Acute hypothalamic administration of L-arginine increases feed intake in rats
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista de Nutrição |
Texto Completo: | https://periodicos.puc-campinas.edu.br/nutricao/article/view/8192 |
Resumo: | ObjectiveThis study investigated the chronic (oral) and acute (hypothalamic infusion) effects of L-arginine supplementation on feed intake, body composition, and behavioral changes in rats. MethodsTwenty rats were divided into two groups treated orally for 60 days; one group received L-arginine (1 g/kg body weight) and one group received saline (1 mL/NaCl 0.9%). Daily consumption of water and food were evaluated, and weight monitored. After the oral treatment, the rats underwent stereotactic biopsy and a group was injected with 2 µL of L-arginine (0.5 mM) and another received an injection of saline (0.9% NaCl), in the hypothalamic route, through micro infusion. Immediately after micro infusion, the animal behavior was evaluated through tests in the open field. Food and water consumption were evaluated at 12 and 24 hours after themicro infusion. Daily water consumption and weight gain evolution were evaluated. At the end of treatments, rats were euthanized and blood was collected for glucose, glycerol, and cholesterol evaluation, and histological analysis of vital organs. ResultsOral supplementation with L-arginine increased water intake (11%, p<0.05) and promoted weight gain (3%, p<0.05). However, hypothalamic infusion promoted a significant increase in chow intake (30%, p<0.05) after 24 hours of L-arginine administration. ConclusionChronic oral treatment with L-arginine was not effective on appetite modulation; however, an effect was observed when L-arginine was administered directly into the hypothalamus, suggesting a central regulation on appetite through nNOS sensitization. Chronic use of L-arginine did not cause substantial changes in anthropometric, biochemical, behavioral, or histological variables. |
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Acute hypothalamic administration of L-arginine increases feed intake in ratsAdministração hipotalâmica aguda de L-arginina aumenta a ingestão alimentar em ratosCentral nervous systemEatingFeeding behaviorSistema nervoso centralIngestão de alimentosComportamento alimentarObjectiveThis study investigated the chronic (oral) and acute (hypothalamic infusion) effects of L-arginine supplementation on feed intake, body composition, and behavioral changes in rats. MethodsTwenty rats were divided into two groups treated orally for 60 days; one group received L-arginine (1 g/kg body weight) and one group received saline (1 mL/NaCl 0.9%). Daily consumption of water and food were evaluated, and weight monitored. After the oral treatment, the rats underwent stereotactic biopsy and a group was injected with 2 µL of L-arginine (0.5 mM) and another received an injection of saline (0.9% NaCl), in the hypothalamic route, through micro infusion. Immediately after micro infusion, the animal behavior was evaluated through tests in the open field. Food and water consumption were evaluated at 12 and 24 hours after themicro infusion. Daily water consumption and weight gain evolution were evaluated. At the end of treatments, rats were euthanized and blood was collected for glucose, glycerol, and cholesterol evaluation, and histological analysis of vital organs. ResultsOral supplementation with L-arginine increased water intake (11%, p<0.05) and promoted weight gain (3%, p<0.05). However, hypothalamic infusion promoted a significant increase in chow intake (30%, p<0.05) after 24 hours of L-arginine administration. ConclusionChronic oral treatment with L-arginine was not effective on appetite modulation; however, an effect was observed when L-arginine was administered directly into the hypothalamus, suggesting a central regulation on appetite through nNOS sensitization. Chronic use of L-arginine did not cause substantial changes in anthropometric, biochemical, behavioral, or histological variables.ObjetivoEste estudo investigou os efeitos da suplementação crônica (oral) e aguda (infusão no hipotálamo) com L-arginina sobre a saciedade, composição corporal e mudanças comportamentais em ratos. MétodosVinte ratos foram divididos em dois grupos e tratados por via oral durante 60 dias; um grupo recebeu uma dose de L-arginina (1 g/kg de peso corporal), outro grupo recebeu uma dose de solução salina (1 mL/NaCl 0.9%). O consumo diário de água e comida e a evolução do ganho de peso foram avaliados. Após o tratamento por via oral, os ratos foram submetidos à estereotaxia: um grupo recebeu uma injeção com 2 µl de L-Arginina (0.5 mM) e outro recebeu uma injeção de solução salina (NaCl 0,9%), no hipotálamo, através de microinfusão. Imediatamente após a microinfusão, foi avaliado o comportamento dos animais através do teste em campo aberto. O consumo de água e de comida foi avaliado nas 12 e 24 horas seguintes à microinfusão. No final dos tratamentos, os ratos foram eutanasiados para coleta de amostras para dosagem de glicose, glicerol e colesterol, além da análise histológica dos órgãos. ResultadosA suplementação oral com L-arginina promoveu aumento do consumo de água (11%, p<0,05) e ganho de peso (3%, p<0,05). Além disso, a infusão hipotalâmica promoveu um aumento significativo do consumo alimentar (30%, p<0,05) após 24 horas da administração de L-arginina. ConclusãoO tratamento oral crônico com L-arginina não obteve efeitos na modulação do apetite. No entanto, ocorreu aumento da ingestão de alimento quando a L-arginina foi administrada diretamente no hipotálamo, sugerindo que exista uma estimulação do apetite através da sensibilização da nNOS nessa região. O uso crônico de L-arginina não causou mudanças substanciais nos dados antropométricos, bioquímicos, comportamentais ou histológicos.Núcleo de Editoração – PUC-Campinas2023-03-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/8192Brazilian Journal of Nutrition; Vol. 28 No. 1 (2015): Revista de NutriçãoRevista de Nutrição; Vol. 28 Núm. 1 (2015): Revista de NutriçãoRevista de Nutrição; v. 28 n. 1 (2015): Revista de Nutrição1678-9865reponame:Revista de Nutriçãoinstname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)instacron:PUC_CAMPporhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/8192/5714Copyright (c) 2023 Carlos Ricardo Maneck MALFATTI, Luiz Augusto da SILVA, Ricardo Aparecido PEREIRA, Renan Garcia MICHEL, André Luiz SNAK, Fabio Seidel dos SANTOShttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessManeck MALFATTI, Carlos Ricardo da SILVA, Luiz Augusto Aparecido PEREIRA, Ricardo Garcia MICHEL, RenanSNAK, André Luiz Seidel dos SANTOS, Fabio 2023-03-31T12:28:38Zoai:ojs.periodicos.puc-campinas.edu.br:article/8192Revistahttp://www.scielo.br/rnPRIhttps://periodicos.puc-campinas.edu.br/nutricao/oai||sbi.submissionrn@puc-campinas.edu.br1678-98651415-5273opendoar:2023-03-31T12:28:38Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)false |
dc.title.none.fl_str_mv |
Acute hypothalamic administration of L-arginine increases feed intake in rats Administração hipotalâmica aguda de L-arginina aumenta a ingestão alimentar em ratos |
title |
Acute hypothalamic administration of L-arginine increases feed intake in rats |
spellingShingle |
Acute hypothalamic administration of L-arginine increases feed intake in rats Maneck MALFATTI, Carlos Ricardo Central nervous system Eating Feeding behavior Sistema nervoso central Ingestão de alimentos Comportamento alimentar |
title_short |
Acute hypothalamic administration of L-arginine increases feed intake in rats |
title_full |
Acute hypothalamic administration of L-arginine increases feed intake in rats |
title_fullStr |
Acute hypothalamic administration of L-arginine increases feed intake in rats |
title_full_unstemmed |
Acute hypothalamic administration of L-arginine increases feed intake in rats |
title_sort |
Acute hypothalamic administration of L-arginine increases feed intake in rats |
author |
Maneck MALFATTI, Carlos Ricardo |
author_facet |
Maneck MALFATTI, Carlos Ricardo da SILVA, Luiz Augusto Aparecido PEREIRA, Ricardo Garcia MICHEL, Renan SNAK, André Luiz Seidel dos SANTOS, Fabio |
author_role |
author |
author2 |
da SILVA, Luiz Augusto Aparecido PEREIRA, Ricardo Garcia MICHEL, Renan SNAK, André Luiz Seidel dos SANTOS, Fabio |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Maneck MALFATTI, Carlos Ricardo da SILVA, Luiz Augusto Aparecido PEREIRA, Ricardo Garcia MICHEL, Renan SNAK, André Luiz Seidel dos SANTOS, Fabio |
dc.subject.por.fl_str_mv |
Central nervous system Eating Feeding behavior Sistema nervoso central Ingestão de alimentos Comportamento alimentar |
topic |
Central nervous system Eating Feeding behavior Sistema nervoso central Ingestão de alimentos Comportamento alimentar |
description |
ObjectiveThis study investigated the chronic (oral) and acute (hypothalamic infusion) effects of L-arginine supplementation on feed intake, body composition, and behavioral changes in rats. MethodsTwenty rats were divided into two groups treated orally for 60 days; one group received L-arginine (1 g/kg body weight) and one group received saline (1 mL/NaCl 0.9%). Daily consumption of water and food were evaluated, and weight monitored. After the oral treatment, the rats underwent stereotactic biopsy and a group was injected with 2 µL of L-arginine (0.5 mM) and another received an injection of saline (0.9% NaCl), in the hypothalamic route, through micro infusion. Immediately after micro infusion, the animal behavior was evaluated through tests in the open field. Food and water consumption were evaluated at 12 and 24 hours after themicro infusion. Daily water consumption and weight gain evolution were evaluated. At the end of treatments, rats were euthanized and blood was collected for glucose, glycerol, and cholesterol evaluation, and histological analysis of vital organs. ResultsOral supplementation with L-arginine increased water intake (11%, p<0.05) and promoted weight gain (3%, p<0.05). However, hypothalamic infusion promoted a significant increase in chow intake (30%, p<0.05) after 24 hours of L-arginine administration. ConclusionChronic oral treatment with L-arginine was not effective on appetite modulation; however, an effect was observed when L-arginine was administered directly into the hypothalamus, suggesting a central regulation on appetite through nNOS sensitization. Chronic use of L-arginine did not cause substantial changes in anthropometric, biochemical, behavioral, or histological variables. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-03-31 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/8192 |
url |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/8192 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/8192/5714 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Núcleo de Editoração – PUC-Campinas |
publisher.none.fl_str_mv |
Núcleo de Editoração – PUC-Campinas |
dc.source.none.fl_str_mv |
Brazilian Journal of Nutrition; Vol. 28 No. 1 (2015): Revista de Nutrição Revista de Nutrição; Vol. 28 Núm. 1 (2015): Revista de Nutrição Revista de Nutrição; v. 28 n. 1 (2015): Revista de Nutrição 1678-9865 reponame:Revista de Nutrição instname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) instacron:PUC_CAMP |
instname_str |
Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) |
instacron_str |
PUC_CAMP |
institution |
PUC_CAMP |
reponame_str |
Revista de Nutrição |
collection |
Revista de Nutrição |
repository.name.fl_str_mv |
Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) |
repository.mail.fl_str_mv |
||sbi.submissionrn@puc-campinas.edu.br |
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1799126069089927168 |