A influência dos parâmetros radiométricos na eficiência da ação antimicrobiana da PDT com o fotossensibilizador Protoporfirina IX
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/2849 |
Resumo: | Conventional antimicrobial strategies are becoming ineffective due to the resistance of pathogenic microorganisms to the drugs, triggering the need to explore treatments and alternative approaches to control these infections. This work is a controlled laboratory test, whose objective is to evaluate the efficacy of aPDT with the photosensitizer PpIX for inactivation of C. albicans as a function of the radiant photon energy. The experiments were carried out in two stages: in the first stage, the same total energy of the LEDs were maintained at the wavelengths: 630 nm, 570 nm, 520 nm and 440 nm during the 60 s, 120 s, 240 s and 480 s (The number of photons emitted by each wavelength will be different due to its energy). In the second step, the same amount and photon flux will be maintained for all wavelengths: 630 nm, 570 nm, 520 nm and 440 nm during the 240s, 480s and 960s times. (Different total energy). PpIX proved to be a very efficient photosensitizer capable of generating sufficient reactive oxygen species and inducing the death of C. albicans in a significant way. The evaluation of the results showed that the greater the flow of photons, the greater the antimicrobial action of PDT with PpIX, since it generates a greater amount of ROS, resulting in a greater efficacy in the elimination of C. albicans. In this way, when the wavelength is longer, photon energy will be lower. So the red photon has less energy than a blue photon and more photons are needed in the red spectral region to produce the same radiant energy as the blue spectral region. Inactivation of microorganisms by PDT is a promising alternative to the use of conventional fungicides and can be used both to control localized fungal infections and to eliminate pathogenic species from the environment. In conclusion, the evaluation of the results showed that the higher the number of photons, the greater the antimicrobial action of PDT with PpIX, as expected. |
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Deana, Alessandro MeloDeana, Alessandro MeloPavani, ChristianeKato, Ilka Tiemyhttp://lattes.cnpq.br/5173978214895876Dias, Elisabete Cristina2022-04-11T17:47:27Z2016-12-15Dias, Elisabete Cristina. A influência dos parâmetros radiométricos na eficiência da ação antimicrobiana da PDT com o fotossensibilizador Protoporfirina IX. 2016. 60 f. Dissertação( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo.http://bibliotecatede.uninove.br/handle/tede/2849Conventional antimicrobial strategies are becoming ineffective due to the resistance of pathogenic microorganisms to the drugs, triggering the need to explore treatments and alternative approaches to control these infections. This work is a controlled laboratory test, whose objective is to evaluate the efficacy of aPDT with the photosensitizer PpIX for inactivation of C. albicans as a function of the radiant photon energy. The experiments were carried out in two stages: in the first stage, the same total energy of the LEDs were maintained at the wavelengths: 630 nm, 570 nm, 520 nm and 440 nm during the 60 s, 120 s, 240 s and 480 s (The number of photons emitted by each wavelength will be different due to its energy). In the second step, the same amount and photon flux will be maintained for all wavelengths: 630 nm, 570 nm, 520 nm and 440 nm during the 240s, 480s and 960s times. (Different total energy). PpIX proved to be a very efficient photosensitizer capable of generating sufficient reactive oxygen species and inducing the death of C. albicans in a significant way. The evaluation of the results showed that the greater the flow of photons, the greater the antimicrobial action of PDT with PpIX, since it generates a greater amount of ROS, resulting in a greater efficacy in the elimination of C. albicans. In this way, when the wavelength is longer, photon energy will be lower. So the red photon has less energy than a blue photon and more photons are needed in the red spectral region to produce the same radiant energy as the blue spectral region. Inactivation of microorganisms by PDT is a promising alternative to the use of conventional fungicides and can be used both to control localized fungal infections and to eliminate pathogenic species from the environment. In conclusion, the evaluation of the results showed that the higher the number of photons, the greater the antimicrobial action of PDT with PpIX, as expected.As estratégias antimicrobianas convencionais estão se tornando ineficazes devido à resistência dos microrganismos patogênicos aos medicamentos, desencadeando a necessidade da exploração de tratamentos e abordagens alternativas para o controle dessas infecções. Este trabalho é um ensaio laboratorial controlado, que tem como objetivo avaliar a eficácia da aPDT com o fotossensibilizador PpIX para inativação de C. albicans em função dos parâmetros radiométricos. Os experimentos foram realizados em duas etapas: na primeira etapa, foi mantida a mesma energia total dos LED nos comprimentos de onda: 630 nm, 570 nm, 520 nm e 440 nm durante os tempos de 60 s, 120 s, 240 s e 480 s (número de fótons emitidos por cada comprimento de onda será diferente devido à sua energia). Na segunda etapa, será mantido o mesmo fluxo de fótons para todos os comprimentos de onda: 630 nm, 570 nm, 520 nm e 440 nm durante os tempos de 240 s, 48 0s e 960 s. (energia total diferente). A PpIX se mostrou um fotossensibilizador muito eficiente, capaz de gerar espécies reativas de oxigênio suficiente e induzir a morte de C. albicans de maneira significativa. A avaliação dos resultados demonstrou que quanto maior o fluxo de fótons, maior a ação antimicrobiana da PDT com PpIX, pois gera maior quantidade de EROs resultando em uma maior eficácia na eliminação da C. albicans. Dessa maneira, quando o comprimento de onda for maior, energia do fóton será menor, entretanto diferentes energias totais com o mesmo número de fótons produzem resultados similares. A inativação de microrganismos pela PDT é uma alternativa promissora à utilização de fungicidas convencionais e pode ser utilizada tanto para controlar infecções fungicas localizadas, como para eliminar espécies patogênicas do ambiente. Em conclusão, os resultados demonstraram que quanto maior o número de fótons, maior a ação antimicrobiana da PDT com PpIX.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2022-04-11T17:47:27Z No. of bitstreams: 1 Elisabete Cristina Dias.pdf: 1887448 bytes, checksum: 5346b2940a35aa2f5f5526829503a9cf (MD5)Made available in DSpace on 2022-04-11T17:47:27Z (GMT). No. of bitstreams: 1 Elisabete Cristina Dias.pdf: 1887448 bytes, checksum: 5346b2940a35aa2f5f5526829503a9cf (MD5) Previous issue date: 2016-12-15application/pdfporUniversidade Nove de JulhoPrograma de Pós-Graduação em Biofotônica Aplicada às Ciências da SaúdeUNINOVEBrasilSaúdePDTaPDTterapia fotodinâmicacandida albicansprotoporfirinaPDTaPDTphotodynamic therapycandida albicansprotoporphyrinCIENCIAS DA SAUDEA influência dos parâmetros radiométricos na eficiência da ação antimicrobiana da PDT com o fotossensibilizador Protoporfirina IXThe influence of radiometric parameters on the efficiency of the antimicrobial action of PDT with the photosensitizer Protoporphyrin IXinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALElisabete Cristina Dias.pdfElisabete Cristina Dias.pdfapplication/pdf1887448http://localhost:8080/tede/bitstream/tede/2849/2/Elisabete+Cristina+Dias.pdf5346b2940a35aa2f5f5526829503a9cfMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/2849/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/28492022-06-03 12:48:38.605oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2022-06-03T15:48:38Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
A influência dos parâmetros radiométricos na eficiência da ação antimicrobiana da PDT com o fotossensibilizador Protoporfirina IX |
dc.title.alternative.eng.fl_str_mv |
The influence of radiometric parameters on the efficiency of the antimicrobial action of PDT with the photosensitizer Protoporphyrin IX |
title |
A influência dos parâmetros radiométricos na eficiência da ação antimicrobiana da PDT com o fotossensibilizador Protoporfirina IX |
spellingShingle |
A influência dos parâmetros radiométricos na eficiência da ação antimicrobiana da PDT com o fotossensibilizador Protoporfirina IX Dias, Elisabete Cristina PDT aPDT terapia fotodinâmica candida albicans protoporfirina PDT aPDT photodynamic therapy candida albicans protoporphyrin CIENCIAS DA SAUDE |
title_short |
A influência dos parâmetros radiométricos na eficiência da ação antimicrobiana da PDT com o fotossensibilizador Protoporfirina IX |
title_full |
A influência dos parâmetros radiométricos na eficiência da ação antimicrobiana da PDT com o fotossensibilizador Protoporfirina IX |
title_fullStr |
A influência dos parâmetros radiométricos na eficiência da ação antimicrobiana da PDT com o fotossensibilizador Protoporfirina IX |
title_full_unstemmed |
A influência dos parâmetros radiométricos na eficiência da ação antimicrobiana da PDT com o fotossensibilizador Protoporfirina IX |
title_sort |
A influência dos parâmetros radiométricos na eficiência da ação antimicrobiana da PDT com o fotossensibilizador Protoporfirina IX |
author |
Dias, Elisabete Cristina |
author_facet |
Dias, Elisabete Cristina |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Deana, Alessandro Melo |
dc.contributor.referee1.fl_str_mv |
Deana, Alessandro Melo |
dc.contributor.referee2.fl_str_mv |
Pavani, Christiane |
dc.contributor.referee3.fl_str_mv |
Kato, Ilka Tiemy |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5173978214895876 |
dc.contributor.author.fl_str_mv |
Dias, Elisabete Cristina |
contributor_str_mv |
Deana, Alessandro Melo Deana, Alessandro Melo Pavani, Christiane Kato, Ilka Tiemy |
dc.subject.por.fl_str_mv |
PDT aPDT terapia fotodinâmica candida albicans protoporfirina |
topic |
PDT aPDT terapia fotodinâmica candida albicans protoporfirina PDT aPDT photodynamic therapy candida albicans protoporphyrin CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
PDT aPDT photodynamic therapy candida albicans protoporphyrin |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Conventional antimicrobial strategies are becoming ineffective due to the resistance of pathogenic microorganisms to the drugs, triggering the need to explore treatments and alternative approaches to control these infections. This work is a controlled laboratory test, whose objective is to evaluate the efficacy of aPDT with the photosensitizer PpIX for inactivation of C. albicans as a function of the radiant photon energy. The experiments were carried out in two stages: in the first stage, the same total energy of the LEDs were maintained at the wavelengths: 630 nm, 570 nm, 520 nm and 440 nm during the 60 s, 120 s, 240 s and 480 s (The number of photons emitted by each wavelength will be different due to its energy). In the second step, the same amount and photon flux will be maintained for all wavelengths: 630 nm, 570 nm, 520 nm and 440 nm during the 240s, 480s and 960s times. (Different total energy). PpIX proved to be a very efficient photosensitizer capable of generating sufficient reactive oxygen species and inducing the death of C. albicans in a significant way. The evaluation of the results showed that the greater the flow of photons, the greater the antimicrobial action of PDT with PpIX, since it generates a greater amount of ROS, resulting in a greater efficacy in the elimination of C. albicans. In this way, when the wavelength is longer, photon energy will be lower. So the red photon has less energy than a blue photon and more photons are needed in the red spectral region to produce the same radiant energy as the blue spectral region. Inactivation of microorganisms by PDT is a promising alternative to the use of conventional fungicides and can be used both to control localized fungal infections and to eliminate pathogenic species from the environment. In conclusion, the evaluation of the results showed that the higher the number of photons, the greater the antimicrobial action of PDT with PpIX, as expected. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-12-15 |
dc.date.accessioned.fl_str_mv |
2022-04-11T17:47:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Dias, Elisabete Cristina. A influência dos parâmetros radiométricos na eficiência da ação antimicrobiana da PDT com o fotossensibilizador Protoporfirina IX. 2016. 60 f. Dissertação( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/2849 |
identifier_str_mv |
Dias, Elisabete Cristina. A influência dos parâmetros radiométricos na eficiência da ação antimicrobiana da PDT com o fotossensibilizador Protoporfirina IX. 2016. 60 f. Dissertação( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo. |
url |
http://bibliotecatede.uninove.br/handle/tede/2849 |
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por |
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