Effects of acute restraint stress, chronic swim stress and glutamine administration on the release of superoxide from alveolar macrophages of rats

• Autor(a) principal: do NASCIMENTO, Elizabeth
• Data de Publicação: 2023
• Outros Autores: Góis LEANDRO, Carol Virgínia, Fidalgo AMORIM, Marco Antônio, PALMEIRA, América, Cavalcante FERRO, Taisy, Machado Barbosa de CASTRO, Célia Maria, Manhães de CASTRO, Raul
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista de Nutrição
• Texto Completo: https://periodicos.puc-campinas.edu.br/nutricao/article/view/9694
• topic: macrophages; alveolar; superoxide anion; stress; swimming; glutamine; macrófagos alveolares; liberação de ânion superóxido; estresse; natação; glutamina

ObjectiveTo assess the release of superoxide anion from alveolar macrophages of rats submitted or not to acute restraint stress, forced swimming and glutamine supplementation. MethodsForty-two male Wistar rats aging roughly 62 days (standard deviation=3) were randomly divided into four groups: control, training, stress and glutamine. After the intervention, alveolar macrophages were collected and stimulated with phorbol myristate acetate to assess the release of superoxide anion. ResultsWhen compared with the first hour (control=26.2, standard deviation=4.2; training=28.7, standard deviation=5.1; stress=20.3 , standard deviation=4.4; glutamine=26.2, standard deviation=4.2), the release of superoxide increased (p<0.001) in all experimental groups in the second hour (control=38.4, standard deviation=4.9; training=40.7, standard deviation=6.1; stress=30.2, standard deviation=5.6; glutamine=39.2, standard deviation=5.2) of observation. Training and glutamine supplementation did not induce differences in the release of superoxide from alveolar macrophages when compared with the control group. Only the ratssubmitted to stress showed a reduction in the release of superoxide in both the first (20.3, standard deviation=4.4; p<0.05) and second hours (30.2, standard deviation=5.6; p<0.05) of observation. ConclusionThe results suggest that stress can be one of the factors associated with immunosuppression since reduced release of superoxide anion from macrophages can lead to reduced microbicidal capacity. On the other hand, the swimming protocol we used and the amount and route of glutamine supplementation did not change the release of superoxide from alveolar macrophages.