Potencial dos fatores de risco associados aos marcadores biomoleculares RNAm IDO E RNAm CDKN2A/p16 na predi????o das les??es precursoras do c??ncer de colo uterino

• Autor(a) principal: Saffi Junior, Mario Cezar
• Data de Publicação: 2015
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da Uninove
• Texto Completo: http://bibliotecatede.uninove.br/handle/tede/1300
• topic: neoplasias do colo do ??tero; les??es pr??-neopl??sicas; HPV; citologia; fatores de risco; colposcopia; neoplasia intraepitelial cervical; fator prognostico; genes p16; indoleamine 2,3-dioxygenase; IDO; cervical cancer; pre-neoplastic lesions; HPV; cytology; risk factors; colposcopy; cervical intraepithelial neoplasia; prognostic factor; p16 genes; CDKN2A; indoleamine 2,3-dioxygenase; IDO; CIENCIAS DA SAUDE

The cervical cancer is the first cancer of the female genital tract in Brazil and HPV is essential factor for carcinogenesis. The Brazilian program tracking proposes conventional cervical cytology as the primary method to detect cervical cancer, despite its low sensitivity. Risk factors associated with the spread of HPV are despised and not rely on a biomolecular tool that can increase the program offered by the Ministry of Health. The aim of this study was to determine whether the risk factors for cervical cancer may contribute to the conventional cervical cytology to increase diagnostic sensitivity and assess whether the mRNA indoleamine 2,3 dioxygenase (IDO) and mRNA CDKN2A / p16 may increase the diagnostic yield of this neoplasm. The logistic regression analysis was based on clinical variables (risk factors), cytological and biomolecular to seek an association with pathological results. The proportion of explained variance (PVE) for each variable studied was calculated by the formula omega, whereas the sensitivity, specificity, positive predictive value and negative predictive value were calculated by the formulas of Galen and Gambino. We conclude that oral contraceptive showed greater predictive power of high-grade lesions compared to other risk factors, and that both the IDO mRNA as CDKN2A mRNA / p16 may help screening of cervical cancer, either when used alone, or in conjunction with conventional cervical cytology, increasing their sensitivity and maintaining a considerable specificity.