Estudo do comportamento da mec??nica vascular no processo de descelulariza????o pulmonar
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/1830 |
Resumo: | Organ biofabrication is a potential future alternative for obtaining viable organs for transplantation. Achieving intact scaffolds to be recellularized is a key step in lung bioengineering. The decellularizing agent perfusion technique via the pulmonary artery (PA) has been shown very effective in the process however; vascular perfusion pressure and flow vary along the pulmonary decellularization process. These factors are not fully understood it being very important in the optimization process, ensuring the integrity of the scaffold. The objectives were to characterize the pressure / pulmonary vascular flow associated with variation in vascular resistance (VR), according to the control of the infusion (pressure or flow) at the time of the infusion of different decellularizing agents in PA and determine the VR's behavior in relation to different pressures of lung inflation (tracheal pressure) and perfusion (pulmonary artery). For the first study, were used 43 lungs of the healthy mice (C57/BL6) with 7???8 weeks old and in the second study, lungs of the 5 healthy rat (Sprague- Dawley) with 7-8 weeks old. In the first study, after excision and tracheal cannulation, lungs were inflated at 10 cmH2O airway pressure and subjected to conventional decellularization process being perfused through PA. For the second study, the decellularized lungs were subjected to variations in tracheal pressure (0 to 15 cmH2O) and vascular pressure (5 to 30 cmH2O). Pressure (PPA) and flow (V???PA) at the pulmonary artery were continuously measured. The VR (VR=PPA/V???PA) considerably varied throughout lung decellularization, particularly for pressure controlled perfusion, as compared with flow-controlled perfusion. This study shows that monitoring perfusion mechanics throughout decellularization provides information relevant for optimizing the process time while ensuring that vascular pressure is kept within a safety range to preserve the organ scaffold integrity. Moreover, arterial lung pressure has more influence on behavior of vascular resistance in decellularized lungs than positive airway pressure, providing information that could be relevant for future cell repopulation by using the vascular resistance as a facilitator cell distribution throughout pulmonary circuit. |
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Oliveira, Luis Vicente Franco deOliveira, Luis Vicente Franco deCarvalho, Flavio Aimbire Soares deSaraiva, Beatriz MangueiraSampaio, Luciana Maria Malos??Vieira, Rodolfo de Paulahttp://lattes.cnpq.br/2990647294511164Palma, Renata Kelly da2018-06-21T19:41:07Z2015-10-07Palma, Renata Kelly da. Estudo do comportamento da mec??nica vascular no processo de descelulariza????o pulmonar. 2015. 125 f. Tese( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1830Organ biofabrication is a potential future alternative for obtaining viable organs for transplantation. Achieving intact scaffolds to be recellularized is a key step in lung bioengineering. The decellularizing agent perfusion technique via the pulmonary artery (PA) has been shown very effective in the process however; vascular perfusion pressure and flow vary along the pulmonary decellularization process. These factors are not fully understood it being very important in the optimization process, ensuring the integrity of the scaffold. The objectives were to characterize the pressure / pulmonary vascular flow associated with variation in vascular resistance (VR), according to the control of the infusion (pressure or flow) at the time of the infusion of different decellularizing agents in PA and determine the VR's behavior in relation to different pressures of lung inflation (tracheal pressure) and perfusion (pulmonary artery). For the first study, were used 43 lungs of the healthy mice (C57/BL6) with 7???8 weeks old and in the second study, lungs of the 5 healthy rat (Sprague- Dawley) with 7-8 weeks old. In the first study, after excision and tracheal cannulation, lungs were inflated at 10 cmH2O airway pressure and subjected to conventional decellularization process being perfused through PA. For the second study, the decellularized lungs were subjected to variations in tracheal pressure (0 to 15 cmH2O) and vascular pressure (5 to 30 cmH2O). Pressure (PPA) and flow (V???PA) at the pulmonary artery were continuously measured. The VR (VR=PPA/V???PA) considerably varied throughout lung decellularization, particularly for pressure controlled perfusion, as compared with flow-controlled perfusion. This study shows that monitoring perfusion mechanics throughout decellularization provides information relevant for optimizing the process time while ensuring that vascular pressure is kept within a safety range to preserve the organ scaffold integrity. Moreover, arterial lung pressure has more influence on behavior of vascular resistance in decellularized lungs than positive airway pressure, providing information that could be relevant for future cell repopulation by using the vascular resistance as a facilitator cell distribution throughout pulmonary circuit.A gera????o artificial de ??rg??os ?? uma alternativa potencial na obten????o de ??rg??os vi??veis para o transplante humano. Obter scaffolds perfeitos para serem recelularizados ?? um grande desafio e um passo fundamental na bioengenharia de pulm??es. A t??cnica de perfus??o de agentes descelularizantes atrav??s da art??ria pulmonar (AP) tem se apresentado muito eficaz no processo entretanto, a press??o e o fluxo de perfus??o vascular variam ao longo do processo de descelulariza????o pulmonar. Estes fatores ainda n??o se encontram totalmente compreendidos, sendo muito importantes na otimiza????o do processo, assegurando a integridade dos scaffolds. Os objetivos foram caracterizar a rela????o press??o/fluxo vascular pulmonar associado a varia????o de resist??ncia vascular (RV), de acordo com o controle da perfus??o (press??o ou fluxo) no momento da infus??o de diferentes agentes descelularizantes na AP e determinar o comportamento da RV em rela????o a diferentes press??es de insufla????o pulmonar (press??o traqueal) e de perfus??o (art??ria pulmonar). Para o primeiro estudo, foram utilizados 43 pulm??es de camundongos machos saud??veis (C57/BL6) com idade de 7-8 semanas e no segundo estudo, pulm??es de 5 ratos machos saudav??is (Sprague- Dawley) com idade de 7-8 semanas. No primeiro estudo, ap??s excis??o e canula????o da traqueia e da art??ria pulmonar, os pulm??es foram insuflados a uma press??o de 10 cmH2O na via a??rea e submetidos ao processo convencional de descelulariza????o sendo perfundidos atrav??s da AP. Para o segundo estudo, os pulm??es descelularizados foram submetidos a varia????es de press??o traqueal (0 ?? 15 cmH2O) e press??o vascular (5 ?? 30 cmH2O). A press??o na art??ria pulmonar (PPA) e o fluxo da art??ria pulmonar (V???PA) foram continuamente mensurados. A RV (Rv=PPA/V`PA.Rv) variou consideravelmente ao longo do processo de descelulariza????o pulmonar, particularmente na perfus??o por press??o controlada, quando comparado a perfus??o controlada por fluxo. Concluimos que o monitoramento da mec??nica de perfus??o ao longo do processo de descelulariza????o fornece informa????es relevantes na otimiza????o do processo, assegurando um limite para press??o vascular, preservando a integridade do scaffold. Somado a estes achados um resultado relevante demonstrou que a press??o arterial pulmonar tem mais influ??ncia no comportamento da RV nos pulm??es descelularizados do que press??o positiva nas vias a??reas, fornecendo informa????es importantes para futuro manejo no processo de recelulariza????o utilizando a RV como um facilitador na distribui????o celular atrav??s do arcabou??o celular pulmonar.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2018-06-21T19:41:07Z No. of bitstreams: 1 Renata Kelly da Palma.pdf: 5650038 bytes, checksum: 27a64e62c897fb5ab5e43cf7a98e3250 (MD5)Made available in DSpace on 2018-06-21T19:41:07Z (GMT). No. of bitstreams: 1 Renata Kelly da Palma.pdf: 5650038 bytes, checksum: 27a64e62c897fb5ab5e43cf7a98e3250 (MD5) Previous issue date: 2015-10-07application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBrasilSa??demec??nica vascularpulm??esbioengenharia de ??rg??osdescelulariza????operfus??o pulmonarvascular mechanicslungsbioengineeringdecellularizationlung perfusionCIENCIAS DA SAUDEEstudo do comportamento da mec??nica vascular no processo de descelulariza????o pulmonarStudy of behavior of vascular mecanic in decellularization lunginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALRenata Kelly da Palma.pdfRenata Kelly da Palma.pdfapplication/pdf5650038http://localhost:8080/tede/bitstream/tede/1830/2/Renata+Kelly+da+Palma.pdf27a64e62c897fb5ab5e43cf7a98e3250MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/1830/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/18302018-06-21 16:41:07.501oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-21T19:41:07Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Estudo do comportamento da mec??nica vascular no processo de descelulariza????o pulmonar |
dc.title.alternative.eng.fl_str_mv |
Study of behavior of vascular mecanic in decellularization lung |
title |
Estudo do comportamento da mec??nica vascular no processo de descelulariza????o pulmonar |
spellingShingle |
Estudo do comportamento da mec??nica vascular no processo de descelulariza????o pulmonar Palma, Renata Kelly da mec??nica vascular pulm??es bioengenharia de ??rg??os descelulariza????o perfus??o pulmonar vascular mechanics lungs bioengineering decellularization lung perfusion CIENCIAS DA SAUDE |
title_short |
Estudo do comportamento da mec??nica vascular no processo de descelulariza????o pulmonar |
title_full |
Estudo do comportamento da mec??nica vascular no processo de descelulariza????o pulmonar |
title_fullStr |
Estudo do comportamento da mec??nica vascular no processo de descelulariza????o pulmonar |
title_full_unstemmed |
Estudo do comportamento da mec??nica vascular no processo de descelulariza????o pulmonar |
title_sort |
Estudo do comportamento da mec??nica vascular no processo de descelulariza????o pulmonar |
author |
Palma, Renata Kelly da |
author_facet |
Palma, Renata Kelly da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Oliveira, Luis Vicente Franco de |
dc.contributor.referee1.fl_str_mv |
Oliveira, Luis Vicente Franco de |
dc.contributor.referee2.fl_str_mv |
Carvalho, Flavio Aimbire Soares de |
dc.contributor.referee3.fl_str_mv |
Saraiva, Beatriz Mangueira |
dc.contributor.referee4.fl_str_mv |
Sampaio, Luciana Maria Malos?? |
dc.contributor.referee5.fl_str_mv |
Vieira, Rodolfo de Paula |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2990647294511164 |
dc.contributor.author.fl_str_mv |
Palma, Renata Kelly da |
contributor_str_mv |
Oliveira, Luis Vicente Franco de Oliveira, Luis Vicente Franco de Carvalho, Flavio Aimbire Soares de Saraiva, Beatriz Mangueira Sampaio, Luciana Maria Malos?? Vieira, Rodolfo de Paula |
dc.subject.por.fl_str_mv |
mec??nica vascular pulm??es bioengenharia de ??rg??os descelulariza????o perfus??o pulmonar |
topic |
mec??nica vascular pulm??es bioengenharia de ??rg??os descelulariza????o perfus??o pulmonar vascular mechanics lungs bioengineering decellularization lung perfusion CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
vascular mechanics lungs bioengineering decellularization lung perfusion |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Organ biofabrication is a potential future alternative for obtaining viable organs for transplantation. Achieving intact scaffolds to be recellularized is a key step in lung bioengineering. The decellularizing agent perfusion technique via the pulmonary artery (PA) has been shown very effective in the process however; vascular perfusion pressure and flow vary along the pulmonary decellularization process. These factors are not fully understood it being very important in the optimization process, ensuring the integrity of the scaffold. The objectives were to characterize the pressure / pulmonary vascular flow associated with variation in vascular resistance (VR), according to the control of the infusion (pressure or flow) at the time of the infusion of different decellularizing agents in PA and determine the VR's behavior in relation to different pressures of lung inflation (tracheal pressure) and perfusion (pulmonary artery). For the first study, were used 43 lungs of the healthy mice (C57/BL6) with 7???8 weeks old and in the second study, lungs of the 5 healthy rat (Sprague- Dawley) with 7-8 weeks old. In the first study, after excision and tracheal cannulation, lungs were inflated at 10 cmH2O airway pressure and subjected to conventional decellularization process being perfused through PA. For the second study, the decellularized lungs were subjected to variations in tracheal pressure (0 to 15 cmH2O) and vascular pressure (5 to 30 cmH2O). Pressure (PPA) and flow (V???PA) at the pulmonary artery were continuously measured. The VR (VR=PPA/V???PA) considerably varied throughout lung decellularization, particularly for pressure controlled perfusion, as compared with flow-controlled perfusion. This study shows that monitoring perfusion mechanics throughout decellularization provides information relevant for optimizing the process time while ensuring that vascular pressure is kept within a safety range to preserve the organ scaffold integrity. Moreover, arterial lung pressure has more influence on behavior of vascular resistance in decellularized lungs than positive airway pressure, providing information that could be relevant for future cell repopulation by using the vascular resistance as a facilitator cell distribution throughout pulmonary circuit. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-10-07 |
dc.date.accessioned.fl_str_mv |
2018-06-21T19:41:07Z |
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info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Palma, Renata Kelly da. Estudo do comportamento da mec??nica vascular no processo de descelulariza????o pulmonar. 2015. 125 f. Tese( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/1830 |
identifier_str_mv |
Palma, Renata Kelly da. Estudo do comportamento da mec??nica vascular no processo de descelulariza????o pulmonar. 2015. 125 f. Tese( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo. |
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http://bibliotecatede.uninove.br/handle/tede/1830 |
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por |
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por |
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Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o |
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UNINOVE |
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Universidade Nove de Julho |
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