Análise da expressão de RNA mensageiro de genes relacionados ao metabolismo e sobrevivência celular no miocárdio de camundongos machos e fêmeas adultos submetidos à exposição pré-natal ao álcool
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/3316 |
Resumo: | Exposure to alcohol during pregnancy (EPA) is a worldwide public health problem. It is believed that alcohol favors the formation of cell death-induced teratogenicity due to an imbalance between the production of reactive oxygen species and endogenous antioxidants. Few studies have evaluated the long-term impact and cellular mechanisms involved in carriers of Fetal Alcohol Spectrum Disorders (FASD). Thus, further studies are needed to assess how molecular changes are caused by EPA. The aim of this study was to analyze the expression of messenger RNA (mRNA) of genes participating in the pathways of metabolism and cell survival in the myocardium. Thus, using 10 inbred C57Bl / 6 mice exposed to 10% alcohol (v / v), they were analyzed as mRNA expressions of component genes of access pathways and cellular metabolism (AKT-I, VEGFA, GAPDH, HK1, NDUFA3, PFKM, SLC2A1, TAZ and UCP2) in the myocardium of male animals and auction with prenatal exposure to alcohol. An EPA module for the expression of all responsible genes, including GAPDH which is recognized as the endogenous control gene in molecular reactions. Between males and betrothed, there was no difference in expression between 2 genes (HK1 and TAZ), however all other differences in gene expression related to angiogenesis, metabolism and cell survival between males and betrothed with EPA. In conclusion, our data found that EPA resulted in transcriptional changes that persisted in the myocardium into adulthood, being more pronounced in males than in the second. We also observed that, separately from EPA, there seems to be greater activation of cardioprotection in relation to the oxidative stress generated by alcohol. |
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Silva Junior, Jose Antoniohttp://lattes.cnpq.br/1990427833073161Silva Junior, Jose Antoniohttp://lattes.cnpq.br/1990427833073161Dalboni, Maria Aparecidahttp://lattes.cnpq.br/9818040147487320Baltatu, Luciana Aparecida Camposhttp://lattes.cnpq.br/2861783681242132http://lattes.cnpq.br/9490809710274433Silva, Jose Almir Alves da2024-04-08T21:14:51Z2021-12-16Silva, Jose Almir Alves da. Análise da expressão de RNA mensageiro de genes relacionados ao metabolismo e sobrevivência celular no miocárdio de camundongos machos e fêmeas adultos submetidos à exposição pré-natal ao álcool. 2021. 83 f. Dissertação( Programa de Mestrado em Medicina) - Universidade Nove de Julho, São Paulo.http://bibliotecatede.uninove.br/handle/tede/3316Exposure to alcohol during pregnancy (EPA) is a worldwide public health problem. It is believed that alcohol favors the formation of cell death-induced teratogenicity due to an imbalance between the production of reactive oxygen species and endogenous antioxidants. Few studies have evaluated the long-term impact and cellular mechanisms involved in carriers of Fetal Alcohol Spectrum Disorders (FASD). Thus, further studies are needed to assess how molecular changes are caused by EPA. The aim of this study was to analyze the expression of messenger RNA (mRNA) of genes participating in the pathways of metabolism and cell survival in the myocardium. Thus, using 10 inbred C57Bl / 6 mice exposed to 10% alcohol (v / v), they were analyzed as mRNA expressions of component genes of access pathways and cellular metabolism (AKT-I, VEGFA, GAPDH, HK1, NDUFA3, PFKM, SLC2A1, TAZ and UCP2) in the myocardium of male animals and auction with prenatal exposure to alcohol. An EPA module for the expression of all responsible genes, including GAPDH which is recognized as the endogenous control gene in molecular reactions. Between males and betrothed, there was no difference in expression between 2 genes (HK1 and TAZ), however all other differences in gene expression related to angiogenesis, metabolism and cell survival between males and betrothed with EPA. In conclusion, our data found that EPA resulted in transcriptional changes that persisted in the myocardium into adulthood, being more pronounced in males than in the second. We also observed that, separately from EPA, there seems to be greater activation of cardioprotection in relation to the oxidative stress generated by alcohol.A exposição ao álcool no período gestacional (EPA) é um problema de saúde pública mundial. Acredita-se que o álcool favoreça a formação de teratogenias induzidas por morte celular em decorrência de desequilíbrio entre a produção de espécies reativas de oxigênio e antioxidantes endógenos. Poucos estudos têm avaliado o impacto e os mecanismos celulares a longo prazo envolvidos em indivíduos portadores das Desordens do Espectro Alcoólico Fetal (FASD). Desta forma, faz-se necessário novos estudos que avaliem as alterações moleculares causadas pelo EPA. O objetivo deste estudo foi analisar a expressão de RNA mensageiro (RNAm) de genes participantes das vias do metabolismo e sobrevida celular no miocárdio. Desta forma, utilizando 10 camundongos isogênicos C57Bl/6 expostos ao álcool a 10% (v/v), forma analisadas as expressões de RNAm de genes componentes de vias de sobrevivência e metabolismo celular (AKT-I, VEGFA, GAPDH, HK1, NDUFA3, PFKM, SLC2A1, TAZ e UCP2) no miocárdio de animais machos e fêmeas com exposição prénatal ao álcool. A EPA modulou a expressão de todos os genes analisados, inclusive do GAPDH que é reconhecido gene endógeno de controle em reações moleculares. Entre machos e fêmeas, não houve diferença de expressão entre 2 genes (HK1 e TAZ), entretanto todos os outros apresentaram diferenças nas expressões de genes relacionados à angiogênese, metabolismo e sobrevida celular entre machos e fêmeas com EPA. Em conclusão, nossos dados sugerem que a EPA resultou em modificações transcricionais que persistiram no miocárdio na fase adulta, sendo mais pronunciadas em machos que em fêmeas. Observamos também que, em fêmeas EPA, parece haver maior ativação de cardioproteção em relação ao estresse oxidativo gerado pelo álcoolSubmitted by Nadir Basilio (nadirsb@uninove.br) on 2024-04-08T21:14:51Z No. of bitstreams: 1 José Almir Alves da Silva.pdf: 1294329 bytes, checksum: fbbf11a102384471ba9b7648a666790e (MD5)Made available in DSpace on 2024-04-08T21:14:51Z (GMT). No. of bitstreams: 1 José Almir Alves da Silva.pdf: 1294329 bytes, checksum: fbbf11a102384471ba9b7648a666790e (MD5) Previous issue date: 2021-12-16application/pdfporUniversidade Nove de JulhoPrograma de Mestrado em MedicinaUNINOVEBrasilSaúdeálcoolexpressão gênicamiocárdiometabolismosobrevivência celularalcoholgene expressionmyocardiummetabolismcell survivalCIENCIAS DA SAUDEAnálise da expressão de RNA mensageiro de genes relacionados ao metabolismo e sobrevivência celular no miocárdio de camundongos machos e fêmeas adultos submetidos à exposição pré-natal ao álcoolAnalysis of messenger RNA expression of genes related to metabolism and cell survival in the myocardium of adult male and female mice submitted to prenatal alcohol exposureinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALJosé Almir Alves da Silva.pdfJosé Almir Alves da Silva.pdfapplication/pdf1294329http://localhost:8080/tede/bitstream/tede/3316/2/Jos%C3%A9+Almir+Alves+da+Silva.pdffbbf11a102384471ba9b7648a666790eMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/3316/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/33162024-04-08 18:14:51.062oai:localhost:tede/3316Tk9UQTogQ09MT1FVRSBBUVVJIEEgU1VBIFBSw5NQUklBIExJQ0VOw4dBCkVzdGEgbGljZW7Dp2EgZGUgZXhlbXBsbyDDqSBmb3JuZWNpZGEgYXBlbmFzIHBhcmEgZmlucyBpbmZvcm1hdGl2b3MuCgpMSUNFTsOHQSBERSBESVNUUklCVUnDh8ODTyBOw4NPLUVYQ0xVU0lWQQoKQ29tIGEgYXByZXNlbnRhw6fDo28gZGVzdGEgbGljZW7Dp2EsIHZvY8OqIChvIGF1dG9yIChlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSDDoCBVbml2ZXJzaWRhZGUgClhYWCAoU2lnbGEgZGEgVW5pdmVyc2lkYWRlKSBvIGRpcmVpdG8gbsOjby1leGNsdXNpdm8gZGUgcmVwcm9kdXppciwgIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IApkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0csO0bmljbyBlIAplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBwb2RlLCBzZW0gYWx0ZXJhciBvIGNvbnRlw7pkbywgdHJhbnNwb3IgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIApwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgU2lnbGEgZGUgVW5pdmVyc2lkYWRlIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPDs3BpYSBhIHN1YSB0ZXNlIG91IApkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcyAKbmVzdGEgbGljZW7Dp2EuIFZvY8OqIHRhbWLDqW0gZGVjbGFyYSBxdWUgbyBkZXDDs3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBuw6NvLCBxdWUgc2VqYSBkZSBzZXUgCmNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3XDqW0uCgpDYXNvIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiAKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSAKb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIAppZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250ZcO6ZG8gZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFRFU0UgT1UgRElTU0VSVEHDh8ODTyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0PDjU5JTyBPVSAKQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBTSUdMQSBERSAKVU5JVkVSU0lEQURFLCBWT0PDiiBERUNMQVJBIFFVRSBSRVNQRUlUT1UgVE9ET1MgRSBRVUFJU1FVRVIgRElSRUlUT1MgREUgUkVWSVPDg08gQ09NTyAKVEFNQsOJTSBBUyBERU1BSVMgT0JSSUdBw4fDlUVTIEVYSUdJREFTIFBPUiBDT05UUkFUTyBPVSBBQ09SRE8uCgpBIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIChzKSBvdSBvKHMpIG5vbWUocykgZG8ocykgCmRldGVudG9yKGVzKSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzIApjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgo=Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2024-04-08T21:14:51Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Análise da expressão de RNA mensageiro de genes relacionados ao metabolismo e sobrevivência celular no miocárdio de camundongos machos e fêmeas adultos submetidos à exposição pré-natal ao álcool |
dc.title.alternative.eng.fl_str_mv |
Analysis of messenger RNA expression of genes related to metabolism and cell survival in the myocardium of adult male and female mice submitted to prenatal alcohol exposure |
title |
Análise da expressão de RNA mensageiro de genes relacionados ao metabolismo e sobrevivência celular no miocárdio de camundongos machos e fêmeas adultos submetidos à exposição pré-natal ao álcool |
spellingShingle |
Análise da expressão de RNA mensageiro de genes relacionados ao metabolismo e sobrevivência celular no miocárdio de camundongos machos e fêmeas adultos submetidos à exposição pré-natal ao álcool Silva, Jose Almir Alves da álcool expressão gênica miocárdio metabolismo sobrevivência celular alcohol gene expression myocardium metabolism cell survival CIENCIAS DA SAUDE |
title_short |
Análise da expressão de RNA mensageiro de genes relacionados ao metabolismo e sobrevivência celular no miocárdio de camundongos machos e fêmeas adultos submetidos à exposição pré-natal ao álcool |
title_full |
Análise da expressão de RNA mensageiro de genes relacionados ao metabolismo e sobrevivência celular no miocárdio de camundongos machos e fêmeas adultos submetidos à exposição pré-natal ao álcool |
title_fullStr |
Análise da expressão de RNA mensageiro de genes relacionados ao metabolismo e sobrevivência celular no miocárdio de camundongos machos e fêmeas adultos submetidos à exposição pré-natal ao álcool |
title_full_unstemmed |
Análise da expressão de RNA mensageiro de genes relacionados ao metabolismo e sobrevivência celular no miocárdio de camundongos machos e fêmeas adultos submetidos à exposição pré-natal ao álcool |
title_sort |
Análise da expressão de RNA mensageiro de genes relacionados ao metabolismo e sobrevivência celular no miocárdio de camundongos machos e fêmeas adultos submetidos à exposição pré-natal ao álcool |
author |
Silva, Jose Almir Alves da |
author_facet |
Silva, Jose Almir Alves da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva Junior, Jose Antonio |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1990427833073161 |
dc.contributor.referee1.fl_str_mv |
Silva Junior, Jose Antonio |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1990427833073161 |
dc.contributor.referee2.fl_str_mv |
Dalboni, Maria Aparecida |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9818040147487320 |
dc.contributor.referee3.fl_str_mv |
Baltatu, Luciana Aparecida Campos |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/2861783681242132 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9490809710274433 |
dc.contributor.author.fl_str_mv |
Silva, Jose Almir Alves da |
contributor_str_mv |
Silva Junior, Jose Antonio Silva Junior, Jose Antonio Dalboni, Maria Aparecida Baltatu, Luciana Aparecida Campos |
dc.subject.por.fl_str_mv |
álcool expressão gênica miocárdio metabolismo sobrevivência celular |
topic |
álcool expressão gênica miocárdio metabolismo sobrevivência celular alcohol gene expression myocardium metabolism cell survival CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
alcohol gene expression myocardium metabolism cell survival |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Exposure to alcohol during pregnancy (EPA) is a worldwide public health problem. It is believed that alcohol favors the formation of cell death-induced teratogenicity due to an imbalance between the production of reactive oxygen species and endogenous antioxidants. Few studies have evaluated the long-term impact and cellular mechanisms involved in carriers of Fetal Alcohol Spectrum Disorders (FASD). Thus, further studies are needed to assess how molecular changes are caused by EPA. The aim of this study was to analyze the expression of messenger RNA (mRNA) of genes participating in the pathways of metabolism and cell survival in the myocardium. Thus, using 10 inbred C57Bl / 6 mice exposed to 10% alcohol (v / v), they were analyzed as mRNA expressions of component genes of access pathways and cellular metabolism (AKT-I, VEGFA, GAPDH, HK1, NDUFA3, PFKM, SLC2A1, TAZ and UCP2) in the myocardium of male animals and auction with prenatal exposure to alcohol. An EPA module for the expression of all responsible genes, including GAPDH which is recognized as the endogenous control gene in molecular reactions. Between males and betrothed, there was no difference in expression between 2 genes (HK1 and TAZ), however all other differences in gene expression related to angiogenesis, metabolism and cell survival between males and betrothed with EPA. In conclusion, our data found that EPA resulted in transcriptional changes that persisted in the myocardium into adulthood, being more pronounced in males than in the second. We also observed that, separately from EPA, there seems to be greater activation of cardioprotection in relation to the oxidative stress generated by alcohol. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021-12-16 |
dc.date.accessioned.fl_str_mv |
2024-04-08T21:14:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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dc.identifier.citation.fl_str_mv |
Silva, Jose Almir Alves da. Análise da expressão de RNA mensageiro de genes relacionados ao metabolismo e sobrevivência celular no miocárdio de camundongos machos e fêmeas adultos submetidos à exposição pré-natal ao álcool. 2021. 83 f. Dissertação( Programa de Mestrado em Medicina) - Universidade Nove de Julho, São Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/3316 |
identifier_str_mv |
Silva, Jose Almir Alves da. Análise da expressão de RNA mensageiro de genes relacionados ao metabolismo e sobrevivência celular no miocárdio de camundongos machos e fêmeas adultos submetidos à exposição pré-natal ao álcool. 2021. 83 f. Dissertação( Programa de Mestrado em Medicina) - Universidade Nove de Julho, São Paulo. |
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http://bibliotecatede.uninove.br/handle/tede/3316 |
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