Estudo comparativo do efeito da fotobiomodulação vascular com a local em modelo experimental de rinite alérgica induzida por ovoalbumina

Detalhes bibliográficos
Autor(a) principal: Schapochnik, Adriana
Data de Publicação: 2021
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da Uninove
Texto Completo: http://bibliotecatede.uninove.br/handle/tede/2877
Resumo: Allergic Rhinitis (AR) is defined as inflammation and/or dysfunction of the nasal mucosa and is a global health problem that has a significant impact on quality of life and the burden of medical care patient education based on better communication can facilitate the control and prevention of allergic rhinitis. The symptoms of AR negatively affect the patient and decrease their productivity. The goal of AR treatment is symptom relief and includes nasal saline irrigation, oral and intranasal decongestants, oral antihistamines, intranasal corticosteroids, leukotriene receptor antagonists, and immunotherapy. However, some of these drugs have considerable side effects, and in more severe cases they are not effective. Thus, Photobiomodulation (PBM) emerges as an adjuvant therapy, without adverse effects and presents good results for several diseases with an inflammatory character, including those of the respiratory tract. Considering that AR is not just a disorder located in the nose and nasal cavities and has a systemic component, this project aims to compare the effects of Vascular PBM (VPBM) in the caudal and Local artery (LPBM) under the nostril with a device. LED (Light Emitting Diode) on the development of AR. For this purpose, adult male Wistar rats were submitted or not to RA by intradermal injections of Ovalbumin (OVA) plus aluminum hydroxide as an adjuvant dissolved in saline solution (from day 1 to 13). After immunization, nasal challenge was performed from day 14 to 21 through daily intranasal instillation of OVA. Rats treated with VPBM and LPBM were irradiated with LED apparatus in the tail artery and nostril for 3 consecutive days immediately after OVA challenge. Our results showed that treatment with VPBM, reduced the level and gene expression of IL-4, increased IL-10 without altering IL-5 and IFN-gamma, and reduced Th2, a cytokine which contribute to improving the symptoms of AR. In this context, the proposed study provided subsidies to propose an effective therapeutic alternative for the treatment of AR with LPMB and not VPMB which does not show any additive effect that justifies its use.
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spelling Franco, Adriana Lino dos SantosFranco, Adriana Lino dos SantosMarcos, Rodrigo LabatFerreira, Caroline MarcantonioRodrigues, Maria Fernanda Setúbal DestroZamuner, Stella Reginahttp://lattes.cnpq.br/1819834885358331Schapochnik, Adriana2022-04-12T18:25:08Z2021-12-08Schapochnik, Adriana. Estudo comparativo do efeito da fotobiomodulação vascular com a local em modelo experimental de rinite alérgica induzida por ovoalbumina. 2021. 68 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo.http://bibliotecatede.uninove.br/handle/tede/2877Allergic Rhinitis (AR) is defined as inflammation and/or dysfunction of the nasal mucosa and is a global health problem that has a significant impact on quality of life and the burden of medical care patient education based on better communication can facilitate the control and prevention of allergic rhinitis. The symptoms of AR negatively affect the patient and decrease their productivity. The goal of AR treatment is symptom relief and includes nasal saline irrigation, oral and intranasal decongestants, oral antihistamines, intranasal corticosteroids, leukotriene receptor antagonists, and immunotherapy. However, some of these drugs have considerable side effects, and in more severe cases they are not effective. Thus, Photobiomodulation (PBM) emerges as an adjuvant therapy, without adverse effects and presents good results for several diseases with an inflammatory character, including those of the respiratory tract. Considering that AR is not just a disorder located in the nose and nasal cavities and has a systemic component, this project aims to compare the effects of Vascular PBM (VPBM) in the caudal and Local artery (LPBM) under the nostril with a device. LED (Light Emitting Diode) on the development of AR. For this purpose, adult male Wistar rats were submitted or not to RA by intradermal injections of Ovalbumin (OVA) plus aluminum hydroxide as an adjuvant dissolved in saline solution (from day 1 to 13). After immunization, nasal challenge was performed from day 14 to 21 through daily intranasal instillation of OVA. Rats treated with VPBM and LPBM were irradiated with LED apparatus in the tail artery and nostril for 3 consecutive days immediately after OVA challenge. Our results showed that treatment with VPBM, reduced the level and gene expression of IL-4, increased IL-10 without altering IL-5 and IFN-gamma, and reduced Th2, a cytokine which contribute to improving the symptoms of AR. In this context, the proposed study provided subsidies to propose an effective therapeutic alternative for the treatment of AR with LPMB and not VPMB which does not show any additive effect that justifies its use.A Rinite Alérgica (RA) é definida como inflamação e/ou disfunção da mucosa nasal e é um problema de saúde mundial que gera um impacto significativo na qualidade de vida e ônus da assistência médica e a educação ao paciente, baseada em informativos, pode facilitar o controle a prevenção da rinite alérgica. Os sintomas de RA afeta negativamente o paciente e diminui sua produtividade. O objetivo do tratamento da RA é o alívio dos sintomas e incluem irrigação de salina nasal, descongestionantes orais e intranasais, anti-histamínicos orais, corticosteroides intranasais, antagonistas dos receptores de leucotrienos e imunoterapia. No entanto, alguns destes fármacos apresentam efeitos colaterais consideráveis, e em casos mais graves não são eficazes. Assim, a Fotobiomodulação (FBM) emerge como uma terapia coadjuvante, sem efeitos adversos e apresenta bons resultados para diversas doenças com caráter inflamatório, incluindo as do trato respiratório. Tendo em vista que a RA não é apenas um distúrbio localizado no nariz e nas fossas nasais e apresenta componente sistêmico, o presente projeto teve o objetivo de comparar os efeitos da FBM Vascular (FBMV) na Artéria Caudal e Local (FBML) sob a narina com aparelho de LED (Light Emitting Diode) sobre o desenvolvimento da RA. Para tanto, ratos Wistar machos adultos foram submetidos ou não a RA por meio de injeções intradérmicas de Ovoalbumina (OVA) acrescida de hidróxido de alumínio como adjuvante dissolvidos em solução salina (do dia 1 ao 13). Após a imunização, o desafio nasal foi realizado do dia 14 ao 21 através da instilação intranasal diária de OVA. Os ratos tratados com FBMV e FBML foram irradiados com aparelho de LED na artéria caudal e narina durante 3 dias consecutivos imediatamente após a provocação com OVA. Nossos resultados mostraram que o tratamento com FBML, e não a FBMV, reduziu o nível e a expressão gênica de IL-4, aumentou a IL-10 sem alterar IL-5 e IFN-gama e reduziu as citocinas Th2, a qual contribui para melhorar os sintomas de RA. Nesse contexto, o estudo proposto forneceu subsídios para propor alternativa terapêutica eficaz para o tratamento da RA com FBML e não FBMV a qual não evidencia nenhum efeito aditivo que justifique seu uso.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2022-04-12T18:25:08Z No. of bitstreams: 1 Adriana Schapochnik.pdf: 2483128 bytes, checksum: bb2d3412bd81dd02118dc8656627526f (MD5)Made available in DSpace on 2022-04-12T18:25:08Z (GMT). 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dc.title.por.fl_str_mv Estudo comparativo do efeito da fotobiomodulação vascular com a local em modelo experimental de rinite alérgica induzida por ovoalbumina
dc.title.alternative.eng.fl_str_mv Comparative study of the effect of vascular and local photobiomodulation in an experimental model of allergic rhinitis induced by Ovoalbumin
title Estudo comparativo do efeito da fotobiomodulação vascular com a local em modelo experimental de rinite alérgica induzida por ovoalbumina
spellingShingle Estudo comparativo do efeito da fotobiomodulação vascular com a local em modelo experimental de rinite alérgica induzida por ovoalbumina
Schapochnik, Adriana
inflamação
doenças alérgicas
citocinas
LLLT
laser de baixa intensidade
LED
fototerapia
ILIB
allergic rhinitis
inflammation
cytokines
photobiomodulation
laser
LED
phototherapy
ILIB
CIENCIAS DA SAUDE
title_short Estudo comparativo do efeito da fotobiomodulação vascular com a local em modelo experimental de rinite alérgica induzida por ovoalbumina
title_full Estudo comparativo do efeito da fotobiomodulação vascular com a local em modelo experimental de rinite alérgica induzida por ovoalbumina
title_fullStr Estudo comparativo do efeito da fotobiomodulação vascular com a local em modelo experimental de rinite alérgica induzida por ovoalbumina
title_full_unstemmed Estudo comparativo do efeito da fotobiomodulação vascular com a local em modelo experimental de rinite alérgica induzida por ovoalbumina
title_sort Estudo comparativo do efeito da fotobiomodulação vascular com a local em modelo experimental de rinite alérgica induzida por ovoalbumina
author Schapochnik, Adriana
author_facet Schapochnik, Adriana
author_role author
dc.contributor.advisor1.fl_str_mv Franco, Adriana Lino dos Santos
dc.contributor.referee1.fl_str_mv Franco, Adriana Lino dos Santos
dc.contributor.referee2.fl_str_mv Marcos, Rodrigo Labat
dc.contributor.referee3.fl_str_mv Ferreira, Caroline Marcantonio
dc.contributor.referee4.fl_str_mv Rodrigues, Maria Fernanda Setúbal Destro
dc.contributor.referee5.fl_str_mv Zamuner, Stella Regina
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/1819834885358331
dc.contributor.author.fl_str_mv Schapochnik, Adriana
contributor_str_mv Franco, Adriana Lino dos Santos
Franco, Adriana Lino dos Santos
Marcos, Rodrigo Labat
Ferreira, Caroline Marcantonio
Rodrigues, Maria Fernanda Setúbal Destro
Zamuner, Stella Regina
dc.subject.por.fl_str_mv inflamação
doenças alérgicas
citocinas
LLLT
laser de baixa intensidade
LED
fototerapia
ILIB
topic inflamação
doenças alérgicas
citocinas
LLLT
laser de baixa intensidade
LED
fototerapia
ILIB
allergic rhinitis
inflammation
cytokines
photobiomodulation
laser
LED
phototherapy
ILIB
CIENCIAS DA SAUDE
dc.subject.eng.fl_str_mv allergic rhinitis
inflammation
cytokines
photobiomodulation
laser
LED
phototherapy
ILIB
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE
description Allergic Rhinitis (AR) is defined as inflammation and/or dysfunction of the nasal mucosa and is a global health problem that has a significant impact on quality of life and the burden of medical care patient education based on better communication can facilitate the control and prevention of allergic rhinitis. The symptoms of AR negatively affect the patient and decrease their productivity. The goal of AR treatment is symptom relief and includes nasal saline irrigation, oral and intranasal decongestants, oral antihistamines, intranasal corticosteroids, leukotriene receptor antagonists, and immunotherapy. However, some of these drugs have considerable side effects, and in more severe cases they are not effective. Thus, Photobiomodulation (PBM) emerges as an adjuvant therapy, without adverse effects and presents good results for several diseases with an inflammatory character, including those of the respiratory tract. Considering that AR is not just a disorder located in the nose and nasal cavities and has a systemic component, this project aims to compare the effects of Vascular PBM (VPBM) in the caudal and Local artery (LPBM) under the nostril with a device. LED (Light Emitting Diode) on the development of AR. For this purpose, adult male Wistar rats were submitted or not to RA by intradermal injections of Ovalbumin (OVA) plus aluminum hydroxide as an adjuvant dissolved in saline solution (from day 1 to 13). After immunization, nasal challenge was performed from day 14 to 21 through daily intranasal instillation of OVA. Rats treated with VPBM and LPBM were irradiated with LED apparatus in the tail artery and nostril for 3 consecutive days immediately after OVA challenge. Our results showed that treatment with VPBM, reduced the level and gene expression of IL-4, increased IL-10 without altering IL-5 and IFN-gamma, and reduced Th2, a cytokine which contribute to improving the symptoms of AR. In this context, the proposed study provided subsidies to propose an effective therapeutic alternative for the treatment of AR with LPMB and not VPMB which does not show any additive effect that justifies its use.
publishDate 2021
dc.date.issued.fl_str_mv 2021-12-08
dc.date.accessioned.fl_str_mv 2022-04-12T18:25:08Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
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dc.identifier.citation.fl_str_mv Schapochnik, Adriana. Estudo comparativo do efeito da fotobiomodulação vascular com a local em modelo experimental de rinite alérgica induzida por ovoalbumina. 2021. 68 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo.
dc.identifier.uri.fl_str_mv http://bibliotecatede.uninove.br/handle/tede/2877
identifier_str_mv Schapochnik, Adriana. Estudo comparativo do efeito da fotobiomodulação vascular com a local em modelo experimental de rinite alérgica induzida por ovoalbumina. 2021. 68 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo.
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