Prevention of oxidative stress in renal ischemia-reperfusion syndrome in rats with nutritional antioxidant supplementation

Detalhes bibliográficos
Autor(a) principal: PERCÁRIO, Sandro
Data de Publicação: 2023
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista de Nutrição
Texto Completo: https://periodicos.puc-campinas.edu.br/nutricao/article/view/9358
Resumo: ObjectiveThe objective of this study was to verify the potential protective effect of antioxidant vitamin supplementation in a model of renal ischemic-reperfusion injury in rats. MethodsTwenty-nine Wistar rats were divided into three groups: groups I and II (n=10 each), were submitted to 60 minutes of renal ischemia, followed by 10 minutes of reperfusion; additionally, animals of group II were treated for twelve days with antioxidant vitamins (vitamin C 11.43mg/kg and vitamin E 28.5 mg/kg) before being submitted to ischemia; In group III (n=9), the animals were treated like the other groups but not submitted to ischemic-reperfusion injury and not given antioxidant supplements. Subsequently, blood samples and the kidneys were collected for assessment of malondialdehyde, uric acid and total antioxidant capacity. ResultsThe malondialdehyde and uric acid of group I was significantly higher than those of group III (p<0.01), which in turn did not differ from group II. The levels of total antioxidant capacity of the animals of group I was lower than those of groups II and III (p<0.01). ConclusionThese data confirmed the effective participation of oxidative stress in this model of renal ischemia-reperfusion syndrome in rats and showed that the use of antioxidant supplementation can protect the animals from oxidative changes.
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spelling Prevention of oxidative stress in renal ischemia-reperfusion syndrome in rats with nutritional antioxidant supplementationPrevenção do estresse oxidativo na síndrome de isquemia e reperfusão renal em ratos com suplementação nutricional com antioxidantesAntioxidantsOxidative stressFree radicalsReperfusion injuryVitaminsAntioxidantesEstresse oxidativoRadicais livresSíndrome de esquemiaTransplante de rinsTraumatismo por reperfusãoVitaminasObjectiveThe objective of this study was to verify the potential protective effect of antioxidant vitamin supplementation in a model of renal ischemic-reperfusion injury in rats. MethodsTwenty-nine Wistar rats were divided into three groups: groups I and II (n=10 each), were submitted to 60 minutes of renal ischemia, followed by 10 minutes of reperfusion; additionally, animals of group II were treated for twelve days with antioxidant vitamins (vitamin C 11.43mg/kg and vitamin E 28.5 mg/kg) before being submitted to ischemia; In group III (n=9), the animals were treated like the other groups but not submitted to ischemic-reperfusion injury and not given antioxidant supplements. Subsequently, blood samples and the kidneys were collected for assessment of malondialdehyde, uric acid and total antioxidant capacity. ResultsThe malondialdehyde and uric acid of group I was significantly higher than those of group III (p<0.01), which in turn did not differ from group II. The levels of total antioxidant capacity of the animals of group I was lower than those of groups II and III (p<0.01). ConclusionThese data confirmed the effective participation of oxidative stress in this model of renal ischemia-reperfusion syndrome in rats and showed that the use of antioxidant supplementation can protect the animals from oxidative changes.ObjetivoVerificar o potencial efeito protetor de suplementação com vitaminas antioxidantes em um modelo de síndrome de isquemia-reperfusão renal em ratos. MétodosForam utilizados 29 ratos Wistar, divididos em três grupos: Grupo I e II (n=10 cada), submetidos a indução do estresse oxidativo pela aplicação de 60 minutos de isquemia renal, seguidos de 10 minutos de reperfusão; adicionalmente, os animais do Grupo II foram pré-tratados por doze dias com vitaminas antioxidantes (vitamina C 11,43mg/kg e vitamina E 28,57mg/kg) antes da submissão à isquemia; Grupo III (n=9), correspondendo aos animais Sham, que foram manipulados de forma equivalente aos outros grupos, porém sem indução do estresse oxidativo e sem suplementação antioxidante. Findo isso, as amostras de sangue e os rins foram colhidos para avaliação dos níveis do malondialdeído, do ácido úrico e da capacidade antioxidante total. ResultadosPara o malondialdeído e ácido úrico do Grupo I foi observado um aumento estatisticamente significante (p<0,01) em relação ao Grupo III, o qual não apresentou diferença em relação ao Grupo II. Para os níveis de capacidade antioxidante total, foi encontrada uma diminuição nos animais do Grupo I em relação aos Grupos II e III (p<0,01). ConclusãoEsses dados confirmam não apenas a efetiva participação do estresse oxidativo neste modelo de síndrome de isquemia e reperfusão renal em ratos, como também que o uso de vitaminas antioxidantes, associadas à dieta, pode proteger os animais das alterações oxidativas.Núcleo de Editoração – PUC-Campinas2023-08-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/9358Brazilian Journal of Nutrition; Vol. 23 No. 2 (2010): Revista de NutriçãoRevista de Nutrição; Vol. 23 Núm. 2 (2010): Revista de NutriçãoRevista de Nutrição; v. 23 n. 2 (2010): Revista de Nutrição1678-9865reponame:Revista de Nutriçãoinstname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)instacron:PUC_CAMPporhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/9358/6732Copyright (c) 2023 Sandro PERCÁRIOhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessPERCÁRIO, Sandro 2023-08-25T18:34:52Zoai:ojs.periodicos.puc-campinas.edu.br:article/9358Revistahttp://www.scielo.br/rnPRIhttps://periodicos.puc-campinas.edu.br/nutricao/oai||sbi.submissionrn@puc-campinas.edu.br1678-98651415-5273opendoar:2023-08-25T18:34:52Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)false
dc.title.none.fl_str_mv Prevention of oxidative stress in renal ischemia-reperfusion syndrome in rats with nutritional antioxidant supplementation
Prevenção do estresse oxidativo na síndrome de isquemia e reperfusão renal em ratos com suplementação nutricional com antioxidantes
title Prevention of oxidative stress in renal ischemia-reperfusion syndrome in rats with nutritional antioxidant supplementation
spellingShingle Prevention of oxidative stress in renal ischemia-reperfusion syndrome in rats with nutritional antioxidant supplementation
PERCÁRIO, Sandro
Antioxidants
Oxidative stress
Free radicals
Reperfusion injury
Vitamins
Antioxidantes
Estresse oxidativo
Radicais livres
Síndrome de esquemia
Transplante de rins
Traumatismo por reperfusão
Vitaminas
title_short Prevention of oxidative stress in renal ischemia-reperfusion syndrome in rats with nutritional antioxidant supplementation
title_full Prevention of oxidative stress in renal ischemia-reperfusion syndrome in rats with nutritional antioxidant supplementation
title_fullStr Prevention of oxidative stress in renal ischemia-reperfusion syndrome in rats with nutritional antioxidant supplementation
title_full_unstemmed Prevention of oxidative stress in renal ischemia-reperfusion syndrome in rats with nutritional antioxidant supplementation
title_sort Prevention of oxidative stress in renal ischemia-reperfusion syndrome in rats with nutritional antioxidant supplementation
author PERCÁRIO, Sandro
author_facet PERCÁRIO, Sandro
author_role author
dc.contributor.author.fl_str_mv PERCÁRIO, Sandro
dc.subject.por.fl_str_mv Antioxidants
Oxidative stress
Free radicals
Reperfusion injury
Vitamins
Antioxidantes
Estresse oxidativo
Radicais livres
Síndrome de esquemia
Transplante de rins
Traumatismo por reperfusão
Vitaminas
topic Antioxidants
Oxidative stress
Free radicals
Reperfusion injury
Vitamins
Antioxidantes
Estresse oxidativo
Radicais livres
Síndrome de esquemia
Transplante de rins
Traumatismo por reperfusão
Vitaminas
description ObjectiveThe objective of this study was to verify the potential protective effect of antioxidant vitamin supplementation in a model of renal ischemic-reperfusion injury in rats. MethodsTwenty-nine Wistar rats were divided into three groups: groups I and II (n=10 each), were submitted to 60 minutes of renal ischemia, followed by 10 minutes of reperfusion; additionally, animals of group II were treated for twelve days with antioxidant vitamins (vitamin C 11.43mg/kg and vitamin E 28.5 mg/kg) before being submitted to ischemia; In group III (n=9), the animals were treated like the other groups but not submitted to ischemic-reperfusion injury and not given antioxidant supplements. Subsequently, blood samples and the kidneys were collected for assessment of malondialdehyde, uric acid and total antioxidant capacity. ResultsThe malondialdehyde and uric acid of group I was significantly higher than those of group III (p<0.01), which in turn did not differ from group II. The levels of total antioxidant capacity of the animals of group I was lower than those of groups II and III (p<0.01). ConclusionThese data confirmed the effective participation of oxidative stress in this model of renal ischemia-reperfusion syndrome in rats and showed that the use of antioxidant supplementation can protect the animals from oxidative changes.
publishDate 2023
dc.date.none.fl_str_mv 2023-08-25
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.puc-campinas.edu.br/nutricao/article/view/9358
url https://periodicos.puc-campinas.edu.br/nutricao/article/view/9358
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.puc-campinas.edu.br/nutricao/article/view/9358/6732
dc.rights.driver.fl_str_mv Copyright (c) 2023 Sandro PERCÁRIO
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Sandro PERCÁRIO
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Núcleo de Editoração – PUC-Campinas
publisher.none.fl_str_mv Núcleo de Editoração – PUC-Campinas
dc.source.none.fl_str_mv Brazilian Journal of Nutrition; Vol. 23 No. 2 (2010): Revista de Nutrição
Revista de Nutrição; Vol. 23 Núm. 2 (2010): Revista de Nutrição
Revista de Nutrição; v. 23 n. 2 (2010): Revista de Nutrição
1678-9865
reponame:Revista de Nutrição
instname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)
instacron:PUC_CAMP
instname_str Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)
instacron_str PUC_CAMP
institution PUC_CAMP
reponame_str Revista de Nutrição
collection Revista de Nutrição
repository.name.fl_str_mv Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)
repository.mail.fl_str_mv ||sbi.submissionrn@puc-campinas.edu.br
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