Efeito do LED sobre a viabilidade de mioblastos cultivados na presen??a ou n??o de extrato de Melaleuca armillaris

Detalhes bibliográficos
Autor(a) principal: Cardoso, Vinicius de Oliveira
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da Uninove
Texto Completo: http://bibliotecatede.uninove.br/handle/tede/1787
Resumo: The skeletal muscle cells are constant target for damage the exercise, direct trauma, inflammatory processes, among others. In order to contain or reset the loss of muscle mass, begins a highly organized repair process with activation, proliferation and differentiation of satellite cells. The use of phototherapy resources that can promote faster and efficient repair as the Low Level Laser Therapy (LLLT) has shown good results in the regeneration of skeletal muscle. The use of herbal medicines has also been described in the literature and has shown promising results with regard to tissue repair. The objective of the study was to evaluate the effects of treatment with LED on cell viability, Nitric Oxide (NO) synthesis and total protein dosage in C2C12 muscle cells cultured with Melaleuca armillaris extract in differentiation medium. We analyzed eight experimental groups: (1) Control C2C12; (2) LED 0,2J; (3) LED 2,0J; (4) LED 4,0J; (5) 1% Melaleuca; (6) Melaleuca 1% + LED 0,2J; (7) Melaleuca 1% + LED 2,0J; (8) Melaleuca 1% + LED 4,0J. Cells received Melaleuca extract at a concentration of 1.0% (v/v) and were irradiated with infrared LED (850nm, 30mW power for 7, 67 and 134 seconds at full power 0,2J, and 2,0J 4,0J, respectively) according to the group. Cell viability was assessed by MTT method, the synthesis of NO by the Griess method and total protein determination was performed using the Bradford method after periods of 1, 2 and 3 days of incubation. The results showed that cell viability increased TLED the three periods energies and tested without changing the total concentration of protein and NO. Since the herbal remedy promoted a significant reduction in cell viability compared to control small a reduction in the dosage of NO after 24 hours and no change in the protein assay. However, the combined use of the two resources, the energy value of 0.2 and showed viability 2,0J, NO and protein similar to the group treated only with 1% Melaleuca a tendency to increase in these levels in the group irradiated with 2,0J energy. However, the group associated 4,0J showed a growth recovery in cell viability in the three periods analyzed and behavior similar to nitric oxide and total protein compared to the control. In conclusion, the TLED positively modulated the proliferation of C2C12 cells without deterioration in nitric oxide synthesis and total protein, while incubation with associated or not to TLED Melaleuca needs more specific analyzes to elucidate their contribution in this context.
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spelling Ferrari, Raquel Agnelli MesquitaFerrari, Raquel Agnelli MesquitaSilva, Carlos Alberto daFran??a, Cristiane Mirandahttp://lattes.cnpq.br/7933248928885530Cardoso, Vinicius de Oliveira2018-06-15T14:58:15Z2015-02-27Cardoso, Vinicius de Oliveira. Efeito do LED sobre a viabilidade de mioblastos cultivados na presen??a ou n??o de extrato de Melaleuca armillaris. 2015. 52 f. Disserta????o( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1787The skeletal muscle cells are constant target for damage the exercise, direct trauma, inflammatory processes, among others. In order to contain or reset the loss of muscle mass, begins a highly organized repair process with activation, proliferation and differentiation of satellite cells. The use of phototherapy resources that can promote faster and efficient repair as the Low Level Laser Therapy (LLLT) has shown good results in the regeneration of skeletal muscle. The use of herbal medicines has also been described in the literature and has shown promising results with regard to tissue repair. The objective of the study was to evaluate the effects of treatment with LED on cell viability, Nitric Oxide (NO) synthesis and total protein dosage in C2C12 muscle cells cultured with Melaleuca armillaris extract in differentiation medium. We analyzed eight experimental groups: (1) Control C2C12; (2) LED 0,2J; (3) LED 2,0J; (4) LED 4,0J; (5) 1% Melaleuca; (6) Melaleuca 1% + LED 0,2J; (7) Melaleuca 1% + LED 2,0J; (8) Melaleuca 1% + LED 4,0J. Cells received Melaleuca extract at a concentration of 1.0% (v/v) and were irradiated with infrared LED (850nm, 30mW power for 7, 67 and 134 seconds at full power 0,2J, and 2,0J 4,0J, respectively) according to the group. Cell viability was assessed by MTT method, the synthesis of NO by the Griess method and total protein determination was performed using the Bradford method after periods of 1, 2 and 3 days of incubation. The results showed that cell viability increased TLED the three periods energies and tested without changing the total concentration of protein and NO. Since the herbal remedy promoted a significant reduction in cell viability compared to control small a reduction in the dosage of NO after 24 hours and no change in the protein assay. However, the combined use of the two resources, the energy value of 0.2 and showed viability 2,0J, NO and protein similar to the group treated only with 1% Melaleuca a tendency to increase in these levels in the group irradiated with 2,0J energy. However, the group associated 4,0J showed a growth recovery in cell viability in the three periods analyzed and behavior similar to nitric oxide and total protein compared to the control. In conclusion, the TLED positively modulated the proliferation of C2C12 cells without deterioration in nitric oxide synthesis and total protein, while incubation with associated or not to TLED Melaleuca needs more specific analyzes to elucidate their contribution in this context.As c??lulas do m??sculo esquel??tico sofrem constantes les??es provocadas por exerc??cio, traumas diretos, processos inflamat??rios entre outros. A fim de conter ou repor a perda de massa muscular, inicia-se um processo de reparo altamente organizado com ativa????o, prolifera????o e diferencia????o das c??lulas sat??lites. A utiliza????o de recursos fototer??picos que possam promover um reparo mais r??pido e eficiente como o Laser de Baixa Pot??ncia (LBP) tem apresentado bons resultados na regenera????o do m??sculo esquel??tico. O uso de fitoter??picos tamb??m tem sido descrito na literatura e tem evidenciado resultados promissores no que diz respeito ao reparo tecidual. Assim, o objetivo do estudo foi avaliar os efeitos do tratamento com LED sobre a viabilidade celular, s??ntese de ??xido N??trico (NO) e dosagem total de prote??nas em c??lulas musculares C2C12 cultivadas com extrato de Melaleuca armillaris em meio de diferencia????o. Foram analisados 8 grupos experimentais, sendo: (1) Controle C2C12; (2) LED 0,2J; (3) LED 2,0J; (4) LED 4,0J; (5) Melaleuca 1%; (6) Melaleuca 1%+LED 0,2J; (7) Melaleuca 1%+LED 2,0J; (8) Melaleuca 1%+LED 4,0J. As c??lulas receberam extrato de Melaleuca na concentra????o de 1,0% (v/v) e foram irradiadas com LED Infravermelho (850nm, pot??ncia de 30mW) por 7, 67 e 134 segundos, com energia total de 0,2J, 2,0J e 4,0J, respectivamente de acordo com o grupo. A viabilidade celular foi avaliada pelo m??todo de MTT, a s??ntese de NO pelo m??todo de Griess e a dosagem total de prote??nas foi feita utilizando o m??todo de Bradford ap??s os per??odos de 1, 2 e 3 dias de incuba????o. Os resultados demonstraram que a TLED aumentou a viabilidade celular nas tr??s energias e per??odos testados sem alterar a concentra????o de prote??na total e NO. J?? o recurso fitoter??pico promoveu uma grande redu????o da viabilidade celular comparada ao controle, pequena redu????o na dosagem de NO ap??s 24h e nenhuma altera????o na dosagem de prote??na. Contudo, o uso associado dos dois recursos, na energia de 0,2 e 2,0J apresentaram valor de viabilidade, NO e prote??na semelhante ao grupo tratado somente com Melaleuca 1% com uma tend??ncia no aumento destes n??veis no grupo irradiado com 2,0J de energia. Entretanto, o grupo 4,0J associado evidenciou uma retomada de crescimento na viabilidade celular nos tr??s per??odos analisados e comportamento semelhante para ??xido n??trico e prote??na total comparados ao controle. Em conclus??o, o TLED modulou positivamente a prolifera????o das c??lulas C2C12 sem apresentar altera????es na s??ntese de ??xido n??trico e prote??na total, enquanto que a incuba????o com Melaleuca associada ou n??o ao TLED necessita de an??lises mais espec??ficas para elucidar sua contribui????o neste contexto.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2018-06-15T14:58:15Z No. of bitstreams: 1 Vinicius de Oliveira Cardoso.pdf: 848877 bytes, checksum: fe763aa5d3e08d692e2c53a7d7bb14cb (MD5)Made available in DSpace on 2018-06-15T14:58:15Z (GMT). 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dc.title.por.fl_str_mv Efeito do LED sobre a viabilidade de mioblastos cultivados na presen??a ou n??o de extrato de Melaleuca armillaris
title Efeito do LED sobre a viabilidade de mioblastos cultivados na presen??a ou n??o de extrato de Melaleuca armillaris
spellingShingle Efeito do LED sobre a viabilidade de mioblastos cultivados na presen??a ou n??o de extrato de Melaleuca armillaris
Cardoso, Vinicius de Oliveira
C2C12
LED
Melaleuca armillaris
viabilidade
mioblasto
??xido n??trico
C2C12
LED
Melaleuca armillaris
viability
myoblast
nitric oxide
CIENCIAS DA SAUDE
title_short Efeito do LED sobre a viabilidade de mioblastos cultivados na presen??a ou n??o de extrato de Melaleuca armillaris
title_full Efeito do LED sobre a viabilidade de mioblastos cultivados na presen??a ou n??o de extrato de Melaleuca armillaris
title_fullStr Efeito do LED sobre a viabilidade de mioblastos cultivados na presen??a ou n??o de extrato de Melaleuca armillaris
title_full_unstemmed Efeito do LED sobre a viabilidade de mioblastos cultivados na presen??a ou n??o de extrato de Melaleuca armillaris
title_sort Efeito do LED sobre a viabilidade de mioblastos cultivados na presen??a ou n??o de extrato de Melaleuca armillaris
author Cardoso, Vinicius de Oliveira
author_facet Cardoso, Vinicius de Oliveira
author_role author
dc.contributor.advisor1.fl_str_mv Ferrari, Raquel Agnelli Mesquita
dc.contributor.referee1.fl_str_mv Ferrari, Raquel Agnelli Mesquita
dc.contributor.referee2.fl_str_mv Silva, Carlos Alberto da
dc.contributor.referee3.fl_str_mv Fran??a, Cristiane Miranda
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7933248928885530
dc.contributor.author.fl_str_mv Cardoso, Vinicius de Oliveira
contributor_str_mv Ferrari, Raquel Agnelli Mesquita
Ferrari, Raquel Agnelli Mesquita
Silva, Carlos Alberto da
Fran??a, Cristiane Miranda
dc.subject.por.fl_str_mv C2C12
LED
Melaleuca armillaris
viabilidade
mioblasto
??xido n??trico
topic C2C12
LED
Melaleuca armillaris
viabilidade
mioblasto
??xido n??trico
C2C12
LED
Melaleuca armillaris
viability
myoblast
nitric oxide
CIENCIAS DA SAUDE
dc.subject.eng.fl_str_mv C2C12
LED
Melaleuca armillaris
viability
myoblast
nitric oxide
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE
description The skeletal muscle cells are constant target for damage the exercise, direct trauma, inflammatory processes, among others. In order to contain or reset the loss of muscle mass, begins a highly organized repair process with activation, proliferation and differentiation of satellite cells. The use of phototherapy resources that can promote faster and efficient repair as the Low Level Laser Therapy (LLLT) has shown good results in the regeneration of skeletal muscle. The use of herbal medicines has also been described in the literature and has shown promising results with regard to tissue repair. The objective of the study was to evaluate the effects of treatment with LED on cell viability, Nitric Oxide (NO) synthesis and total protein dosage in C2C12 muscle cells cultured with Melaleuca armillaris extract in differentiation medium. We analyzed eight experimental groups: (1) Control C2C12; (2) LED 0,2J; (3) LED 2,0J; (4) LED 4,0J; (5) 1% Melaleuca; (6) Melaleuca 1% + LED 0,2J; (7) Melaleuca 1% + LED 2,0J; (8) Melaleuca 1% + LED 4,0J. Cells received Melaleuca extract at a concentration of 1.0% (v/v) and were irradiated with infrared LED (850nm, 30mW power for 7, 67 and 134 seconds at full power 0,2J, and 2,0J 4,0J, respectively) according to the group. Cell viability was assessed by MTT method, the synthesis of NO by the Griess method and total protein determination was performed using the Bradford method after periods of 1, 2 and 3 days of incubation. The results showed that cell viability increased TLED the three periods energies and tested without changing the total concentration of protein and NO. Since the herbal remedy promoted a significant reduction in cell viability compared to control small a reduction in the dosage of NO after 24 hours and no change in the protein assay. However, the combined use of the two resources, the energy value of 0.2 and showed viability 2,0J, NO and protein similar to the group treated only with 1% Melaleuca a tendency to increase in these levels in the group irradiated with 2,0J energy. However, the group associated 4,0J showed a growth recovery in cell viability in the three periods analyzed and behavior similar to nitric oxide and total protein compared to the control. In conclusion, the TLED positively modulated the proliferation of C2C12 cells without deterioration in nitric oxide synthesis and total protein, while incubation with associated or not to TLED Melaleuca needs more specific analyzes to elucidate their contribution in this context.
publishDate 2015
dc.date.issued.fl_str_mv 2015-02-27
dc.date.accessioned.fl_str_mv 2018-06-15T14:58:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv Cardoso, Vinicius de Oliveira. Efeito do LED sobre a viabilidade de mioblastos cultivados na presen??a ou n??o de extrato de Melaleuca armillaris. 2015. 52 f. Disserta????o( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo.
dc.identifier.uri.fl_str_mv http://bibliotecatede.uninove.br/handle/tede/1787
identifier_str_mv Cardoso, Vinicius de Oliveira. Efeito do LED sobre a viabilidade de mioblastos cultivados na presen??a ou n??o de extrato de Melaleuca armillaris. 2015. 52 f. Disserta????o( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo.
url http://bibliotecatede.uninove.br/handle/tede/1787
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language por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Universidade Nove de Julho
dc.publisher.program.fl_str_mv Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de
dc.publisher.initials.fl_str_mv UNINOVE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Sa??de
publisher.none.fl_str_mv Universidade Nove de Julho
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