Estudo do efeito do laser de baixa pot??ncia sobre a inflama????o pulmonar induzida por isquemia e reperfus??o intestinal

Detalhes bibliográficos
Autor(a) principal: Lima, Fl??via Mafra de
Data de Publicação: 2013
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da Uninove
Texto Completo: http://bibliotecatede.uninove.br/tede/handle/tede/910
Resumo: The systemic inflammation caused by ischemia and reperfusion (I/R-i) is characterized by generating , in exaggerated amounts of proinflammatory cytokines such as TNF- ?? , IL -1?? IL- 6 and NO. The injury that occurs in various organs after intestinal I/R-i is generally related to the occurrence of microvascular injury, increased plasma extravasation increased expression of adhesion molecules, recruitment and activation of inflammatory cells (mainly neutrophils). The anti-inflammatory effects of low-level laser (LLL) is known in many diseases, however, the mechanisms of its action are not yet well understood. The objective of this study is to describe the effect of low level laser on pulmonary inflammation in an experimental model of ischemia and reperfusion in order to investigate the possible mechanisms involved in these effects .Therefore, we divided the study into three stages: 1st) The goal was to evaluate the effect of laser in reactivity cholinergic dysfunction and release of inflammatory mediators in the lung after I/R-i 2nd) The objective was to assess whether the laser treatment could modulate the proinflammatory mediators and anti -inflammatory drugs in acute lung inflammation induced by I / Ri. 3rd) was conducting a study dose response model of lung inflammation induced by I / Ri in mice Methods: Wistar rats (150 - 200g) and C57/BL6 mice (20 - 25g) from the animal house of the Nove de Julho University. Each experimental group consisted of 5 animals each, as follows: naive group (baseline), sham (fake - operated), I/R-i, I/R + LBP -intestinal lymph in the region. The animals were anesthetized intraperitoneally with Ketamine / Xylazine (20 mg/kg-100 mg/kg) and after laparotomy and desviceriza????o intestinal ischemia was induced by clamping the superior mesenteric artery for 45 minutes. In the period after reperfusion (2 hours) the animals were anesthetized and sacrificed by exsanguination through the abdominal aorta. The animals were irradiated with a diode laser with a power of 30 mW and a wavelength of 660 nm irradiating an area of 0785 cm2 with a dose of 5.4 J in rats and (0.78 , 2.37 , 3.9, 5.8J ) in mice. Results Step 1: We note that, although laser treatment reduces edema and myeloperoxidase expression, it does not happen in all periods of reperfusion. This was also observed in the expression of ICAM- 1. But at the same time reducing TNF- ?? and iNOS laser increases the expression of IL -10 in the lungs of rats subjected to I / Ri Step 2: The results indicate that the laser reduces edema, MPO expression and TNF - ?? in the lungs when compared with control groups. laser increased the expression of IL - 10 in the lungs and 3rd stage: in mouse model, laser has also shown to be effective in reducing the concentration indirect neutrophils in the lung tissue at doses (2.37, 3.9, 5.8 J) and reduced mRNA expression and protein synthesis of (IL - 1??, TNF -??, IL - 6, IL - 10) Conclusion: we conclude that treatment with low level laser therapy is effective for lung inflammation induced by I / Ri at the doses studied. The dose that best submitted result was 3.9 J. Our results showed that the laser has reduced inflammatory cell migration and edema. Regarding the expression of iNOS laser has also shown to be effective in reducing its expression , and this indicates an effect of laser treatment on the reactivity of the trachea , since the I / Ri reactivity of the trachea is directly related to the concentrations of NO and IL-10. Regarding the expression of mRNA and protein synthesis of pro- inflammatory mediators (IL- 1, IL- 6 and TNF- ??) laser was shown to reduce these values , and enhance protein synthesis in IL-10
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spelling Carvalho, Regiane Albertini dehttp://lattes.cnpq.br/2515657728339113Cunha, Tatiana de SouzaCosta, Marcilia Silvahttp://lattes.cnpq.br/6704543089433679Vieira, Rodolfo de Paulahttp://lattes.cnpq.br/9213556008468472Carvalho, Paulo de Tarso Camillo dehttp://lattes.cnpq.br/2378784910179477CPF:31389770818http://lattes.cnpq.br/1514086166455057Lima, Fl??via Mafra de2015-04-22T17:20:47Z2014-12-032013-10-09LIMA, Fl??via Mafra de. Estudo do efeito do laser de baixa pot??ncia sobre a inflama????o pulmonar induzida por isquemia e reperfus??o intestinal. 2013. 53 f. Tese (Doutorado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2013.http://bibliotecatede.uninove.br/tede/handle/tede/910The systemic inflammation caused by ischemia and reperfusion (I/R-i) is characterized by generating , in exaggerated amounts of proinflammatory cytokines such as TNF- ?? , IL -1?? IL- 6 and NO. The injury that occurs in various organs after intestinal I/R-i is generally related to the occurrence of microvascular injury, increased plasma extravasation increased expression of adhesion molecules, recruitment and activation of inflammatory cells (mainly neutrophils). The anti-inflammatory effects of low-level laser (LLL) is known in many diseases, however, the mechanisms of its action are not yet well understood. The objective of this study is to describe the effect of low level laser on pulmonary inflammation in an experimental model of ischemia and reperfusion in order to investigate the possible mechanisms involved in these effects .Therefore, we divided the study into three stages: 1st) The goal was to evaluate the effect of laser in reactivity cholinergic dysfunction and release of inflammatory mediators in the lung after I/R-i 2nd) The objective was to assess whether the laser treatment could modulate the proinflammatory mediators and anti -inflammatory drugs in acute lung inflammation induced by I / Ri. 3rd) was conducting a study dose response model of lung inflammation induced by I / Ri in mice Methods: Wistar rats (150 - 200g) and C57/BL6 mice (20 - 25g) from the animal house of the Nove de Julho University. Each experimental group consisted of 5 animals each, as follows: naive group (baseline), sham (fake - operated), I/R-i, I/R + LBP -intestinal lymph in the region. The animals were anesthetized intraperitoneally with Ketamine / Xylazine (20 mg/kg-100 mg/kg) and after laparotomy and desviceriza????o intestinal ischemia was induced by clamping the superior mesenteric artery for 45 minutes. In the period after reperfusion (2 hours) the animals were anesthetized and sacrificed by exsanguination through the abdominal aorta. The animals were irradiated with a diode laser with a power of 30 mW and a wavelength of 660 nm irradiating an area of 0785 cm2 with a dose of 5.4 J in rats and (0.78 , 2.37 , 3.9, 5.8J ) in mice. Results Step 1: We note that, although laser treatment reduces edema and myeloperoxidase expression, it does not happen in all periods of reperfusion. This was also observed in the expression of ICAM- 1. But at the same time reducing TNF- ?? and iNOS laser increases the expression of IL -10 in the lungs of rats subjected to I / Ri Step 2: The results indicate that the laser reduces edema, MPO expression and TNF - ?? in the lungs when compared with control groups. laser increased the expression of IL - 10 in the lungs and 3rd stage: in mouse model, laser has also shown to be effective in reducing the concentration indirect neutrophils in the lung tissue at doses (2.37, 3.9, 5.8 J) and reduced mRNA expression and protein synthesis of (IL - 1??, TNF -??, IL - 6, IL - 10) Conclusion: we conclude that treatment with low level laser therapy is effective for lung inflammation induced by I / Ri at the doses studied. The dose that best submitted result was 3.9 J. Our results showed that the laser has reduced inflammatory cell migration and edema. Regarding the expression of iNOS laser has also shown to be effective in reducing its expression , and this indicates an effect of laser treatment on the reactivity of the trachea , since the I / Ri reactivity of the trachea is directly related to the concentrations of NO and IL-10. Regarding the expression of mRNA and protein synthesis of pro- inflammatory mediators (IL- 1, IL- 6 and TNF- ??) laser was shown to reduce these values , and enhance protein synthesis in IL-10A inflama????o sist??mica causada pela isquemia e reperfus??o intestinal (I/R-intestinal) caracteriza-se pela gera????o, em quantidades exageradas de citocinas pr??-inflamat??rias tais como, TNF-??, IL-1b, IL-6, e NO. A les??o que ocorre em diversos ??rg??os ap??s a I/R- intestinal est?? relacionada de forma geral ?? ocorr??ncia de les??o microvascular, aumento do extravasamento plasm??tico, aumento da express??o de mol??culas de ades??o, recrutamento e ativa????o de c??lulas inflamat??rias (notadamente neutr??filos).Os efeitos anti-inflamat??rios do laser de baixa pot??ncia (LBP) s??o conhecidos em diversas doen??as, entretanto, os mecanismos de sua a????o ainda n??o s??o bem compreendidos. Assim, o objetivo desse estudo ?? descrever o efeito do laser de baixa pot??ncia sobre a inflama????o pulmonar em modelo experimental de isquemia e reperfus??o intestinal, buscando investigar os poss??veis mecanismos envolvidos nesses efeitos. Para tanto, dividimos o estudo em 3 etapas: 1??) O objetivo foi avaliar o efeito do laser na disfun????o de reatividade colin??rgica e libera????o de mediadores inflamat??rios no pulm??o ap??s I/R-i. 2??) O objetivo do artigo foi avaliar se o tratamento com laser poderia modular os mediadores pr?? e anti-inflamat??rios na inflama????o pulmonar aguda induzida por I/R-i.. 3a) foi realizar um estudo dose resposta em modelo de inflama????o pulmonar induzido por I/R-i em camundongos M??todos: Foram utilizados ratos Wistar (150 -200g) e camundongos C57/BL6 (20-25g), provenientes do Biot??rio Central da Universidade Nove de Julho. Cada grupo experimental foi constitu??do de 5 animais, sendo os seguintes: grupo naive (basal); grupo sham (falso-operado); grupo I/R-intestinal; grupo I/R-intestinal + LBP na regi??o linf??tica. Os animais foram anestesiados por via intraperitoneal com Quetamina/Xilazina (20 mg/kg-100 mg/kg) e ap??s laparotomia mediana e desviceriza????o a isquemia intestinal foi induzida pelo pin??amento da art??ria mesent??rica superior por 45 minutos. No per??odo ap??s a reperfus??o (2 horas), os animais foram novamente anestesiados e sacrificados por dessangramento pela aorta abdominal. Os animais foram irradiados com um laser de diodo com pot??ncia de 30 mW e comprimento de onda de 660 nm irradiando uma ??rea de 0.785 cm2 com uma dose de 5.4 J em ratos e (0.78, 2.37, 3.9, 5.8J) em camundongos. Resultados da 1?? etapa: Observamos que, embora o tratamento com laser reduza o edema e a express??o de mieloperoxidase, isso n??o acontece em todos os per??odos de reperfus??o. Isso tamb??m foi observado na express??o de ICAM-1. Por??m ao mesmo tempo em que reduz TNF-?? e iNOS, o laser aumenta a express??o de IL-10 nos pulm??es de ratos submetidos ?? I/R-i, 2?? etapa: Os resultados indicam que o laser reduz o edema, express??o de MPO e TNF-?? nos pulm??es quando comparados com os grupos controle. o laser aumentou a express??o de IL-10 nos pulm??es e 3?? etapa: em modelo de camundongo, o laser tamb??m mostrou-se eficaz ao reduzir a concentra????o indireta de neutr??filos no tecido pulmonar nas doses de (2.37, 3.9, 5.8 J) e reduziu a express??o do RNAm e s??ntese proteica de (IL-1??, TNF-??, IL-6, IL-10) Conclus??o: Concluimos que o tratamento com laser de baixa pot??ncia ?? eficaz para inflama????o pulmonar induzida por I/R-i nas doses estudadas. A dose que melhor apresentou resultado foi de 3.9 J. Nossos resultados mostraram que o laser reduziu a migra????o de c??lulas inflamat??rias e o edema. Em rela????o ?? express??o de iNOS o laser tamb??m mostrou-se eficaz ao reduzir sua express??o, e isso indica um efeito do tratamento com laser na reatividade de traqu??ia, visto que na I/R-i a reatividade de traqu??ia est?? diretamente relacionada as concentra????es de NO e IL-10. Em rela????o ?? express??o do RNAm e s??ntese proteica de mediadores pr?? inflamat??rios (IL-1, IL-6 e TNF-??) o laser mostrou reduzir esses valores, e aumentar a s??ntese proteica de IL-10Made available in DSpace on 2015-04-22T17:20:47Z (GMT). No. of bitstreams: 1 Flavia Mafra de Lima.pdf: 664472 bytes, checksum: 6e68648e2dd514c8ba58efc3716e664b (MD5) Previous issue date: 2013-10-09application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBRSa??delaser de baixa pot??nciaisquemia e reperfus??o intestinalinflama????oARDSdose respostalow power laserischemia and reperfusioninflammationARDSdose responseCIENCIAS DA SAUDEEstudo do efeito do laser de baixa pot??ncia sobre a inflama????o pulmonar induzida por isquemia e reperfus??o intestinalEstudo do efeito do laser de baixa pot??ncia sobre a inflama????o pulmonar induzida por isquemia e reperfus??o intestinalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALFlavia Mafra de Lima.pdfapplication/pdf664472http://localhost:8080/tede/bitstream/tede/910/1/Flavia+Mafra+de+Lima.pdf6e68648e2dd514c8ba58efc3716e664bMD51tede/9102018-06-15 12:18:55.265oai:localhost:tede/910Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-15T15:18:55Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false
dc.title.por.fl_str_mv Estudo do efeito do laser de baixa pot??ncia sobre a inflama????o pulmonar induzida por isquemia e reperfus??o intestinal
dc.title.alternative.eng.fl_str_mv Estudo do efeito do laser de baixa pot??ncia sobre a inflama????o pulmonar induzida por isquemia e reperfus??o intestinal
title Estudo do efeito do laser de baixa pot??ncia sobre a inflama????o pulmonar induzida por isquemia e reperfus??o intestinal
spellingShingle Estudo do efeito do laser de baixa pot??ncia sobre a inflama????o pulmonar induzida por isquemia e reperfus??o intestinal
Lima, Fl??via Mafra de
laser de baixa pot??ncia
isquemia e reperfus??o intestinal
inflama????o
ARDS
dose resposta
low power laser
ischemia and reperfusion
inflammation
ARDS
dose response
CIENCIAS DA SAUDE
title_short Estudo do efeito do laser de baixa pot??ncia sobre a inflama????o pulmonar induzida por isquemia e reperfus??o intestinal
title_full Estudo do efeito do laser de baixa pot??ncia sobre a inflama????o pulmonar induzida por isquemia e reperfus??o intestinal
title_fullStr Estudo do efeito do laser de baixa pot??ncia sobre a inflama????o pulmonar induzida por isquemia e reperfus??o intestinal
title_full_unstemmed Estudo do efeito do laser de baixa pot??ncia sobre a inflama????o pulmonar induzida por isquemia e reperfus??o intestinal
title_sort Estudo do efeito do laser de baixa pot??ncia sobre a inflama????o pulmonar induzida por isquemia e reperfus??o intestinal
author Lima, Fl??via Mafra de
author_facet Lima, Fl??via Mafra de
author_role author
dc.contributor.advisor1.fl_str_mv Carvalho, Regiane Albertini de
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/2515657728339113
dc.contributor.referee1.fl_str_mv Cunha, Tatiana de Souza
dc.contributor.referee2.fl_str_mv Costa, Marcilia Silva
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/6704543089433679
dc.contributor.referee3.fl_str_mv Vieira, Rodolfo de Paula
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/9213556008468472
dc.contributor.referee4.fl_str_mv Carvalho, Paulo de Tarso Camillo de
dc.contributor.referee4Lattes.fl_str_mv http://lattes.cnpq.br/2378784910179477
dc.contributor.authorID.fl_str_mv CPF:31389770818
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/1514086166455057
dc.contributor.author.fl_str_mv Lima, Fl??via Mafra de
contributor_str_mv Carvalho, Regiane Albertini de
Cunha, Tatiana de Souza
Costa, Marcilia Silva
Vieira, Rodolfo de Paula
Carvalho, Paulo de Tarso Camillo de
dc.subject.por.fl_str_mv laser de baixa pot??ncia
isquemia e reperfus??o intestinal
inflama????o
ARDS
dose resposta
topic laser de baixa pot??ncia
isquemia e reperfus??o intestinal
inflama????o
ARDS
dose resposta
low power laser
ischemia and reperfusion
inflammation
ARDS
dose response
CIENCIAS DA SAUDE
dc.subject.eng.fl_str_mv low power laser
ischemia and reperfusion
inflammation
ARDS
dose response
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE
description The systemic inflammation caused by ischemia and reperfusion (I/R-i) is characterized by generating , in exaggerated amounts of proinflammatory cytokines such as TNF- ?? , IL -1?? IL- 6 and NO. The injury that occurs in various organs after intestinal I/R-i is generally related to the occurrence of microvascular injury, increased plasma extravasation increased expression of adhesion molecules, recruitment and activation of inflammatory cells (mainly neutrophils). The anti-inflammatory effects of low-level laser (LLL) is known in many diseases, however, the mechanisms of its action are not yet well understood. The objective of this study is to describe the effect of low level laser on pulmonary inflammation in an experimental model of ischemia and reperfusion in order to investigate the possible mechanisms involved in these effects .Therefore, we divided the study into three stages: 1st) The goal was to evaluate the effect of laser in reactivity cholinergic dysfunction and release of inflammatory mediators in the lung after I/R-i 2nd) The objective was to assess whether the laser treatment could modulate the proinflammatory mediators and anti -inflammatory drugs in acute lung inflammation induced by I / Ri. 3rd) was conducting a study dose response model of lung inflammation induced by I / Ri in mice Methods: Wistar rats (150 - 200g) and C57/BL6 mice (20 - 25g) from the animal house of the Nove de Julho University. Each experimental group consisted of 5 animals each, as follows: naive group (baseline), sham (fake - operated), I/R-i, I/R + LBP -intestinal lymph in the region. The animals were anesthetized intraperitoneally with Ketamine / Xylazine (20 mg/kg-100 mg/kg) and after laparotomy and desviceriza????o intestinal ischemia was induced by clamping the superior mesenteric artery for 45 minutes. In the period after reperfusion (2 hours) the animals were anesthetized and sacrificed by exsanguination through the abdominal aorta. The animals were irradiated with a diode laser with a power of 30 mW and a wavelength of 660 nm irradiating an area of 0785 cm2 with a dose of 5.4 J in rats and (0.78 , 2.37 , 3.9, 5.8J ) in mice. Results Step 1: We note that, although laser treatment reduces edema and myeloperoxidase expression, it does not happen in all periods of reperfusion. This was also observed in the expression of ICAM- 1. But at the same time reducing TNF- ?? and iNOS laser increases the expression of IL -10 in the lungs of rats subjected to I / Ri Step 2: The results indicate that the laser reduces edema, MPO expression and TNF - ?? in the lungs when compared with control groups. laser increased the expression of IL - 10 in the lungs and 3rd stage: in mouse model, laser has also shown to be effective in reducing the concentration indirect neutrophils in the lung tissue at doses (2.37, 3.9, 5.8 J) and reduced mRNA expression and protein synthesis of (IL - 1??, TNF -??, IL - 6, IL - 10) Conclusion: we conclude that treatment with low level laser therapy is effective for lung inflammation induced by I / Ri at the doses studied. The dose that best submitted result was 3.9 J. Our results showed that the laser has reduced inflammatory cell migration and edema. Regarding the expression of iNOS laser has also shown to be effective in reducing its expression , and this indicates an effect of laser treatment on the reactivity of the trachea , since the I / Ri reactivity of the trachea is directly related to the concentrations of NO and IL-10. Regarding the expression of mRNA and protein synthesis of pro- inflammatory mediators (IL- 1, IL- 6 and TNF- ??) laser was shown to reduce these values , and enhance protein synthesis in IL-10
publishDate 2013
dc.date.issued.fl_str_mv 2013-10-09
dc.date.available.fl_str_mv 2014-12-03
dc.date.accessioned.fl_str_mv 2015-04-22T17:20:47Z
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dc.identifier.citation.fl_str_mv LIMA, Fl??via Mafra de. Estudo do efeito do laser de baixa pot??ncia sobre a inflama????o pulmonar induzida por isquemia e reperfus??o intestinal. 2013. 53 f. Tese (Doutorado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2013.
dc.identifier.uri.fl_str_mv http://bibliotecatede.uninove.br/tede/handle/tede/910
identifier_str_mv LIMA, Fl??via Mafra de. Estudo do efeito do laser de baixa pot??ncia sobre a inflama????o pulmonar induzida por isquemia e reperfus??o intestinal. 2013. 53 f. Tese (Doutorado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2013.
url http://bibliotecatede.uninove.br/tede/handle/tede/910
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