Efeito da vitamina 25(OH) D sobre a expressão de receptor toll-like e mediadores inflamatórios em monócitos frente ao ambiente urêmico
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/3024 |
Resumo: | The chronic kidney disease (CRD) is characterized by an inflammatory condition caused by the presence of uremic toxins in the patients plasma. This inflammatory state is associated with a high risk of cardiovascular diseases and infections. In CKD, there is a deficiency of vitamin 25 (OH) D, which has immunomodulatory properties, and the supplementation with it can decrease inflammation present in uremia. The aim of this study was to evaluate the effect of 25 (OH) D on inflammatory pathways such as toll like receptor 4 (TRL4), oxidative stress (ROS) and expression of vitamin D receptor (VDR), 1-α hydroxylase, 24 hydroxylase And inflammatory mediators in monocytes in the uremic environment. The human monocytes (U937 lineage) were treated with or without 25 (OH) D 50ng / ml for 24 hours, then they were incubated with 50% serum from healthy patients or uremic patient serum for 24 hours at 37 ° C and 5% CO2. Monocytes were characterized by the expression of CD14 +. TRL4, VDR, CYP24, Cyp27 and ROS were evaluated by flow cytometry. Cell culture supernatant ELISA was performed for IL-6, TNF-α, IL-10, catatelicidin, MCP-1 and NF-KB. We observed a high expression of TRL-4, IL-6, TNF-α, IL-10, cathelicidin and MCP-1 in monocytes incubated with uremic serum, compared when incubated with healthy serum. Treatment with 25 (OH) D was able to reduce the expression of TRL4, cathelicidin and MCP-1 against the uremic environment. There was no difference in the expression of VDR, CYP27 and CYP24 enzymes and NF-KB. We conclude that the uremic environment induces inflammation, increasing TRL4 and inflammatory mediators, treatment with vitamin D induces an improvement in the inflammatory parameters, resulting in less inflammation in the uremic environment. |
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Dalboni, Maria Aparecidahttp://lattes.cnpq.br/9818040147487320Dalboni, Maria Aparecidahttp://lattes.cnpq.br/9818040147487320Reis, Luciene Machado doshttp://lattes.cnpq.br/6070147721873955Dellê, Humbertohttp://lattes.cnpq.br/7435662740477057http://lattes.cnpq.br/6061325816552752Brito, Rodrigo Barbosa de Oliveira2022-08-01T14:28:30Z2017-02-10Brito, Rodrigo Barbosa de Oliveira. Efeito da vitamina 25(OH) D sobre a expressão de receptor toll-like e mediadores inflamatórios em monócitos frente ao ambiente urêmico. 2017. 57 f. Dissertação( Programa de Mestrado em Medicina) - Universidade Nove de Julho, São Paulo.http://bibliotecatede.uninove.br/handle/tede/3024The chronic kidney disease (CRD) is characterized by an inflammatory condition caused by the presence of uremic toxins in the patients plasma. This inflammatory state is associated with a high risk of cardiovascular diseases and infections. In CKD, there is a deficiency of vitamin 25 (OH) D, which has immunomodulatory properties, and the supplementation with it can decrease inflammation present in uremia. The aim of this study was to evaluate the effect of 25 (OH) D on inflammatory pathways such as toll like receptor 4 (TRL4), oxidative stress (ROS) and expression of vitamin D receptor (VDR), 1-α hydroxylase, 24 hydroxylase And inflammatory mediators in monocytes in the uremic environment. The human monocytes (U937 lineage) were treated with or without 25 (OH) D 50ng / ml for 24 hours, then they were incubated with 50% serum from healthy patients or uremic patient serum for 24 hours at 37 ° C and 5% CO2. Monocytes were characterized by the expression of CD14 +. TRL4, VDR, CYP24, Cyp27 and ROS were evaluated by flow cytometry. Cell culture supernatant ELISA was performed for IL-6, TNF-α, IL-10, catatelicidin, MCP-1 and NF-KB. We observed a high expression of TRL-4, IL-6, TNF-α, IL-10, cathelicidin and MCP-1 in monocytes incubated with uremic serum, compared when incubated with healthy serum. Treatment with 25 (OH) D was able to reduce the expression of TRL4, cathelicidin and MCP-1 against the uremic environment. There was no difference in the expression of VDR, CYP27 and CYP24 enzymes and NF-KB. We conclude that the uremic environment induces inflammation, increasing TRL4 and inflammatory mediators, treatment with vitamin D induces an improvement in the inflammatory parameters, resulting in less inflammation in the uremic environment.A doença renal crônica (DCR) é caracterizada por um estado inflamatório causado pela presença de toxinas urêmicas no plasma dos pacientes. Este estado inflamatório está associado com um elevado risco de doenças cardiovasculares e infecções. Na DCR, há uma deficiência de vitamina 25(OH)D, a qual possui propriedades imunomoduladoras, sendo que a suplementação com a mesma pode diminuir a inflamação presente na uremia. O objetivo deste estudo foi avaliar o efeito da 25(OH)D sobre vias inflamatórias como: receptor toll like 4 (TRL4), estresse oxidativo (ROS) e expressão do receptor de vitamina D (VDR), 1-α hidroxilase, 24 hidroxilase e mediadores inflamatórios em monócitos no ambiente urêmico. Os monócitos de linhagem U937 foram tratados com ou sem 25(OH)D 50ng/mL, durante 24 horas, depois estes foram incubados com 50% de soro de pacientes saudáveis ou soro de paciente urêmicos durante 24 horas a 37ºC e 5 de CO2. Os monócitos foram caracterizados pela expressão de CD14+. Os TRL4, VDR, CYP24, Cyp27 e o ROS foram avaliados por citometria de fluxo. Foi realizado ELISA do sobrenadante da cultura celular para IL-6, TNF-α, IL-10, catelicidina, MCP-1 e NFKB. Observamos uma alta expressão de TRL-4, IL-6, TNF-α, IL-10, catelicidina e MCP-1 em monócitos incubados com soro urêmico, comparados quando incubados com soro saudável. O tratamento com 25(OH)D foi capaz de reduzir a expressão de TRL4, catelicidina e MCP-1 frente ao ambiente urêmico. Não houve diferença na expressão do VDR, das enzimas CYP27 e CYP24 e NF-KB. Concluímos que o ambiente urêmico induz a inflamação, aumentando TRL4 e mediadores inflamatórios, o tratamento com vitamina D induz uma melhora nos parâmetros inflamatórios, resultando em menor inflamação no ambiente urêmico.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2022-08-01T14:28:30Z No. of bitstreams: 1 Rodrigo Barbosa de Oliveira Brito.pdf: 2018770 bytes, checksum: f685f896f207248db4f8b5354d35f7fd (MD5)Made available in DSpace on 2022-08-01T14:28:30Z (GMT). No. of bitstreams: 1 Rodrigo Barbosa de Oliveira Brito.pdf: 2018770 bytes, checksum: f685f896f207248db4f8b5354d35f7fd (MD5) Previous issue date: 2017-02-10application/pdfporUniversidade Nove de JulhoPrograma de Mestrado em MedicinaUNINOVEBrasilSaúdevitamina Dreceptor toll-likecitocinasuremiavitamintoll-like receptorcytokinesuremiaCIENCIAS DA SAUDEEfeito da vitamina 25(OH) D sobre a expressão de receptor toll-like e mediadores inflamatórios em monócitos frente ao ambiente urêmicoEffect of vitamin 25 (OH) D on toll-like receptor expression and inflammatory mediators in monocytes versus the uremic environmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALRodrigo Barbosa de Oliveira Brito.pdfRodrigo Barbosa de Oliveira Brito.pdfapplication/pdf2018770http://localhost:8080/tede/bitstream/tede/3024/2/Rodrigo+Barbosa+de+Oliveira+Brito.pdff685f896f207248db4f8b5354d35f7fdMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/3024/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/30242022-08-01 11:28:30.597oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2022-08-01T14:28:30Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito da vitamina 25(OH) D sobre a expressão de receptor toll-like e mediadores inflamatórios em monócitos frente ao ambiente urêmico |
dc.title.alternative.eng.fl_str_mv |
Effect of vitamin 25 (OH) D on toll-like receptor expression and inflammatory mediators in monocytes versus the uremic environment |
title |
Efeito da vitamina 25(OH) D sobre a expressão de receptor toll-like e mediadores inflamatórios em monócitos frente ao ambiente urêmico |
spellingShingle |
Efeito da vitamina 25(OH) D sobre a expressão de receptor toll-like e mediadores inflamatórios em monócitos frente ao ambiente urêmico Brito, Rodrigo Barbosa de Oliveira vitamina D receptor toll-like citocinas uremia vitamin toll-like receptor cytokines uremia CIENCIAS DA SAUDE |
title_short |
Efeito da vitamina 25(OH) D sobre a expressão de receptor toll-like e mediadores inflamatórios em monócitos frente ao ambiente urêmico |
title_full |
Efeito da vitamina 25(OH) D sobre a expressão de receptor toll-like e mediadores inflamatórios em monócitos frente ao ambiente urêmico |
title_fullStr |
Efeito da vitamina 25(OH) D sobre a expressão de receptor toll-like e mediadores inflamatórios em monócitos frente ao ambiente urêmico |
title_full_unstemmed |
Efeito da vitamina 25(OH) D sobre a expressão de receptor toll-like e mediadores inflamatórios em monócitos frente ao ambiente urêmico |
title_sort |
Efeito da vitamina 25(OH) D sobre a expressão de receptor toll-like e mediadores inflamatórios em monócitos frente ao ambiente urêmico |
author |
Brito, Rodrigo Barbosa de Oliveira |
author_facet |
Brito, Rodrigo Barbosa de Oliveira |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Dalboni, Maria Aparecida |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9818040147487320 |
dc.contributor.referee1.fl_str_mv |
Dalboni, Maria Aparecida |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/9818040147487320 |
dc.contributor.referee2.fl_str_mv |
Reis, Luciene Machado dos |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/6070147721873955 |
dc.contributor.referee3.fl_str_mv |
Dellê, Humberto |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/7435662740477057 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6061325816552752 |
dc.contributor.author.fl_str_mv |
Brito, Rodrigo Barbosa de Oliveira |
contributor_str_mv |
Dalboni, Maria Aparecida Dalboni, Maria Aparecida Reis, Luciene Machado dos Dellê, Humberto |
dc.subject.por.fl_str_mv |
vitamina D receptor toll-like citocinas uremia |
topic |
vitamina D receptor toll-like citocinas uremia vitamin toll-like receptor cytokines uremia CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
vitamin toll-like receptor cytokines uremia |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
The chronic kidney disease (CRD) is characterized by an inflammatory condition caused by the presence of uremic toxins in the patients plasma. This inflammatory state is associated with a high risk of cardiovascular diseases and infections. In CKD, there is a deficiency of vitamin 25 (OH) D, which has immunomodulatory properties, and the supplementation with it can decrease inflammation present in uremia. The aim of this study was to evaluate the effect of 25 (OH) D on inflammatory pathways such as toll like receptor 4 (TRL4), oxidative stress (ROS) and expression of vitamin D receptor (VDR), 1-α hydroxylase, 24 hydroxylase And inflammatory mediators in monocytes in the uremic environment. The human monocytes (U937 lineage) were treated with or without 25 (OH) D 50ng / ml for 24 hours, then they were incubated with 50% serum from healthy patients or uremic patient serum for 24 hours at 37 ° C and 5% CO2. Monocytes were characterized by the expression of CD14 +. TRL4, VDR, CYP24, Cyp27 and ROS were evaluated by flow cytometry. Cell culture supernatant ELISA was performed for IL-6, TNF-α, IL-10, catatelicidin, MCP-1 and NF-KB. We observed a high expression of TRL-4, IL-6, TNF-α, IL-10, cathelicidin and MCP-1 in monocytes incubated with uremic serum, compared when incubated with healthy serum. Treatment with 25 (OH) D was able to reduce the expression of TRL4, cathelicidin and MCP-1 against the uremic environment. There was no difference in the expression of VDR, CYP27 and CYP24 enzymes and NF-KB. We conclude that the uremic environment induces inflammation, increasing TRL4 and inflammatory mediators, treatment with vitamin D induces an improvement in the inflammatory parameters, resulting in less inflammation in the uremic environment. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-02-10 |
dc.date.accessioned.fl_str_mv |
2022-08-01T14:28:30Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Brito, Rodrigo Barbosa de Oliveira. Efeito da vitamina 25(OH) D sobre a expressão de receptor toll-like e mediadores inflamatórios em monócitos frente ao ambiente urêmico. 2017. 57 f. Dissertação( Programa de Mestrado em Medicina) - Universidade Nove de Julho, São Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/3024 |
identifier_str_mv |
Brito, Rodrigo Barbosa de Oliveira. Efeito da vitamina 25(OH) D sobre a expressão de receptor toll-like e mediadores inflamatórios em monócitos frente ao ambiente urêmico. 2017. 57 f. Dissertação( Programa de Mestrado em Medicina) - Universidade Nove de Julho, São Paulo. |
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http://bibliotecatede.uninove.br/handle/tede/3024 |
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