Ensaio de citotoxicidade do ácido hialurônico como veículo no composto celular autógeno para enxertia óssea

• Autor(a) principal: Boeckel, Daniel Gonçalves
• Data de Publicação: 2011
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional PUCRS
• Texto Completo: http://hdl.handle.net/10923/396
• topic: ODONTOLOGIA; MATERIAIS DENTÁRIOS; ÁCIDO HIALURÔNICO; REGENERAÇÃO ÓSSEA; TRANSPLANTE ÓSSEO

Tissue engineering, using pluripotent cells, biocompatible matrixes and growth factors, may play a major role in bone regeneration. Through living tissue biopsy, it is possible to obtain mesenchymal stem cells that, when expanded and cultivated in vitro, can generate several types of tissues. Thus, it is possible to present an alternative to bone grafts, since the amount of material collected is smaller when compared to conventional bone grafts. The present research aimed to find a better biocompatible matrix, evaluating, in vitro, the cytotoxicity of TEOSYAL® hyaluronic acid directly onto pre-osteoblastic lineage mouse cells denominated OFCOL II, in a quantitative and qualitative way. The following experimental groups have been formed: A) Cells + Hyaluronic Acid (HA) + Platelet Rich Plasma with thrombin (PRP) + Hydroxyapatite (HP); B) Cells + HA + PRP + HP; C) Cells + HA + HP; D) Cells + HP; E) Cells + HA; F) Cells + PRP with thrombin; G) Cells + PRP and H) Pure DMEM with 15% of Fetal Bovine Serum (FBS), used as a positive control. The OFCOL II cells were seeded 24 hours before the experiments and incubated at 37ºC with 5% CO2. After reaching the semi-confluence, the cells were exposed to the experimental groups for an additional period of 48 hours under the same temperature and humidity conditions. The cellular viability was evaluated through MTT test and compared to the positive control group H. The numerical values, obtained in absorbance, were subjected to statistical analysis of variance (ANOVA), complemented by TUKEY test, with significance level p < 0. 05 for comparison between all groups. As for comparisons between the control and each experimental group, the T test was used. In the cell morphology evaluation, it was used optical microscopy at magnification 100x. The results proved a decrease in cell viability in groups B (67% viability) and C (68% viability), differing from group D (99% viability). When comparing each experimental group to the positive control group, a statistical difference (p < 0. 05) was obtained for groups B, C and E. Therefore, through these results, it was observed a decrease in cellular viability in the groups with the presence of TEOSYAL® hyaluronic acid. The addition of PRP to hyaluronic acid improved cell viability since it was possible to obtain a survival rate of 79% (group A), although this stimulus was insufficient to enhance it. Regarding image analysis, morphological changes were found in experimental groups and control group. It is possible to conclude that the presence of TEOSYAL® hyaluronic acid exerted a decrease effect on the cell lineage OFCOL II viability.