Citotoxicidade e genotoxicidade de materiais ortodônticos
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional PUCRS |
Texto Completo: | http://hdl.handle.net/10923/370 |
Resumo: | Biocompatibility evaluation of orthodontic materials is very important, since some constituent elements of these materials are related to citotoxic, genotoxic and carcinogenic effects. This study aimed to assess citotoxicity and genotoxicity of orthodontic materials. Citotoxicity of orthodontic materials (brackets, wires, resin composites, solder silver and elastomers) was tested by two survival exposure to Saccharomyces cerevisiae (S. Cerevisiae), through direct and indirect (chemical products liberated by these materials in artificial saliva) forms (Article 1). Assessment of genotoxicity was carried out in patient groups, using the following tests: Cytome buccal micronucleus assay for evaluating orthodontic fixed appliances and Hyrax (Article 2), Micronucleus tests for Hyrax and Haas appliances (Article 3). For Article 1, occurrence of S. Cerevisiae survival was compared in a semi-log curve between orthodontic materials and control groups. For articles 2 and 3, non parametric test was used (ES0. 05%) for comparison among patients and control groups. Silver solder was the only material that showed citotoxicity. All appliances tested did not present genotoxicity in studied patients. |
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Westphalen, Graziela HenriquesMenezes, Luciane Macedo de2011-12-27T14:14:02Z2011-12-27T14:14:02Z2009http://hdl.handle.net/10923/370Biocompatibility evaluation of orthodontic materials is very important, since some constituent elements of these materials are related to citotoxic, genotoxic and carcinogenic effects. This study aimed to assess citotoxicity and genotoxicity of orthodontic materials. Citotoxicity of orthodontic materials (brackets, wires, resin composites, solder silver and elastomers) was tested by two survival exposure to Saccharomyces cerevisiae (S. Cerevisiae), through direct and indirect (chemical products liberated by these materials in artificial saliva) forms (Article 1). Assessment of genotoxicity was carried out in patient groups, using the following tests: Cytome buccal micronucleus assay for evaluating orthodontic fixed appliances and Hyrax (Article 2), Micronucleus tests for Hyrax and Haas appliances (Article 3). For Article 1, occurrence of S. Cerevisiae survival was compared in a semi-log curve between orthodontic materials and control groups. For articles 2 and 3, non parametric test was used (ES0. 05%) for comparison among patients and control groups. Silver solder was the only material that showed citotoxicity. All appliances tested did not present genotoxicity in studied patients.A avaliação da biocompatibilidade dos materiais ortodônticos é de extrema importância, uma vez que elementos constituintes desses materiais estão relacionados a efeitos citotóxicos, genotóxicos e carcinogênicos Este estudo objetivou verificar a citotoxicidade e genotoxicidade de materiais ortodônticos. A citotoxicidade de materiais ortodônticos (bráquetes, fios, resinas compostas, soldas de prata e elásticos) foi testada por meio de duas exposições de sobrevivência ao Saccharomyces cerevisiae (S. Cerevisiae) de forma direta e indireta (produtos químicos liberados pelos materiais em saliva artificial) (Artigo 1). A avaliação da genotoxicidade foi realizada em grupos de pacientes, empregando-se os testes: Ensaio citoma bucal de micronúcleos para avaliação de aparelhos ortodônticos fixos e Hyrax (Artigo 2); Teste de micronúcleos para aparelhos expansores maxilares de Haas e Hyrax (Artigo 3). No artigo 1 foram realizadas comparações de ocorrência de sobrevivência de S. Cerevisiae em curvas semi-log entre os materiais ortodônticos e controle. Já nos artigos 2 e 3 foram empregados testes estatísticos não paramétricos diferentes (ES0,05) para comparações entre grupos de pacientes e controle. Foi observado que a solda de prata foi o único material que apresentou citotoxicidade. Todos os aparelhos testados não mostraram genotoxicidade nos grupos de pacientes estudados.Made available in DSpace on 2011-12-27T14:14:02Z (GMT). No. of bitstreams: 2 000419165-0.pdf: 5355760 bytes, checksum: 19b3455362048d8c78c84e65df0785d8 (MD5) license.txt: 581 bytes, checksum: 44ea52f0b7567232681c6e3d72285adc (MD5)Pontifícia Universidade Católica do Rio Grande do SulPorto AlegreODONTOLOGIAORTODONTIAORTOPEDIA FACIALMATERIAIS DENTÁRIOSTOXICIDADECitotoxicidade e genotoxicidade de materiais ortodônticosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisPontifícia Universidade Católica do Rio Grande do SulFaculdade de OdontologiaPrograma de Pós-Graduação em OdontologiaDoutorado2009porreponame:Repositório Institucional PUCRSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSinfo:eu-repo/semantics/openAccessORIGINAL000419165-0.pdfTexto Completoapplication/pdf5355760repositorio.pucrs.br/jspui/bitstream/10923/370/1/000419165-0.pdf19b3455362048d8c78c84e65df0785d8MD51LICENSElicense.txttext/plain581repositorio.pucrs.br/jspui/bitstream/10923/370/2/license.txt44ea52f0b7567232681c6e3d72285adcMD52TEXT000419165-0.pdf.txt000419165-0.pdf.txtExtracted texttext/plain95803repositorio.pucrs.br/jspui/bitstream/10923/370/3/000419165-0.pdf.txtf7b6aaf272b8df56145f69d7510baf21MD5310923/3702017-09-28 11:05:34.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ório InstitucionalPRI |
dc.title.pt_BR.fl_str_mv |
Citotoxicidade e genotoxicidade de materiais ortodônticos |
title |
Citotoxicidade e genotoxicidade de materiais ortodônticos |
spellingShingle |
Citotoxicidade e genotoxicidade de materiais ortodônticos Westphalen, Graziela Henriques ODONTOLOGIA ORTODONTIA ORTOPEDIA FACIAL MATERIAIS DENTÁRIOS TOXICIDADE |
title_short |
Citotoxicidade e genotoxicidade de materiais ortodônticos |
title_full |
Citotoxicidade e genotoxicidade de materiais ortodônticos |
title_fullStr |
Citotoxicidade e genotoxicidade de materiais ortodônticos |
title_full_unstemmed |
Citotoxicidade e genotoxicidade de materiais ortodônticos |
title_sort |
Citotoxicidade e genotoxicidade de materiais ortodônticos |
author |
Westphalen, Graziela Henriques |
author_facet |
Westphalen, Graziela Henriques |
author_role |
author |
dc.contributor.author.fl_str_mv |
Westphalen, Graziela Henriques |
dc.contributor.advisor1.fl_str_mv |
Menezes, Luciane Macedo de |
contributor_str_mv |
Menezes, Luciane Macedo de |
dc.subject.por.fl_str_mv |
ODONTOLOGIA ORTODONTIA ORTOPEDIA FACIAL MATERIAIS DENTÁRIOS TOXICIDADE |
topic |
ODONTOLOGIA ORTODONTIA ORTOPEDIA FACIAL MATERIAIS DENTÁRIOS TOXICIDADE |
description |
Biocompatibility evaluation of orthodontic materials is very important, since some constituent elements of these materials are related to citotoxic, genotoxic and carcinogenic effects. This study aimed to assess citotoxicity and genotoxicity of orthodontic materials. Citotoxicity of orthodontic materials (brackets, wires, resin composites, solder silver and elastomers) was tested by two survival exposure to Saccharomyces cerevisiae (S. Cerevisiae), through direct and indirect (chemical products liberated by these materials in artificial saliva) forms (Article 1). Assessment of genotoxicity was carried out in patient groups, using the following tests: Cytome buccal micronucleus assay for evaluating orthodontic fixed appliances and Hyrax (Article 2), Micronucleus tests for Hyrax and Haas appliances (Article 3). For Article 1, occurrence of S. Cerevisiae survival was compared in a semi-log curve between orthodontic materials and control groups. For articles 2 and 3, non parametric test was used (ES0. 05%) for comparison among patients and control groups. Silver solder was the only material that showed citotoxicity. All appliances tested did not present genotoxicity in studied patients. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009 |
dc.date.accessioned.fl_str_mv |
2011-12-27T14:14:02Z |
dc.date.available.fl_str_mv |
2011-12-27T14:14:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
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http://hdl.handle.net/10923/370 |
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por |
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Pontifícia Universidade Católica do Rio Grande do Sul Porto Alegre |
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Pontifícia Universidade Católica do Rio Grande do Sul Porto Alegre |
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