Neonatal malnutrition and normal microbiota of the oral cavity in rats
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista de Nutrição |
Texto Completo: | https://periodicos.puc-campinas.edu.br/nutricao/article/view/9717 |
Resumo: | ObjectiveTo evaluate the influence of neonatal malnutrition on the pattern and growth of aerobic bacteria of the normal bacterial flora of the oral cavity in adults Wistar rats. MethodsIn the present study, the material of the oral cavity was collected through swabs soaked in 40µL of sterile saline solution. After the collection, each swab was placed in a sterile tube containing 960µL of brain heart infusion. Later, the samples were homogenized. Then, from the 1.000µL, 1µL was collected with a gauged loop to be sowed in Petri dishes containing Agar-blood and Agar-Levine, for the isolation and identification of the Grampositive and Gram-negative bacteria respectively. The plates were placed into a bacteriological incubator, 37ºC, for 48 hours and the colony-forming units that grew were counted and their percentages were calculated. For bacterioscopy, slides were stained with the Gram method. ResultsFrom the 5th to the 21st day of life, body weight of the undernourished group (33.6g:42.8g, standard deviation=27.2g) was smaller (p<0.001) than that of the nourished group (52.5g:56.3g, standard deviation=24.0g). From the 60th to the 120th days of life the body weight of the undernourished group (7.4.x.106 colony-forming units, standard deviation=1,0x106 colony-forming units bacteria/mL) decreased when compared to that of the nourished group (306.2g:319.4g, standard deviation=219.5g), Mann-Whitney test, p<0,05. The microbiota was composed of a smaller number of bacteria in the nourished group (4.2 x106 colony-forming units, standard deviation=1.2x106 colony-forming units bacteria/mL) when compared with the undernourished group (7.4x106 colony-forming units, standard deviation=1.0x106 colony-forming units bacteria/mL), Student’s “t” test (p=0.026). From the bacteria isolated in cultures, a similar bacterial pattern was observed in both groups, with 70% of Gram-positive bacteria and 30% of Gram-negative bacteria. ConclusionNeonatal malnutrition was effective in decreasing body weight and increasing bacterial growth. Although the bacterial pattern was not altered, the increase in bacterial growth may induce an imbalance of the oral microbiota, being a threat to the host. |
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Neonatal malnutrition and normal microbiota of the oral cavity in ratsDesnutrição neonatal e microbiota normal da cavidade oral em ratosbacteria, aerobicmouthneonatal malnutritionrats Wistarbactérias aeróbiasbocadesnutrição neonatalratos WistarObjectiveTo evaluate the influence of neonatal malnutrition on the pattern and growth of aerobic bacteria of the normal bacterial flora of the oral cavity in adults Wistar rats. MethodsIn the present study, the material of the oral cavity was collected through swabs soaked in 40µL of sterile saline solution. After the collection, each swab was placed in a sterile tube containing 960µL of brain heart infusion. Later, the samples were homogenized. Then, from the 1.000µL, 1µL was collected with a gauged loop to be sowed in Petri dishes containing Agar-blood and Agar-Levine, for the isolation and identification of the Grampositive and Gram-negative bacteria respectively. The plates were placed into a bacteriological incubator, 37ºC, for 48 hours and the colony-forming units that grew were counted and their percentages were calculated. For bacterioscopy, slides were stained with the Gram method. ResultsFrom the 5th to the 21st day of life, body weight of the undernourished group (33.6g:42.8g, standard deviation=27.2g) was smaller (p<0.001) than that of the nourished group (52.5g:56.3g, standard deviation=24.0g). From the 60th to the 120th days of life the body weight of the undernourished group (7.4.x.106 colony-forming units, standard deviation=1,0x106 colony-forming units bacteria/mL) decreased when compared to that of the nourished group (306.2g:319.4g, standard deviation=219.5g), Mann-Whitney test, p<0,05. The microbiota was composed of a smaller number of bacteria in the nourished group (4.2 x106 colony-forming units, standard deviation=1.2x106 colony-forming units bacteria/mL) when compared with the undernourished group (7.4x106 colony-forming units, standard deviation=1.0x106 colony-forming units bacteria/mL), Student’s “t” test (p=0.026). From the bacteria isolated in cultures, a similar bacterial pattern was observed in both groups, with 70% of Gram-positive bacteria and 30% of Gram-negative bacteria. ConclusionNeonatal malnutrition was effective in decreasing body weight and increasing bacterial growth. Although the bacterial pattern was not altered, the increase in bacterial growth may induce an imbalance of the oral microbiota, being a threat to the host. ObjetivoAvaliar a influência da desnutrição neonatal sobre o padrão e o crescimento de bactérias aeróbias, da microbiota normal da cavidade oral, em ratos Wistar adultos. MétodosO material da cavidade oral foi coletado através de swabs embebidos em 40µL de solução salina estéril e colocados em tubos estéreis contendo 960µL de brain heart infusion. Posteriormente, fez-se homogeneização de cada uma amostra. Então, destes 1.000µL, retirou-se 1µL e este foi semeado em placas de Petri contendo Agar-sangue e Levine para isolamento e identificação de bactérias Gram+ e Gram-, respectivamente. Essas placas foram incubadas em estufa bacteriológica a 37oC, 48 horas, e as unidades formadoras de colônias que cresceram foram contadas e seus percentuais calculados. Para a bacterioscopia foram confeccionadas lâminas coradas pelo método de Gram. ResultadosDo 5o ao 21o dia de vida os pesos corporais do grupo desnutrido (33,6g:42,8g, desvio-padrão=27,2g) foram menores (p<0,001) que os do grupo nutrido (52,5g:56,3g, desvio-padrão=24,0g). Dos 60 aos 120 dias de vida os pesos foram reduzidos no grupo desnutrido (248,2g:290,2g, desvio-padrão=220,2g), quando comparados aos do grupo nutrido (306,2g:319,4g, desvio-padrão=219,5g) teste Mann Whitney, p<0,05. A microbiota foi composta de menor número de bactérias no grupo nutrido (4,2x106 unidades formadoras de colônias, desvio-padrão=1,2x106 unidades formadoras de colônias de bactérias/mL), comparado ao grupo desnutrido (7,4x106 unidades formadoras de colônias, desvio-padrão=1,0x106 unidades formadoras de colônias de bactérias/mL) teste “t”, (p=0,026). Das bactérias isoladas em culturas, observou-se um padrão bacteriano semelhante nos dois grupos, com 70% de bactérias gram-positivas e 30% de bactérias gram-negativas. ConclusãoA desnutrição neonatal foi efetiva em reduzir o peso corporal e aumentar o crescimento bacteriano. Embora o padrão bacteriano não tenha sido alterado, o aumento do crescimento bacteriano pode induzir a um desequilíbrio na microbiota oral desfavorecendo assim, o hospedeiro. Núcleo de Editoração – PUC-Campinas2023-09-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/9717Brazilian Journal of Nutrition; Vol. 20 No. 6 (2007): Revista de NutriçãoRevista de Nutrição; Vol. 20 Núm. 6 (2007): Revista de NutriçãoRevista de Nutrição; v. 20 n. 6 (2007): Revista de Nutrição1678-9865reponame:Revista de Nutriçãoinstname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)instacron:PUC_CAMPporhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/9717/7063Copyright (c) 2023 Solange Maria Magalhães da Silva PORTO, Marcelo Tavares VIANA, Karla Melo Ferreira da SILVA, Maria de Fátima Alves DINIZ, Célia Maria Machado Barbosa de CASTROhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess Magalhães da Silva PORTO, Solange MariaTavares VIANA, MarceloFerreira da SILVA, Karla Melo Alves DINIZ, Maria de FátimaMachado Barbosa de CASTRO, Célia Maria 2023-10-05T18:43:47Zoai:ojs.periodicos.puc-campinas.edu.br:article/9717Revistahttp://www.scielo.br/rnPRIhttps://periodicos.puc-campinas.edu.br/nutricao/oai||sbi.submissionrn@puc-campinas.edu.br1678-98651415-5273opendoar:2023-10-05T18:43:47Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)false |
dc.title.none.fl_str_mv |
Neonatal malnutrition and normal microbiota of the oral cavity in rats Desnutrição neonatal e microbiota normal da cavidade oral em ratos |
title |
Neonatal malnutrition and normal microbiota of the oral cavity in rats |
spellingShingle |
Neonatal malnutrition and normal microbiota of the oral cavity in rats Magalhães da Silva PORTO, Solange Maria bacteria, aerobic mouth neonatal malnutrition rats Wistar bactérias aeróbias boca desnutrição neonatal ratos Wistar |
title_short |
Neonatal malnutrition and normal microbiota of the oral cavity in rats |
title_full |
Neonatal malnutrition and normal microbiota of the oral cavity in rats |
title_fullStr |
Neonatal malnutrition and normal microbiota of the oral cavity in rats |
title_full_unstemmed |
Neonatal malnutrition and normal microbiota of the oral cavity in rats |
title_sort |
Neonatal malnutrition and normal microbiota of the oral cavity in rats |
author |
Magalhães da Silva PORTO, Solange Maria |
author_facet |
Magalhães da Silva PORTO, Solange Maria Tavares VIANA, Marcelo Ferreira da SILVA, Karla Melo Alves DINIZ, Maria de Fátima Machado Barbosa de CASTRO, Célia Maria |
author_role |
author |
author2 |
Tavares VIANA, Marcelo Ferreira da SILVA, Karla Melo Alves DINIZ, Maria de Fátima Machado Barbosa de CASTRO, Célia Maria |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Magalhães da Silva PORTO, Solange Maria Tavares VIANA, Marcelo Ferreira da SILVA, Karla Melo Alves DINIZ, Maria de Fátima Machado Barbosa de CASTRO, Célia Maria |
dc.subject.por.fl_str_mv |
bacteria, aerobic mouth neonatal malnutrition rats Wistar bactérias aeróbias boca desnutrição neonatal ratos Wistar |
topic |
bacteria, aerobic mouth neonatal malnutrition rats Wistar bactérias aeróbias boca desnutrição neonatal ratos Wistar |
description |
ObjectiveTo evaluate the influence of neonatal malnutrition on the pattern and growth of aerobic bacteria of the normal bacterial flora of the oral cavity in adults Wistar rats. MethodsIn the present study, the material of the oral cavity was collected through swabs soaked in 40µL of sterile saline solution. After the collection, each swab was placed in a sterile tube containing 960µL of brain heart infusion. Later, the samples were homogenized. Then, from the 1.000µL, 1µL was collected with a gauged loop to be sowed in Petri dishes containing Agar-blood and Agar-Levine, for the isolation and identification of the Grampositive and Gram-negative bacteria respectively. The plates were placed into a bacteriological incubator, 37ºC, for 48 hours and the colony-forming units that grew were counted and their percentages were calculated. For bacterioscopy, slides were stained with the Gram method. ResultsFrom the 5th to the 21st day of life, body weight of the undernourished group (33.6g:42.8g, standard deviation=27.2g) was smaller (p<0.001) than that of the nourished group (52.5g:56.3g, standard deviation=24.0g). From the 60th to the 120th days of life the body weight of the undernourished group (7.4.x.106 colony-forming units, standard deviation=1,0x106 colony-forming units bacteria/mL) decreased when compared to that of the nourished group (306.2g:319.4g, standard deviation=219.5g), Mann-Whitney test, p<0,05. The microbiota was composed of a smaller number of bacteria in the nourished group (4.2 x106 colony-forming units, standard deviation=1.2x106 colony-forming units bacteria/mL) when compared with the undernourished group (7.4x106 colony-forming units, standard deviation=1.0x106 colony-forming units bacteria/mL), Student’s “t” test (p=0.026). From the bacteria isolated in cultures, a similar bacterial pattern was observed in both groups, with 70% of Gram-positive bacteria and 30% of Gram-negative bacteria. ConclusionNeonatal malnutrition was effective in decreasing body weight and increasing bacterial growth. Although the bacterial pattern was not altered, the increase in bacterial growth may induce an imbalance of the oral microbiota, being a threat to the host. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-09-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/9717 |
url |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/9717 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/9717/7063 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Núcleo de Editoração – PUC-Campinas |
publisher.none.fl_str_mv |
Núcleo de Editoração – PUC-Campinas |
dc.source.none.fl_str_mv |
Brazilian Journal of Nutrition; Vol. 20 No. 6 (2007): Revista de Nutrição Revista de Nutrição; Vol. 20 Núm. 6 (2007): Revista de Nutrição Revista de Nutrição; v. 20 n. 6 (2007): Revista de Nutrição 1678-9865 reponame:Revista de Nutrição instname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) instacron:PUC_CAMP |
instname_str |
Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) |
instacron_str |
PUC_CAMP |
institution |
PUC_CAMP |
reponame_str |
Revista de Nutrição |
collection |
Revista de Nutrição |
repository.name.fl_str_mv |
Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) |
repository.mail.fl_str_mv |
||sbi.submissionrn@puc-campinas.edu.br |
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1799126075071004672 |