Effect of a commercial enteral diet with added antioxidants on total plasma thiol, protein carbonyl and malondialdehyde levels after a stroke

• Autor(a) principal: Simioni YASSIN, Lorene
• Data de Publicação: 2023
• Outros Autores: FUKUI, Chika, Dias PEREIRA, Pamela Cristiani, OLANDOSKI, Marcia, AVELES, Paulo Roberto, PINTO, Silvia Carolina, RESNAUER, Caroline, NAKAO, Lia Sumie, Ikeda MORIMOTO, Ivone
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista de Nutrição
• Texto Completo: https://periodicos.puc-campinas.edu.br/nutricao/article/view/9241
• topic: Cerebrovascular accident; Carbonyl stress; Lipid peroxidation; Supplementary feeding; Nutrition therapy; Acidente cerebrovascular; Estresse carbonílico; Peroxidação de lipídeos; Suplementação alimentar; Terapia nutricional

ObjectiveThe aim of this study was to assess the effect of a commercial enteral diet with added antioxidants on total plasma thiol, protein carbonyl and malondialdehyde levels of stroke survivors. MethodsFourteen patients from a general hospital who had been started on enteral nutrition 48 hours after a stroke were included in the study. The exclusion criteria were multiple organ dysfunction syndrome, liver failure and morbid obesity associated with diabetes Mellitus. The commercial diet was fed by continuous drip via infusion pump and contained mixed carotenoids, vitamins C and E, and the minerals selenium, zinc and copper. Blood samples were collected at baseline and after 5 days of enteral diet, but only from patients whose diet intake met their total energy expenditure. Total plasma thiol and protein carbonyl levels were determined by Ellman’s reagent and dinitrophenylhydrazine, respectively. Plasma malondialdehyde levels were measured by the assayof thiobarbituric acid reactive substances. ResultsThe mean age of the sample was M=70.3 years, (SD=14.1). All patients received more than 100% of the Dietary Reference Intakes for the abovementioned antioxidants but none exceeded the tolerable upper limit. Plasma thiol and malondialdehyde levels did not vary over time but protein carbonyl levels were significantly higher (p=0.034), especially in intubated patients (p=0.048). ConclusionBiomarker determinations showed that a commercial enteral diet with added antioxidants did not reduce oxidative stress.