Efeito do Agregado Trióxido Mineral intracanal em um modelo de reimplante tardio em dentes de ratos: análise histológica e imunoistoquímica

Detalhes bibliográficos
Autor(a) principal: Vogt, Beatriz Farias
Data de Publicação: 2011
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional PUCRS
Texto Completo: http://hdl.handle.net/10923/437
Resumo: The replantation is the treatment of choice for tooth avulsion. It should be performed immediately, in order to avoid the occurrence of pulp and periodontal ligament necrosis, culminating in root resorption development. However, when resorption is present, calcium hydroxide therapy should be initiated as soon as possible. Other materials have been tested as substitutes for calcium hydroxide. Therefore, the aim of this study was to evaluate the effect of White MTA (WMTA) and MTA Fillapex as intracanal medications to prevent root resorption, in a rat model of delayed tooth raplantation. Maxillary right central incisors of 48 male rats were extracted, and left exposed to dry environment for 30 min. The animals were allocated into four groups: 1 – WMTA; 2 – MTA Fillapex; 3 – Calcium hydroxide; 4 - Negative control. Following periodontal ligament removal, root canals were dried and filled with the corresponding material for each group and replanted. After 10 and 60 days, all surgical specimens were subjected to histological processing. The slides were stained with HE for histological qualitative and quantitative analysis. The results were statistically analyzed by ANOVA followed by Tukey’s post-hoc test, with a significance level of 5%. Qualitative evaluation revealed that both WMTA and MTA Fillapex groups showed inflammatory and replacement resorption and dento-alveolar ankylosis profiles, which were similar to that observed for calcium hydroxide, in both time-points of evaluation. A slight increase of the inflammatory process was observed in either MTA groups. Quantitatively, inflammation score analysis showed a significant difference between the calcium hydroxide and the control group at 10 days (p<0. 05). On 60 days, dento-alveolar ankylosis was found significantly increased in the MTA Fillapex, in comparison to the control group (p<0. 05). Our data showed that none of tested materials was able to fully prevent the root resorption, although either MTA-based intracanal medications presented an outcome comparable to that seen for calcium hydroxide. Therefore, MTA cements might present some advantages, but the consequences of the pro-inflammatory effects of MTA cements need to be further investigated.
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spelling Vogt, Beatriz FariasCampos, Maria Martha2011-12-27T14:14:14Z2011-12-27T14:14:14Z2011http://hdl.handle.net/10923/437The replantation is the treatment of choice for tooth avulsion. It should be performed immediately, in order to avoid the occurrence of pulp and periodontal ligament necrosis, culminating in root resorption development. However, when resorption is present, calcium hydroxide therapy should be initiated as soon as possible. Other materials have been tested as substitutes for calcium hydroxide. Therefore, the aim of this study was to evaluate the effect of White MTA (WMTA) and MTA Fillapex as intracanal medications to prevent root resorption, in a rat model of delayed tooth raplantation. Maxillary right central incisors of 48 male rats were extracted, and left exposed to dry environment for 30 min. The animals were allocated into four groups: 1 – WMTA; 2 – MTA Fillapex; 3 – Calcium hydroxide; 4 - Negative control. Following periodontal ligament removal, root canals were dried and filled with the corresponding material for each group and replanted. After 10 and 60 days, all surgical specimens were subjected to histological processing. The slides were stained with HE for histological qualitative and quantitative analysis. The results were statistically analyzed by ANOVA followed by Tukey’s post-hoc test, with a significance level of 5%. Qualitative evaluation revealed that both WMTA and MTA Fillapex groups showed inflammatory and replacement resorption and dento-alveolar ankylosis profiles, which were similar to that observed for calcium hydroxide, in both time-points of evaluation. A slight increase of the inflammatory process was observed in either MTA groups. Quantitatively, inflammation score analysis showed a significant difference between the calcium hydroxide and the control group at 10 days (p<0. 05). On 60 days, dento-alveolar ankylosis was found significantly increased in the MTA Fillapex, in comparison to the control group (p<0. 05). Our data showed that none of tested materials was able to fully prevent the root resorption, although either MTA-based intracanal medications presented an outcome comparable to that seen for calcium hydroxide. Therefore, MTA cements might present some advantages, but the consequences of the pro-inflammatory effects of MTA cements need to be further investigated.O reimplante é o tratamento de escolha da avulsão dentária. Deve ser realizado imediatamente, a fim de evitar que ocorra necrose pulpar e do ligamento periodontal e, consequentemente, o desenvolvimento de reabsorção radicular. Porém, quando a reabsorção estiver instalada, a terapia com hidróxido de cálcio deve ser iniciada tão logo quanto possível. Pelo fato deste material ainda apresentar alguns inconvenientes, como a necessidade de trocas frequentes e o aumento da fragilidade da estrutura dentária quando utilizado por longos períodos, outros materiais estão sendo utilizados como substitutos do hidróxido de cálcio. Sendo assim, o objetivo deste trabalho foi de, através de uma revisão da literatura, conhecer a aplicabilidade do agregado trióxido mineral no tratamento de dentes traumatizados. Além disso, também foi realizado um estudo experimental para avaliar o efeito do MTA Branco (WMTA) (Angelus, Londrina, Brasil) e do MTA Fillapex (Angelus, Londrina Brasil) como medicação intracanal na prevenção da reabsorção radicular, em dentes de ratos, reimplantados tardiamente. Neste estudo foram utilizados 48 ratos machos (Wistar) que tiveram o incisivo central superior direito extraído e mantido em meio seco por 30 minutos. Os animais foram divididos em quatro grupos: 1 – WMTA; 2 – MTA Fillapex; 3 – Hidróxido de cálcio; 4 – Controle negativo. Após a remoção química do ligamento periodontal, os canais radiculares foram secos, preenchidos com o material correspondente a cada grupo e reimplantados. Após 10 e 60 dias, as peças operatórias foram submetidas ao processamento para realização de cortes histológicos no sentido longitudinal. As lâminas foram coradas com HE para análise histológica qualitativa e quantitativa. Os resultados foram analisados estatisticamente por meio de análise de variância (ANOVA), seguida pelo Teste de Tukey, com nível de significância de 5%. A avaliação qualitativa demonstrou que os grupos do WMTA e MTA Fillapex apresentaram áreas de reabsorção inflamatória, reabsorção por substituição e anquilose alvéolo-dentária, similares às observadas no grupo do hidróxido de cálcio, nos dois períodos avaliados. Um processo inflamatório maior foi observado nos grupos WMTA e MTA Fillapex. Na análise quantitativa, a avaliação do processo inflamatório mostrou diferença significativa entre o grupo do hidróxido de cálcio e o grupo controle, aos 10 dias (p<0. 05). Aos 60 dias, áreas de anquilose alvéolodentária foram significativamente maiores no grupo MTA Fillapex quando comparado ao grupo controle (p<0. 05). Os resultados mostraram que nenhum dos materiais avaliados foi capaz de prevenir completamente o desenvolvimento da reabsorção radicular, apesar de ambos os materiais à base de MTA terem apresentado resultados comparáveis aos do hidróxido de cálcio. Sendo assim, os cimentos de MTA podem apresentar algumas vantagens; porém, as consequências dos seus efeitos pro-inflamatórios devem ser mais bem investigadas.Made available in DSpace on 2011-12-27T14:14:14Z (GMT). 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dc.title.pt_BR.fl_str_mv Efeito do Agregado Trióxido Mineral intracanal em um modelo de reimplante tardio em dentes de ratos: análise histológica e imunoistoquímica
title Efeito do Agregado Trióxido Mineral intracanal em um modelo de reimplante tardio em dentes de ratos: análise histológica e imunoistoquímica
spellingShingle Efeito do Agregado Trióxido Mineral intracanal em um modelo de reimplante tardio em dentes de ratos: análise histológica e imunoistoquímica
Vogt, Beatriz Farias
ODONTOLOGIA
REIMPLANTE DENTÁRIO
AVULSÃO DENTÁRIA
IMUNOISTOQUÍMICA
title_short Efeito do Agregado Trióxido Mineral intracanal em um modelo de reimplante tardio em dentes de ratos: análise histológica e imunoistoquímica
title_full Efeito do Agregado Trióxido Mineral intracanal em um modelo de reimplante tardio em dentes de ratos: análise histológica e imunoistoquímica
title_fullStr Efeito do Agregado Trióxido Mineral intracanal em um modelo de reimplante tardio em dentes de ratos: análise histológica e imunoistoquímica
title_full_unstemmed Efeito do Agregado Trióxido Mineral intracanal em um modelo de reimplante tardio em dentes de ratos: análise histológica e imunoistoquímica
title_sort Efeito do Agregado Trióxido Mineral intracanal em um modelo de reimplante tardio em dentes de ratos: análise histológica e imunoistoquímica
author Vogt, Beatriz Farias
author_facet Vogt, Beatriz Farias
author_role author
dc.contributor.author.fl_str_mv Vogt, Beatriz Farias
dc.contributor.advisor1.fl_str_mv Campos, Maria Martha
contributor_str_mv Campos, Maria Martha
dc.subject.por.fl_str_mv ODONTOLOGIA
REIMPLANTE DENTÁRIO
AVULSÃO DENTÁRIA
IMUNOISTOQUÍMICA
topic ODONTOLOGIA
REIMPLANTE DENTÁRIO
AVULSÃO DENTÁRIA
IMUNOISTOQUÍMICA
description The replantation is the treatment of choice for tooth avulsion. It should be performed immediately, in order to avoid the occurrence of pulp and periodontal ligament necrosis, culminating in root resorption development. However, when resorption is present, calcium hydroxide therapy should be initiated as soon as possible. Other materials have been tested as substitutes for calcium hydroxide. Therefore, the aim of this study was to evaluate the effect of White MTA (WMTA) and MTA Fillapex as intracanal medications to prevent root resorption, in a rat model of delayed tooth raplantation. Maxillary right central incisors of 48 male rats were extracted, and left exposed to dry environment for 30 min. The animals were allocated into four groups: 1 – WMTA; 2 – MTA Fillapex; 3 – Calcium hydroxide; 4 - Negative control. Following periodontal ligament removal, root canals were dried and filled with the corresponding material for each group and replanted. After 10 and 60 days, all surgical specimens were subjected to histological processing. The slides were stained with HE for histological qualitative and quantitative analysis. The results were statistically analyzed by ANOVA followed by Tukey’s post-hoc test, with a significance level of 5%. Qualitative evaluation revealed that both WMTA and MTA Fillapex groups showed inflammatory and replacement resorption and dento-alveolar ankylosis profiles, which were similar to that observed for calcium hydroxide, in both time-points of evaluation. A slight increase of the inflammatory process was observed in either MTA groups. Quantitatively, inflammation score analysis showed a significant difference between the calcium hydroxide and the control group at 10 days (p<0. 05). On 60 days, dento-alveolar ankylosis was found significantly increased in the MTA Fillapex, in comparison to the control group (p<0. 05). Our data showed that none of tested materials was able to fully prevent the root resorption, although either MTA-based intracanal medications presented an outcome comparable to that seen for calcium hydroxide. Therefore, MTA cements might present some advantages, but the consequences of the pro-inflammatory effects of MTA cements need to be further investigated.
publishDate 2011
dc.date.accessioned.fl_str_mv 2011-12-27T14:14:14Z
dc.date.available.fl_str_mv 2011-12-27T14:14:14Z
dc.date.issued.fl_str_mv 2011
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dc.publisher.none.fl_str_mv Pontifícia Universidade Católica do Rio Grande do Sul
Porto Alegre
publisher.none.fl_str_mv Pontifícia Universidade Católica do Rio Grande do Sul
Porto Alegre
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