Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/3003 |
Resumo: | Introduction: Spinal Cord Injury (SCI) is an impairment of the spinal cord that promotes the discontinuity of the spinal canal, leading to functional and sensory losses in the areas below the lesion, which are often irreversible. The effects of photobiomodulation (PBM) in the neuromuscular repair process show positive results, especially considering peripheral nerve injuries, however, in the recovery of spinal cord injuries, its effects are still poorly known and described in the literature, especially with regard to non-invasive systemic application, called vascular PBM (VPBM). Objective: Analyze the effects of VPBM in the immediate, acute and intermediate phases on functional (motor) recovery, tissue morphological aspects of neuromuscular repair, on the differential white blood cell count and on the protein expression of Brain Derived Neurotophic Factor (BDNF) after induction of SCI by compression. Methodology: 125 Wistar rats were used, divided into 5 groups: control group (n=5), SCI group (n=35), SCI+VPBM-I group (n=35), SCI+VPBM-2h group (n= 35) and SCI+VPBM-14d group (n=15). The VPBM was applied in the region of the caudal artery with the low-level laser (LLLT) AsGaAl (780 nm, 80 J/cm², 40 mW, for 80 seconds, totaling 3.2J energy in a single point) for 14 consecutive days. During the analysis periods (1, 3, 7, 14, 21, 28 and 35 days after SCI), the animals were evaluated for functionality using the Basso-Beattie- Bresnahan Index (BBB). At the end of each experimental period, blood samples were collected to evaluate the differential count of peripheral leukocytes and to evaluate the circulating concentration of BDNF by ELISA technique and for morphological and histological evaluation, muscle and nervous tissue samples were also extracted. Results: Treatment with VPBM-I and VPBM-2h led to the recovery of motor function, observed from the 7th day after injury (p<0.05), to an increase in the Cross-Sectional Area (CSA) in the second week of evaluation and to the diameter of the muscle fiber, observed from the 21st evaluation (p<0.05), it was also observed that early irradiation has more effect in reducing the cavity size with stabilization of the same being observed on the 28th day of treatment. evaluation (p<0.05), there was maintenance of baseline neutrophil values on the 1st day after SCI with a significant increase on the 3rd day after injury in the SCI+VPBM-2h group. In addition, an increase in BDNF expression was observed on the 7th day for the SCI+VPBM-I and SCI groups (p=0.0045 and p=0.0011 respectively), after 35 days the irradiated groups SCI+VPBM-I and SCI+VPBM- 2h showed BDNF levels that did not differ from the control. Conclusion: The results showed that VPBM was able to modulate the morphological and functional recovery after SCI, especially when applied early, showing positive effects on functional recovery evaluated by the BBB index and horizontal ladder, reestablishment of muscle tissue structure (diameter and AST) and nervous tissue (reduction of the cavity size in the spinal cord) and reduction of BDNF levels close to the values of the control group in the later phases evaluated, which can therefore be an important ally in the treatment of SCI. |
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Ferrari, Raquel Agnelli Mesquitahttp://lattes.cnpq.br/7048141683554943Ferrari, Raquel Agnelli Mesquitahttp://lattes.cnpq.br/7048141683554943Marcos, Rodrigo Labathttp://lattes.cnpq.br/2964112163504080Iemma, Mônica Rosas da CostaNavarro, Ricardo ScarparoBussadori, Sandra Kalilhttp://lattes.cnpq.br/4110914499694913Navarro, Ricardo Scarparohttp://lattes.cnpq.br/9120674464635588http://lattes.cnpq.br/7222998307651096Tobelem, Daysi da Cruz2022-07-26T20:06:04Z2022-06-27Tobelem, Daysi da Cruz. Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos. 2022. 89 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo.http://bibliotecatede.uninove.br/handle/tede/3003Introduction: Spinal Cord Injury (SCI) is an impairment of the spinal cord that promotes the discontinuity of the spinal canal, leading to functional and sensory losses in the areas below the lesion, which are often irreversible. The effects of photobiomodulation (PBM) in the neuromuscular repair process show positive results, especially considering peripheral nerve injuries, however, in the recovery of spinal cord injuries, its effects are still poorly known and described in the literature, especially with regard to non-invasive systemic application, called vascular PBM (VPBM). Objective: Analyze the effects of VPBM in the immediate, acute and intermediate phases on functional (motor) recovery, tissue morphological aspects of neuromuscular repair, on the differential white blood cell count and on the protein expression of Brain Derived Neurotophic Factor (BDNF) after induction of SCI by compression. Methodology: 125 Wistar rats were used, divided into 5 groups: control group (n=5), SCI group (n=35), SCI+VPBM-I group (n=35), SCI+VPBM-2h group (n= 35) and SCI+VPBM-14d group (n=15). The VPBM was applied in the region of the caudal artery with the low-level laser (LLLT) AsGaAl (780 nm, 80 J/cm², 40 mW, for 80 seconds, totaling 3.2J energy in a single point) for 14 consecutive days. During the analysis periods (1, 3, 7, 14, 21, 28 and 35 days after SCI), the animals were evaluated for functionality using the Basso-Beattie- Bresnahan Index (BBB). At the end of each experimental period, blood samples were collected to evaluate the differential count of peripheral leukocytes and to evaluate the circulating concentration of BDNF by ELISA technique and for morphological and histological evaluation, muscle and nervous tissue samples were also extracted. Results: Treatment with VPBM-I and VPBM-2h led to the recovery of motor function, observed from the 7th day after injury (p<0.05), to an increase in the Cross-Sectional Area (CSA) in the second week of evaluation and to the diameter of the muscle fiber, observed from the 21st evaluation (p<0.05), it was also observed that early irradiation has more effect in reducing the cavity size with stabilization of the same being observed on the 28th day of treatment. evaluation (p<0.05), there was maintenance of baseline neutrophil values on the 1st day after SCI with a significant increase on the 3rd day after injury in the SCI+VPBM-2h group. In addition, an increase in BDNF expression was observed on the 7th day for the SCI+VPBM-I and SCI groups (p=0.0045 and p=0.0011 respectively), after 35 days the irradiated groups SCI+VPBM-I and SCI+VPBM- 2h showed BDNF levels that did not differ from the control. Conclusion: The results showed that VPBM was able to modulate the morphological and functional recovery after SCI, especially when applied early, showing positive effects on functional recovery evaluated by the BBB index and horizontal ladder, reestablishment of muscle tissue structure (diameter and AST) and nervous tissue (reduction of the cavity size in the spinal cord) and reduction of BDNF levels close to the values of the control group in the later phases evaluated, which can therefore be an important ally in the treatment of SCI.Introdução: A Lesão Medular (LM) é um comprometimento da medula espinhal que promove a descontinuidade do canal medular, levando a perdas funcionais e sensitivas nas áreas abaixo da lesão, muitas vezes irreversíveis. Os efeitos da fotobiomodulação (FBM) no processo de reparo neuromuscular evidencia resultados positivos especialmente considerando lesões nervosas periféricas contudo na recuperação de lesões medulares seus efeitos ainda são pouco conhecidos e descritos na literatura, especialmente no que se refere a sua aplicação de forma sistêmica não invasiva denominada FBM vascular (FBMV). Objetivo: Analisar os efeitos da FBMV nas fases imediata, aguda e intermediária sobre a recuperação funcional (motora), aspectos morfológicos teciduais de reparo neuromuscular, sobre a contagem diferencial de glóbulos brancos e sobre a expressão proteica do Fator Neurotófico derivado do cérebro (BDNF) após indução de LM por compressão. Metodologia: Foram utilizados 125 ratos Wistar, divididos em 5 grupos: grupo controle (n=5), grupo LM (n=35), Grupo LM+FBMV-I (n=35), grupo LM+FBMV-2h (n=35) e grupo LM+FBMV14d (n=15). A FBMV foi aplicada na região da artéria caudal com o laser em baixa intensidade (LBI) AsGaAl (780 nm, 80 J/cm², 40 mW, por 80 segundos totalizando 3,2J energia em um único ponto) durante 14 dias consecutivos. Durante os períodos de análise (1, 3, 7, 14, 21, 28 e 35 dias após a LM), os animais foram avaliados quanto a funcionalidade pelo Índice de Basso-Beattie-Bresnahan (BBB). Ao final de cada período experimental foram coletadas amostras de sangue para avaliação da contagem diferencial dos leucócitos periféricos e para avaliação da concentração circulante do BDNF pela técnica de ELISA e para avaliação morfológica e histológica foram extraídas ainda amostras de tecido muscular, e nervoso. Resultados: O tratamento com FBMV-I e FBMV-2h levou a recuperação da função motora, observada a partir do 7º dia após lesão (p<0.05), ao aumento da Área de Secção Transversa (AST) na segunda semana de avaliação (p<0.05) e ao do diâmetro da fibra muscular, observada a partir da 21ª avaliação (p<0.05), observou-se ainda que a irradiação precoce tem mais efeito na redução do tamanho da cavidade, sendo observada estabilização da mesma no 28º dia de avaliação (p<0.05), houve ainda manutenção dos valores basais de neutrófilos no 1º dia após LM com aumento significativo dos mesmos no 3º dia após lesão no grupo LM+FBMV-2h. Além disso observou-se aumento de expressão do BDNF no 7º dia para os o grupo LM+FBMV- I e LM (p=0.0045 e p=0.0011 respectivamente), após 35 dias os grupos irradiados LM+FBMV-I e LM+FBM-2h apresentaram níveis de BDNF que não diferiram do controle. Conclusão: Os resultados evidenciaram que a FBMV foi capaz de modular a recuperação morfológica e funcional após LM especialmente quando aplicada precocemente evidenciando efeitos positivos sobre a recuperação funcional avaliada pelo índice BBB e escada horizontal, reestabelecimento da estrutura do tecido muscular (diâmetro e AST) e tecido nervoso (redução do tamanho da cavidade na medula) e redução dos níveis de BDNF próximo aos valores do grupo controle nas fases mais tardias avaliadas, podendo por tanto ser um importante aliado ao tratamento da LM.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2022-07-26T20:06:04Z No. of bitstreams: 1 Daysi da Cruz Tobelem.pdf: 2504028 bytes, checksum: 6454790dc948c798a9e2b31e5537cd50 (MD5)Made available in DSpace on 2022-07-26T20:06:04Z (GMT). No. of bitstreams: 1 Daysi da Cruz Tobelem.pdf: 2504028 bytes, checksum: 6454790dc948c798a9e2b31e5537cd50 (MD5) Previous issue date: 2022-06-27application/pdfporUniversidade Nove de JulhoPrograma de Pós-Graduação em Biofotônica Aplicada às Ciências da SaúdeUNINOVEBrasilSaúdelesão medularfotobiomodulaçãolaser de baixa potêncianeuroinflamaçãospinal cord injuryphotobiomodulationlow power laserneuroinflammationCIENCIAS DA SAUDEAvaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratosEvaluation of the effects of vascular photobiomodulation on neuromuscular repair and functional recovery after spinal compression injury in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALDaysi da Cruz Tobelem.pdfDaysi da Cruz Tobelem.pdfapplication/pdf2504028http://localhost:8080/tede/bitstream/tede/3003/2/Daysi+da+Cruz+Tobelem.pdf6454790dc948c798a9e2b31e5537cd50MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/3003/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/30032022-07-26 17:06:04.289oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2022-07-26T20:06:04Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos |
dc.title.alternative.eng.fl_str_mv |
Evaluation of the effects of vascular photobiomodulation on neuromuscular repair and functional recovery after spinal compression injury in rats |
title |
Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos |
spellingShingle |
Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos Tobelem, Daysi da Cruz lesão medular fotobiomodulação laser de baixa potência neuroinflamação spinal cord injury photobiomodulation low power laser neuroinflammation CIENCIAS DA SAUDE |
title_short |
Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos |
title_full |
Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos |
title_fullStr |
Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos |
title_full_unstemmed |
Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos |
title_sort |
Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos |
author |
Tobelem, Daysi da Cruz |
author_facet |
Tobelem, Daysi da Cruz |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Ferrari, Raquel Agnelli Mesquita |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7048141683554943 |
dc.contributor.referee1.fl_str_mv |
Ferrari, Raquel Agnelli Mesquita |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7048141683554943 |
dc.contributor.referee2.fl_str_mv |
Marcos, Rodrigo Labat |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/2964112163504080 |
dc.contributor.referee3.fl_str_mv |
Iemma, Mônica Rosas da Costa |
dc.contributor.referee3Lattes.fl_str_mv |
Navarro, Ricardo Scarparo |
dc.contributor.referee4.fl_str_mv |
Bussadori, Sandra Kalil |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/4110914499694913 |
dc.contributor.referee5.fl_str_mv |
Navarro, Ricardo Scarparo |
dc.contributor.referee5Lattes.fl_str_mv |
http://lattes.cnpq.br/9120674464635588 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7222998307651096 |
dc.contributor.author.fl_str_mv |
Tobelem, Daysi da Cruz |
contributor_str_mv |
Ferrari, Raquel Agnelli Mesquita Ferrari, Raquel Agnelli Mesquita Marcos, Rodrigo Labat Iemma, Mônica Rosas da Costa Bussadori, Sandra Kalil Navarro, Ricardo Scarparo |
dc.subject.por.fl_str_mv |
lesão medular fotobiomodulação laser de baixa potência neuroinflamação |
topic |
lesão medular fotobiomodulação laser de baixa potência neuroinflamação spinal cord injury photobiomodulation low power laser neuroinflammation CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
spinal cord injury photobiomodulation low power laser neuroinflammation |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Introduction: Spinal Cord Injury (SCI) is an impairment of the spinal cord that promotes the discontinuity of the spinal canal, leading to functional and sensory losses in the areas below the lesion, which are often irreversible. The effects of photobiomodulation (PBM) in the neuromuscular repair process show positive results, especially considering peripheral nerve injuries, however, in the recovery of spinal cord injuries, its effects are still poorly known and described in the literature, especially with regard to non-invasive systemic application, called vascular PBM (VPBM). Objective: Analyze the effects of VPBM in the immediate, acute and intermediate phases on functional (motor) recovery, tissue morphological aspects of neuromuscular repair, on the differential white blood cell count and on the protein expression of Brain Derived Neurotophic Factor (BDNF) after induction of SCI by compression. Methodology: 125 Wistar rats were used, divided into 5 groups: control group (n=5), SCI group (n=35), SCI+VPBM-I group (n=35), SCI+VPBM-2h group (n= 35) and SCI+VPBM-14d group (n=15). The VPBM was applied in the region of the caudal artery with the low-level laser (LLLT) AsGaAl (780 nm, 80 J/cm², 40 mW, for 80 seconds, totaling 3.2J energy in a single point) for 14 consecutive days. During the analysis periods (1, 3, 7, 14, 21, 28 and 35 days after SCI), the animals were evaluated for functionality using the Basso-Beattie- Bresnahan Index (BBB). At the end of each experimental period, blood samples were collected to evaluate the differential count of peripheral leukocytes and to evaluate the circulating concentration of BDNF by ELISA technique and for morphological and histological evaluation, muscle and nervous tissue samples were also extracted. Results: Treatment with VPBM-I and VPBM-2h led to the recovery of motor function, observed from the 7th day after injury (p<0.05), to an increase in the Cross-Sectional Area (CSA) in the second week of evaluation and to the diameter of the muscle fiber, observed from the 21st evaluation (p<0.05), it was also observed that early irradiation has more effect in reducing the cavity size with stabilization of the same being observed on the 28th day of treatment. evaluation (p<0.05), there was maintenance of baseline neutrophil values on the 1st day after SCI with a significant increase on the 3rd day after injury in the SCI+VPBM-2h group. In addition, an increase in BDNF expression was observed on the 7th day for the SCI+VPBM-I and SCI groups (p=0.0045 and p=0.0011 respectively), after 35 days the irradiated groups SCI+VPBM-I and SCI+VPBM- 2h showed BDNF levels that did not differ from the control. Conclusion: The results showed that VPBM was able to modulate the morphological and functional recovery after SCI, especially when applied early, showing positive effects on functional recovery evaluated by the BBB index and horizontal ladder, reestablishment of muscle tissue structure (diameter and AST) and nervous tissue (reduction of the cavity size in the spinal cord) and reduction of BDNF levels close to the values of the control group in the later phases evaluated, which can therefore be an important ally in the treatment of SCI. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-07-26T20:06:04Z |
dc.date.issued.fl_str_mv |
2022-06-27 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Tobelem, Daysi da Cruz. Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos. 2022. 89 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/3003 |
identifier_str_mv |
Tobelem, Daysi da Cruz. Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos. 2022. 89 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo. |
url |
http://bibliotecatede.uninove.br/handle/tede/3003 |
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por |
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por |
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8765449414823306929 |
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600 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Nove de Julho |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde |
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UNINOVE |
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Brasil |
dc.publisher.department.fl_str_mv |
Saúde |
publisher.none.fl_str_mv |
Universidade Nove de Julho |
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Universidade Nove de Julho (UNINOVE) |
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UNINOVE |
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UNINOVE |
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Biblioteca Digital de Teses e Dissertações da Uninove |
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Biblioteca Digital de Teses e Dissertações da Uninove |
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Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE) |
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bibliotecatede@uninove.br||bibliotecatede@uninove.br |
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1811016887972134912 |