Efeito do anabolizante decanoato de nadrolona na viabilidade, prolifera????o, ades??o e diferencia????o em c??lulas musculares C2C12.
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/tede/handle/tede/826 |
Resumo: | Studies indicate that the anabolic nandrolone decanoate modulates cell cycle regulation, but little is known about its effects on muscle cells. The aim of this study was to evaluate the effect of anabolic nandrolone decanoate on proliferation, adhesion and differentiation of C2C12 skeletal muscle cells. The cells were cultured in a regular, 10% fetal bovine serum (FBS) and simulation of nutritional deficiency (5% FBS) and incubated with the steroid / vehicle at concentrations of 5, 10, 25 and 50??M. The control group did not receive the treatment. Cell proliferation was assessed in 24, 48 and 72 (short term) and 3 and 5 days (long-term), and the adhesion in 20, 40 and 60 min, using the MTT assay. The myogenic markers expression was assessed after 2, 4 and 8 hours using real time PCR. The results show that nandrolone decanoate did not induce significant changes in cell proliferation in short-term adherence. In the long term after 5 days there was a statistically significant increase in proliferation of cells treated with the anabolic 5??M when cultured in a regular situation and 25??M in simulation of injury, compared with the other concentrations and control. Furthermore, after 8 hours incubation, C2C12 cells showed an increased expression of myogenin in comparison to other periods while MyoD did not change during periods and study groups, no differences between the treated and control groups. Conclusion: The anabolic steroid nandrolone induced an increase in cell proliferation after five days in a dose-dependent but did not interfere in the adhesion and expression of myogenic markers. |
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Ferrari, Raquel Agnelli Mesquitahttp://lattes.cnpq.br/7048141683554943Fernandes, Kristiane Santos PortaRenno, Ana Claudia Munizhttp://lattes.cnpq.br/4106611304688552Bussadori, Sandra KalilCPF:00000000000http://lattes.cnpq.br/4110914499694913CPF:16346660896Oliveira, Elisangela Nascimento de2015-04-22T17:20:26Z2011-07-042010-12-15OLIVEIRA, Elisangela Nascimento de. Effect of the anabolizante decanoato of nadrolona in the viability, proliferation, adhesion and differentiation in muscular cells C2C12.. 2010. 64 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2010.http://bibliotecatede.uninove.br/tede/handle/tede/826Studies indicate that the anabolic nandrolone decanoate modulates cell cycle regulation, but little is known about its effects on muscle cells. The aim of this study was to evaluate the effect of anabolic nandrolone decanoate on proliferation, adhesion and differentiation of C2C12 skeletal muscle cells. The cells were cultured in a regular, 10% fetal bovine serum (FBS) and simulation of nutritional deficiency (5% FBS) and incubated with the steroid / vehicle at concentrations of 5, 10, 25 and 50??M. The control group did not receive the treatment. Cell proliferation was assessed in 24, 48 and 72 (short term) and 3 and 5 days (long-term), and the adhesion in 20, 40 and 60 min, using the MTT assay. The myogenic markers expression was assessed after 2, 4 and 8 hours using real time PCR. The results show that nandrolone decanoate did not induce significant changes in cell proliferation in short-term adherence. In the long term after 5 days there was a statistically significant increase in proliferation of cells treated with the anabolic 5??M when cultured in a regular situation and 25??M in simulation of injury, compared with the other concentrations and control. Furthermore, after 8 hours incubation, C2C12 cells showed an increased expression of myogenin in comparison to other periods while MyoD did not change during periods and study groups, no differences between the treated and control groups. Conclusion: The anabolic steroid nandrolone induced an increase in cell proliferation after five days in a dose-dependent but did not interfere in the adhesion and expression of myogenic markers.Estudos apontam que o anabolizante decanoato de nandrolona modula a regula????o do ciclo celular, por??m pouco se conhece sobre seus efeitos nas c??lulas musculares. Assim, o objetivo foi avaliar o efeito do anabolizante decanoato de nandrolona na prolifera????o, ades??o e diferencia????o das c??lulas musculares C2C12. As c??lulas foram cultivadas em meio regular, 10% de soro fetal bovino (SFB) e em car??ncia nutricional (5% SFB) e incubadas com o anabolizante/ve??culo nas concentra????es de 5, 10, 25 e 50??M. O grupo controle n??o recebeu tratamento. A prolifera????o celular foi avaliada em 24, 48 e 72h (curto prazo) e em 3 e 5 dias (longo prazo), e a ades??o celular em 20, 40 e 60 min, utilizando o m??todo MTT. A express??o dos marcadores miog??nicos foi avaliada ap??s 2, 4 e 8h utilizando o PCR em tempo real. Os resultados mostraram que o decanoato de nandrolona n??o induziu altera????es significativas na prolifera????o celular em curto prazo e na ades??o. Em longo prazo ap??s 5 dias houve um aumento estatisticamente significativo na prolifera????o das c??lulas tratadas com anabolizante a 5??M quando cultivadas em meio regular e 25??M na situa????o de simula????o de les??o, com rela????o ??s demais concentra????es e ao controle. Al??m disso, ap??s 8 horas da incuba????o as c??lulas C2C12 apresentaram um aumento na express??o da miogenina em compara????o aos demais per??odos enquanto que a express??o de MyoD n??o sofreu altera????o nos per??odos e grupos avaliados, n??o havendo diferen??as entre os grupos tratados e controle. Conclus??o: o anabolizante decanoato de nandrolona induziu um aumento na prolifera????o celular ap??s 5 dias de forma dose-dependente, por??m n??o interferiu na ades??o celular e express??o de marcadores miog??nicos.Made available in DSpace on 2015-04-22T17:20:26Z (GMT). No. of bitstreams: 1 B_Elisangela Nascimento de Oliveira.pdf: 438699 bytes, checksum: cde14fa29f6a2e60153ff40d34fcf27b (MD5) Previous issue date: 2010-12-15application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBRSa??dedecanoato de nandrolonamioblastosprolifera????oades??odiferencia????onandrolone decanoatemyoblastsproliferationadhesiondiferentiationCIENCIAS DA SAUDEEfeito do anabolizante decanoato de nadrolona na viabilidade, prolifera????o, ades??o e diferencia????o em c??lulas musculares C2C12.Effect of the anabolizante decanoato of nadrolona in the viability, proliferation, adhesion and differentiation in muscular cells C2C12.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALB_Elisangela Nascimento de Oliveira.pdfapplication/pdf438699http://localhost:8080/tede/bitstream/tede/826/1/B_Elisangela+Nascimento+de+Oliveira.pdfcde14fa29f6a2e60153ff40d34fcf27bMD51tede/8262018-06-15 12:45:49.984oai:localhost:tede/826Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-15T15:45:49Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito do anabolizante decanoato de nadrolona na viabilidade, prolifera????o, ades??o e diferencia????o em c??lulas musculares C2C12. |
dc.title.alternative.eng.fl_str_mv |
Effect of the anabolizante decanoato of nadrolona in the viability, proliferation, adhesion and differentiation in muscular cells C2C12. |
title |
Efeito do anabolizante decanoato de nadrolona na viabilidade, prolifera????o, ades??o e diferencia????o em c??lulas musculares C2C12. |
spellingShingle |
Efeito do anabolizante decanoato de nadrolona na viabilidade, prolifera????o, ades??o e diferencia????o em c??lulas musculares C2C12. Oliveira, Elisangela Nascimento de decanoato de nandrolona mioblastos prolifera????o ades??o diferencia????o nandrolone decanoate myoblasts proliferation adhesion diferentiation CIENCIAS DA SAUDE |
title_short |
Efeito do anabolizante decanoato de nadrolona na viabilidade, prolifera????o, ades??o e diferencia????o em c??lulas musculares C2C12. |
title_full |
Efeito do anabolizante decanoato de nadrolona na viabilidade, prolifera????o, ades??o e diferencia????o em c??lulas musculares C2C12. |
title_fullStr |
Efeito do anabolizante decanoato de nadrolona na viabilidade, prolifera????o, ades??o e diferencia????o em c??lulas musculares C2C12. |
title_full_unstemmed |
Efeito do anabolizante decanoato de nadrolona na viabilidade, prolifera????o, ades??o e diferencia????o em c??lulas musculares C2C12. |
title_sort |
Efeito do anabolizante decanoato de nadrolona na viabilidade, prolifera????o, ades??o e diferencia????o em c??lulas musculares C2C12. |
author |
Oliveira, Elisangela Nascimento de |
author_facet |
Oliveira, Elisangela Nascimento de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Ferrari, Raquel Agnelli Mesquita |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7048141683554943 |
dc.contributor.advisor-co1.fl_str_mv |
Fernandes, Kristiane Santos Porta |
dc.contributor.referee1.fl_str_mv |
Renno, Ana Claudia Muniz |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/4106611304688552 |
dc.contributor.referee2.fl_str_mv |
Bussadori, Sandra Kalil |
dc.contributor.referee2ID.fl_str_mv |
CPF:00000000000 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/4110914499694913 |
dc.contributor.authorID.fl_str_mv |
CPF:16346660896 |
dc.contributor.author.fl_str_mv |
Oliveira, Elisangela Nascimento de |
contributor_str_mv |
Ferrari, Raquel Agnelli Mesquita Fernandes, Kristiane Santos Porta Renno, Ana Claudia Muniz Bussadori, Sandra Kalil |
dc.subject.por.fl_str_mv |
decanoato de nandrolona mioblastos prolifera????o ades??o diferencia????o |
topic |
decanoato de nandrolona mioblastos prolifera????o ades??o diferencia????o nandrolone decanoate myoblasts proliferation adhesion diferentiation CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
nandrolone decanoate myoblasts proliferation adhesion diferentiation |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Studies indicate that the anabolic nandrolone decanoate modulates cell cycle regulation, but little is known about its effects on muscle cells. The aim of this study was to evaluate the effect of anabolic nandrolone decanoate on proliferation, adhesion and differentiation of C2C12 skeletal muscle cells. The cells were cultured in a regular, 10% fetal bovine serum (FBS) and simulation of nutritional deficiency (5% FBS) and incubated with the steroid / vehicle at concentrations of 5, 10, 25 and 50??M. The control group did not receive the treatment. Cell proliferation was assessed in 24, 48 and 72 (short term) and 3 and 5 days (long-term), and the adhesion in 20, 40 and 60 min, using the MTT assay. The myogenic markers expression was assessed after 2, 4 and 8 hours using real time PCR. The results show that nandrolone decanoate did not induce significant changes in cell proliferation in short-term adherence. In the long term after 5 days there was a statistically significant increase in proliferation of cells treated with the anabolic 5??M when cultured in a regular situation and 25??M in simulation of injury, compared with the other concentrations and control. Furthermore, after 8 hours incubation, C2C12 cells showed an increased expression of myogenin in comparison to other periods while MyoD did not change during periods and study groups, no differences between the treated and control groups. Conclusion: The anabolic steroid nandrolone induced an increase in cell proliferation after five days in a dose-dependent but did not interfere in the adhesion and expression of myogenic markers. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-12-15 |
dc.date.available.fl_str_mv |
2011-07-04 |
dc.date.accessioned.fl_str_mv |
2015-04-22T17:20:26Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Elisangela Nascimento de. Effect of the anabolizante decanoato of nadrolona in the viability, proliferation, adhesion and differentiation in muscular cells C2C12.. 2010. 64 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2010. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/tede/handle/tede/826 |
identifier_str_mv |
OLIVEIRA, Elisangela Nascimento de. Effect of the anabolizante decanoato of nadrolona in the viability, proliferation, adhesion and differentiation in muscular cells C2C12.. 2010. 64 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2010. |
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http://bibliotecatede.uninove.br/tede/handle/tede/826 |
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