An??lise da a????o do laser de baixa pot??ncia na cicatriza????o de feridas em ratos idosos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/1337 |
Resumo: | The optimization of time for the healing of a wound is very important, in view of the consequences it may cause, and this optimization becomes even more relevant in individuals with advanced age, where the repair is slower. The objective of this study was to evaluate the effect of low power laser on healing in an experimental model of skin wounds in aged rats. The skin wound was produced on the back using a "punch" 8mm diameter. 45 rats were males, of which 15 young people (?? 30 days) and 30 elderly (?? 500 days), divided into three groups, control young, control age and treated which underwent skin wound, and the group old treated received low-level laser treatment (30 mW, power density of 1.07 W / cm2), the area 0,028cm2 beam and wavelength ??660nm, active means of InGaAlP. Analyses were conducted to check the effects of laser on the expression of collagen type I and III, MMP-3, MMP-9, TIMP-2 and VEGF by immunohistochemical staining, IL-6 expression by RT-PCR and CINC-1 expression by ELISA. The results show that the laser decreased IL-6 and IL-8, MMP-3 and MMP-9, increased levels of TIMP-2 and VEGF, and favored collagen I and III deposition at the three time points (3, 7 and 14 days). We can conclude that laser therapy with the parameters used was effective in the treatment of skin wounds in aged animals at different stages of the process of tissue repair by modulating the expression of mediators of tissue repair and collagen deposition. |
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Carvalho, Paulo de Tarso Camillo dehttp://lattes.cnpq.br/2378784910179477Carvalho, Paulo de Tarso Camillo dehttp://lattes.cnpq.br/2378784910179477Uchiyama, Tatiana de Sousa da Cunhahttp://lattes.cnpq.br/6737487161341934Frigo, L??ciohttp://lattes.cnpq.br/8191788652250529Leal Junior, Ernesto Cesar Pintohttp://lattes.cnpq.br/8166626486201116Marcos, Rodrigo Labathttp://lattes.cnpq.br/2964112163504080http://lattes.cnpq.br/5645701615592011Fi??rio, Franciane Barbieri2016-05-25T14:13:04Z2014-12-18Fi??rio, Franciane Barbieri. An??lise da a????o do laser de baixa pot??ncia na cicatriza????o de feridas em ratos idosos. 2014. 98 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1337The optimization of time for the healing of a wound is very important, in view of the consequences it may cause, and this optimization becomes even more relevant in individuals with advanced age, where the repair is slower. The objective of this study was to evaluate the effect of low power laser on healing in an experimental model of skin wounds in aged rats. The skin wound was produced on the back using a "punch" 8mm diameter. 45 rats were males, of which 15 young people (?? 30 days) and 30 elderly (?? 500 days), divided into three groups, control young, control age and treated which underwent skin wound, and the group old treated received low-level laser treatment (30 mW, power density of 1.07 W / cm2), the area 0,028cm2 beam and wavelength ??660nm, active means of InGaAlP. Analyses were conducted to check the effects of laser on the expression of collagen type I and III, MMP-3, MMP-9, TIMP-2 and VEGF by immunohistochemical staining, IL-6 expression by RT-PCR and CINC-1 expression by ELISA. The results show that the laser decreased IL-6 and IL-8, MMP-3 and MMP-9, increased levels of TIMP-2 and VEGF, and favored collagen I and III deposition at the three time points (3, 7 and 14 days). We can conclude that laser therapy with the parameters used was effective in the treatment of skin wounds in aged animals at different stages of the process of tissue repair by modulating the expression of mediators of tissue repair and collagen deposition.A otimiza????o do tempo para a cicatriza????o de uma ferida ?? de suma import??ncia, tendo em vista as sequelas que ela pode ocasionar e, esta otimiza????o se torna ainda mais relevante no indiv??duo com idade avan??ada, onde o reparo ?? mais lento. O objetivo deste estudo foi avaliar o efeito do laser de baixa pot??ncia na cicatriza????o em um modelo experimental de feridas cut??neas em ratos idosos. A ferida cut??nea foi produzida no dorso do animal utilizando um "punch" de 8mm de di??metro. Foram utilizados 45 ratos, machos, dos quais 15 jovens (?? 30 dias) e 30 idosos (??500 dias), distribu??dos em tr??s grupos experimentais, controle jovem, controle idoso e tratado idoso, que foram submetidos ?? ferida cut??nea, e o grupo idoso tratado recebeu tratamento com laser de baixa pot??ncia (30 mW, densidade de pot??ncia de 1,07 W/cm2), ??rea do feixe de 0,028cm2 e comprimento de onda de ??660nm, meio ativo de InGaAlP. Foram realizadas an??lises para verificar os efeitos do laser sobre a express??o de col??geno tipo I e III, MMP-3, MMP-9, TIMP-2 e VEGF atrav??s de imunoistoqu??mica, express??o de IL-6 atrav??s de RT-PCR e express??o de CINC-1 atrav??s de ELISA. Os resultados mostram que o laser diminuiu os n??veis de IL-6 e IL-8, de MMP-3 e MMP-9, aumentou os n??veis de TIMP-2 e VEGF e favoreceu a deposi????o de col??geno I e III nos tr??s tempos experimentais (3, 7 e 14 dias). Podemos concluir que a laserterapia com os par??metros utilizados foi eficaz no tratamento de feridas cut??neas em animais idosos, em diferentes fases do processo de repara????o tecidual, modulando a express??o de mediadores do processo de reparo tecidual e deposi????o de col??geno.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2016-05-25T14:13:04Z No. of bitstreams: 1 Franciane Barbieri Fiorio.pdf: 1211751 bytes, checksum: 1c9c3d2ae4759d7517b8eb73fab2b12a (MD5)Made available in DSpace on 2016-05-25T14:13:04Z (GMT). No. of bitstreams: 1 Franciane Barbieri Fiorio.pdf: 1211751 bytes, checksum: 1c9c3d2ae4759d7517b8eb73fab2b12a (MD5) Previous issue date: 2014-12-18application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBrasilSa??decicatriza????o de feridaslaser de baixa pot??nciaidososwound healinglow-power laseragedCIENCIAS DA SAUDEAn??lise da a????o do laser de baixa pot??ncia na cicatriza????o de feridas em ratos idososLow level laser therapy in wound-repair process in aged ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALFranciane Barbieri Fiorio.pdfFranciane Barbieri Fiorio.pdfapplication/pdf1211751http://localhost:8080/tede/bitstream/tede/1337/2/Franciane+Barbieri+Fiorio.pdf1c9c3d2ae4759d7517b8eb73fab2b12aMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/1337/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/13372017-05-26 11:48:18.359oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2017-05-26T14:48:18Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
An??lise da a????o do laser de baixa pot??ncia na cicatriza????o de feridas em ratos idosos |
dc.title.alternative.eng.fl_str_mv |
Low level laser therapy in wound-repair process in aged rats |
title |
An??lise da a????o do laser de baixa pot??ncia na cicatriza????o de feridas em ratos idosos |
spellingShingle |
An??lise da a????o do laser de baixa pot??ncia na cicatriza????o de feridas em ratos idosos Fi??rio, Franciane Barbieri cicatriza????o de feridas laser de baixa pot??ncia idosos wound healing low-power laser aged CIENCIAS DA SAUDE |
title_short |
An??lise da a????o do laser de baixa pot??ncia na cicatriza????o de feridas em ratos idosos |
title_full |
An??lise da a????o do laser de baixa pot??ncia na cicatriza????o de feridas em ratos idosos |
title_fullStr |
An??lise da a????o do laser de baixa pot??ncia na cicatriza????o de feridas em ratos idosos |
title_full_unstemmed |
An??lise da a????o do laser de baixa pot??ncia na cicatriza????o de feridas em ratos idosos |
title_sort |
An??lise da a????o do laser de baixa pot??ncia na cicatriza????o de feridas em ratos idosos |
author |
Fi??rio, Franciane Barbieri |
author_facet |
Fi??rio, Franciane Barbieri |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Carvalho, Paulo de Tarso Camillo de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2378784910179477 |
dc.contributor.referee1.fl_str_mv |
Carvalho, Paulo de Tarso Camillo de |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/2378784910179477 |
dc.contributor.referee2.fl_str_mv |
Uchiyama, Tatiana de Sousa da Cunha |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/6737487161341934 |
dc.contributor.referee3.fl_str_mv |
Frigo, L??cio |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/8191788652250529 |
dc.contributor.referee4.fl_str_mv |
Leal Junior, Ernesto Cesar Pinto |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/8166626486201116 |
dc.contributor.referee5.fl_str_mv |
Marcos, Rodrigo Labat |
dc.contributor.referee5Lattes.fl_str_mv |
http://lattes.cnpq.br/2964112163504080 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5645701615592011 |
dc.contributor.author.fl_str_mv |
Fi??rio, Franciane Barbieri |
contributor_str_mv |
Carvalho, Paulo de Tarso Camillo de Carvalho, Paulo de Tarso Camillo de Uchiyama, Tatiana de Sousa da Cunha Frigo, L??cio Leal Junior, Ernesto Cesar Pinto Marcos, Rodrigo Labat |
dc.subject.por.fl_str_mv |
cicatriza????o de feridas laser de baixa pot??ncia idosos |
topic |
cicatriza????o de feridas laser de baixa pot??ncia idosos wound healing low-power laser aged CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
wound healing low-power laser aged |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
The optimization of time for the healing of a wound is very important, in view of the consequences it may cause, and this optimization becomes even more relevant in individuals with advanced age, where the repair is slower. The objective of this study was to evaluate the effect of low power laser on healing in an experimental model of skin wounds in aged rats. The skin wound was produced on the back using a "punch" 8mm diameter. 45 rats were males, of which 15 young people (?? 30 days) and 30 elderly (?? 500 days), divided into three groups, control young, control age and treated which underwent skin wound, and the group old treated received low-level laser treatment (30 mW, power density of 1.07 W / cm2), the area 0,028cm2 beam and wavelength ??660nm, active means of InGaAlP. Analyses were conducted to check the effects of laser on the expression of collagen type I and III, MMP-3, MMP-9, TIMP-2 and VEGF by immunohistochemical staining, IL-6 expression by RT-PCR and CINC-1 expression by ELISA. The results show that the laser decreased IL-6 and IL-8, MMP-3 and MMP-9, increased levels of TIMP-2 and VEGF, and favored collagen I and III deposition at the three time points (3, 7 and 14 days). We can conclude that laser therapy with the parameters used was effective in the treatment of skin wounds in aged animals at different stages of the process of tissue repair by modulating the expression of mediators of tissue repair and collagen deposition. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-12-18 |
dc.date.accessioned.fl_str_mv |
2016-05-25T14:13:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Fi??rio, Franciane Barbieri. An??lise da a????o do laser de baixa pot??ncia na cicatriza????o de feridas em ratos idosos. 2014. 98 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/1337 |
identifier_str_mv |
Fi??rio, Franciane Barbieri. An??lise da a????o do laser de baixa pot??ncia na cicatriza????o de feridas em ratos idosos. 2014. 98 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo. |
url |
http://bibliotecatede.uninove.br/handle/tede/1337 |
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por |
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por |
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Universidade Nove de Julho |
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Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o |
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UNINOVE |
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Brasil |
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Sa??de |
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Universidade Nove de Julho |
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