Efeito da toxina ur??mica indoxil sulfato em cultura de mioblastos c2c12 tratados ou n??o com laser de baixa pot??ncia
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/1298 |
Resumo: | Chronic kidney disease (CKD) is characterized by progressive and irreversible loss of renal function and often progresses with a muscular weakness, whose set of signs and symptoms is generally referred to as uremic myopathy. Possible risk factors for the uremic myopathy are the uremic toxins. Among uremic toxins, indoxyl sulfate (IS) is a derivative of tryptophan metabolism by intestinal bacteria. Because skeletal muscle tissue undergo constant remodeling due differentiation of myoblasts in myotubes, it is possible that uremic toxins have a deleterious effect to influence this process, exacerbating the uremic myopathy. Low level laser therapy (LLLT) is regarded as a growth promoter feature widely used in the treatment of chronic diseases and has shown positive effects on the modulation of skeletal muscle repair process and also in the process of inflammation. However, in the context of CKD, the LLLT has not yet been explored. The aim of this study was to evaluate the effects of the IS on cell viability and on oxidative stress on cellular differentiation in cultured C2C12 myoblasts. In addition, to verify the action of the LLLT as a protective alternative to the cells. The C2C12 myoblasts were cultured in DMEM culture medium containing 10% fetal bovine serum and were induced to differentiation process by adding 2% horse serum. Three different IS concentrations were used to mimic the plasma concentrations of normal individual, CKD patients with moderate uremia and CKD patients with advanced uremia (0.6 mg/l and 53 mg/l and 236 mg/l, respectively), at different times of incubation (24 h, 48 h and 72 h). Subsequently, the cells were subjected to treatment with LLLT GaAlAs 780 nm (output power 10 mW, 20 seconds application time and energy density of 0.5 J / cm2). In terms of analysis, we used MTT method to assess the viability of the cells, flow cytometry to assess the viability/cell death and oxidative stress, nitrite dosing to evaluate nitric oxide production and real-time PCR to analyze IL-6, myogenin and MyoD expression (inflammation and cell differentiation markers). The results demonstrate that the IS at the maximum concentration was toxic to C2C12 cells, because it significantly decreased cell viability by MTT and by flow cytometry and by increasing the percentage of necrosis. This effect was present throughout the three incubation periods. With respect to oxidative stress, was not any conclusion, probably by the time the samples, but do not rule out the possibility of IS induce this type of stress. Although the IS has induced death to C2C12 cells, the remaining had no change in cell differentiation markers. Treatment with BPL the IS sensitized cells, reducing cell viability. We conclude that the IS acts directly on C2C12 myoblasts with toxic effect and may be the one factor responsible for uremic myopathy. Treatment with LLLT was not effective in protecting the cells. |
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Dell??, HumbertoFernandes, Kristiane Porta SantosFerrari, Raquel Agrelli MesquitaDell??, Humbertohttp://lattes.cnpq.br/7435662740477057Reis, Luciene Machado doshttp://lattes.cnpq.br/6070147721873955Dalboni, Maria Aparecidahttp://lattes.cnpq.br/9818040147487320http://lattes.cnpq.br/5818336982053144Rodrigues, Gabriela Gomes Cardoso2016-05-17T20:42:43Z2015-02-04Rodrigues, Gabriela Gomes Cardoso. Efeito da toxina ur??mica indoxil sulfato em cultura de mioblastos c2c12 tratados ou n??o com laser de baixa pot??ncia. 2015. 70 f. Disserta????o( Programa de Mestrado em Medicina) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1298Chronic kidney disease (CKD) is characterized by progressive and irreversible loss of renal function and often progresses with a muscular weakness, whose set of signs and symptoms is generally referred to as uremic myopathy. Possible risk factors for the uremic myopathy are the uremic toxins. Among uremic toxins, indoxyl sulfate (IS) is a derivative of tryptophan metabolism by intestinal bacteria. Because skeletal muscle tissue undergo constant remodeling due differentiation of myoblasts in myotubes, it is possible that uremic toxins have a deleterious effect to influence this process, exacerbating the uremic myopathy. Low level laser therapy (LLLT) is regarded as a growth promoter feature widely used in the treatment of chronic diseases and has shown positive effects on the modulation of skeletal muscle repair process and also in the process of inflammation. However, in the context of CKD, the LLLT has not yet been explored. The aim of this study was to evaluate the effects of the IS on cell viability and on oxidative stress on cellular differentiation in cultured C2C12 myoblasts. In addition, to verify the action of the LLLT as a protective alternative to the cells. The C2C12 myoblasts were cultured in DMEM culture medium containing 10% fetal bovine serum and were induced to differentiation process by adding 2% horse serum. Three different IS concentrations were used to mimic the plasma concentrations of normal individual, CKD patients with moderate uremia and CKD patients with advanced uremia (0.6 mg/l and 53 mg/l and 236 mg/l, respectively), at different times of incubation (24 h, 48 h and 72 h). Subsequently, the cells were subjected to treatment with LLLT GaAlAs 780 nm (output power 10 mW, 20 seconds application time and energy density of 0.5 J / cm2). In terms of analysis, we used MTT method to assess the viability of the cells, flow cytometry to assess the viability/cell death and oxidative stress, nitrite dosing to evaluate nitric oxide production and real-time PCR to analyze IL-6, myogenin and MyoD expression (inflammation and cell differentiation markers). The results demonstrate that the IS at the maximum concentration was toxic to C2C12 cells, because it significantly decreased cell viability by MTT and by flow cytometry and by increasing the percentage of necrosis. This effect was present throughout the three incubation periods. With respect to oxidative stress, was not any conclusion, probably by the time the samples, but do not rule out the possibility of IS induce this type of stress. Although the IS has induced death to C2C12 cells, the remaining had no change in cell differentiation markers. Treatment with BPL the IS sensitized cells, reducing cell viability. We conclude that the IS acts directly on C2C12 myoblasts with toxic effect and may be the one factor responsible for uremic myopathy. Treatment with LLLT was not effective in protecting the cells.A doen??a renal cr??nica (DRC) ?? caracterizada pela perda progressiva e irrevers??vel da fun????o renal e que frequentemente cursa com um quadro de fraqueza muscular, cujo conjunto de sinais e sintomas ?? globalmente designado como miopatia ur??mica. Poss??veis fatores predisponentes para a miopatia ur??mica s??o as toxinas ur??micas. Dentre as toxinas ur??micas, o indoxil sulfato (IS) ?? uma derivada do metabolismo do triptofano presente em bact??rias intestinais. Devido ao fato do tecido muscular esquel??tico sofrer constante remodela????o gra??as ?? diferencia????o de mioblastos em miotubos, ?? poss??vel que toxinas ur??micas tenham um efeito delet??rio por influenciar este processo, agravando a miopatia ur??mica. A terapia a laser de baixa pot??ncia (LBP) ?? considerada como um recurso bioestimulante amplamente utilizado no tratamento de doen??as cr??nicas e tem demonstrado efeitos positivos sobre a modula????o do processo de reparo muscular esquel??tico e tamb??m no processo da inflama????o. Entretanto, no contexto de DRC, o LBP n??o foi ainda explorado. O objetivo do presente estudo foi avaliar dos efeitos do IS sobre a viabilidade celular, sobre o estresse oxidativo e sobre a diferencia????o celular em cultura de mioblastos C2C12. Al??m disso, verificar a a????o do LBP como forma de prote????o ??s c??lulas. Os mioblastos C2C12 foram cultivados em meio de cultura de DMEM, contendo 10% de soro fetal bovino e foram induzidos ao processo de diferencia????o por meio da adi????o de 2% soro de cavalo. Tr??s diferentes concentra????es de IS foram usadas para mimetizar as concentra????es plasm??ticas de indiv??duo normal, paciente DRC com uremia moderada e paciente DRC com uremia avan??ada (0,6 mg/l; 53 mg/l e 236 mg/l, respectivamente), em diferentes per??odos de incuba????o (24 h, 48 h e 72 h). Posteriormente, as c??lulas foram submetidas ao tratamento com laser de baixa pot??ncia AsGaAl 780 nm (pot??ncia de sa??da de 10 mW, tempo de aplica????o de 20 segundos e densidade de energia de 0,5 J/cm2). Como an??lise, foi utilizado o m??todo MTT para acessar a viabilidade das c??lulas, citometria de fluxo para avaliar a viabilidade/mortalidade das c??lulas, bem como o estresse oxidativo, dosagem de nitrito para avaliar a produ????o de ??xido n??trico e PCR em tempo real para analisar a express??o de IL-6, miogenina e MyoD (marcadores de inflama????o e diferencia????o celular). Os resultados demonstram que o IS na concentra????o m??xima foi t??xico para as c??lulas C2C12, pois diminuiu significativamente a viabilidade das c??lulas, tanto por MTT como por citometria de fluxo, aumentando a porcentagem de necrose. Este efeito foi presente nos tr??s per??odos de incuba????o. Com rela????o ao estresse oxidativo, n??o foi poss??vel nenhuma conclus??o, provavelmente pelo tempo das amostras , por??m n??o descartamos a possibilidade do IS induzir este tipo de estresse. Embora o IS tenha induzido morte ??s c??lulas C2C12, as remanescentes n??o tiveram altera????o dos marcadores de diferencia????o celular. O tratamento com LBP sensibilizou as c??lulas ao IS, diminuindo a viabilidade das c??lulas. Conclu??mos que o IS age diretamente sobre mioblastos C2C12 com efeito t??xico, podendo ser um dos respons??veis pela miopatia ur??mica. O tratamento com LBP n??o foi eficiente na indu????o de prote????o ??s c??lulas.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2016-05-17T20:42:43Z No. of bitstreams: 1 Gabriela Gomes Cardoso Rodrigues.pdf: 623885 bytes, checksum: 3da3d1230fd77beffc9c12f00be889bb (MD5)Made available in DSpace on 2016-05-17T20:42:43Z (GMT). No. of bitstreams: 1 Gabriela Gomes Cardoso Rodrigues.pdf: 623885 bytes, checksum: 3da3d1230fd77beffc9c12f00be889bb (MD5) Previous issue date: 2015-02-04application/pdfporUniversidade Nove de JulhoPrograma de Mestrado em MedicinaUNINOVEBrasilSa??demiopatia ur??micadoen??a renal cr??nicatoxinas ur??micasindoxil sulfatouremic myopathychronic kidney diseaseuremic toxinsindoxyl sulfateCIENCIAS DA SAUDEEfeito da toxina ur??mica indoxil sulfato em cultura de mioblastos c2c12 tratados ou n??o com laser de baixa pot??nciaEffect of toxin uremic indoxyl sulfate in myoblasty c2c12 culture or treated with no laser power lowinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALGabriela Gomes Cardoso Rodrigues.pdfGabriela Gomes Cardoso Rodrigues.pdfapplication/pdf668720http://localhost:8080/tede/bitstream/tede/1298/2/Gabriela+Gomes+Cardoso+Rodrigues.pdfa7e72ee2497a5cb0236d3785c1c8cdfbMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://localhost:8080/tede/bitstream/tede/1298/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/12982016-06-28 15:55:09.113oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2016-06-28T18:55:09Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito da toxina ur??mica indoxil sulfato em cultura de mioblastos c2c12 tratados ou n??o com laser de baixa pot??ncia |
dc.title.alternative.eng.fl_str_mv |
Effect of toxin uremic indoxyl sulfate in myoblasty c2c12 culture or treated with no laser power low |
title |
Efeito da toxina ur??mica indoxil sulfato em cultura de mioblastos c2c12 tratados ou n??o com laser de baixa pot??ncia |
spellingShingle |
Efeito da toxina ur??mica indoxil sulfato em cultura de mioblastos c2c12 tratados ou n??o com laser de baixa pot??ncia Rodrigues, Gabriela Gomes Cardoso miopatia ur??mica doen??a renal cr??nica toxinas ur??micas indoxil sulfato uremic myopathy chronic kidney disease uremic toxins indoxyl sulfate CIENCIAS DA SAUDE |
title_short |
Efeito da toxina ur??mica indoxil sulfato em cultura de mioblastos c2c12 tratados ou n??o com laser de baixa pot??ncia |
title_full |
Efeito da toxina ur??mica indoxil sulfato em cultura de mioblastos c2c12 tratados ou n??o com laser de baixa pot??ncia |
title_fullStr |
Efeito da toxina ur??mica indoxil sulfato em cultura de mioblastos c2c12 tratados ou n??o com laser de baixa pot??ncia |
title_full_unstemmed |
Efeito da toxina ur??mica indoxil sulfato em cultura de mioblastos c2c12 tratados ou n??o com laser de baixa pot??ncia |
title_sort |
Efeito da toxina ur??mica indoxil sulfato em cultura de mioblastos c2c12 tratados ou n??o com laser de baixa pot??ncia |
author |
Rodrigues, Gabriela Gomes Cardoso |
author_facet |
Rodrigues, Gabriela Gomes Cardoso |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Dell??, Humberto |
dc.contributor.advisor-co1.fl_str_mv |
Fernandes, Kristiane Porta Santos |
dc.contributor.advisor-co2.fl_str_mv |
Ferrari, Raquel Agrelli Mesquita |
dc.contributor.referee1.fl_str_mv |
Dell??, Humberto |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7435662740477057 |
dc.contributor.referee2.fl_str_mv |
Reis, Luciene Machado dos |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/6070147721873955 |
dc.contributor.referee3.fl_str_mv |
Dalboni, Maria Aparecida |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/9818040147487320 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5818336982053144 |
dc.contributor.author.fl_str_mv |
Rodrigues, Gabriela Gomes Cardoso |
contributor_str_mv |
Dell??, Humberto Fernandes, Kristiane Porta Santos Ferrari, Raquel Agrelli Mesquita Dell??, Humberto Reis, Luciene Machado dos Dalboni, Maria Aparecida |
dc.subject.por.fl_str_mv |
miopatia ur??mica doen??a renal cr??nica toxinas ur??micas indoxil sulfato |
topic |
miopatia ur??mica doen??a renal cr??nica toxinas ur??micas indoxil sulfato uremic myopathy chronic kidney disease uremic toxins indoxyl sulfate CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
uremic myopathy chronic kidney disease uremic toxins indoxyl sulfate |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Chronic kidney disease (CKD) is characterized by progressive and irreversible loss of renal function and often progresses with a muscular weakness, whose set of signs and symptoms is generally referred to as uremic myopathy. Possible risk factors for the uremic myopathy are the uremic toxins. Among uremic toxins, indoxyl sulfate (IS) is a derivative of tryptophan metabolism by intestinal bacteria. Because skeletal muscle tissue undergo constant remodeling due differentiation of myoblasts in myotubes, it is possible that uremic toxins have a deleterious effect to influence this process, exacerbating the uremic myopathy. Low level laser therapy (LLLT) is regarded as a growth promoter feature widely used in the treatment of chronic diseases and has shown positive effects on the modulation of skeletal muscle repair process and also in the process of inflammation. However, in the context of CKD, the LLLT has not yet been explored. The aim of this study was to evaluate the effects of the IS on cell viability and on oxidative stress on cellular differentiation in cultured C2C12 myoblasts. In addition, to verify the action of the LLLT as a protective alternative to the cells. The C2C12 myoblasts were cultured in DMEM culture medium containing 10% fetal bovine serum and were induced to differentiation process by adding 2% horse serum. Three different IS concentrations were used to mimic the plasma concentrations of normal individual, CKD patients with moderate uremia and CKD patients with advanced uremia (0.6 mg/l and 53 mg/l and 236 mg/l, respectively), at different times of incubation (24 h, 48 h and 72 h). Subsequently, the cells were subjected to treatment with LLLT GaAlAs 780 nm (output power 10 mW, 20 seconds application time and energy density of 0.5 J / cm2). In terms of analysis, we used MTT method to assess the viability of the cells, flow cytometry to assess the viability/cell death and oxidative stress, nitrite dosing to evaluate nitric oxide production and real-time PCR to analyze IL-6, myogenin and MyoD expression (inflammation and cell differentiation markers). The results demonstrate that the IS at the maximum concentration was toxic to C2C12 cells, because it significantly decreased cell viability by MTT and by flow cytometry and by increasing the percentage of necrosis. This effect was present throughout the three incubation periods. With respect to oxidative stress, was not any conclusion, probably by the time the samples, but do not rule out the possibility of IS induce this type of stress. Although the IS has induced death to C2C12 cells, the remaining had no change in cell differentiation markers. Treatment with BPL the IS sensitized cells, reducing cell viability. We conclude that the IS acts directly on C2C12 myoblasts with toxic effect and may be the one factor responsible for uremic myopathy. Treatment with LLLT was not effective in protecting the cells. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-02-04 |
dc.date.accessioned.fl_str_mv |
2016-05-17T20:42:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Rodrigues, Gabriela Gomes Cardoso. Efeito da toxina ur??mica indoxil sulfato em cultura de mioblastos c2c12 tratados ou n??o com laser de baixa pot??ncia. 2015. 70 f. Disserta????o( Programa de Mestrado em Medicina) - Universidade Nove de Julho, S??o Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/1298 |
identifier_str_mv |
Rodrigues, Gabriela Gomes Cardoso. Efeito da toxina ur??mica indoxil sulfato em cultura de mioblastos c2c12 tratados ou n??o com laser de baixa pot??ncia. 2015. 70 f. Disserta????o( Programa de Mestrado em Medicina) - Universidade Nove de Julho, S??o Paulo. |
url |
http://bibliotecatede.uninove.br/handle/tede/1298 |
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UNINOVE |
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bitstream.url.fl_str_mv |
http://localhost:8080/tede/bitstream/tede/1298/2/Gabriela+Gomes+Cardoso+Rodrigues.pdf http://localhost:8080/tede/bitstream/tede/1298/1/license.txt |
bitstream.checksum.fl_str_mv |
a7e72ee2497a5cb0236d3785c1c8cdfb 7b5ba3d2445355f386edab96125d42b7 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE) |
repository.mail.fl_str_mv |
bibliotecatede@uninove.br||bibliotecatede@uninove.br |
_version_ |
1811016868342792192 |