Efeito do laser de baixa pot??ncia sobre c??lulas musculares C2C12 submetidas ?? les??o por veneno da serpente Bothrops jararacussu.
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/tede/handle/tede/875 |
Resumo: | The venom of snakes of the genus Bothrops, induces an intense inflammatory reaction that leads to develop local tissue necrosis. The serum therapy is efficacious in neutralizing the systemic effects, but its action does not extend the local manifestations. The low power laser (LPB) is used in situations of muscles damage, due to its biological effects, such as analgesics, anti- inflammatory and healing. Thus, the objective of this study was to analyse the effect of LPB in C2C12 muscle cells subjected to injury by Bothrops jararacussu venom by: cell viability, monolayer integrity and release of CK and LDH enzymes. The cells received the venom (12.5 ??l/mL) and were irradiated immediately with LBP Aluminum Gallium Indium phosphorus and Aluminium gallium arsenide, wavelength (??) 685 nm and 830 nm, energy density of 4Jcm2, potency of 100 mW, total energy 1.3 J, exposure time of 13 sec point and the cells were incubated for 15, 30 and 60 minutes. The results showed that the venom affected the viability and integrity of muscle cells in a dose-dependent manner, being chosen the dose of 12.5 ??l/mL for the realization of experiments with the LBP. The LBP caused a significantly increased in cell viability at all times analyzed at ?? 830 nm, however the LBP in the ?? at 685 nm was effective only at 15 and 30 min. The LBP decreased the release of CK and LDH in all time analyzed and with two wavelengths used. However, the LBP was unable to protect cells against cell detachment caused by venom. Thus, this study has shown that the LBP was able to protect the C2C12 muscle cells against the myotoxic effect of B. jararacussu venom and this protection is related to the protective effect of cell membrane. Furthermore, the results obtained suggest that the LBP can be considered a therapeutic tool in patients bitten by bothropic snakes. |
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Ferrari, Raquel Agnelli Mesquitahttp://lattes.cnpq.br/7048141683554943Dale, Camila Squarzonihttp://lattes.cnpq.br/7054669706344704Fernandes, Kristiane Santos PortaCPF:36403488856http://lattes.cnpq.br/2228147425389643Silva, Camila Aparecida Alves da2015-04-22T17:20:39Z2014-04-162012-12-13SILVA, Camila Aparecida Alves da. Efeito do laser de baixa pot??ncia sobre c??lulas musculares c2c12 submetidas ?? les??o por veneno da serpente Bothrops jararacussu.. 2012. 79 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2012.http://bibliotecatede.uninove.br/tede/handle/tede/875The venom of snakes of the genus Bothrops, induces an intense inflammatory reaction that leads to develop local tissue necrosis. The serum therapy is efficacious in neutralizing the systemic effects, but its action does not extend the local manifestations. The low power laser (LPB) is used in situations of muscles damage, due to its biological effects, such as analgesics, anti- inflammatory and healing. Thus, the objective of this study was to analyse the effect of LPB in C2C12 muscle cells subjected to injury by Bothrops jararacussu venom by: cell viability, monolayer integrity and release of CK and LDH enzymes. The cells received the venom (12.5 ??l/mL) and were irradiated immediately with LBP Aluminum Gallium Indium phosphorus and Aluminium gallium arsenide, wavelength (??) 685 nm and 830 nm, energy density of 4Jcm2, potency of 100 mW, total energy 1.3 J, exposure time of 13 sec point and the cells were incubated for 15, 30 and 60 minutes. The results showed that the venom affected the viability and integrity of muscle cells in a dose-dependent manner, being chosen the dose of 12.5 ??l/mL for the realization of experiments with the LBP. The LBP caused a significantly increased in cell viability at all times analyzed at ?? 830 nm, however the LBP in the ?? at 685 nm was effective only at 15 and 30 min. The LBP decreased the release of CK and LDH in all time analyzed and with two wavelengths used. However, the LBP was unable to protect cells against cell detachment caused by venom. Thus, this study has shown that the LBP was able to protect the C2C12 muscle cells against the myotoxic effect of B. jararacussu venom and this protection is related to the protective effect of cell membrane. Furthermore, the results obtained suggest that the LBP can be considered a therapeutic tool in patients bitten by bothropic snakes.O veneno das serpentes do g??nero Bothrops, induz uma intensa rea????o inflamat??ria local podendo evoluir para necrose tecidual. A soroterapia apresenta efic??cia em neutralizar os efeitos sist??micos, por??m sua a????o n??o se estende as manifesta????es locais. O laser de baixa pot??ncia (LBP) ?? usado em situa????es de les??o muscular, pois apresenta efeitos biol??gicos, tais como analg??sicos, antiinflamat??rios e cicatrizantes. O objetivo deste trabalho foi analisar o efeito do LBP em c??lulas musculares C2C12 submetidas ?? les??o por veneno da serpente Bothrops jararacussu quanto a: viabilidade, descolamento celular e libera????o das enzimas CK e LDH. As c??lulas receberam o veneno (12.5 ??g/mL) e foram imediatamente irradiadas com LBP ??ndio G??lio Alum??nio F??sforo e Arseneto de G??lio Alum??nio, nos comprimentos de onda (??) 685nm e 830nm, densidade de energia 4J/cm2, pot??ncia de 100mW, energia total 1.3J, tempo de aplica????o de 13 segundos por ponto e as c??lulas foram incubadas por 15, 30 e 60 minutos.Os resultados demonstraram que o veneno afetou a viabilidade e a integridade das c??lulas de forma dose-dependente, sendo escolhida a concentra????o 12.5 ??g/mL para a realiza????o dos experimentos com o LBP. O LBP causou aumento significativo na viabilidade celular, nos tempos analisados no ?? 830 nm, entretanto o LBP no ?? 685 nm foi efetivo somente nos tempos de 15 e 30 min. O LBP diminuiu a libera????o de CK e LDH em todos os tempos analisados e com os dois ?? utilizados. No entanto, o LBP n??o foi capaz de proteger as c??lulas contra o descolamento celular causado pelo veneno. Desta forma, verificou-se que o LBP foi capaz de proteger as c??lulas musculares C2C12 contra o potente efeito miot??xico do veneno B. jararacussu e que esta prote????o est?? relacionada ao efeito protetor da membrana celular. Ainda, os resultados obtidos sugerem que o LBP pode ser considerado uma ferramenta terap??utica eficaz em pacientes picados por serpentes.Made available in DSpace on 2015-04-22T17:20:39Z (GMT). No. of bitstreams: 1 B_Camila Aparecida Alves da Silva.pdf: 1172530 bytes, checksum: e2b81c241e8b50eba3f684932dfbdf5c (MD5) Previous issue date: 2012-12-13application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBRSa??dec??lulas muscularesmionecroselaserviabilidade e diferencia????o celularmuscle cellsmyonecrosislaserviabilityand cell differentiationCIENCIAS DA SAUDEEfeito do laser de baixa pot??ncia sobre c??lulas musculares C2C12 submetidas ?? les??o por veneno da serpente Bothrops jararacussu.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALB_Camila Aparecida Alves da Silva.pdfapplication/pdf1172530http://localhost:8080/tede/bitstream/tede/875/1/B_Camila+Aparecida+Alves+da+Silva.pdfe2b81c241e8b50eba3f684932dfbdf5cMD51tede/8752018-06-15 12:41:52.546oai:localhost:tede/875Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-15T15:41:52Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito do laser de baixa pot??ncia sobre c??lulas musculares C2C12 submetidas ?? les??o por veneno da serpente Bothrops jararacussu. |
title |
Efeito do laser de baixa pot??ncia sobre c??lulas musculares C2C12 submetidas ?? les??o por veneno da serpente Bothrops jararacussu. |
spellingShingle |
Efeito do laser de baixa pot??ncia sobre c??lulas musculares C2C12 submetidas ?? les??o por veneno da serpente Bothrops jararacussu. Silva, Camila Aparecida Alves da c??lulas musculares mionecrose laser viabilidade e diferencia????o celular muscle cells myonecrosis laser viability and cell differentiation CIENCIAS DA SAUDE |
title_short |
Efeito do laser de baixa pot??ncia sobre c??lulas musculares C2C12 submetidas ?? les??o por veneno da serpente Bothrops jararacussu. |
title_full |
Efeito do laser de baixa pot??ncia sobre c??lulas musculares C2C12 submetidas ?? les??o por veneno da serpente Bothrops jararacussu. |
title_fullStr |
Efeito do laser de baixa pot??ncia sobre c??lulas musculares C2C12 submetidas ?? les??o por veneno da serpente Bothrops jararacussu. |
title_full_unstemmed |
Efeito do laser de baixa pot??ncia sobre c??lulas musculares C2C12 submetidas ?? les??o por veneno da serpente Bothrops jararacussu. |
title_sort |
Efeito do laser de baixa pot??ncia sobre c??lulas musculares C2C12 submetidas ?? les??o por veneno da serpente Bothrops jararacussu. |
author |
Silva, Camila Aparecida Alves da |
author_facet |
Silva, Camila Aparecida Alves da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Ferrari, Raquel Agnelli Mesquita |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7048141683554943 |
dc.contributor.referee1.fl_str_mv |
Dale, Camila Squarzoni |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7054669706344704 |
dc.contributor.referee2.fl_str_mv |
Fernandes, Kristiane Santos Porta |
dc.contributor.authorID.fl_str_mv |
CPF:36403488856 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2228147425389643 |
dc.contributor.author.fl_str_mv |
Silva, Camila Aparecida Alves da |
contributor_str_mv |
Ferrari, Raquel Agnelli Mesquita Dale, Camila Squarzoni Fernandes, Kristiane Santos Porta |
dc.subject.por.fl_str_mv |
c??lulas musculares mionecrose laser viabilidade e diferencia????o celular |
topic |
c??lulas musculares mionecrose laser viabilidade e diferencia????o celular muscle cells myonecrosis laser viability and cell differentiation CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
muscle cells myonecrosis laser viability and cell differentiation |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
The venom of snakes of the genus Bothrops, induces an intense inflammatory reaction that leads to develop local tissue necrosis. The serum therapy is efficacious in neutralizing the systemic effects, but its action does not extend the local manifestations. The low power laser (LPB) is used in situations of muscles damage, due to its biological effects, such as analgesics, anti- inflammatory and healing. Thus, the objective of this study was to analyse the effect of LPB in C2C12 muscle cells subjected to injury by Bothrops jararacussu venom by: cell viability, monolayer integrity and release of CK and LDH enzymes. The cells received the venom (12.5 ??l/mL) and were irradiated immediately with LBP Aluminum Gallium Indium phosphorus and Aluminium gallium arsenide, wavelength (??) 685 nm and 830 nm, energy density of 4Jcm2, potency of 100 mW, total energy 1.3 J, exposure time of 13 sec point and the cells were incubated for 15, 30 and 60 minutes. The results showed that the venom affected the viability and integrity of muscle cells in a dose-dependent manner, being chosen the dose of 12.5 ??l/mL for the realization of experiments with the LBP. The LBP caused a significantly increased in cell viability at all times analyzed at ?? 830 nm, however the LBP in the ?? at 685 nm was effective only at 15 and 30 min. The LBP decreased the release of CK and LDH in all time analyzed and with two wavelengths used. However, the LBP was unable to protect cells against cell detachment caused by venom. Thus, this study has shown that the LBP was able to protect the C2C12 muscle cells against the myotoxic effect of B. jararacussu venom and this protection is related to the protective effect of cell membrane. Furthermore, the results obtained suggest that the LBP can be considered a therapeutic tool in patients bitten by bothropic snakes. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-12-13 |
dc.date.available.fl_str_mv |
2014-04-16 |
dc.date.accessioned.fl_str_mv |
2015-04-22T17:20:39Z |
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info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Camila Aparecida Alves da. Efeito do laser de baixa pot??ncia sobre c??lulas musculares c2c12 submetidas ?? les??o por veneno da serpente Bothrops jararacussu.. 2012. 79 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2012. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/tede/handle/tede/875 |
identifier_str_mv |
SILVA, Camila Aparecida Alves da. Efeito do laser de baixa pot??ncia sobre c??lulas musculares c2c12 submetidas ?? les??o por veneno da serpente Bothrops jararacussu.. 2012. 79 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2012. |
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http://bibliotecatede.uninove.br/tede/handle/tede/875 |
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UNINOVE |
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