Efeito da eritorsina na terapia fotodinâmica antimicrobiana em cândida albicans
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/2777 |
Resumo: | Candida albicans (C. albicans) is a pseudofilamentous dimorphic fungus that can survive and infect humans with difficult resolution by antifungal therapy. Antimicrobial photodynamic therapy (aPDT) generates reactive oxygen species with the potential to kill microorganisms. The erythrosine dye has photodynamic potential and can act on oral microorganisms; therefore, the aim of this work was to investigate the effect of erythrosine-mediated aPDT and blue light on Candida albicans. The cell death time and the incorporation of erythrosine into the fungal cell were evaluated. For the execution of this work, the Candida albicans ATCC 90028 strain was selected, properly prepared for the irradiation test. Irradiation was carried out with a LED dental light-curing device (DMC, São Carlos, SP, Brazil), emitting a wavelength at ʎ = 430 nm – 490 nm and a radiant power of 1000 mW. The samples were irradiated from the top of a 24-well microtiter plate with an irradiance of I = 500 mW / cm2 and radiant exposure of 0 to 377 J/cm2, with irradiation times ranging from 0 to 720 s. The photosensitizer used was Erythrosine at concentrations of 100 μM. Yeast suspensions were divided into groups: Control without irradiation and photosensitizer (L-FS-), irradiated group without photosensitizer (L+720FS-), photosensitizer group without irradiation (L-FS+), and groups that received photosensitizer and irradiation called aPDT groups and described by the acronym L+ followed by the irradiation time in seconds and FS+. To assess the absorption and incorporation of erythrosine, fluorescence microscopy and optical absorption spectroscopy were performed. Erythrosine eradicated the tested microorganisms with total inactivation after 120 s of irradiation and incorporation of photosensitizer into the fungus was observed. APDT mediated by erythrosine and blue light was effective in inactivating Candida albicans. |
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Prates, Renato Araujohttp://lattes.cnpq.br/7664790931310514Prates, Renato Araujohttp://lattes.cnpq.br/7664790931310514Motta, Lara Jansiskihttp://lattes.cnpq.br/6750462252900819Baptista, AlessandraMarcos, Rodrigo Labathttp://lattes.cnpq.br/2964112163504080Gonçalves, Marcela Leticia Lealhttp://lattes.cnpq.br/3368360911943035http://lattes.cnpq.br/0355526757464997Rossi, Adriana Leal2021-11-25T17:39:27Z2021-10-14Rossi, Adriana Leal. Efeito da eritorsina na terapia fotodinâmica antimicrobiana em cândida albicans. 2021. 39 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo.http://bibliotecatede.uninove.br/handle/tede/2777Candida albicans (C. albicans) is a pseudofilamentous dimorphic fungus that can survive and infect humans with difficult resolution by antifungal therapy. Antimicrobial photodynamic therapy (aPDT) generates reactive oxygen species with the potential to kill microorganisms. The erythrosine dye has photodynamic potential and can act on oral microorganisms; therefore, the aim of this work was to investigate the effect of erythrosine-mediated aPDT and blue light on Candida albicans. The cell death time and the incorporation of erythrosine into the fungal cell were evaluated. For the execution of this work, the Candida albicans ATCC 90028 strain was selected, properly prepared for the irradiation test. Irradiation was carried out with a LED dental light-curing device (DMC, São Carlos, SP, Brazil), emitting a wavelength at ʎ = 430 nm – 490 nm and a radiant power of 1000 mW. The samples were irradiated from the top of a 24-well microtiter plate with an irradiance of I = 500 mW / cm2 and radiant exposure of 0 to 377 J/cm2, with irradiation times ranging from 0 to 720 s. The photosensitizer used was Erythrosine at concentrations of 100 μM. Yeast suspensions were divided into groups: Control without irradiation and photosensitizer (L-FS-), irradiated group without photosensitizer (L+720FS-), photosensitizer group without irradiation (L-FS+), and groups that received photosensitizer and irradiation called aPDT groups and described by the acronym L+ followed by the irradiation time in seconds and FS+. To assess the absorption and incorporation of erythrosine, fluorescence microscopy and optical absorption spectroscopy were performed. Erythrosine eradicated the tested microorganisms with total inactivation after 120 s of irradiation and incorporation of photosensitizer into the fungus was observed. APDT mediated by erythrosine and blue light was effective in inactivating Candida albicans.A Candida albicans (C. albicans) é um fungo dimórfico pseudofilamentar, que pode sobreviver e infectar humanos com difícil resolução por terapia antifúngica. A terapia fotodinâmica antimicrobiana (aPDT) gera espécies reativas de oxigênio com potencial para eliminação de micro-organismos. O corante eritrosina tem potencial fotodinâmico e pode ter ação em micro-organismos orais, portanto, o objetivo deste trabalho foi investigar o efeito da aPDT mediada por eritrosina e luz azul em Candida albicans. Foi avaliado o tempo de morte celular e a incorporação da eritrosina dentro da célula fúngica. Para a execução do presente trabalho foi selecionada a cepa Candida albicans ATCC 90028 devidamente preparada para o ensaio de irradiação. A irradiação foi realizada com um equipamento LED fotopolimerizador odontológico (DMC, São Carlos, SP, Brasil), emitindo comprimento de onda em ʎ = 430 nm – 490 nm e uma potência radiante de 1000 mW. As amostras foram irradiadas do topo de uma placa de microtitulação de 24 poços com irradiância de I = 500 mW / cm2 e exposição radiante de 0 a 377 J/cm2, com tempos de irradiação variando de 0 a 720 s. O fotossensibilizador utilizado foi a Eritrosina em concentrações de 100 μM. As suspensões de leveduras foram divididas em grupos: Controle sem irradiação e fotossensibilizador (L-FS-), grupo irradiado sem fotossensibilizador (L+720FS-), grupo fotossensibilizador sem irradiação (L-FS+), e os grupos que receberam fotossensibilizador e irradiação chamados grupos aPDT e descritos pela sigla L+ seguido do tempo de irradiação em segundos e FS+. Para avaliar a absorção e incorporação da eritrosina foi realizada a microscopia de fluorescência e a espectroscopia de absorção óptica. A eritrosina apresentou erradicação dos micro-organismos testados com total inativação após 120 s de irradiação e foi observado incorporação de fotossensibilizador dentro do fungo. A aPDT mediada por eritrosina e luz azul foi efetiva na inativação de Candida albicans.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2021-11-25T17:39:27Z No. of bitstreams: 1 Adriana Leal Rossi.pdf: 602732 bytes, checksum: 3d3b37c00381fdfe7b41247d0361266a (MD5)Made available in DSpace on 2021-11-25T17:39:27Z (GMT). No. of bitstreams: 1 Adriana Leal Rossi.pdf: 602732 bytes, checksum: 3d3b37c00381fdfe7b41247d0361266a (MD5) Previous issue date: 2021-10-14application/pdfporUniversidade Nove de JulhoPrograma de Pós-Graduação em Biofotônica Aplicada às Ciências da SaúdeUNINOVEBrasilSaúdecandida albicansterapia fotodinâmica antimicrobianaeritrosinaLEDmicroscopia de fluorescênciaquimioterapia fotodinâmica antimicrobiana (PACT)candida albicansantimicrobial photodynamic therapyerythrosinLEDfluorescence microscopyPhotodynamic Antimicrobial Chemotherapy PACTCIENCIAS DA SAUDEEfeito da eritorsina na terapia fotodinâmica antimicrobiana em cândida albicansEffect of erythorsin in antimicrobial photodynamic therapy in candida albicansinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALAdriana Leal Rossi.pdfAdriana Leal Rossi.pdfapplication/pdf602732http://localhost:8080/tede/bitstream/tede/2777/2/Adriana+Leal+Rossi.pdf3d3b37c00381fdfe7b41247d0361266aMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/2777/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/27772021-11-25 15:39:27.403oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2021-11-25T17:39:27Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito da eritorsina na terapia fotodinâmica antimicrobiana em cândida albicans |
dc.title.alternative.eng.fl_str_mv |
Effect of erythorsin in antimicrobial photodynamic therapy in candida albicans |
title |
Efeito da eritorsina na terapia fotodinâmica antimicrobiana em cândida albicans |
spellingShingle |
Efeito da eritorsina na terapia fotodinâmica antimicrobiana em cândida albicans Rossi, Adriana Leal candida albicans terapia fotodinâmica antimicrobiana eritrosina LED microscopia de fluorescência quimioterapia fotodinâmica antimicrobiana (PACT) candida albicans antimicrobial photodynamic therapy erythrosin LED fluorescence microscopy Photodynamic Antimicrobial Chemotherapy PACT CIENCIAS DA SAUDE |
title_short |
Efeito da eritorsina na terapia fotodinâmica antimicrobiana em cândida albicans |
title_full |
Efeito da eritorsina na terapia fotodinâmica antimicrobiana em cândida albicans |
title_fullStr |
Efeito da eritorsina na terapia fotodinâmica antimicrobiana em cândida albicans |
title_full_unstemmed |
Efeito da eritorsina na terapia fotodinâmica antimicrobiana em cândida albicans |
title_sort |
Efeito da eritorsina na terapia fotodinâmica antimicrobiana em cândida albicans |
author |
Rossi, Adriana Leal |
author_facet |
Rossi, Adriana Leal |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Prates, Renato Araujo |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7664790931310514 |
dc.contributor.referee1.fl_str_mv |
Prates, Renato Araujo |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7664790931310514 |
dc.contributor.referee2.fl_str_mv |
Motta, Lara Jansiski |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/6750462252900819 |
dc.contributor.referee3.fl_str_mv |
Baptista, Alessandra |
dc.contributor.referee4.fl_str_mv |
Marcos, Rodrigo Labat |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/2964112163504080 |
dc.contributor.referee5.fl_str_mv |
Gonçalves, Marcela Leticia Leal |
dc.contributor.referee5Lattes.fl_str_mv |
http://lattes.cnpq.br/3368360911943035 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0355526757464997 |
dc.contributor.author.fl_str_mv |
Rossi, Adriana Leal |
contributor_str_mv |
Prates, Renato Araujo Prates, Renato Araujo Motta, Lara Jansiski Baptista, Alessandra Marcos, Rodrigo Labat Gonçalves, Marcela Leticia Leal |
dc.subject.por.fl_str_mv |
candida albicans terapia fotodinâmica antimicrobiana eritrosina LED microscopia de fluorescência quimioterapia fotodinâmica antimicrobiana (PACT) |
topic |
candida albicans terapia fotodinâmica antimicrobiana eritrosina LED microscopia de fluorescência quimioterapia fotodinâmica antimicrobiana (PACT) candida albicans antimicrobial photodynamic therapy erythrosin LED fluorescence microscopy Photodynamic Antimicrobial Chemotherapy PACT CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
candida albicans antimicrobial photodynamic therapy erythrosin LED fluorescence microscopy Photodynamic Antimicrobial Chemotherapy PACT |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Candida albicans (C. albicans) is a pseudofilamentous dimorphic fungus that can survive and infect humans with difficult resolution by antifungal therapy. Antimicrobial photodynamic therapy (aPDT) generates reactive oxygen species with the potential to kill microorganisms. The erythrosine dye has photodynamic potential and can act on oral microorganisms; therefore, the aim of this work was to investigate the effect of erythrosine-mediated aPDT and blue light on Candida albicans. The cell death time and the incorporation of erythrosine into the fungal cell were evaluated. For the execution of this work, the Candida albicans ATCC 90028 strain was selected, properly prepared for the irradiation test. Irradiation was carried out with a LED dental light-curing device (DMC, São Carlos, SP, Brazil), emitting a wavelength at ʎ = 430 nm – 490 nm and a radiant power of 1000 mW. The samples were irradiated from the top of a 24-well microtiter plate with an irradiance of I = 500 mW / cm2 and radiant exposure of 0 to 377 J/cm2, with irradiation times ranging from 0 to 720 s. The photosensitizer used was Erythrosine at concentrations of 100 μM. Yeast suspensions were divided into groups: Control without irradiation and photosensitizer (L-FS-), irradiated group without photosensitizer (L+720FS-), photosensitizer group without irradiation (L-FS+), and groups that received photosensitizer and irradiation called aPDT groups and described by the acronym L+ followed by the irradiation time in seconds and FS+. To assess the absorption and incorporation of erythrosine, fluorescence microscopy and optical absorption spectroscopy were performed. Erythrosine eradicated the tested microorganisms with total inactivation after 120 s of irradiation and incorporation of photosensitizer into the fungus was observed. APDT mediated by erythrosine and blue light was effective in inactivating Candida albicans. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-11-25T17:39:27Z |
dc.date.issued.fl_str_mv |
2021-10-14 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Rossi, Adriana Leal. Efeito da eritorsina na terapia fotodinâmica antimicrobiana em cândida albicans. 2021. 39 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/2777 |
identifier_str_mv |
Rossi, Adriana Leal. Efeito da eritorsina na terapia fotodinâmica antimicrobiana em cândida albicans. 2021. 39 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo. |
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http://bibliotecatede.uninove.br/handle/tede/2777 |
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por |
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