Efeito da fotobiomodulação na reação local induzida pelo veneno da serpente Bothrops alternatus em seus estágios juvenil e adulto

Detalhes bibliográficos
Autor(a) principal: David, Amanda Cabral
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da Uninove
Texto Completo: http://bibliotecatede.uninove.br/handle/tede/3307
Resumo: Bothrops snakes are responsible for most snakebites in Latin America. Bothropic envenoming causes both systemic and severe local effects. Furthermore, it is described that venoms from young and adult snakes have differences in their composition, which can lead to differences in the evolution of the local picture. Bothropic antivenom is the treatment of first choice, however, its effectiveness is limited, being able to neutralize only the systemic effects. It has been shown in previous studies that photobiomodulation (PBM) is promising in reducing local effects induced by bothropic snakes. The aim of this study was to expand the knowledge of the actions of low-level laser (LLL) on the local effect, after the action of the snake venom Bothrops alternatus (VBa), in its juvenile (VBaJ) and adult (VBaA) stages. For this, in vivo experiments were carried out with Balb-C mice and, in vitro with C2C12 muscle cells. For the treatment, LLL, 660 nm, 100 mW and 40 seconds of irradiation were used. In vivo, the animals were injected with VBaJ or VBaA, or only with saline, and the groups that received PBM, the treatment was conducted 30 minutes after the injection of the venoms. After 6 hours, edema, inflammatory infiltrate, myotoxicity and histology of the gastrocnemius muscle were analyzed, and after 1, 3, 6 and 24 hours, hyperalgesia was analyzed. In vitro, C2C12 cells received either VBaJ or VBaA, or culture medium alone, and were immediately irradiated with LLL. After 3 hours of the action of the venoms, cell viability, cytotoxicity, differentiation, membrane potential, proliferation, and cellular apoptosis/necrosis were evaluated. It was observed that, in vivo, VBaJ and VBaA, at the applied doses, were able to induce edema, inflammatory infiltrate, hemorrhage, to increase hyperalgesia and to cause myotoxicity and myonecrosis, at different intensities. In vitro, the two venoms also caused cytotoxicity, decreased cell differentiation, increased cell proliferation, and also induced apoptosis. Treatment with PBM, in vivo, was effective in reducing myotoxicity, leukocyte migration and hyperalgesia caused by the two venoms tested. Furthermore, it reduced the edema caused by VBaJ. In vitro, PBM induced cell differentiation and proliferation and also reduced apoptotic and necrotic cells. It can be concluded that, although both venoms (juvenile and adult) are capable to induce local reactions, there is a difference in the magnitude of these effects, both in vivo and in vitro, and treatment with PBM was shown to be beneficial in reducing these local reactions induced by the venoms of this snake.
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spelling Zamuner, Stella Reginahttp://lattes.cnpq.br/1935805744318404Zuliani, Juliana Pavanhttp://lattes.cnpq.br/9093880214338747Zamuner, Stella Reginahttp://lattes.cnpq.br/1935805744318404Dellê, Humbertohttp://lattes.cnpq.br/7435662740477057Leiva, Laura C.http://lattes.cnpq.br/9589613288491092David, Amanda Cabral2024-04-08T19:14:01Z2021-12-16David, Amanda Cabral. Efeito da fotobiomodulação na reação local induzida pelo veneno da serpente Bothrops alternatus em seus estágios juvenil e adulto. 2021. 99 f. Dissertação( Programa de Mestrado em Medicina) - Universidade Nove de Julho, São Paulo.http://bibliotecatede.uninove.br/handle/tede/3307Bothrops snakes are responsible for most snakebites in Latin America. Bothropic envenoming causes both systemic and severe local effects. Furthermore, it is described that venoms from young and adult snakes have differences in their composition, which can lead to differences in the evolution of the local picture. Bothropic antivenom is the treatment of first choice, however, its effectiveness is limited, being able to neutralize only the systemic effects. It has been shown in previous studies that photobiomodulation (PBM) is promising in reducing local effects induced by bothropic snakes. The aim of this study was to expand the knowledge of the actions of low-level laser (LLL) on the local effect, after the action of the snake venom Bothrops alternatus (VBa), in its juvenile (VBaJ) and adult (VBaA) stages. For this, in vivo experiments were carried out with Balb-C mice and, in vitro with C2C12 muscle cells. For the treatment, LLL, 660 nm, 100 mW and 40 seconds of irradiation were used. In vivo, the animals were injected with VBaJ or VBaA, or only with saline, and the groups that received PBM, the treatment was conducted 30 minutes after the injection of the venoms. After 6 hours, edema, inflammatory infiltrate, myotoxicity and histology of the gastrocnemius muscle were analyzed, and after 1, 3, 6 and 24 hours, hyperalgesia was analyzed. In vitro, C2C12 cells received either VBaJ or VBaA, or culture medium alone, and were immediately irradiated with LLL. After 3 hours of the action of the venoms, cell viability, cytotoxicity, differentiation, membrane potential, proliferation, and cellular apoptosis/necrosis were evaluated. It was observed that, in vivo, VBaJ and VBaA, at the applied doses, were able to induce edema, inflammatory infiltrate, hemorrhage, to increase hyperalgesia and to cause myotoxicity and myonecrosis, at different intensities. In vitro, the two venoms also caused cytotoxicity, decreased cell differentiation, increased cell proliferation, and also induced apoptosis. Treatment with PBM, in vivo, was effective in reducing myotoxicity, leukocyte migration and hyperalgesia caused by the two venoms tested. Furthermore, it reduced the edema caused by VBaJ. In vitro, PBM induced cell differentiation and proliferation and also reduced apoptotic and necrotic cells. It can be concluded that, although both venoms (juvenile and adult) are capable to induce local reactions, there is a difference in the magnitude of these effects, both in vivo and in vitro, and treatment with PBM was shown to be beneficial in reducing these local reactions induced by the venoms of this snake.As serpentes do gênero Bothrops são responsáveis pela maioria dos acidentes ofídicos na América Latina. O envenenamento botrópico provoca tanto efeitos sistêmicos, como efeitos locais graves. Além disso, é descrito que venenos de serpentes jovens e adultas possuem diferenças em sua composição, o que pode levar a diferenças na evolução do quadro local. O antiveneno botrópico é o tratamento de primeira escolha, entretanto, sua eficácia é limitada, sendo capaz de neutralizar somente os efeitos sistêmicos. Já foi demonstrado em estudos anteriores, que a fotobiomodulação (FBM) é promissora na redução dos efeitos locais induzidos por serpentes botrópicas. O objetivo deste estudo foi ampliar o conhecimento das ações do laser de baixa intensidade (LBI) sobre o efeito local, após ação do veneno da serpente Bothrops alternatus (VBa), em seus estágios juvenil (VBaJ) e adulto (VBaA). Para isto, foram realizados experimentos in vivo com camundongos Balb-C e, in vitro, com células musculares C2C12. Para o tratamento, foi utilizado o LBI, 660 nm, 100 mW e 40 segundos de irradiação. In vivo, os animais foram injetados com o VBaJ ou VBaA, ou somente com salina e os grupos que receberam a FBM, o tratamento foi feito após 30 minutos da injeção dos venenos. Após 6 horas, foram analisados o edema, infiltrado inflamatório, a miotoxicidade e a histologia do músculo gastrocnêmio e, após 1, 3, 6 e 24 horas, foi analisada a hiperalgesia. In vitro, as células C2C12 receberam o VBaJ ou VBaA, ou somente meio de cultura, e foram imediatamente irradiadas com o LBI. Após 3 horas da ação dos venenos, foram avaliados a viabilidade celular, a citotoxicidade, a diferenciação, o potencial de membrana, a proliferação e a apoptose/necrose celular. Foi observado que, in vivo, o VBaJ e VBaA, nas doses aplicadas, foram capazes de induzir edema, infiltrado inflamatório, hemorragia, de aumentar a hiperalgesia e de causar miotoxicidade e mionecrose, em diferentes intensidades. In vitro, os dois venenos também causaram citotoxicidade, diminuíram a diferenciação celular, aumentaram a proliferação celular e também induziram apoptose. O tratamento com a FBM, in vivo, foi eficaz em reduzir a miotoxicidade, a migração leucocitária e a hiperalgesia causadas pelos dois venenos testados. Ainda, reduziu o edema causado pelo VBaJ. Já in vitro, a FBM induziu a diferenciação e proliferação celular e também reduziu as células apoptóticas e necróticas. Pode-se concluir que, embora ambos os venenos (juvenil e adulto) sejam capazes de causar reações locais, existe diferença na magnitude desses efeitos, tanto in vivo como in vitro, e o tratamento com a FBM se mostrou benéfico em reduzir essas reações locais induzidas pelos venenos desta serpente.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2024-04-08T19:14:01Z No. of bitstreams: 1 Amanda Cabral David.pdf: 2094031 bytes, checksum: d51d3a020366e92f2db0caf0542bb316 (MD5)Made available in DSpace on 2024-04-08T19:14:01Z (GMT). No. of bitstreams: 1 Amanda Cabral David.pdf: 2094031 bytes, checksum: d51d3a020366e92f2db0caf0542bb316 (MD5) Previous issue date: 2021-12-16application/pdfporUniversidade Nove de JulhoPrograma de Mestrado em MedicinaUNINOVEBrasilSaúdeBothrops alternatusjuvenil e adultofotobiomodulaçãomiotoxicidadecitotoxicidadeveneno botrópicoBothrops alternatusjuvenile and adultphotobiomodulationmyotoxicitycytotoxicitybothropic venomCIENCIAS DA SAUDEEfeito da fotobiomodulação na reação local induzida pelo veneno da serpente Bothrops alternatus em seus estágios juvenil e adultoEffect of photobiomodulation on local reaction induced by the venon of Bothrops alternatus on its juvenile and adult stagesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALAmanda Cabral David.pdfAmanda Cabral David.pdfapplication/pdf2094031http://localhost:8080/tede/bitstream/tede/3307/2/Amanda+Cabral+David.pdfd51d3a020366e92f2db0caf0542bb316MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/3307/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/33072024-04-08 16:14:01.156oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2024-04-08T19:14:01Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false
dc.title.por.fl_str_mv Efeito da fotobiomodulação na reação local induzida pelo veneno da serpente Bothrops alternatus em seus estágios juvenil e adulto
dc.title.alternative.eng.fl_str_mv Effect of photobiomodulation on local reaction induced by the venon of Bothrops alternatus on its juvenile and adult stages
title Efeito da fotobiomodulação na reação local induzida pelo veneno da serpente Bothrops alternatus em seus estágios juvenil e adulto
spellingShingle Efeito da fotobiomodulação na reação local induzida pelo veneno da serpente Bothrops alternatus em seus estágios juvenil e adulto
David, Amanda Cabral
Bothrops alternatus
juvenil e adulto
fotobiomodulação
miotoxicidade
citotoxicidade
veneno botrópico
Bothrops alternatus
juvenile and adult
photobiomodulation
myotoxicity
cytotoxicity
bothropic venom
CIENCIAS DA SAUDE
title_short Efeito da fotobiomodulação na reação local induzida pelo veneno da serpente Bothrops alternatus em seus estágios juvenil e adulto
title_full Efeito da fotobiomodulação na reação local induzida pelo veneno da serpente Bothrops alternatus em seus estágios juvenil e adulto
title_fullStr Efeito da fotobiomodulação na reação local induzida pelo veneno da serpente Bothrops alternatus em seus estágios juvenil e adulto
title_full_unstemmed Efeito da fotobiomodulação na reação local induzida pelo veneno da serpente Bothrops alternatus em seus estágios juvenil e adulto
title_sort Efeito da fotobiomodulação na reação local induzida pelo veneno da serpente Bothrops alternatus em seus estágios juvenil e adulto
author David, Amanda Cabral
author_facet David, Amanda Cabral
author_role author
dc.contributor.advisor1.fl_str_mv Zamuner, Stella Regina
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/1935805744318404
dc.contributor.advisor-co1.fl_str_mv Zuliani, Juliana Pavan
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/9093880214338747
dc.contributor.referee1.fl_str_mv Zamuner, Stella Regina
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/1935805744318404
dc.contributor.referee2.fl_str_mv Dellê, Humberto
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/7435662740477057
dc.contributor.referee3.fl_str_mv Leiva, Laura C.
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9589613288491092
dc.contributor.author.fl_str_mv David, Amanda Cabral
contributor_str_mv Zamuner, Stella Regina
Zuliani, Juliana Pavan
Zamuner, Stella Regina
Dellê, Humberto
Leiva, Laura C.
dc.subject.por.fl_str_mv Bothrops alternatus
juvenil e adulto
fotobiomodulação
miotoxicidade
citotoxicidade
veneno botrópico
topic Bothrops alternatus
juvenil e adulto
fotobiomodulação
miotoxicidade
citotoxicidade
veneno botrópico
Bothrops alternatus
juvenile and adult
photobiomodulation
myotoxicity
cytotoxicity
bothropic venom
CIENCIAS DA SAUDE
dc.subject.eng.fl_str_mv Bothrops alternatus
juvenile and adult
photobiomodulation
myotoxicity
cytotoxicity
bothropic venom
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE
description Bothrops snakes are responsible for most snakebites in Latin America. Bothropic envenoming causes both systemic and severe local effects. Furthermore, it is described that venoms from young and adult snakes have differences in their composition, which can lead to differences in the evolution of the local picture. Bothropic antivenom is the treatment of first choice, however, its effectiveness is limited, being able to neutralize only the systemic effects. It has been shown in previous studies that photobiomodulation (PBM) is promising in reducing local effects induced by bothropic snakes. The aim of this study was to expand the knowledge of the actions of low-level laser (LLL) on the local effect, after the action of the snake venom Bothrops alternatus (VBa), in its juvenile (VBaJ) and adult (VBaA) stages. For this, in vivo experiments were carried out with Balb-C mice and, in vitro with C2C12 muscle cells. For the treatment, LLL, 660 nm, 100 mW and 40 seconds of irradiation were used. In vivo, the animals were injected with VBaJ or VBaA, or only with saline, and the groups that received PBM, the treatment was conducted 30 minutes after the injection of the venoms. After 6 hours, edema, inflammatory infiltrate, myotoxicity and histology of the gastrocnemius muscle were analyzed, and after 1, 3, 6 and 24 hours, hyperalgesia was analyzed. In vitro, C2C12 cells received either VBaJ or VBaA, or culture medium alone, and were immediately irradiated with LLL. After 3 hours of the action of the venoms, cell viability, cytotoxicity, differentiation, membrane potential, proliferation, and cellular apoptosis/necrosis were evaluated. It was observed that, in vivo, VBaJ and VBaA, at the applied doses, were able to induce edema, inflammatory infiltrate, hemorrhage, to increase hyperalgesia and to cause myotoxicity and myonecrosis, at different intensities. In vitro, the two venoms also caused cytotoxicity, decreased cell differentiation, increased cell proliferation, and also induced apoptosis. Treatment with PBM, in vivo, was effective in reducing myotoxicity, leukocyte migration and hyperalgesia caused by the two venoms tested. Furthermore, it reduced the edema caused by VBaJ. In vitro, PBM induced cell differentiation and proliferation and also reduced apoptotic and necrotic cells. It can be concluded that, although both venoms (juvenile and adult) are capable to induce local reactions, there is a difference in the magnitude of these effects, both in vivo and in vitro, and treatment with PBM was shown to be beneficial in reducing these local reactions induced by the venoms of this snake.
publishDate 2021
dc.date.issued.fl_str_mv 2021-12-16
dc.date.accessioned.fl_str_mv 2024-04-08T19:14:01Z
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dc.identifier.citation.fl_str_mv David, Amanda Cabral. Efeito da fotobiomodulação na reação local induzida pelo veneno da serpente Bothrops alternatus em seus estágios juvenil e adulto. 2021. 99 f. Dissertação( Programa de Mestrado em Medicina) - Universidade Nove de Julho, São Paulo.
dc.identifier.uri.fl_str_mv http://bibliotecatede.uninove.br/handle/tede/3307
identifier_str_mv David, Amanda Cabral. Efeito da fotobiomodulação na reação local induzida pelo veneno da serpente Bothrops alternatus em seus estágios juvenil e adulto. 2021. 99 f. Dissertação( Programa de Mestrado em Medicina) - Universidade Nove de Julho, São Paulo.
url http://bibliotecatede.uninove.br/handle/tede/3307
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dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Saúde
publisher.none.fl_str_mv Universidade Nove de Julho
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