Influence of yeast (Saccharomyces cerevisiae) cell wall fractions on some nutritional parameters of growing rats

Detalhes bibliográficos
Autor(a) principal: Goulart CHAUD, Saula
Data de Publicação: 2023
Outros Autores: SGARBIERI, Valdemiro Carlos, VICENTE, Eduardo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista de Nutrição
Texto Completo: https://periodicos.puc-campinas.edu.br/nutricao/article/view/9565
Resumo: ObjectiveThe objective of the present work was to assess the nutritional impact of Saccharomyces cerevisiae cell wall fractions on some nutritional parameters in growing Wistar rats. MethodsYeast (Saccharomyces cerevisiae) biomass collected without undergoing thermolysis came from the mill São José, Zillo Lorenzetti (Macatuba, SP) in a suspension of approximately 20% p/v of cells. Fractionation of the cell wall material was done by differential extraction, centrifugation, and drying in “spray dryer”. The importance of the yeast cell components as dietary fibers was assessed in recently weaned Wistar rats by measuring weight gain, diet consumption (28 days), diet efficiency ratio, apparent protein digestibility, total amount of feces and lipids and cholesterol excreted in feces. ResultsRats which were submitted to diets containing glycan plus mannan gained less weight when compared with the other diets. The mannan-containing diet yielded the highest weight gain, followed by the standard AIN diet (S-AIN) and the insoluble glycan diet. Regarding diet efficiency ratio, the diet containing glycan plus mannan produced the lowest values throughout the 28 days. The highest apparent protein digestibility was obtained for the modified standard diet, for the standard AIN diet, as well as for the 10% mannan-containing diet (M). Total lipids and cholesterol excreted in the feces varied substantially among the diets. The dietcontaining 10% mannan was the one that promoted the greatest excretion of cholesterol. ConclusionAt the end of 28 days, the rats submitted to the glycan plus mannan-containing diets consumed less food and gained less body weight than those submitted to the other diets. Apparent digestibility of all diets was high, 98.6% on average. The amounts of total lipids and cholesterol excreted in the feces varied considerably; however, the mannan-containing diet promoted proportionally more cholesterol excretion than the other diets.
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spelling Influence of yeast (Saccharomyces cerevisiae) cell wall fractions on some nutritional parameters of growing ratsInfluência de frações da parede celular de levedura (Saccharomyces cerevisiae) sobre alguns parâmetros nutricionais de ratos em crescimentoMannansCell wallPolysaccharidesSaccharomyces cerevisiaeMananasParede celularPolissacarídeosSaccharomyces cerevisiaeObjectiveThe objective of the present work was to assess the nutritional impact of Saccharomyces cerevisiae cell wall fractions on some nutritional parameters in growing Wistar rats. MethodsYeast (Saccharomyces cerevisiae) biomass collected without undergoing thermolysis came from the mill São José, Zillo Lorenzetti (Macatuba, SP) in a suspension of approximately 20% p/v of cells. Fractionation of the cell wall material was done by differential extraction, centrifugation, and drying in “spray dryer”. The importance of the yeast cell components as dietary fibers was assessed in recently weaned Wistar rats by measuring weight gain, diet consumption (28 days), diet efficiency ratio, apparent protein digestibility, total amount of feces and lipids and cholesterol excreted in feces. ResultsRats which were submitted to diets containing glycan plus mannan gained less weight when compared with the other diets. The mannan-containing diet yielded the highest weight gain, followed by the standard AIN diet (S-AIN) and the insoluble glycan diet. Regarding diet efficiency ratio, the diet containing glycan plus mannan produced the lowest values throughout the 28 days. The highest apparent protein digestibility was obtained for the modified standard diet, for the standard AIN diet, as well as for the 10% mannan-containing diet (M). Total lipids and cholesterol excreted in the feces varied substantially among the diets. The dietcontaining 10% mannan was the one that promoted the greatest excretion of cholesterol. ConclusionAt the end of 28 days, the rats submitted to the glycan plus mannan-containing diets consumed less food and gained less body weight than those submitted to the other diets. Apparent digestibility of all diets was high, 98.6% on average. The amounts of total lipids and cholesterol excreted in the feces varied considerably; however, the mannan-containing diet promoted proportionally more cholesterol excretion than the other diets.ObjetivoO objetivo deste trabalho foi avaliar a influência das frações de parede celular de levedura (Saccharomyces cerevisiae) sobre alguns parâmetros nutricionais de ratos Wistar em crescimento. MétodosA biomassa de levedura (Saccharomyces cerevisiae), coletada sem sofrer o processo de termólise, foi recebida da usina São José, Zillo Lorenzetti (Macatuba, SP), em suspensão de, aproximadamente, (20% p/v) de células. O fracionamento da parede celular da levedura foi realizado por extração diferencial, centrifugação e secagem em spray dryer. A importância como fibra da dieta foi determinada em ratos da linhagem Wistar, recém desmamados, por meio das seguintes avaliações: ganho de peso corporal, consumo de dieta (28 dias), quociente de eficiência da dieta, digestibilidade aparente da proteína, quantidade total de fezes, lipídeos e colesterol excretados nas fezes. ResultadosOs animais que receberam a dieta contendo a fração glicana mais manana ganharam menos peso em relação aos demais tratamentos. A dieta com a fração manana foi a que proporcionou maior ganho de peso, seguida pela dieta padrão (AIN-P) e a dieta com 10% de glicana insolúvel. Quanto ao quociente de eficiência da dieta, observou-se, ao longo dos 28 dias, que a dieta com a fração glicana mais manana foi a que apresentou os menores valores. As maiores porcentagens de digestibilidade aparente da proteína foram observadas nas dietas: padrão modificada (AIN-M), padrão (AIN-P) e (M) com 10% da fração manana. As quantidades de lipídeos totais e colesterol excretados nas fezes variaram bastante entre as dietas, sendo que a dieta formulada com 10% de fração manana foi a que promoveu maior excreção do colesterol. ConclusãoAo final de 28 dias, os animais que receberam a dieta contendo 10,0% da fração glicana mais manana apresentaram o menor consumo de dieta e ganharam menos peso em relação às demais dietas. A digestibilidade aparente de todas as dietas foi elevada, em média 98,6%, contudo, as quantidades de lipídeos totais e colesterol excretados nas fezes variaram consideravelmente, sendo que a dieta contendo manana excretou, proporcionalmente, maior quantidade de colesterol.Núcleo de Editoração – PUC-Campinas2023-09-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/9565Brazilian Journal of Nutrition; Vol. 21 No. 2 (2008): Revista de NutriçãoRevista de Nutrição; Vol. 21 Núm. 2 (2008): Revista de NutriçãoRevista de Nutrição; v. 21 n. 2 (2008): Revista de Nutrição1678-9865reponame:Revista de Nutriçãoinstname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)instacron:PUC_CAMPporhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/9565/6925Copyright (c) 2023 Saula Goulart CHAUD, Valdemiro Carlos SGARBIERI, Eduardo VICENTEhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGoulart CHAUD, Saula SGARBIERI, Valdemiro Carlos VICENTE, Eduardo 2023-09-04T12:59:49Zoai:ojs.periodicos.puc-campinas.edu.br:article/9565Revistahttp://www.scielo.br/rnPRIhttps://periodicos.puc-campinas.edu.br/nutricao/oai||sbi.submissionrn@puc-campinas.edu.br1678-98651415-5273opendoar:2023-09-04T12:59:49Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)false
dc.title.none.fl_str_mv Influence of yeast (Saccharomyces cerevisiae) cell wall fractions on some nutritional parameters of growing rats
Influência de frações da parede celular de levedura (Saccharomyces cerevisiae) sobre alguns parâmetros nutricionais de ratos em crescimento
title Influence of yeast (Saccharomyces cerevisiae) cell wall fractions on some nutritional parameters of growing rats
spellingShingle Influence of yeast (Saccharomyces cerevisiae) cell wall fractions on some nutritional parameters of growing rats
Goulart CHAUD, Saula
Mannans
Cell wall
Polysaccharides
Saccharomyces cerevisiae
Mananas
Parede celular
Polissacarídeos
Saccharomyces cerevisiae
title_short Influence of yeast (Saccharomyces cerevisiae) cell wall fractions on some nutritional parameters of growing rats
title_full Influence of yeast (Saccharomyces cerevisiae) cell wall fractions on some nutritional parameters of growing rats
title_fullStr Influence of yeast (Saccharomyces cerevisiae) cell wall fractions on some nutritional parameters of growing rats
title_full_unstemmed Influence of yeast (Saccharomyces cerevisiae) cell wall fractions on some nutritional parameters of growing rats
title_sort Influence of yeast (Saccharomyces cerevisiae) cell wall fractions on some nutritional parameters of growing rats
author Goulart CHAUD, Saula
author_facet Goulart CHAUD, Saula
SGARBIERI, Valdemiro Carlos
VICENTE, Eduardo
author_role author
author2 SGARBIERI, Valdemiro Carlos
VICENTE, Eduardo
author2_role author
author
dc.contributor.author.fl_str_mv Goulart CHAUD, Saula
SGARBIERI, Valdemiro Carlos
VICENTE, Eduardo
dc.subject.por.fl_str_mv Mannans
Cell wall
Polysaccharides
Saccharomyces cerevisiae
Mananas
Parede celular
Polissacarídeos
Saccharomyces cerevisiae
topic Mannans
Cell wall
Polysaccharides
Saccharomyces cerevisiae
Mananas
Parede celular
Polissacarídeos
Saccharomyces cerevisiae
description ObjectiveThe objective of the present work was to assess the nutritional impact of Saccharomyces cerevisiae cell wall fractions on some nutritional parameters in growing Wistar rats. MethodsYeast (Saccharomyces cerevisiae) biomass collected without undergoing thermolysis came from the mill São José, Zillo Lorenzetti (Macatuba, SP) in a suspension of approximately 20% p/v of cells. Fractionation of the cell wall material was done by differential extraction, centrifugation, and drying in “spray dryer”. The importance of the yeast cell components as dietary fibers was assessed in recently weaned Wistar rats by measuring weight gain, diet consumption (28 days), diet efficiency ratio, apparent protein digestibility, total amount of feces and lipids and cholesterol excreted in feces. ResultsRats which were submitted to diets containing glycan plus mannan gained less weight when compared with the other diets. The mannan-containing diet yielded the highest weight gain, followed by the standard AIN diet (S-AIN) and the insoluble glycan diet. Regarding diet efficiency ratio, the diet containing glycan plus mannan produced the lowest values throughout the 28 days. The highest apparent protein digestibility was obtained for the modified standard diet, for the standard AIN diet, as well as for the 10% mannan-containing diet (M). Total lipids and cholesterol excreted in the feces varied substantially among the diets. The dietcontaining 10% mannan was the one that promoted the greatest excretion of cholesterol. ConclusionAt the end of 28 days, the rats submitted to the glycan plus mannan-containing diets consumed less food and gained less body weight than those submitted to the other diets. Apparent digestibility of all diets was high, 98.6% on average. The amounts of total lipids and cholesterol excreted in the feces varied considerably; however, the mannan-containing diet promoted proportionally more cholesterol excretion than the other diets.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.puc-campinas.edu.br/nutricao/article/view/9565
url https://periodicos.puc-campinas.edu.br/nutricao/article/view/9565
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.puc-campinas.edu.br/nutricao/article/view/9565/6925
dc.rights.driver.fl_str_mv Copyright (c) 2023 Saula Goulart CHAUD, Valdemiro Carlos SGARBIERI, Eduardo VICENTE
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Saula Goulart CHAUD, Valdemiro Carlos SGARBIERI, Eduardo VICENTE
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Núcleo de Editoração – PUC-Campinas
publisher.none.fl_str_mv Núcleo de Editoração – PUC-Campinas
dc.source.none.fl_str_mv Brazilian Journal of Nutrition; Vol. 21 No. 2 (2008): Revista de Nutrição
Revista de Nutrição; Vol. 21 Núm. 2 (2008): Revista de Nutrição
Revista de Nutrição; v. 21 n. 2 (2008): Revista de Nutrição
1678-9865
reponame:Revista de Nutrição
instname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)
instacron:PUC_CAMP
instname_str Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)
instacron_str PUC_CAMP
institution PUC_CAMP
reponame_str Revista de Nutrição
collection Revista de Nutrição
repository.name.fl_str_mv Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)
repository.mail.fl_str_mv ||sbi.submissionrn@puc-campinas.edu.br
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