Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Rodriguésia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602018000402137 |
Resumo: | Abstract Catasetum macrocarpum is an epiphytic orchid that has been subjected to strong environmental pressure in the state of Tocantins. This investigation aimed at studying the germination, growth and morpho-anatomical development of C. macrocarpum under in vitro conditions. The effects of three culture media on the in vitro germination and on the multiplication and growth of 90-day-old seedlings were studied: Murashige & Skoog (full- and half-strength), Knudson C, and Vacin & Went. The effects of different concentrations of benzyladenine (BA) and naphthaleneacetic acid (NAA) on the multiplication and growth of 120-day-old plants were evaluated. Anatomical studies were conducted on protocorms at different developmental stages. Acclimatization was also carried out. Knudson C was the best medium for seed germination whereas Vacin & Went promoted the greatest protocorm development. Half-strength Murashige & Skoog was the most effective medium for seedling multiplication and growth. The results revealed that 1 mg L-1 BA was the best treatment for shoot proliferation and leaf production. NAA at 0.5 mg L-1 strongly favored root formation. The anatomical study revealed that the early stages of C. macrocarpum development do not always coincide with the morphological phases described. The acclimatization of C. macrocarpum plants provided successful results regarding plant survival. |
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Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitroacclimatizationculture mediagrowth regulatorshistological studyorchidAbstract Catasetum macrocarpum is an epiphytic orchid that has been subjected to strong environmental pressure in the state of Tocantins. This investigation aimed at studying the germination, growth and morpho-anatomical development of C. macrocarpum under in vitro conditions. The effects of three culture media on the in vitro germination and on the multiplication and growth of 90-day-old seedlings were studied: Murashige & Skoog (full- and half-strength), Knudson C, and Vacin & Went. The effects of different concentrations of benzyladenine (BA) and naphthaleneacetic acid (NAA) on the multiplication and growth of 120-day-old plants were evaluated. Anatomical studies were conducted on protocorms at different developmental stages. Acclimatization was also carried out. Knudson C was the best medium for seed germination whereas Vacin & Went promoted the greatest protocorm development. Half-strength Murashige & Skoog was the most effective medium for seedling multiplication and growth. The results revealed that 1 mg L-1 BA was the best treatment for shoot proliferation and leaf production. NAA at 0.5 mg L-1 strongly favored root formation. The anatomical study revealed that the early stages of C. macrocarpum development do not always coincide with the morphological phases described. The acclimatization of C. macrocarpum plants provided successful results regarding plant survival.Instituto de Pesquisas Jardim Botânico do Rio de Janeiro2018-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602018000402137Rodriguésia v.69 n.4 2018reponame:Rodriguésia (Online)instname:Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ)instacron:JBRJ10.1590/2175-7860201869442info:eu-repo/semantics/openAccessFerreira,Wagner de MeloOliveira,Sidney Pereira deSuzuki,Rogério MamoruSilva,Kellen Lagares FerreiraSoares Júnior,Jack Wild Pereiraeng2018-12-14T00:00:00Zoai:scielo:S2175-78602018000402137Revistahttp://rodriguesia.jbrj.gov.br/https://old.scielo.br/oai/scielo-oai.phprodriguesia@jbrj.gov.br2175-78600370-6583opendoar:2018-12-14T00:00Rodriguésia (Online) - Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ)false |
dc.title.none.fl_str_mv |
Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro |
title |
Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro |
spellingShingle |
Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro Ferreira,Wagner de Melo acclimatization culture media growth regulators histological study orchid |
title_short |
Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro |
title_full |
Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro |
title_fullStr |
Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro |
title_full_unstemmed |
Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro |
title_sort |
Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro |
author |
Ferreira,Wagner de Melo |
author_facet |
Ferreira,Wagner de Melo Oliveira,Sidney Pereira de Suzuki,Rogério Mamoru Silva,Kellen Lagares Ferreira Soares Júnior,Jack Wild Pereira |
author_role |
author |
author2 |
Oliveira,Sidney Pereira de Suzuki,Rogério Mamoru Silva,Kellen Lagares Ferreira Soares Júnior,Jack Wild Pereira |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Ferreira,Wagner de Melo Oliveira,Sidney Pereira de Suzuki,Rogério Mamoru Silva,Kellen Lagares Ferreira Soares Júnior,Jack Wild Pereira |
dc.subject.por.fl_str_mv |
acclimatization culture media growth regulators histological study orchid |
topic |
acclimatization culture media growth regulators histological study orchid |
description |
Abstract Catasetum macrocarpum is an epiphytic orchid that has been subjected to strong environmental pressure in the state of Tocantins. This investigation aimed at studying the germination, growth and morpho-anatomical development of C. macrocarpum under in vitro conditions. The effects of three culture media on the in vitro germination and on the multiplication and growth of 90-day-old seedlings were studied: Murashige & Skoog (full- and half-strength), Knudson C, and Vacin & Went. The effects of different concentrations of benzyladenine (BA) and naphthaleneacetic acid (NAA) on the multiplication and growth of 120-day-old plants were evaluated. Anatomical studies were conducted on protocorms at different developmental stages. Acclimatization was also carried out. Knudson C was the best medium for seed germination whereas Vacin & Went promoted the greatest protocorm development. Half-strength Murashige & Skoog was the most effective medium for seedling multiplication and growth. The results revealed that 1 mg L-1 BA was the best treatment for shoot proliferation and leaf production. NAA at 0.5 mg L-1 strongly favored root formation. The anatomical study revealed that the early stages of C. macrocarpum development do not always coincide with the morphological phases described. The acclimatization of C. macrocarpum plants provided successful results regarding plant survival. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602018000402137 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602018000402137 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/2175-7860201869442 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Pesquisas Jardim Botânico do Rio de Janeiro |
publisher.none.fl_str_mv |
Instituto de Pesquisas Jardim Botânico do Rio de Janeiro |
dc.source.none.fl_str_mv |
Rodriguésia v.69 n.4 2018 reponame:Rodriguésia (Online) instname:Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ) instacron:JBRJ |
instname_str |
Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ) |
instacron_str |
JBRJ |
institution |
JBRJ |
reponame_str |
Rodriguésia (Online) |
collection |
Rodriguésia (Online) |
repository.name.fl_str_mv |
Rodriguésia (Online) - Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ) |
repository.mail.fl_str_mv |
rodriguesia@jbrj.gov.br |
_version_ |
1752128673550434304 |