Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos

Detalhes bibliográficos
Autor(a) principal: Tobelem, Daysi da Cruz
Data de Publicação: 2022
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da Uninove
Texto Completo: http://bibliotecatede.uninove.br/handle/tede/3003
Resumo: Introduction: Spinal Cord Injury (SCI) is an impairment of the spinal cord that promotes the discontinuity of the spinal canal, leading to functional and sensory losses in the areas below the lesion, which are often irreversible. The effects of photobiomodulation (PBM) in the neuromuscular repair process show positive results, especially considering peripheral nerve injuries, however, in the recovery of spinal cord injuries, its effects are still poorly known and described in the literature, especially with regard to non-invasive systemic application, called vascular PBM (VPBM). Objective: Analyze the effects of VPBM in the immediate, acute and intermediate phases on functional (motor) recovery, tissue morphological aspects of neuromuscular repair, on the differential white blood cell count and on the protein expression of Brain Derived Neurotophic Factor (BDNF) after induction of SCI by compression. Methodology: 125 Wistar rats were used, divided into 5 groups: control group (n=5), SCI group (n=35), SCI+VPBM-I group (n=35), SCI+VPBM-2h group (n= 35) and SCI+VPBM-14d group (n=15). The VPBM was applied in the region of the caudal artery with the low-level laser (LLLT) AsGaAl (780 nm, 80 J/cm², 40 mW, for 80 seconds, totaling 3.2J energy in a single point) for 14 consecutive days. During the analysis periods (1, 3, 7, 14, 21, 28 and 35 days after SCI), the animals were evaluated for functionality using the Basso-Beattie- Bresnahan Index (BBB). At the end of each experimental period, blood samples were collected to evaluate the differential count of peripheral leukocytes and to evaluate the circulating concentration of BDNF by ELISA technique and for morphological and histological evaluation, muscle and nervous tissue samples were also extracted. Results: Treatment with VPBM-I and VPBM-2h led to the recovery of motor function, observed from the 7th day after injury (p<0.05), to an increase in the Cross-Sectional Area (CSA) in the second week of evaluation and to the diameter of the muscle fiber, observed from the 21st evaluation (p<0.05), it was also observed that early irradiation has more effect in reducing the cavity size with stabilization of the same being observed on the 28th day of treatment. evaluation (p<0.05), there was maintenance of baseline neutrophil values on the 1st day after SCI with a significant increase on the 3rd day after injury in the SCI+VPBM-2h group. In addition, an increase in BDNF expression was observed on the 7th day for the SCI+VPBM-I and SCI groups (p=0.0045 and p=0.0011 respectively), after 35 days the irradiated groups SCI+VPBM-I and SCI+VPBM- 2h showed BDNF levels that did not differ from the control. Conclusion: The results showed that VPBM was able to modulate the morphological and functional recovery after SCI, especially when applied early, showing positive effects on functional recovery evaluated by the BBB index and horizontal ladder, reestablishment of muscle tissue structure (diameter and AST) and nervous tissue (reduction of the cavity size in the spinal cord) and reduction of BDNF levels close to the values of the control group in the later phases evaluated, which can therefore be an important ally in the treatment of SCI.
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spelling Ferrari, Raquel Agnelli Mesquitahttp://lattes.cnpq.br/7048141683554943Ferrari, Raquel Agnelli Mesquitahttp://lattes.cnpq.br/7048141683554943Marcos, Rodrigo Labathttp://lattes.cnpq.br/2964112163504080Iemma, Mônica Rosas da CostaNavarro, Ricardo ScarparoBussadori, Sandra Kalilhttp://lattes.cnpq.br/4110914499694913Navarro, Ricardo Scarparohttp://lattes.cnpq.br/9120674464635588http://lattes.cnpq.br/7222998307651096Tobelem, Daysi da Cruz2022-07-26T20:06:04Z2022-06-27Tobelem, Daysi da Cruz. Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos. 2022. 89 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo.http://bibliotecatede.uninove.br/handle/tede/3003Introduction: Spinal Cord Injury (SCI) is an impairment of the spinal cord that promotes the discontinuity of the spinal canal, leading to functional and sensory losses in the areas below the lesion, which are often irreversible. The effects of photobiomodulation (PBM) in the neuromuscular repair process show positive results, especially considering peripheral nerve injuries, however, in the recovery of spinal cord injuries, its effects are still poorly known and described in the literature, especially with regard to non-invasive systemic application, called vascular PBM (VPBM). Objective: Analyze the effects of VPBM in the immediate, acute and intermediate phases on functional (motor) recovery, tissue morphological aspects of neuromuscular repair, on the differential white blood cell count and on the protein expression of Brain Derived Neurotophic Factor (BDNF) after induction of SCI by compression. Methodology: 125 Wistar rats were used, divided into 5 groups: control group (n=5), SCI group (n=35), SCI+VPBM-I group (n=35), SCI+VPBM-2h group (n= 35) and SCI+VPBM-14d group (n=15). The VPBM was applied in the region of the caudal artery with the low-level laser (LLLT) AsGaAl (780 nm, 80 J/cm², 40 mW, for 80 seconds, totaling 3.2J energy in a single point) for 14 consecutive days. During the analysis periods (1, 3, 7, 14, 21, 28 and 35 days after SCI), the animals were evaluated for functionality using the Basso-Beattie- Bresnahan Index (BBB). At the end of each experimental period, blood samples were collected to evaluate the differential count of peripheral leukocytes and to evaluate the circulating concentration of BDNF by ELISA technique and for morphological and histological evaluation, muscle and nervous tissue samples were also extracted. Results: Treatment with VPBM-I and VPBM-2h led to the recovery of motor function, observed from the 7th day after injury (p<0.05), to an increase in the Cross-Sectional Area (CSA) in the second week of evaluation and to the diameter of the muscle fiber, observed from the 21st evaluation (p<0.05), it was also observed that early irradiation has more effect in reducing the cavity size with stabilization of the same being observed on the 28th day of treatment. evaluation (p<0.05), there was maintenance of baseline neutrophil values on the 1st day after SCI with a significant increase on the 3rd day after injury in the SCI+VPBM-2h group. In addition, an increase in BDNF expression was observed on the 7th day for the SCI+VPBM-I and SCI groups (p=0.0045 and p=0.0011 respectively), after 35 days the irradiated groups SCI+VPBM-I and SCI+VPBM- 2h showed BDNF levels that did not differ from the control. Conclusion: The results showed that VPBM was able to modulate the morphological and functional recovery after SCI, especially when applied early, showing positive effects on functional recovery evaluated by the BBB index and horizontal ladder, reestablishment of muscle tissue structure (diameter and AST) and nervous tissue (reduction of the cavity size in the spinal cord) and reduction of BDNF levels close to the values of the control group in the later phases evaluated, which can therefore be an important ally in the treatment of SCI.Introdução: A Lesão Medular (LM) é um comprometimento da medula espinhal que promove a descontinuidade do canal medular, levando a perdas funcionais e sensitivas nas áreas abaixo da lesão, muitas vezes irreversíveis. Os efeitos da fotobiomodulação (FBM) no processo de reparo neuromuscular evidencia resultados positivos especialmente considerando lesões nervosas periféricas contudo na recuperação de lesões medulares seus efeitos ainda são pouco conhecidos e descritos na literatura, especialmente no que se refere a sua aplicação de forma sistêmica não invasiva denominada FBM vascular (FBMV). Objetivo: Analisar os efeitos da FBMV nas fases imediata, aguda e intermediária sobre a recuperação funcional (motora), aspectos morfológicos teciduais de reparo neuromuscular, sobre a contagem diferencial de glóbulos brancos e sobre a expressão proteica do Fator Neurotófico derivado do cérebro (BDNF) após indução de LM por compressão. Metodologia: Foram utilizados 125 ratos Wistar, divididos em 5 grupos: grupo controle (n=5), grupo LM (n=35), Grupo LM+FBMV-I (n=35), grupo LM+FBMV-2h (n=35) e grupo LM+FBMV14d (n=15). A FBMV foi aplicada na região da artéria caudal com o laser em baixa intensidade (LBI) AsGaAl (780 nm, 80 J/cm², 40 mW, por 80 segundos totalizando 3,2J energia em um único ponto) durante 14 dias consecutivos. Durante os períodos de análise (1, 3, 7, 14, 21, 28 e 35 dias após a LM), os animais foram avaliados quanto a funcionalidade pelo Índice de Basso-Beattie-Bresnahan (BBB). Ao final de cada período experimental foram coletadas amostras de sangue para avaliação da contagem diferencial dos leucócitos periféricos e para avaliação da concentração circulante do BDNF pela técnica de ELISA e para avaliação morfológica e histológica foram extraídas ainda amostras de tecido muscular, e nervoso. Resultados: O tratamento com FBMV-I e FBMV-2h levou a recuperação da função motora, observada a partir do 7º dia após lesão (p<0.05), ao aumento da Área de Secção Transversa (AST) na segunda semana de avaliação (p<0.05) e ao do diâmetro da fibra muscular, observada a partir da 21ª avaliação (p<0.05), observou-se ainda que a irradiação precoce tem mais efeito na redução do tamanho da cavidade, sendo observada estabilização da mesma no 28º dia de avaliação (p<0.05), houve ainda manutenção dos valores basais de neutrófilos no 1º dia após LM com aumento significativo dos mesmos no 3º dia após lesão no grupo LM+FBMV-2h. Além disso observou-se aumento de expressão do BDNF no 7º dia para os o grupo LM+FBMV- I e LM (p=0.0045 e p=0.0011 respectivamente), após 35 dias os grupos irradiados LM+FBMV-I e LM+FBM-2h apresentaram níveis de BDNF que não diferiram do controle. Conclusão: Os resultados evidenciaram que a FBMV foi capaz de modular a recuperação morfológica e funcional após LM especialmente quando aplicada precocemente evidenciando efeitos positivos sobre a recuperação funcional avaliada pelo índice BBB e escada horizontal, reestabelecimento da estrutura do tecido muscular (diâmetro e AST) e tecido nervoso (redução do tamanho da cavidade na medula) e redução dos níveis de BDNF próximo aos valores do grupo controle nas fases mais tardias avaliadas, podendo por tanto ser um importante aliado ao tratamento da LM.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2022-07-26T20:06:04Z No. of bitstreams: 1 Daysi da Cruz Tobelem.pdf: 2504028 bytes, checksum: 6454790dc948c798a9e2b31e5537cd50 (MD5)Made available in DSpace on 2022-07-26T20:06:04Z (GMT). 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dc.title.por.fl_str_mv Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos
dc.title.alternative.eng.fl_str_mv Evaluation of the effects of vascular photobiomodulation on neuromuscular repair and functional recovery after spinal compression injury in rats
title Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos
spellingShingle Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos
Tobelem, Daysi da Cruz
lesão medular
fotobiomodulação
laser de baixa potência
neuroinflamação
spinal cord injury
photobiomodulation
low power laser
neuroinflammation
CIENCIAS DA SAUDE
title_short Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos
title_full Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos
title_fullStr Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos
title_full_unstemmed Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos
title_sort Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos
author Tobelem, Daysi da Cruz
author_facet Tobelem, Daysi da Cruz
author_role author
dc.contributor.advisor1.fl_str_mv Ferrari, Raquel Agnelli Mesquita
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/7048141683554943
dc.contributor.referee1.fl_str_mv Ferrari, Raquel Agnelli Mesquita
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/7048141683554943
dc.contributor.referee2.fl_str_mv Marcos, Rodrigo Labat
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/2964112163504080
dc.contributor.referee3.fl_str_mv Iemma, Mônica Rosas da Costa
dc.contributor.referee3Lattes.fl_str_mv Navarro, Ricardo Scarparo
dc.contributor.referee4.fl_str_mv Bussadori, Sandra Kalil
dc.contributor.referee4Lattes.fl_str_mv http://lattes.cnpq.br/4110914499694913
dc.contributor.referee5.fl_str_mv Navarro, Ricardo Scarparo
dc.contributor.referee5Lattes.fl_str_mv http://lattes.cnpq.br/9120674464635588
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7222998307651096
dc.contributor.author.fl_str_mv Tobelem, Daysi da Cruz
contributor_str_mv Ferrari, Raquel Agnelli Mesquita
Ferrari, Raquel Agnelli Mesquita
Marcos, Rodrigo Labat
Iemma, Mônica Rosas da Costa
Bussadori, Sandra Kalil
Navarro, Ricardo Scarparo
dc.subject.por.fl_str_mv lesão medular
fotobiomodulação
laser de baixa potência
neuroinflamação
topic lesão medular
fotobiomodulação
laser de baixa potência
neuroinflamação
spinal cord injury
photobiomodulation
low power laser
neuroinflammation
CIENCIAS DA SAUDE
dc.subject.eng.fl_str_mv spinal cord injury
photobiomodulation
low power laser
neuroinflammation
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE
description Introduction: Spinal Cord Injury (SCI) is an impairment of the spinal cord that promotes the discontinuity of the spinal canal, leading to functional and sensory losses in the areas below the lesion, which are often irreversible. The effects of photobiomodulation (PBM) in the neuromuscular repair process show positive results, especially considering peripheral nerve injuries, however, in the recovery of spinal cord injuries, its effects are still poorly known and described in the literature, especially with regard to non-invasive systemic application, called vascular PBM (VPBM). Objective: Analyze the effects of VPBM in the immediate, acute and intermediate phases on functional (motor) recovery, tissue morphological aspects of neuromuscular repair, on the differential white blood cell count and on the protein expression of Brain Derived Neurotophic Factor (BDNF) after induction of SCI by compression. Methodology: 125 Wistar rats were used, divided into 5 groups: control group (n=5), SCI group (n=35), SCI+VPBM-I group (n=35), SCI+VPBM-2h group (n= 35) and SCI+VPBM-14d group (n=15). The VPBM was applied in the region of the caudal artery with the low-level laser (LLLT) AsGaAl (780 nm, 80 J/cm², 40 mW, for 80 seconds, totaling 3.2J energy in a single point) for 14 consecutive days. During the analysis periods (1, 3, 7, 14, 21, 28 and 35 days after SCI), the animals were evaluated for functionality using the Basso-Beattie- Bresnahan Index (BBB). At the end of each experimental period, blood samples were collected to evaluate the differential count of peripheral leukocytes and to evaluate the circulating concentration of BDNF by ELISA technique and for morphological and histological evaluation, muscle and nervous tissue samples were also extracted. Results: Treatment with VPBM-I and VPBM-2h led to the recovery of motor function, observed from the 7th day after injury (p<0.05), to an increase in the Cross-Sectional Area (CSA) in the second week of evaluation and to the diameter of the muscle fiber, observed from the 21st evaluation (p<0.05), it was also observed that early irradiation has more effect in reducing the cavity size with stabilization of the same being observed on the 28th day of treatment. evaluation (p<0.05), there was maintenance of baseline neutrophil values on the 1st day after SCI with a significant increase on the 3rd day after injury in the SCI+VPBM-2h group. In addition, an increase in BDNF expression was observed on the 7th day for the SCI+VPBM-I and SCI groups (p=0.0045 and p=0.0011 respectively), after 35 days the irradiated groups SCI+VPBM-I and SCI+VPBM- 2h showed BDNF levels that did not differ from the control. Conclusion: The results showed that VPBM was able to modulate the morphological and functional recovery after SCI, especially when applied early, showing positive effects on functional recovery evaluated by the BBB index and horizontal ladder, reestablishment of muscle tissue structure (diameter and AST) and nervous tissue (reduction of the cavity size in the spinal cord) and reduction of BDNF levels close to the values of the control group in the later phases evaluated, which can therefore be an important ally in the treatment of SCI.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-07-26T20:06:04Z
dc.date.issued.fl_str_mv 2022-06-27
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv Tobelem, Daysi da Cruz. Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos. 2022. 89 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo.
dc.identifier.uri.fl_str_mv http://bibliotecatede.uninove.br/handle/tede/3003
identifier_str_mv Tobelem, Daysi da Cruz. Avaliação dos efeitos da fotobiomodulação vascular no reparo neuromuscular e recuperação funcional após a lesão medular por compressão em ratos. 2022. 89 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo.
url http://bibliotecatede.uninove.br/handle/tede/3003
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