O ultrassom pulsado de baixa intensidade na regenera????o do m??sculo tibial anterior de rato: an??lise morfol??gica, organiza????o e deposi????o de col??geno e express??o de fatores regulat??rios miog??nicos

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Jacira Souza
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da Uninove
Texto Completo: http://bibliotecatede.uninove.br/handle/tede/1808
Resumo: The low-intensity pulsed ultrasound (LIPUS) has been used to promote muscle repair with better quality and in shorter time, however, there is no standardization for the parameters used in clinical practice. Thus, the aim of this study was to evaluate the effect of USPBI on the repair of skeletal muscle of rats after cryoinjury. Male Wistar rats (n=45) were divided into 3 groups: control; only injury; Injured and treated with LIPUS. The LIPUS application was performed daily, using the stationary mode, pulse 1: 4, 1 MHz frequency, intensity 0.4 W / cm2 for 3 minutes. The injured groups were euthanized at 1, 2, 3 and 7 days following injury induction. The tibialis anterior muscle (TA) was removed for morphological analysis and collagen remodeling, and the muscle sections stained with H&E and Picrosirus Red, respectively. Then, the slides were photographed and quantified using the program "Image J". The analysis of MyoD and myogenin gene expression was performed using real time PCR. The results showed that the USPBI promoted modulation of inflammatory responses with a decrease of inflammatory infiltrates after 1, 2, 3 and 7 days, and reduction of myonecrosis after 7 days, followed by an increase in the number of immature fibers after 3 and 7 days, and increase of blood vessels on days 2, 3 and 7 days. Regarding the deposition of collagen, the results showed better organization of the fibers in all experimental periods, and increased deposition of collagen fibers in the injured group and treated after 2 and 3 days. In addition, treatment with LIPUS promoted increased gene expression of MyoD reduction after 3 days and after 7 days. Regarding myogenin expression, the treated group showed increased expression after 7 days. In conclusion, the LIPUS induced positive effects on muscle repair process leading to reduced inflammation and myonecrosis, increased in the immature fibers and mature blood vessels, as well as modulation of Myod and miogenin in different periods.
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spelling Ferrari, Raquel Agnelli MesquitaFerrari, Raquel Agnelli MesquitaFran??a, Cristiane MirandaDurigan, Rita de C??ssia Marquetihttp://lattes.cnpq.br/0805770517626532Ribeiro, Jacira Souza2018-06-19T15:19:00Z2015-12-10Ribeiro, Jacira Souza. O ultrassom pulsado de baixa intensidade na regenera????o do m??sculo tibial anterior de rato: an??lise morfol??gica, organiza????o e deposi????o de col??geno e express??o de fatores regulat??rios miog??nicos. 2015. 53 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1808The low-intensity pulsed ultrasound (LIPUS) has been used to promote muscle repair with better quality and in shorter time, however, there is no standardization for the parameters used in clinical practice. Thus, the aim of this study was to evaluate the effect of USPBI on the repair of skeletal muscle of rats after cryoinjury. Male Wistar rats (n=45) were divided into 3 groups: control; only injury; Injured and treated with LIPUS. The LIPUS application was performed daily, using the stationary mode, pulse 1: 4, 1 MHz frequency, intensity 0.4 W / cm2 for 3 minutes. The injured groups were euthanized at 1, 2, 3 and 7 days following injury induction. The tibialis anterior muscle (TA) was removed for morphological analysis and collagen remodeling, and the muscle sections stained with H&E and Picrosirus Red, respectively. Then, the slides were photographed and quantified using the program "Image J". The analysis of MyoD and myogenin gene expression was performed using real time PCR. The results showed that the USPBI promoted modulation of inflammatory responses with a decrease of inflammatory infiltrates after 1, 2, 3 and 7 days, and reduction of myonecrosis after 7 days, followed by an increase in the number of immature fibers after 3 and 7 days, and increase of blood vessels on days 2, 3 and 7 days. Regarding the deposition of collagen, the results showed better organization of the fibers in all experimental periods, and increased deposition of collagen fibers in the injured group and treated after 2 and 3 days. In addition, treatment with LIPUS promoted increased gene expression of MyoD reduction after 3 days and after 7 days. Regarding myogenin expression, the treated group showed increased expression after 7 days. In conclusion, the LIPUS induced positive effects on muscle repair process leading to reduced inflammation and myonecrosis, increased in the immature fibers and mature blood vessels, as well as modulation of Myod and miogenin in different periods.O ultrassom pulsado de baixa intensidade (USPBI) tem sido utilizado por promover um reparo muscular de melhor qualidade e menor dura????o, por??m, n??o h?? padroniza????o quanto aos par??metros utilizados na pr??tica cl??nica. O objetivo deste estudo foi avaliar o efeito do USPBI sobre o reparo do m??sculo esquel??tico de rato ap??s crioles??o. Foram utilizados 45 ratos Wistar, machos, divididos em 3 grupos experimentais: controle; somente les??o; lesionado tratado com USPBI. A aplica????o de USPBI foi realizada diariamente ap??s indu????o da les??o, modo estacion??rio, pulsado 1:4, frequ??ncia 1 MHz, intensidade 0,4 W/cm2, durante 3 minutos. Os grupos lesionados foram eutanasiados ap??s 1, 2, 3 e 7 dias da indu????o da les??o. O m??sculo tibial anterior (TA) foi removido para an??lise morfol??gica e de remodelamento do col??geno, sendo os cortes corados com H&E e Picrosirius Red, respectivamente. As l??minas foram fotografadas e quantificadas com aux??lio do programa ???Image J???. A express??o g??nica de MyoD e miogenina foi obtida por PCR em tempo real. Os resultados evidenciaram que o USPBI promoveu modula????o da resposta inflamat??ria, havendo redu????o do infiltrado inflamat??rio ap??s 1, 2, 3 e 7 dias, e redu????o da mionecrose ap??s 7 dias, seguido pelo aumento no n??mero de fibras imaturas ap??s 3 e 7 dias, e aumento dos vasos sangu??neos nos dias 2, 3 e 7 dias. Em rela????o ?? deposi????o de col??geno, os resultados evidenciaram melhor organiza????o das fibras em todos os per??odos experimentais, al??m de aumento da deposi????o de fibras col??genas no grupo lesionado e tratado ap??s 2 e 3 dias. Al??m disso, o tratamento com USPBI promoveu aumento da express??o g??nica de MyoD ap??s 3 dias e redu????o ap??s 7 dias. Em rela????o a express??o de miogenina, o grupo tratado demonstrou aumento da express??o ap??s 7 dias. Em conclus??o, o USPBI nos par??metros utilizados induziu efeitos positivos ao processo de reparo muscular causando redu????o do processo inflamat??rio e mionecrose, aumento de fibras jovens vasos sangu??neos maduros, al??m de modula????o de MyoD e miogenina nos diferentes per??odos avaliados.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2018-06-19T15:19:00Z No. of bitstreams: 1 Jacira Souza Ribeiro.pdf: 1136563 bytes, checksum: 6f35b1abf4cd3ca62f8560ee50069169 (MD5)Made available in DSpace on 2018-06-19T15:19:00Z (GMT). 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dc.title.por.fl_str_mv O ultrassom pulsado de baixa intensidade na regenera????o do m??sculo tibial anterior de rato: an??lise morfol??gica, organiza????o e deposi????o de col??geno e express??o de fatores regulat??rios miog??nicos
dc.title.alternative.eng.fl_str_mv Ultrasound pulsed low intensity in the regeneration of the previous tibial muscle of mouse: morphological analysis, organization and deposition of collagen and expression of regulatory myogenic factors
title O ultrassom pulsado de baixa intensidade na regenera????o do m??sculo tibial anterior de rato: an??lise morfol??gica, organiza????o e deposi????o de col??geno e express??o de fatores regulat??rios miog??nicos
spellingShingle O ultrassom pulsado de baixa intensidade na regenera????o do m??sculo tibial anterior de rato: an??lise morfol??gica, organiza????o e deposi????o de col??geno e express??o de fatores regulat??rios miog??nicos
Ribeiro, Jacira Souza
reparo muscular
ultrassom pulsado de baixa intensidade
col??geno
c??lulas sat??lites
MyoD
miogenina
muscle repair
collagen
low-intensity pulsed ultrasound
satellite cells
MyoD
myogenin
CIENCIAS DA SAUDE
title_short O ultrassom pulsado de baixa intensidade na regenera????o do m??sculo tibial anterior de rato: an??lise morfol??gica, organiza????o e deposi????o de col??geno e express??o de fatores regulat??rios miog??nicos
title_full O ultrassom pulsado de baixa intensidade na regenera????o do m??sculo tibial anterior de rato: an??lise morfol??gica, organiza????o e deposi????o de col??geno e express??o de fatores regulat??rios miog??nicos
title_fullStr O ultrassom pulsado de baixa intensidade na regenera????o do m??sculo tibial anterior de rato: an??lise morfol??gica, organiza????o e deposi????o de col??geno e express??o de fatores regulat??rios miog??nicos
title_full_unstemmed O ultrassom pulsado de baixa intensidade na regenera????o do m??sculo tibial anterior de rato: an??lise morfol??gica, organiza????o e deposi????o de col??geno e express??o de fatores regulat??rios miog??nicos
title_sort O ultrassom pulsado de baixa intensidade na regenera????o do m??sculo tibial anterior de rato: an??lise morfol??gica, organiza????o e deposi????o de col??geno e express??o de fatores regulat??rios miog??nicos
author Ribeiro, Jacira Souza
author_facet Ribeiro, Jacira Souza
author_role author
dc.contributor.advisor1.fl_str_mv Ferrari, Raquel Agnelli Mesquita
dc.contributor.referee1.fl_str_mv Ferrari, Raquel Agnelli Mesquita
dc.contributor.referee2.fl_str_mv Fran??a, Cristiane Miranda
dc.contributor.referee3.fl_str_mv Durigan, Rita de C??ssia Marqueti
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/0805770517626532
dc.contributor.author.fl_str_mv Ribeiro, Jacira Souza
contributor_str_mv Ferrari, Raquel Agnelli Mesquita
Ferrari, Raquel Agnelli Mesquita
Fran??a, Cristiane Miranda
Durigan, Rita de C??ssia Marqueti
dc.subject.por.fl_str_mv reparo muscular
ultrassom pulsado de baixa intensidade
col??geno
c??lulas sat??lites
MyoD
miogenina
topic reparo muscular
ultrassom pulsado de baixa intensidade
col??geno
c??lulas sat??lites
MyoD
miogenina
muscle repair
collagen
low-intensity pulsed ultrasound
satellite cells
MyoD
myogenin
CIENCIAS DA SAUDE
dc.subject.eng.fl_str_mv muscle repair
collagen
low-intensity pulsed ultrasound
satellite cells
MyoD
myogenin
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE
description The low-intensity pulsed ultrasound (LIPUS) has been used to promote muscle repair with better quality and in shorter time, however, there is no standardization for the parameters used in clinical practice. Thus, the aim of this study was to evaluate the effect of USPBI on the repair of skeletal muscle of rats after cryoinjury. Male Wistar rats (n=45) were divided into 3 groups: control; only injury; Injured and treated with LIPUS. The LIPUS application was performed daily, using the stationary mode, pulse 1: 4, 1 MHz frequency, intensity 0.4 W / cm2 for 3 minutes. The injured groups were euthanized at 1, 2, 3 and 7 days following injury induction. The tibialis anterior muscle (TA) was removed for morphological analysis and collagen remodeling, and the muscle sections stained with H&E and Picrosirus Red, respectively. Then, the slides were photographed and quantified using the program "Image J". The analysis of MyoD and myogenin gene expression was performed using real time PCR. The results showed that the USPBI promoted modulation of inflammatory responses with a decrease of inflammatory infiltrates after 1, 2, 3 and 7 days, and reduction of myonecrosis after 7 days, followed by an increase in the number of immature fibers after 3 and 7 days, and increase of blood vessels on days 2, 3 and 7 days. Regarding the deposition of collagen, the results showed better organization of the fibers in all experimental periods, and increased deposition of collagen fibers in the injured group and treated after 2 and 3 days. In addition, treatment with LIPUS promoted increased gene expression of MyoD reduction after 3 days and after 7 days. Regarding myogenin expression, the treated group showed increased expression after 7 days. In conclusion, the LIPUS induced positive effects on muscle repair process leading to reduced inflammation and myonecrosis, increased in the immature fibers and mature blood vessels, as well as modulation of Myod and miogenin in different periods.
publishDate 2015
dc.date.issued.fl_str_mv 2015-12-10
dc.date.accessioned.fl_str_mv 2018-06-19T15:19:00Z
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dc.identifier.citation.fl_str_mv Ribeiro, Jacira Souza. O ultrassom pulsado de baixa intensidade na regenera????o do m??sculo tibial anterior de rato: an??lise morfol??gica, organiza????o e deposi????o de col??geno e express??o de fatores regulat??rios miog??nicos. 2015. 53 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.
dc.identifier.uri.fl_str_mv http://bibliotecatede.uninove.br/handle/tede/1808
identifier_str_mv Ribeiro, Jacira Souza. O ultrassom pulsado de baixa intensidade na regenera????o do m??sculo tibial anterior de rato: an??lise morfol??gica, organiza????o e deposi????o de col??geno e express??o de fatores regulat??rios miog??nicos. 2015. 53 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.
url http://bibliotecatede.uninove.br/handle/tede/1808
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language por
dc.relation.cnpq.fl_str_mv 8765449414823306929
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Universidade Nove de Julho
dc.publisher.program.fl_str_mv Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o
dc.publisher.initials.fl_str_mv UNINOVE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Sa??de
publisher.none.fl_str_mv Universidade Nove de Julho
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