Expressão imunoistoquímica das enzimas DNA metiltransferases 1, 3A e 3B em leucoplasias e carcinomas de células escamosas bucais
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional PUCRS |
Texto Completo: | http://hdl.handle.net/10923/369 |
Resumo: | Objectives: This study investigated the expression of DNA methyltransferase (DNMT) 1, 3a, and 3b enzymes in oral squamous cell carcinoma (SCC) and leukoplakia, their relationship with histopathologic graduation and dysplasia, respectively, and with clinical parameters. Study design: Immunohistochemistry using antibodies anti-DNMT1, anti-DNMT3a, and anti-DNMT3b (dilution of 1:700, Imgenex, San Diego, USA) was carried out to detect the expression of the 3 DNMTs proteins in 21 oral leukoplakias with histopathologic diagnosis of acanthosis and/or hyperkeratosis (leukoplakia without dysplasia), 16 leukoplakias with histopathologic diagnosis of epithelial dysplasia, 20 oral well differentiated SCC (grade I), 20 oral moderately differentiated SCC (grade II), and 20 oral poorly differentiated SCC (grade III). Twenty samples of non-tumor oral tissues were obtained as control. The tissues were embedded in paraffin and obtained from the pathologic archive of Division of Stomatology and Prevention of Bucomaxillofacial Cancer, São Lucas Hospital, PUCRS. Statistical analysis was done using Analysis of Variance (ANOVA), Student-Newmann-Keuls test, Pearson correlation and t test. Results: The incidence of nuclear DNMT3a immunoreactivity in oral SCC groups (39. 9%) was significantly higher than in control (22. 6%) (P<0. 05), but not when compared to oral leukoplakias groups (28. 2%). For DNMT1 and DNMT3b, there were no statistically significant difference between oral SCC groups (65% and 74. 7%), oral leukoplakia groups (68. 3% and 70. 9%) and control (65. 4% and 76. 5%). DNMT protein expression exhibited no correlation with age and no association with gender and mate. There were a significant association between the DNMT3a and alcohol use (P = 0. 01), and an inverse association between DNMT1 and smoking (P = 0. 048).Conclusions: Although there was a higher level of DNMT3a immunopositivity in SCC groups, the three studied enzymes had no predictive capacity for SCC development in patients with oral leukoplakia, and exhibited no association with their histopathological features or the clinical parameters age, gender and mate consumption. Alcohol intake was associated with a higher DNMT3a expression and smoking with a lower DNMT1 expression. |
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Daniel, Filipe IvanSalum, Fernando Antônio2011-12-27T14:14:02Z2011-12-27T14:14:02Z2009http://hdl.handle.net/10923/369Objectives: This study investigated the expression of DNA methyltransferase (DNMT) 1, 3a, and 3b enzymes in oral squamous cell carcinoma (SCC) and leukoplakia, their relationship with histopathologic graduation and dysplasia, respectively, and with clinical parameters. Study design: Immunohistochemistry using antibodies anti-DNMT1, anti-DNMT3a, and anti-DNMT3b (dilution of 1:700, Imgenex, San Diego, USA) was carried out to detect the expression of the 3 DNMTs proteins in 21 oral leukoplakias with histopathologic diagnosis of acanthosis and/or hyperkeratosis (leukoplakia without dysplasia), 16 leukoplakias with histopathologic diagnosis of epithelial dysplasia, 20 oral well differentiated SCC (grade I), 20 oral moderately differentiated SCC (grade II), and 20 oral poorly differentiated SCC (grade III). Twenty samples of non-tumor oral tissues were obtained as control. The tissues were embedded in paraffin and obtained from the pathologic archive of Division of Stomatology and Prevention of Bucomaxillofacial Cancer, São Lucas Hospital, PUCRS. Statistical analysis was done using Analysis of Variance (ANOVA), Student-Newmann-Keuls test, Pearson correlation and t test. Results: The incidence of nuclear DNMT3a immunoreactivity in oral SCC groups (39. 9%) was significantly higher than in control (22. 6%) (P<0. 05), but not when compared to oral leukoplakias groups (28. 2%). For DNMT1 and DNMT3b, there were no statistically significant difference between oral SCC groups (65% and 74. 7%), oral leukoplakia groups (68. 3% and 70. 9%) and control (65. 4% and 76. 5%). DNMT protein expression exhibited no correlation with age and no association with gender and mate. There were a significant association between the DNMT3a and alcohol use (P = 0. 01), and an inverse association between DNMT1 and smoking (P = 0. 048).Conclusions: Although there was a higher level of DNMT3a immunopositivity in SCC groups, the three studied enzymes had no predictive capacity for SCC development in patients with oral leukoplakia, and exhibited no association with their histopathological features or the clinical parameters age, gender and mate consumption. Alcohol intake was associated with a higher DNMT3a expression and smoking with a lower DNMT1 expression.Objetivos: Neste estudo foi investigada a expressão das enzimas DNA metiltransferases (DNMTs) 1, 3a e 3b em carcinomas escamocelulares (CEC) e leucoplasias bucais, relacionando-a com a graduação histopatológica e presença de displasia epitelial, respectivamente, bem como com os parâmetros clínicos dos pacientes. Metodologia: A técnica de imunoistoquímica utilizando anticorpos anti-DNMT1, anti-DNMT3a e anti-DNMT3b (diluição de 1:700) foi realizada para detectar a expressão das três DNMTs em 21 amostras de leucoplasias com diagnóstico histopatológico de acantose e/ou hiperceratose (leucoplasia sem displasia), 16 leucoplasias com diagnóstico histopatológico de displasia epitelial, 20 CEC bem diferenciados (grau I), 20 CEC moderadamente diferenciados (grau II) e 20 CEC pobremente diferenciados (grau III). Vinte amostras de tecidos orais não tumorais foram utilizadas como controles. O material, incluído em parafina, foi obtido do arquivo do Laboratório de Patologia do Serviço de Estomatologia e Prevenção do Câncer Bucomaxilofacial do Hospital São Lucas da PUCRS. A análise estatística foi realizada por meio da Análise de Variância (ANOVA), teste Student-Newman-Keuls, correlação de Pearson e teste t. Resultados: A incidência da imunorreatividade nuclear para DNMT3a nos grupos de CEC (39,9%) foi significativamente superior a do grupo-controle (22,6%) (p<0,05), mas não diferiu dos grupos de leucoplasias (28,2%). Para a DNMT1 e DNMT3b não houve diferença estatisticamente significativa entre os grupos de CEC (65% e 74,7%), de leucoplasias (68,3% e 70,9%) e controle (65,4% e 76,5%).A expressão imunoistoquímica das enzimas DNMTs não exibiu correlação com a idade dos pacientes nem associação com gênero e consumo de chimarrão. Houve uma significativa associação entre DNMT3a e uso de álcool (p = 0,01) e uma associação inversa entre DNMT1 e tabagismo (p = 0,048). Conclusões: Apesar do aumento do nível de imunorreatividade à enzima DNMT3a nos grupos de CEC, as três enzimas estudadas não possuem capacidade preditora de susceptibilidade ao CEC em pacientes com leucoplasia bucal, nem exibem associação com as características histopatológicas ou com os parâmetros clínicos idade, gênero e uso de chimarrão. O consumo de bebidas alcoólicas foi associado com uma maior expressão de DNMT3a e o uso de cigarro com uma menor expressão de DNMT1.Made available in DSpace on 2011-12-27T14:14:02Z (GMT). No. of bitstreams: 2 000419163-0.pdf: 7573936 bytes, checksum: cf99bdb6eeb8892e65fd4e0a5c3a15e2 (MD5) license.txt: 581 bytes, checksum: 44ea52f0b7567232681c6e3d72285adc (MD5)Pontifícia Universidade Católica do Rio Grande do SulPorto AlegreODONTOLOGIAESTOMATOLOGIA CLÍNICANEOPLASIAS BUCAISCARCINOMA DE CÉLULAS ESCAMOSASIMUNOISTOQUÍMICADNAExpressão imunoistoquímica das enzimas DNA metiltransferases 1, 3A e 3B em leucoplasias e carcinomas de células escamosas bucaisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisPontifícia Universidade Católica do Rio Grande do SulFaculdade de OdontologiaPrograma de Pós-Graduação em OdontologiaDoutorado2009porreponame:Repositório Institucional PUCRSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSinfo:eu-repo/semantics/openAccessORIGINAL000419163-0.pdfTexto Completoapplication/pdf7573936repositorio.pucrs.br/jspui/bitstream/10923/369/1/000419163-0.pdfcf99bdb6eeb8892e65fd4e0a5c3a15e2MD51LICENSElicense.txttext/plain581repositorio.pucrs.br/jspui/bitstream/10923/369/2/license.txt44ea52f0b7567232681c6e3d72285adcMD52TEXT000419163-0.pdf.txt000419163-0.pdf.txtExtracted texttext/plain133595repositorio.pucrs.br/jspui/bitstream/10923/369/3/000419163-0.pdf.txt5a338023cf96b7e0f6b2d3205ed9561fMD5310923/3692017-09-28 11:05:35.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ório InstitucionalPRI |
dc.title.pt_BR.fl_str_mv |
Expressão imunoistoquímica das enzimas DNA metiltransferases 1, 3A e 3B em leucoplasias e carcinomas de células escamosas bucais |
title |
Expressão imunoistoquímica das enzimas DNA metiltransferases 1, 3A e 3B em leucoplasias e carcinomas de células escamosas bucais |
spellingShingle |
Expressão imunoistoquímica das enzimas DNA metiltransferases 1, 3A e 3B em leucoplasias e carcinomas de células escamosas bucais Daniel, Filipe Ivan ODONTOLOGIA ESTOMATOLOGIA CLÍNICA NEOPLASIAS BUCAIS CARCINOMA DE CÉLULAS ESCAMOSAS IMUNOISTOQUÍMICA DNA |
title_short |
Expressão imunoistoquímica das enzimas DNA metiltransferases 1, 3A e 3B em leucoplasias e carcinomas de células escamosas bucais |
title_full |
Expressão imunoistoquímica das enzimas DNA metiltransferases 1, 3A e 3B em leucoplasias e carcinomas de células escamosas bucais |
title_fullStr |
Expressão imunoistoquímica das enzimas DNA metiltransferases 1, 3A e 3B em leucoplasias e carcinomas de células escamosas bucais |
title_full_unstemmed |
Expressão imunoistoquímica das enzimas DNA metiltransferases 1, 3A e 3B em leucoplasias e carcinomas de células escamosas bucais |
title_sort |
Expressão imunoistoquímica das enzimas DNA metiltransferases 1, 3A e 3B em leucoplasias e carcinomas de células escamosas bucais |
author |
Daniel, Filipe Ivan |
author_facet |
Daniel, Filipe Ivan |
author_role |
author |
dc.contributor.author.fl_str_mv |
Daniel, Filipe Ivan |
dc.contributor.advisor1.fl_str_mv |
Salum, Fernando Antônio |
contributor_str_mv |
Salum, Fernando Antônio |
dc.subject.por.fl_str_mv |
ODONTOLOGIA ESTOMATOLOGIA CLÍNICA NEOPLASIAS BUCAIS CARCINOMA DE CÉLULAS ESCAMOSAS IMUNOISTOQUÍMICA DNA |
topic |
ODONTOLOGIA ESTOMATOLOGIA CLÍNICA NEOPLASIAS BUCAIS CARCINOMA DE CÉLULAS ESCAMOSAS IMUNOISTOQUÍMICA DNA |
description |
Objectives: This study investigated the expression of DNA methyltransferase (DNMT) 1, 3a, and 3b enzymes in oral squamous cell carcinoma (SCC) and leukoplakia, their relationship with histopathologic graduation and dysplasia, respectively, and with clinical parameters. Study design: Immunohistochemistry using antibodies anti-DNMT1, anti-DNMT3a, and anti-DNMT3b (dilution of 1:700, Imgenex, San Diego, USA) was carried out to detect the expression of the 3 DNMTs proteins in 21 oral leukoplakias with histopathologic diagnosis of acanthosis and/or hyperkeratosis (leukoplakia without dysplasia), 16 leukoplakias with histopathologic diagnosis of epithelial dysplasia, 20 oral well differentiated SCC (grade I), 20 oral moderately differentiated SCC (grade II), and 20 oral poorly differentiated SCC (grade III). Twenty samples of non-tumor oral tissues were obtained as control. The tissues were embedded in paraffin and obtained from the pathologic archive of Division of Stomatology and Prevention of Bucomaxillofacial Cancer, São Lucas Hospital, PUCRS. Statistical analysis was done using Analysis of Variance (ANOVA), Student-Newmann-Keuls test, Pearson correlation and t test. Results: The incidence of nuclear DNMT3a immunoreactivity in oral SCC groups (39. 9%) was significantly higher than in control (22. 6%) (P<0. 05), but not when compared to oral leukoplakias groups (28. 2%). For DNMT1 and DNMT3b, there were no statistically significant difference between oral SCC groups (65% and 74. 7%), oral leukoplakia groups (68. 3% and 70. 9%) and control (65. 4% and 76. 5%). DNMT protein expression exhibited no correlation with age and no association with gender and mate. There were a significant association between the DNMT3a and alcohol use (P = 0. 01), and an inverse association between DNMT1 and smoking (P = 0. 048).Conclusions: Although there was a higher level of DNMT3a immunopositivity in SCC groups, the three studied enzymes had no predictive capacity for SCC development in patients with oral leukoplakia, and exhibited no association with their histopathological features or the clinical parameters age, gender and mate consumption. Alcohol intake was associated with a higher DNMT3a expression and smoking with a lower DNMT1 expression. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009 |
dc.date.accessioned.fl_str_mv |
2011-12-27T14:14:02Z |
dc.date.available.fl_str_mv |
2011-12-27T14:14:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10923/369 |
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http://hdl.handle.net/10923/369 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Pontifícia Universidade Católica do Rio Grande do Sul Porto Alegre |
publisher.none.fl_str_mv |
Pontifícia Universidade Católica do Rio Grande do Sul Porto Alegre |
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Repositório Institucional PUCRS |
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