Efeito do laser em baixa intensidade sobre a prolifera????o, ades??o e express??o de Runx2 em osteoblastos cultivados na presen??a de LPS
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/tede/handle/tede/867 |
Resumo: | The tissue destruction associated with bone infection is related to the constituents of infectious microorganisms, their products and the activation of secretory cells of the tissue itself and the immune system. The lipopolysaccharide (LPS), wall component of gram-negative bacteria, was the first whose potent ability to induce bone resorption was demonstrated. On the other hand, the low level laser therapy (LLLT) has been used in bone tissue in order to accelerate the repair process. Thus, the objective of this study was to evaluate the effects of LLLT on cell parameters important in the process of bone repair front of infection. To this end, the osteoblast lineage OSTEO-1 (derived from rat calvaria), grown in the presence of LPS (E. coli) were irradiated with LLLT (Ga-Al-As, 780 nm, 10 mW, 12s, 0.12 J , 3 J/cm2). Cultures irradiated and not irradiated (control) were subjected to proliferation assays at 1, 3 and 5 days (MTT) and adhesion after 20, 40 and 60 minutes (MTT). In addition, the gene expression of the transcription factor run-related (Runx2) was assessed after 24 h using the polymerase chain reaction (PCR) in real time. The results showed no statistically significant differences in relation to cell proliferation and the expression of Runx2. As for cell adhesion LPS decreased the adhesion in the groups cultured in the presence of concentrations of 1 and 10 ??g/mL after 20 min. and the concentration of 10 ??g/mL after 40 and 60 min.. In all periods evaluated LLLT was not able to change this trend. |
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Fernandes, Kristianne Porta SantosFerrari, Raquel Agnelli Mesquitahttp://lattes.cnpq.br/7048141683554943Carvalho, Regiane Albertini dehttp://lattes.cnpq.br/2515657728339113Carvalho, Fl??vio Aimbire Soares dehttp://lattes.cnpq.br/8216583445045638CPF:30510215807http://lattes.cnpq.br/2280629394502730Teixeira, Victor Perez2015-04-22T17:20:36Z2012-06-212011-12-12TEIXEIRA, Victor Perez. Effect of the laser in low intensity on the proliferation, adhesion and expression of runx2 in osteoblastos cultivated in the LPS presence.. 2011. 62 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2011.http://bibliotecatede.uninove.br/tede/handle/tede/867The tissue destruction associated with bone infection is related to the constituents of infectious microorganisms, their products and the activation of secretory cells of the tissue itself and the immune system. The lipopolysaccharide (LPS), wall component of gram-negative bacteria, was the first whose potent ability to induce bone resorption was demonstrated. On the other hand, the low level laser therapy (LLLT) has been used in bone tissue in order to accelerate the repair process. Thus, the objective of this study was to evaluate the effects of LLLT on cell parameters important in the process of bone repair front of infection. To this end, the osteoblast lineage OSTEO-1 (derived from rat calvaria), grown in the presence of LPS (E. coli) were irradiated with LLLT (Ga-Al-As, 780 nm, 10 mW, 12s, 0.12 J , 3 J/cm2). Cultures irradiated and not irradiated (control) were subjected to proliferation assays at 1, 3 and 5 days (MTT) and adhesion after 20, 40 and 60 minutes (MTT). In addition, the gene expression of the transcription factor run-related (Runx2) was assessed after 24 h using the polymerase chain reaction (PCR) in real time. The results showed no statistically significant differences in relation to cell proliferation and the expression of Runx2. As for cell adhesion LPS decreased the adhesion in the groups cultured in the presence of concentrations of 1 and 10 ??g/mL after 20 min. and the concentration of 10 ??g/mL after 40 and 60 min.. In all periods evaluated LLLT was not able to change this trend.A destrui????o tecidual associada aos quadros de infec????o ??ssea est?? relacionada aos constituintes dos microorganismos infectantes, aos seus produtos secret??rios e ?? ativa????o de c??lulas do pr??prio tecido e do sistema imunol??gico. O lipopolissacar??deo (LPS), componente da parede de bact??rias gram-negativas, foi o primeiro componente bacteriano cuja potente capacidade de induzir reabsor????o ??ssea foi demonstrada. Por outro lado, o laser em baixa intensidade (LBI) tem sido utilizado em tecidos ??sseos com o intuito de acelerar o processo de reparo. Deste modo, o objetivo deste estudo foi avaliar os efeitos do LBI sobre a prolifera????o, a ades??o e a diferencia????o de osteoblastos cultivados em situa????o de simula????o de infec????o. Para tanto, osteoblastos da linhagem OSTEO-1 (derivados de calv??ria de ratos), cultivados na presen??a LPS (E.coli), foram irradiados com LBI (Ga-Al-As, 780 nm, 10 mW, 12s, 0,12 J, 3 J/cm2). Culturas irradiadas e n??o irradiadas (controle) foram submetidas a ensaios de prolifera????o em per??odos de 1, 3 e 5 dias (MTT) e ades??o ap??s 20, 40 e 60 minutos (MTT). Al??m disso, a express??o g??nica do fator de transcri????o run-related (Runx2) foi avaliada ap??s 24 h da irradia????o utilizando a rea????o em cadeia da polimerase (PCR) em tempo real. Os resultados n??o mostraram diferen??as estatisticamente significantes em rela????o ?? prolifera????o celular e a express??o de Runx2. Quanto ?? ades??o celular o LPS diminuiu a ades??o nos grupos cultivados na presen??a das concentra????es de 1 e 10 ??g/mL ap??s 20 min. e na concentra????o de 10 ??g/mL ap??s 40 e 60 min. Em todos os per??odos avaliados o LBI n??o se mostrou capaz de alterar esta tend??ncia.Made available in DSpace on 2015-04-22T17:20:36Z (GMT). No. of bitstreams: 1 B_Victor Perez Teixeira.pdf: 2237562 bytes, checksum: 0b9fe6f9d563939d9f9b54683c395d8f (MD5) Previous issue date: 2011-12-12application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBRSa??deTerapia a Laser de Baixa IntensidadeOsteoblastos, Bact??rias Gram-negativasLaser Therapy, Low-LevelOsteoblastsGram-Negative BacteriaCIENCIAS DA SAUDEEfeito do laser em baixa intensidade sobre a prolifera????o, ades??o e express??o de Runx2 em osteoblastos cultivados na presen??a de LPSEffect of the laser in low intensity on the proliferation, adhesion and expression of Runx2 in osteoblastos cultivated in the LPS presenceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALB_Victor Perez Teixeira.pdfapplication/pdf2237562http://localhost:8080/tede/bitstream/tede/867/1/B_Victor+Perez+Teixeira.pdf0b9fe6f9d563939d9f9b54683c395d8fMD51tede/8672018-06-15 12:43:34.938oai:localhost:tede/867Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-15T15:43:34Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito do laser em baixa intensidade sobre a prolifera????o, ades??o e express??o de Runx2 em osteoblastos cultivados na presen??a de LPS |
dc.title.alternative.eng.fl_str_mv |
Effect of the laser in low intensity on the proliferation, adhesion and expression of Runx2 in osteoblastos cultivated in the LPS presence |
title |
Efeito do laser em baixa intensidade sobre a prolifera????o, ades??o e express??o de Runx2 em osteoblastos cultivados na presen??a de LPS |
spellingShingle |
Efeito do laser em baixa intensidade sobre a prolifera????o, ades??o e express??o de Runx2 em osteoblastos cultivados na presen??a de LPS Teixeira, Victor Perez Terapia a Laser de Baixa Intensidade Osteoblastos, Bact??rias Gram-negativas Laser Therapy, Low-Level Osteoblasts Gram-Negative Bacteria CIENCIAS DA SAUDE |
title_short |
Efeito do laser em baixa intensidade sobre a prolifera????o, ades??o e express??o de Runx2 em osteoblastos cultivados na presen??a de LPS |
title_full |
Efeito do laser em baixa intensidade sobre a prolifera????o, ades??o e express??o de Runx2 em osteoblastos cultivados na presen??a de LPS |
title_fullStr |
Efeito do laser em baixa intensidade sobre a prolifera????o, ades??o e express??o de Runx2 em osteoblastos cultivados na presen??a de LPS |
title_full_unstemmed |
Efeito do laser em baixa intensidade sobre a prolifera????o, ades??o e express??o de Runx2 em osteoblastos cultivados na presen??a de LPS |
title_sort |
Efeito do laser em baixa intensidade sobre a prolifera????o, ades??o e express??o de Runx2 em osteoblastos cultivados na presen??a de LPS |
author |
Teixeira, Victor Perez |
author_facet |
Teixeira, Victor Perez |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Fernandes, Kristianne Porta Santos |
dc.contributor.advisor-co1.fl_str_mv |
Ferrari, Raquel Agnelli Mesquita |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/7048141683554943 |
dc.contributor.referee1.fl_str_mv |
Carvalho, Regiane Albertini de |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/2515657728339113 |
dc.contributor.referee2.fl_str_mv |
Carvalho, Fl??vio Aimbire Soares de |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/8216583445045638 |
dc.contributor.authorID.fl_str_mv |
CPF:30510215807 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2280629394502730 |
dc.contributor.author.fl_str_mv |
Teixeira, Victor Perez |
contributor_str_mv |
Fernandes, Kristianne Porta Santos Ferrari, Raquel Agnelli Mesquita Carvalho, Regiane Albertini de Carvalho, Fl??vio Aimbire Soares de |
dc.subject.por.fl_str_mv |
Terapia a Laser de Baixa Intensidade Osteoblastos, Bact??rias Gram-negativas |
topic |
Terapia a Laser de Baixa Intensidade Osteoblastos, Bact??rias Gram-negativas Laser Therapy, Low-Level Osteoblasts Gram-Negative Bacteria CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
Laser Therapy, Low-Level Osteoblasts Gram-Negative Bacteria |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
The tissue destruction associated with bone infection is related to the constituents of infectious microorganisms, their products and the activation of secretory cells of the tissue itself and the immune system. The lipopolysaccharide (LPS), wall component of gram-negative bacteria, was the first whose potent ability to induce bone resorption was demonstrated. On the other hand, the low level laser therapy (LLLT) has been used in bone tissue in order to accelerate the repair process. Thus, the objective of this study was to evaluate the effects of LLLT on cell parameters important in the process of bone repair front of infection. To this end, the osteoblast lineage OSTEO-1 (derived from rat calvaria), grown in the presence of LPS (E. coli) were irradiated with LLLT (Ga-Al-As, 780 nm, 10 mW, 12s, 0.12 J , 3 J/cm2). Cultures irradiated and not irradiated (control) were subjected to proliferation assays at 1, 3 and 5 days (MTT) and adhesion after 20, 40 and 60 minutes (MTT). In addition, the gene expression of the transcription factor run-related (Runx2) was assessed after 24 h using the polymerase chain reaction (PCR) in real time. The results showed no statistically significant differences in relation to cell proliferation and the expression of Runx2. As for cell adhesion LPS decreased the adhesion in the groups cultured in the presence of concentrations of 1 and 10 ??g/mL after 20 min. and the concentration of 10 ??g/mL after 40 and 60 min.. In all periods evaluated LLLT was not able to change this trend. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-12-12 |
dc.date.available.fl_str_mv |
2012-06-21 |
dc.date.accessioned.fl_str_mv |
2015-04-22T17:20:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
TEIXEIRA, Victor Perez. Effect of the laser in low intensity on the proliferation, adhesion and expression of runx2 in osteoblastos cultivated in the LPS presence.. 2011. 62 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2011. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/tede/handle/tede/867 |
identifier_str_mv |
TEIXEIRA, Victor Perez. Effect of the laser in low intensity on the proliferation, adhesion and expression of runx2 in osteoblastos cultivated in the LPS presence.. 2011. 62 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2011. |
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http://bibliotecatede.uninove.br/tede/handle/tede/867 |
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por |
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600 |
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openAccess |
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Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o |
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UNINOVE |
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