 2021reponame:Rodriguésia (Online)instname:Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ)instacron:JBRJ10.1590/2175-7860202172054info:eu-repo/semantics/openAccessSilva,Lívia Cristina daMoreira,Marlene PiresSilveira,Andreia Alves da CostaSibov,Sérgio Tadeueng2021-07-06T00:00:00Zoai:scielo:S2175-78602021000100242Revistahttp://rodriguesia.jbrj.gov.br/https://old.scielo.br/oai/scielo-oai.phprodriguesia@jbrj.gov.br2175-78600370-6583opendoar:2021-07-06T00:00Rodriguésia (Online) - Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ)false |
dc.title.none.fl_str_mv |
Influence of macronutrients, sucrose and LED on in vitro culture of Lomatozona artemisiifolia (Asteraceae - Eupatorieae) |
title |
Influence of macronutrients, sucrose and LED on in vitro culture of Lomatozona artemisiifolia (Asteraceae - Eupatorieae) |
spellingShingle |
Influence of macronutrients, sucrose and LED on in vitro culture of Lomatozona artemisiifolia (Asteraceae - Eupatorieae) Silva,Lívia Cristina da culture medium micropropagation rock plants spectral light sucrose concentration |
title_short |
Influence of macronutrients, sucrose and LED on in vitro culture of Lomatozona artemisiifolia (Asteraceae - Eupatorieae) |
title_full |
Influence of macronutrients, sucrose and LED on in vitro culture of Lomatozona artemisiifolia (Asteraceae - Eupatorieae) |
title_fullStr |
Influence of macronutrients, sucrose and LED on in vitro culture of Lomatozona artemisiifolia (Asteraceae - Eupatorieae) |
title_full_unstemmed |
Influence of macronutrients, sucrose and LED on in vitro culture of Lomatozona artemisiifolia (Asteraceae - Eupatorieae) |
title_sort |
Influence of macronutrients, sucrose and LED on in vitro culture of Lomatozona artemisiifolia (Asteraceae - Eupatorieae) |
author |
Silva,Lívia Cristina da |
author_facet |
Silva,Lívia Cristina da Moreira,Marlene Pires Silveira,Andreia Alves da Costa Sibov,Sérgio Tadeu |
author_role |
author |
author2 |
Moreira,Marlene Pires Silveira,Andreia Alves da Costa Sibov,Sérgio Tadeu |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Silva,Lívia Cristina da Moreira,Marlene Pires Silveira,Andreia Alves da Costa Sibov,Sérgio Tadeu |
dc.subject.por.fl_str_mv |
culture medium micropropagation rock plants spectral light sucrose concentration |
topic |
culture medium micropropagation rock plants spectral light sucrose concentration |
description |
Abstract Balanced levels of macronutrients and sucrose may ensure the success of micropropagation of the endangered ones. This study aimed to evaluate the effect of levels of salts in the culture medium on in vitro culture of Lomatozona artemisiifolia, as well as to determine the influence of light emitting diode (LED) on the shoot proliferation and rooting of the species. Nodal segments were used to evaluate the different macronutrient concentrations of MS medium (25, 50 and 100%), as well as sucrose concentrations (0.0 mM; 0.34 mM; 0.68 mM and 1.03 mM). Five light conditions were evaluated at shoots proliferation and rooting [100% blue (455 nm); 100% red (630 nm); 30% blue + 70% red; 30% red + 70% blue or fluorescent white]. Low levels of macronutrients in MS (50% and 25%) and sucrose (0.00 mM and 0.34 mM) resulted in plants with higher height, number of shoots and higher production of photosynthetic pigments. The 100% red light promoted rooting of 100% of the plants, and in 100% red or 70% red + 30% blue, higher plants were observed. These results demonstrate that under in vitro conditions, L. artemisiifolia has low nutritional needs, typical of plants that live in rupestrian fields. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602021000100242 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602021000100242 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/2175-7860202172054 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Pesquisas Jardim Botânico do Rio de Janeiro |
publisher.none.fl_str_mv |
Instituto de Pesquisas Jardim Botânico do Rio de Janeiro |
dc.source.none.fl_str_mv |
Rodriguésia v.72 2021 reponame:Rodriguésia (Online) instname:Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ) instacron:JBRJ |
instname_str |
Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ) |
instacron_str |
JBRJ |
institution |
JBRJ |
reponame_str |
Rodriguésia (Online) |
collection |
Rodriguésia (Online) |
repository.name.fl_str_mv |
Rodriguésia (Online) - Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ) |
repository.mail.fl_str_mv |
rodriguesia@jbrj.gov.br |
_version_ |
1752128674471084032 |