Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro

Detalhes bibliográficos
Autor(a) principal: Ferreira,Wagner de Melo
Data de Publicação: 2018
Outros Autores: Oliveira,Sidney Pereira de, Suzuki,Rogério Mamoru, Silva,Kellen Lagares Ferreira, Soares Júnior,Jack Wild Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Rodriguésia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602018000402137
Resumo: Abstract Catasetum macrocarpum is an epiphytic orchid that has been subjected to strong environmental pressure in the state of Tocantins. This investigation aimed at studying the germination, growth and morpho-anatomical development of C. macrocarpum under in vitro conditions. The effects of three culture media on the in vitro germination and on the multiplication and growth of 90-day-old seedlings were studied: Murashige & Skoog (full- and half-strength), Knudson C, and Vacin & Went. The effects of different concentrations of benzyladenine (BA) and naphthaleneacetic acid (NAA) on the multiplication and growth of 120-day-old plants were evaluated. Anatomical studies were conducted on protocorms at different developmental stages. Acclimatization was also carried out. Knudson C was the best medium for seed germination whereas Vacin & Went promoted the greatest protocorm development. Half-strength Murashige & Skoog was the most effective medium for seedling multiplication and growth. The results revealed that 1 mg L-1 BA was the best treatment for shoot proliferation and leaf production. NAA at 0.5 mg L-1 strongly favored root formation. The anatomical study revealed that the early stages of C. macrocarpum development do not always coincide with the morphological phases described. The acclimatization of C. macrocarpum plants provided successful results regarding plant survival.
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spelling Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitroacclimatizationculture mediagrowth regulatorshistological studyorchidAbstract Catasetum macrocarpum is an epiphytic orchid that has been subjected to strong environmental pressure in the state of Tocantins. This investigation aimed at studying the germination, growth and morpho-anatomical development of C. macrocarpum under in vitro conditions. The effects of three culture media on the in vitro germination and on the multiplication and growth of 90-day-old seedlings were studied: Murashige & Skoog (full- and half-strength), Knudson C, and Vacin & Went. The effects of different concentrations of benzyladenine (BA) and naphthaleneacetic acid (NAA) on the multiplication and growth of 120-day-old plants were evaluated. Anatomical studies were conducted on protocorms at different developmental stages. Acclimatization was also carried out. Knudson C was the best medium for seed germination whereas Vacin & Went promoted the greatest protocorm development. Half-strength Murashige & Skoog was the most effective medium for seedling multiplication and growth. The results revealed that 1 mg L-1 BA was the best treatment for shoot proliferation and leaf production. NAA at 0.5 mg L-1 strongly favored root formation. The anatomical study revealed that the early stages of C. macrocarpum development do not always coincide with the morphological phases described. The acclimatization of C. macrocarpum plants provided successful results regarding plant survival.Instituto de Pesquisas Jardim Botânico do Rio de Janeiro2018-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602018000402137Rodriguésia v.69 n.4 2018reponame:Rodriguésia (Online)instname:Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ)instacron:JBRJ10.1590/2175-7860201869442info:eu-repo/semantics/openAccessFerreira,Wagner de MeloOliveira,Sidney Pereira deSuzuki,Rogério MamoruSilva,Kellen Lagares FerreiraSoares Júnior,Jack Wild Pereiraeng2018-12-14T00:00:00Zoai:scielo:S2175-78602018000402137Revistahttp://rodriguesia.jbrj.gov.br/https://old.scielo.br/oai/scielo-oai.phprodriguesia@jbrj.gov.br2175-78600370-6583opendoar:2018-12-14T00:00Rodriguésia (Online) - Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ)false
dc.title.none.fl_str_mv Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro
title Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro
spellingShingle Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro
Ferreira,Wagner de Melo
acclimatization
culture media
growth regulators
histological study
orchid
title_short Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro
title_full Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro
title_fullStr Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro
title_full_unstemmed Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro
title_sort Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro
author Ferreira,Wagner de Melo
author_facet Ferreira,Wagner de Melo
Oliveira,Sidney Pereira de
Suzuki,Rogério Mamoru
Silva,Kellen Lagares Ferreira
Soares Júnior,Jack Wild Pereira
author_role author
author2 Oliveira,Sidney Pereira de
Suzuki,Rogério Mamoru
Silva,Kellen Lagares Ferreira
Soares Júnior,Jack Wild Pereira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ferreira,Wagner de Melo
Oliveira,Sidney Pereira de
Suzuki,Rogério Mamoru
Silva,Kellen Lagares Ferreira
Soares Júnior,Jack Wild Pereira
dc.subject.por.fl_str_mv acclimatization
culture media
growth regulators
histological study
orchid
topic acclimatization
culture media
growth regulators
histological study
orchid
description Abstract Catasetum macrocarpum is an epiphytic orchid that has been subjected to strong environmental pressure in the state of Tocantins. This investigation aimed at studying the germination, growth and morpho-anatomical development of C. macrocarpum under in vitro conditions. The effects of three culture media on the in vitro germination and on the multiplication and growth of 90-day-old seedlings were studied: Murashige & Skoog (full- and half-strength), Knudson C, and Vacin & Went. The effects of different concentrations of benzyladenine (BA) and naphthaleneacetic acid (NAA) on the multiplication and growth of 120-day-old plants were evaluated. Anatomical studies were conducted on protocorms at different developmental stages. Acclimatization was also carried out. Knudson C was the best medium for seed germination whereas Vacin & Went promoted the greatest protocorm development. Half-strength Murashige & Skoog was the most effective medium for seedling multiplication and growth. The results revealed that 1 mg L-1 BA was the best treatment for shoot proliferation and leaf production. NAA at 0.5 mg L-1 strongly favored root formation. The anatomical study revealed that the early stages of C. macrocarpum development do not always coincide with the morphological phases described. The acclimatization of C. macrocarpum plants provided successful results regarding plant survival.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602018000402137
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602018000402137
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/2175-7860201869442
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Pesquisas Jardim Botânico do Rio de Janeiro
publisher.none.fl_str_mv Instituto de Pesquisas Jardim Botânico do Rio de Janeiro
dc.source.none.fl_str_mv Rodriguésia v.69 n.4 2018
reponame:Rodriguésia (Online)
instname:Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ)
instacron:JBRJ
instname_str Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ)
instacron_str JBRJ
institution JBRJ
reponame_str Rodriguésia (Online)
collection Rodriguésia (Online)
repository.name.fl_str_mv Rodriguésia (Online) - Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ)
repository.mail.fl_str_mv rodriguesia@jbrj.gov.br
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