Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/1802 |
Resumo: | The activation, proliferation and migration of myogenic precursor cells are essential for muscle regeneration after injury, orchestrated by cells and local components, mainly inflammatory cells, especially macrophages. These are identified as a target for the treatment of muscle injuries. Laser therapy has shown good results in treatment of injuries and the ability to accelerate the migration of various cell types, but there are no reports on their effect on macrophage products influencing the migration of myogenic precursor cells. The aim of the study was to evaluate the effect of low level laser (LLL) on migration of myoblasts cultured with macrophage culture supernatants of different phenotypes. Therefore, C2C12 cells were cultured with supernatants from cultures of macrophages (J774) treated with LPS and IFN-?? (for the activation phenotype M1), IL-4 (M2a phenotype) and IL-10 and dexamethasone (M2c phenotype) and LLL irradiated at wavelengths of 660nm and 780nm (70mW; 17,5J / cm2; 14.3 s; 0,8J). Supernatants from macrophage cultures were harvested 24h after irradiation and transferred to myoblast cultures. Migration was assessed using the scratch assay and the results statistically analyzed. Myoblasts cultured with phenotype macrophage supernatants M2c and irradiated with LBP (660nm) showed higher migration capability that cultured with supernatants of M2C phenotype of macrophages after 12 hours of cultivation. There was no difference between the other groups. The LLL was able to modulate the migration of myoblasts C2C12 when M2c supernatants of macrophage phenotype |
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Fernandes, Kristianne Porta SantosFerrari, Raquel Agnelli MesquitaFernandes, Kristianne Porta SantosFran??a, Cristiane MirandaIemma, Monica Rosas da Costahttp://lattes.cnpq.br/2520841630025710Batista, ??rika C??ssia Barroso2018-06-18T22:38:09Z2015-12-16Batista, ??rika C??ssia Barroso. Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia. 2015. 48 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1802The activation, proliferation and migration of myogenic precursor cells are essential for muscle regeneration after injury, orchestrated by cells and local components, mainly inflammatory cells, especially macrophages. These are identified as a target for the treatment of muscle injuries. Laser therapy has shown good results in treatment of injuries and the ability to accelerate the migration of various cell types, but there are no reports on their effect on macrophage products influencing the migration of myogenic precursor cells. The aim of the study was to evaluate the effect of low level laser (LLL) on migration of myoblasts cultured with macrophage culture supernatants of different phenotypes. Therefore, C2C12 cells were cultured with supernatants from cultures of macrophages (J774) treated with LPS and IFN-?? (for the activation phenotype M1), IL-4 (M2a phenotype) and IL-10 and dexamethasone (M2c phenotype) and LLL irradiated at wavelengths of 660nm and 780nm (70mW; 17,5J / cm2; 14.3 s; 0,8J). Supernatants from macrophage cultures were harvested 24h after irradiation and transferred to myoblast cultures. Migration was assessed using the scratch assay and the results statistically analyzed. Myoblasts cultured with phenotype macrophage supernatants M2c and irradiated with LBP (660nm) showed higher migration capability that cultured with supernatants of M2C phenotype of macrophages after 12 hours of cultivation. There was no difference between the other groups. The LLL was able to modulate the migration of myoblasts C2C12 when M2c supernatants of macrophage phenotypeA ativa????o, prolifera????o e migra????o das c??lulas precursoras miog??nicas s??o essenciais na regenera????o muscular ap??s les??es, orquestrados pelas c??lulas e componentes locais, principalmente pelas c??lulas inflamat??rias, em especial os macr??fagos. Estes s??o apontadas como alvo para o tratamento das les??es musculares. A laserterapia tem demonstrado bons resultados no tratamento destas les??es e na capacidade de acelerar a migra????o de v??rios tipos celulares, mas n??o existem relatos sobre seu efeito nos produtos de macr??fagos que influenciam a migra????o de c??lulas precursoras miog??nicas. O objetivo do estudo foi avaliar o efeito do laser de baixa pot??ncia (LBP) sobre a migra????o de mioblastos cultivadas com sobrenadantes de culturas de macr??fagos de diferentes fen??tipos. Para tanto, as c??lulas C2C12 foram cultivadas com sobrenadantes de culturas de macr??fagos (J774) tratadas com LPS e IFN- ?? (ativa????o para o fen??tipo M1), IL-4 (fen??tipo M2a) e IL-10 e dexametasona (fen??tipo M2c) e irradiadas com LBP nos comprimentos de onda de 660nm e 780nm (70mW; 17,5J/cm2; 14,3 s; 0,8J). Os sobrenadantes das culturas de macr??fagos foram colhidos 24h ap??s as irradia????es e transferidos para culturas de mioblastos. A migra????o foi avaliada por meio do ensaio de ferida e os resultados submetidos ?? an??lise estat??stica. Ap??s 12h de cultivo, os mioblastos cultivados com sobrenadantes de macr??fagos de fen??tipo M2c e irradiados com LBP (660nm) mostraram maior capacidade de migra????o que os cultivados com sobrenadantes de macr??fagos de fen??tipo M2c n??o irradiados. N??o houve diferen??a entre os demais grupos. O LBP foi capaz de modular a migra????o de mioblastos C2C12 quando cultivados com sobrenadantes de macr??fagos de fen??tipo M2c.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2018-06-18T22:38:09Z No. of bitstreams: 1 Erika Cassia Barroso Batista.pdf: 1153764 bytes, checksum: d2f3d36a962ecb178963820d00e754fa (MD5)Made available in DSpace on 2018-06-18T22:38:09Z (GMT). No. of bitstreams: 1 Erika Cassia Barroso Batista.pdf: 1153764 bytes, checksum: d2f3d36a962ecb178963820d00e754fa (MD5) Previous issue date: 2015-12-16application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBrasilSa??demigra????oc??lulas precursoras miog??nicasmacr??fagoslaser de baixa pot??nciamigrationmyogenic precursor cellsmacrophageslow level laserCIENCIAS DA SAUDEMigra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??nciaMigration of myogenic precursor cells under the influence of macrophage supernatants irradiated with low level laserinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALErika Cassia Barroso Batista.pdfErika Cassia Barroso Batista.pdfapplication/pdf1153764http://localhost:8080/tede/bitstream/tede/1802/2/Erika+Cassia+Barroso+Batista.pdfd2f3d36a962ecb178963820d00e754faMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/1802/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/18022018-06-18 19:38:09.337oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-18T22:38:09Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia |
dc.title.alternative.eng.fl_str_mv |
Migration of myogenic precursor cells under the influence of macrophage supernatants irradiated with low level laser |
title |
Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia |
spellingShingle |
Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia Batista, ??rika C??ssia Barroso migra????o c??lulas precursoras miog??nicas macr??fagos laser de baixa pot??ncia migration myogenic precursor cells macrophages low level laser CIENCIAS DA SAUDE |
title_short |
Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia |
title_full |
Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia |
title_fullStr |
Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia |
title_full_unstemmed |
Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia |
title_sort |
Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia |
author |
Batista, ??rika C??ssia Barroso |
author_facet |
Batista, ??rika C??ssia Barroso |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Fernandes, Kristianne Porta Santos |
dc.contributor.advisor-co1.fl_str_mv |
Ferrari, Raquel Agnelli Mesquita |
dc.contributor.referee1.fl_str_mv |
Fernandes, Kristianne Porta Santos |
dc.contributor.referee2.fl_str_mv |
Fran??a, Cristiane Miranda |
dc.contributor.referee3.fl_str_mv |
Iemma, Monica Rosas da Costa |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2520841630025710 |
dc.contributor.author.fl_str_mv |
Batista, ??rika C??ssia Barroso |
contributor_str_mv |
Fernandes, Kristianne Porta Santos Ferrari, Raquel Agnelli Mesquita Fernandes, Kristianne Porta Santos Fran??a, Cristiane Miranda Iemma, Monica Rosas da Costa |
dc.subject.por.fl_str_mv |
migra????o c??lulas precursoras miog??nicas macr??fagos laser de baixa pot??ncia |
topic |
migra????o c??lulas precursoras miog??nicas macr??fagos laser de baixa pot??ncia migration myogenic precursor cells macrophages low level laser CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
migration myogenic precursor cells macrophages low level laser |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
The activation, proliferation and migration of myogenic precursor cells are essential for muscle regeneration after injury, orchestrated by cells and local components, mainly inflammatory cells, especially macrophages. These are identified as a target for the treatment of muscle injuries. Laser therapy has shown good results in treatment of injuries and the ability to accelerate the migration of various cell types, but there are no reports on their effect on macrophage products influencing the migration of myogenic precursor cells. The aim of the study was to evaluate the effect of low level laser (LLL) on migration of myoblasts cultured with macrophage culture supernatants of different phenotypes. Therefore, C2C12 cells were cultured with supernatants from cultures of macrophages (J774) treated with LPS and IFN-?? (for the activation phenotype M1), IL-4 (M2a phenotype) and IL-10 and dexamethasone (M2c phenotype) and LLL irradiated at wavelengths of 660nm and 780nm (70mW; 17,5J / cm2; 14.3 s; 0,8J). Supernatants from macrophage cultures were harvested 24h after irradiation and transferred to myoblast cultures. Migration was assessed using the scratch assay and the results statistically analyzed. Myoblasts cultured with phenotype macrophage supernatants M2c and irradiated with LBP (660nm) showed higher migration capability that cultured with supernatants of M2C phenotype of macrophages after 12 hours of cultivation. There was no difference between the other groups. The LLL was able to modulate the migration of myoblasts C2C12 when M2c supernatants of macrophage phenotype |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-12-16 |
dc.date.accessioned.fl_str_mv |
2018-06-18T22:38:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Batista, ??rika C??ssia Barroso. Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia. 2015. 48 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/1802 |
identifier_str_mv |
Batista, ??rika C??ssia Barroso. Migra????o de c??lulas precursoras miog??nicas sob a influ??ncia de sobrenadantes de macr??fagos irradiados com laser de baixa pot??ncia. 2015. 48 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo. |
url |
http://bibliotecatede.uninove.br/handle/tede/1802 |
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por |
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por |
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Universidade Nove de Julho |
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Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o |
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UNINOVE |
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