Efeitos do treinamento f??sico aer??bio na inflama????o pulmonar al??rgica cr??nica em camundongos: papel da sinaliza????o purin??rgica

Detalhes bibliográficos
Autor(a) principal: Greiffo, Fl??via Regina
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da Uninove
Texto Completo: http://bibliotecatede.uninove.br/handle/tede/1336
Resumo: In the last years, a growing number of studies have demonstrated that the aerobic physical training (APT), performed in the proper intensity, duration and frequency, display several benefits to asthmatic patients, including improvement in quality of life, reduction in the levels of anxiety and depression, improvements in minute ventilation, reduction in the number of crisis, reduction in the levels of exhaled nitric oxide, beyond reduced daily needing of corticosteroids. More recently, was demonstrated for the first time that APT presents a direct anti-inflammatory effect on the airways of asthmatic patient, since that APT reduced the number of eosinophils in the induced sputum of these patients. However, several questions and hypothesis were raised up about the possible mechanism involved in these anti-inflammatory effects of APT for asthma. In this way, a growing number of experimental studies using models of asthma have been published, revealing possible mechanism involved of action APT for asthma. On the other hand, until this moment no study evaluated the effects of APT on the via of purinergic receptors, a family of receptors that present a central role in the physiopathology of asthma. Therefore, the present study aim is to evaluate if the anti-inflammatory effects of APT for asthma is mediate, at least partially, by the inhibition of expression of purinergic receptor P2X7, as well as the extracellular adenosine triphosphate (ATP). Thus, using 40 C57Bl/6 mice, divided in 4 experimental groups (Control, n = 10), (Exercise, n = 10), (Asthma, n = 10) and (Asthma + Exercise, n = 10). The experimental model of asthma (chronic pulmonary allergic inflammation) was induced through the intra-peritoneal injection of ovalbumin solution on days 0, 14, 21 and 42 followed by inhalation of 1%, 3% and 5% ovalbumin, 3x/week, and beginning on 21st day until the 53rd day of experimental protocol. To evaluate the therapeutic effects of APT on the inflammation and on the via of purinergic receptors, the low intensity APT was performed 5x/week, 60 min/session, begining on 27th day of experimental protocol until the 53rd day, when the airways inflammation is already established. Twenty-four hours after the last inhalation and training session, mice were anesthetized, tracheotomized, canuled and the bronchoalveolar lavage was collected and analyzed for the ATP levels and also the total number of cells as well as the differential number of cells. The analysis of hyperresponsiveness was performed. The histological analysis was performed to evaluate and quantify the number of lymphocytes and eosinophils in the airways wall, and also the airway remodeling. The expression of purinergic receptor P2X7 in the lung tissue homogenate was performed using western blotting and also the immunohistochemistry technique.
id NOVE_399ad0780430916c33c77cf084c6299d
oai_identifier_str oai:localhost:tede/1336
network_acronym_str NOVE
network_name_str Biblioteca Digital de Teses e Dissertações da Uninove
repository_id_str
spelling Vieira, Rodolfo de Paulahttp://lattes.cnpq.br/9213556008468472Vieira, Rodolfo de Paulahttp://lattes.cnpq.br/9213556008468472Carvalho, Fl??vio Aimbire Soares dehttp://lattes.cnpq.br/8216583445045638Oliveira, Lu??s Vicente Franco dehttp://lattes.cnpq.br/3644494034569111http://lattes.cnpq.br/9413876266240855Greiffo, Fl??via Regina2016-05-24T21:28:26Z2015-03-02Greiffo, Fl??via Regina. Efeitos do treinamento f??sico aer??bio na inflama????o pulmonar al??rgica cr??nica em camundongos: papel da sinaliza????o purin??rgica. 2015. 61 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1336In the last years, a growing number of studies have demonstrated that the aerobic physical training (APT), performed in the proper intensity, duration and frequency, display several benefits to asthmatic patients, including improvement in quality of life, reduction in the levels of anxiety and depression, improvements in minute ventilation, reduction in the number of crisis, reduction in the levels of exhaled nitric oxide, beyond reduced daily needing of corticosteroids. More recently, was demonstrated for the first time that APT presents a direct anti-inflammatory effect on the airways of asthmatic patient, since that APT reduced the number of eosinophils in the induced sputum of these patients. However, several questions and hypothesis were raised up about the possible mechanism involved in these anti-inflammatory effects of APT for asthma. In this way, a growing number of experimental studies using models of asthma have been published, revealing possible mechanism involved of action APT for asthma. On the other hand, until this moment no study evaluated the effects of APT on the via of purinergic receptors, a family of receptors that present a central role in the physiopathology of asthma. Therefore, the present study aim is to evaluate if the anti-inflammatory effects of APT for asthma is mediate, at least partially, by the inhibition of expression of purinergic receptor P2X7, as well as the extracellular adenosine triphosphate (ATP). Thus, using 40 C57Bl/6 mice, divided in 4 experimental groups (Control, n = 10), (Exercise, n = 10), (Asthma, n = 10) and (Asthma + Exercise, n = 10). The experimental model of asthma (chronic pulmonary allergic inflammation) was induced through the intra-peritoneal injection of ovalbumin solution on days 0, 14, 21 and 42 followed by inhalation of 1%, 3% and 5% ovalbumin, 3x/week, and beginning on 21st day until the 53rd day of experimental protocol. To evaluate the therapeutic effects of APT on the inflammation and on the via of purinergic receptors, the low intensity APT was performed 5x/week, 60 min/session, begining on 27th day of experimental protocol until the 53rd day, when the airways inflammation is already established. Twenty-four hours after the last inhalation and training session, mice were anesthetized, tracheotomized, canuled and the bronchoalveolar lavage was collected and analyzed for the ATP levels and also the total number of cells as well as the differential number of cells. The analysis of hyperresponsiveness was performed. The histological analysis was performed to evaluate and quantify the number of lymphocytes and eosinophils in the airways wall, and also the airway remodeling. The expression of purinergic receptor P2X7 in the lung tissue homogenate was performed using western blotting and also the immunohistochemistry technique.Nos ??ltimos anos um crescente n??mero de estudos tem demonstrado que o treinamento f??sico aer??bio (TFA), quando realizado de maneira adequada em termos de intensidade, dura????o e freq????ncia, apresenta in??meros benef??cios para o paciente asm??tico, incluindo melhora na qualidade de vida, diminui????o dos n??veis de ansiedade e depress??o, melhora da ventila????o minuto, diminui????o do n??mero de crises, diminui????o dos n??veis de ??xido n??trico exalado, al??m de diminuir a necessidade di??ria de corticoester??ides. Recentemente, foi demonstrado pela primeira vez que o TFA apresenta um efeito antiinflamat??rio direto sobre as vias a??reas do paciente asm??tico, uma vez que o TFA diminuiu o n??mero de eosin??filos no escarro induzido desses pacientes. Entretanto, in??meras perguntas e hip??teses foram levantadas sobre os poss??veis mecanismos envolvidos nesse efeito antiinflamat??rio do TFA na asma. Nesse sentido, um crescente n??mero de estudos com animais de experimenta????o em modelos de asma tem sido publicado, os quais t??m revelado poss??veis vias de atua????o do TFA na asma. Entretanto, at?? o momento n??o existem publica????es sobre os efeitos do TFA sobre a via dos receptores purin??rgicos, os quais t??m um papel central na fisiopatologia da asma. Portanto, o presente estudo teve com objetivo avaliar se os efeitos antiinflamat??rios do TFA na asma ?? mediado, pelo menos em parte, pela inibi????o da express??o dos receptores purin??rgicos, assim como pela diminui????o dos n??veis do principal ativador dos receptores purin??rgicos, a adenosina trifosfato (ATP) extracelular. Para isso, foram utilizados 40 camundongos C57Bl/6 divididos em 4 grupos experimentais (n=10 / cada) Controle, Exerc??cio, Asma e Asma + Exerc??cio. O modelo de asma (inflama????o pulmonar al??rgica cr??nica) foi induzido por meio de inje????o intra-peritoneal com ovalbumina nos dias 0, 14, 28 e 42 seguidos por inala????o com solu????o de ovalbumina (1, 3 e 5%) tr??s vezes por semana, a partir do dia 21 at?? o dia 53 do protocolo experimental. Com o intuito de avaliar o efeito terap??utico do TFA sobre a inflama????o e sobre a via dos receptores purin??rgicos, o TFA de baixa intensidade foi realizado 5x/semana, 60 minutos/sess??o, iniciando no dia 27 do protocolo experimental at?? o dia 53, quando a inflama????o das vias a??reas j?? estava estabelecida. Vinte e quatro horas ap??s a ??ltima inala????o e sess??o de treinamento, os animais foram anestesiados, traqueostomizados, canulados e o lavado broncoalveolar foi coletado e analisado para os n??veis de ATP e tamb??m para o n??mero de c??lulas totais e contagem diferencial. A avalia????o da express??o do receptor purin??rgico P2X7 foi realizada no homogenato do tecido pulmonar por meio da t??cnica de western blotting, e tamb??m nos cortes histol??gicos por meio da t??cnica de imunohistoqu??mica. Atrav??s da t??cnica de histologia foi quantificado o n??mero de linf??citos e eosin??filos nas vias a??reas, assim como o remodelamento br??nquico. A hiperresponsividade br??nquica das vias a??reas foi avaliada atrav??s do sistema Buxco. Os resultados obtidos neste trabalho corroboram com a literatura, uma vez que o TFA reduz a inflama????o cr??nica das vias a??reas. Nossos resultados tamb??m sugerem que o TFA atua na via dos receptores purin??gicos diminuindo a inflama????o pulmonar.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2016-05-24T21:28:26Z No. of bitstreams: 1 Flavia Regina Greiffo.pdf: 886904 bytes, checksum: 4aa27a44bc94c9d06427df1b649e9b9e (MD5)Made available in DSpace on 2016-05-24T21:28:26Z (GMT). No. of bitstreams: 1 Flavia Regina Greiffo.pdf: 886904 bytes, checksum: 4aa27a44bc94c9d06427df1b649e9b9e (MD5) Previous issue date: 2015-03-02application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBrasilSa??deasmaexerc??ciodoen??as respirat??rias/ reabilita????oalergia e imunologiapulm??oinflama????oATPreceptores purin??rgicoscamundongosmiceasthmaexerciserespiratory diseases/ rehabilitationallergy and immunollogylunginflammationATPpurinergic receptorsCIENCIAS DA SAUDEEfeitos do treinamento f??sico aer??bio na inflama????o pulmonar al??rgica cr??nica em camundongos: papel da sinaliza????o purin??rgicaEffects of aerobic physical training on the chronic allergic pulmonary inflammation in mice: role of purinergic receptorsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALFlavia Regina Greiffo.pdfFlavia Regina Greiffo.pdfapplication/pdf886904http://localhost:8080/tede/bitstream/tede/1336/2/Flavia+Regina+Greiffo.pdf4aa27a44bc94c9d06427df1b649e9b9eMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/1336/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/13362016-05-24 18:28:26.378oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2016-05-24T21:28:26Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false
dc.title.por.fl_str_mv Efeitos do treinamento f??sico aer??bio na inflama????o pulmonar al??rgica cr??nica em camundongos: papel da sinaliza????o purin??rgica
dc.title.alternative.eng.fl_str_mv Effects of aerobic physical training on the chronic allergic pulmonary inflammation in mice: role of purinergic receptors
title Efeitos do treinamento f??sico aer??bio na inflama????o pulmonar al??rgica cr??nica em camundongos: papel da sinaliza????o purin??rgica
spellingShingle Efeitos do treinamento f??sico aer??bio na inflama????o pulmonar al??rgica cr??nica em camundongos: papel da sinaliza????o purin??rgica
Greiffo, Fl??via Regina
asma
exerc??cio
doen??as respirat??rias/ reabilita????o
alergia e imunologia
pulm??o
inflama????o
ATP
receptores purin??rgicos
camundongos
mice
asthma
exercise
respiratory diseases/ rehabilitation
allergy and immunollogy
lung
inflammation
ATP
purinergic receptors
CIENCIAS DA SAUDE
title_short Efeitos do treinamento f??sico aer??bio na inflama????o pulmonar al??rgica cr??nica em camundongos: papel da sinaliza????o purin??rgica
title_full Efeitos do treinamento f??sico aer??bio na inflama????o pulmonar al??rgica cr??nica em camundongos: papel da sinaliza????o purin??rgica
title_fullStr Efeitos do treinamento f??sico aer??bio na inflama????o pulmonar al??rgica cr??nica em camundongos: papel da sinaliza????o purin??rgica
title_full_unstemmed Efeitos do treinamento f??sico aer??bio na inflama????o pulmonar al??rgica cr??nica em camundongos: papel da sinaliza????o purin??rgica
title_sort Efeitos do treinamento f??sico aer??bio na inflama????o pulmonar al??rgica cr??nica em camundongos: papel da sinaliza????o purin??rgica
author Greiffo, Fl??via Regina
author_facet Greiffo, Fl??via Regina
author_role author
dc.contributor.advisor1.fl_str_mv Vieira, Rodolfo de Paula
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9213556008468472
dc.contributor.referee1.fl_str_mv Vieira, Rodolfo de Paula
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/9213556008468472
dc.contributor.referee2.fl_str_mv Carvalho, Fl??vio Aimbire Soares de
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/8216583445045638
dc.contributor.referee3.fl_str_mv Oliveira, Lu??s Vicente Franco de
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/3644494034569111
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9413876266240855
dc.contributor.author.fl_str_mv Greiffo, Fl??via Regina
contributor_str_mv Vieira, Rodolfo de Paula
Vieira, Rodolfo de Paula
Carvalho, Fl??vio Aimbire Soares de
Oliveira, Lu??s Vicente Franco de
dc.subject.por.fl_str_mv asma
exerc??cio
doen??as respirat??rias/ reabilita????o
alergia e imunologia
pulm??o
inflama????o
ATP
receptores purin??rgicos
camundongos
mice
topic asma
exerc??cio
doen??as respirat??rias/ reabilita????o
alergia e imunologia
pulm??o
inflama????o
ATP
receptores purin??rgicos
camundongos
mice
asthma
exercise
respiratory diseases/ rehabilitation
allergy and immunollogy
lung
inflammation
ATP
purinergic receptors
CIENCIAS DA SAUDE
dc.subject.eng.fl_str_mv asthma
exercise
respiratory diseases/ rehabilitation
allergy and immunollogy
lung
inflammation
ATP
purinergic receptors
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE
description In the last years, a growing number of studies have demonstrated that the aerobic physical training (APT), performed in the proper intensity, duration and frequency, display several benefits to asthmatic patients, including improvement in quality of life, reduction in the levels of anxiety and depression, improvements in minute ventilation, reduction in the number of crisis, reduction in the levels of exhaled nitric oxide, beyond reduced daily needing of corticosteroids. More recently, was demonstrated for the first time that APT presents a direct anti-inflammatory effect on the airways of asthmatic patient, since that APT reduced the number of eosinophils in the induced sputum of these patients. However, several questions and hypothesis were raised up about the possible mechanism involved in these anti-inflammatory effects of APT for asthma. In this way, a growing number of experimental studies using models of asthma have been published, revealing possible mechanism involved of action APT for asthma. On the other hand, until this moment no study evaluated the effects of APT on the via of purinergic receptors, a family of receptors that present a central role in the physiopathology of asthma. Therefore, the present study aim is to evaluate if the anti-inflammatory effects of APT for asthma is mediate, at least partially, by the inhibition of expression of purinergic receptor P2X7, as well as the extracellular adenosine triphosphate (ATP). Thus, using 40 C57Bl/6 mice, divided in 4 experimental groups (Control, n = 10), (Exercise, n = 10), (Asthma, n = 10) and (Asthma + Exercise, n = 10). The experimental model of asthma (chronic pulmonary allergic inflammation) was induced through the intra-peritoneal injection of ovalbumin solution on days 0, 14, 21 and 42 followed by inhalation of 1%, 3% and 5% ovalbumin, 3x/week, and beginning on 21st day until the 53rd day of experimental protocol. To evaluate the therapeutic effects of APT on the inflammation and on the via of purinergic receptors, the low intensity APT was performed 5x/week, 60 min/session, begining on 27th day of experimental protocol until the 53rd day, when the airways inflammation is already established. Twenty-four hours after the last inhalation and training session, mice were anesthetized, tracheotomized, canuled and the bronchoalveolar lavage was collected and analyzed for the ATP levels and also the total number of cells as well as the differential number of cells. The analysis of hyperresponsiveness was performed. The histological analysis was performed to evaluate and quantify the number of lymphocytes and eosinophils in the airways wall, and also the airway remodeling. The expression of purinergic receptor P2X7 in the lung tissue homogenate was performed using western blotting and also the immunohistochemistry technique.
publishDate 2015
dc.date.issued.fl_str_mv 2015-03-02
dc.date.accessioned.fl_str_mv 2016-05-24T21:28:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv Greiffo, Fl??via Regina. Efeitos do treinamento f??sico aer??bio na inflama????o pulmonar al??rgica cr??nica em camundongos: papel da sinaliza????o purin??rgica. 2015. 61 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.
dc.identifier.uri.fl_str_mv http://bibliotecatede.uninove.br/handle/tede/1336
identifier_str_mv Greiffo, Fl??via Regina. Efeitos do treinamento f??sico aer??bio na inflama????o pulmonar al??rgica cr??nica em camundongos: papel da sinaliza????o purin??rgica. 2015. 61 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.
url http://bibliotecatede.uninove.br/handle/tede/1336
dc.language.iso.fl_str_mv por
language por
dc.relation.cnpq.fl_str_mv 8765449414823306929
dc.relation.confidence.fl_str_mv 600
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Nove de Julho
dc.publisher.program.fl_str_mv Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o
dc.publisher.initials.fl_str_mv UNINOVE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Sa??de
publisher.none.fl_str_mv Universidade Nove de Julho
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da Uninove
instname:Universidade Nove de Julho (UNINOVE)
instacron:UNINOVE
instname_str Universidade Nove de Julho (UNINOVE)
instacron_str UNINOVE
institution UNINOVE
reponame_str Biblioteca Digital de Teses e Dissertações da Uninove
collection Biblioteca Digital de Teses e Dissertações da Uninove
bitstream.url.fl_str_mv http://localhost:8080/tede/bitstream/tede/1336/2/Flavia+Regina+Greiffo.pdf
http://localhost:8080/tede/bitstream/tede/1336/1/license.txt
bitstream.checksum.fl_str_mv 4aa27a44bc94c9d06427df1b649e9b9e
bd3efa91386c1718a7f26a329fdcb468
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)
repository.mail.fl_str_mv bibliotecatede@uninove.br||bibliotecatede@uninove.br
_version_ 1811016869320065024