Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista odonto ciência (Online) |
Texto Completo: | https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5043 |
Resumo: | Purpose: This study evaluated the quality of DNA obtained from stored human saliva and its applicability to human identification. Methods: The saliva samples of 20 subjects, collected in the form of saliva in natura and from mouth swabs and stored at -20ºC, were analyzed. After 7 days, the DNA was extracted from the 40 saliva samples and subjected to PCR and electrophoresis. After 180 days, the technique was repeated with the 20 swab samples. Results: The first-stage results indicated that DNA was successfully extracted in 97.5% of reactions, 95% of saliva in natura and 100% of swab saliva samples, with no statistically significant difference between the forms of saliva. In the second phase, the result was positive for all 20 analyzed samples (100%). Subsequently, in order to analyze the quality of the DNA obtained from human saliva, the SIX3-2 gene was tested on the 20 mouth swab samples, and the PCR products were digested using the MbO1 restriction enzyme to evaluate polymorphisms in the ADRA-2 gene, with positive results for most samples. Conclusion: It was concluded that the quantity and quality of DNA from saliva and the techniques employed are adequate for forensic analysis of DNA. |
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Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic DentistryAvaliação da qualidade do DNA extraído de saliva humana armazenada e sua aplicabilidade na identificação em Odontologia LegalHuman identificationForensic DentistryForensic MedicineDNAmolecular biologysalivaIdentificação HumanaPurpose: This study evaluated the quality of DNA obtained from stored human saliva and its applicability to human identification. Methods: The saliva samples of 20 subjects, collected in the form of saliva in natura and from mouth swabs and stored at -20ºC, were analyzed. After 7 days, the DNA was extracted from the 40 saliva samples and subjected to PCR and electrophoresis. After 180 days, the technique was repeated with the 20 swab samples. Results: The first-stage results indicated that DNA was successfully extracted in 97.5% of reactions, 95% of saliva in natura and 100% of swab saliva samples, with no statistically significant difference between the forms of saliva. In the second phase, the result was positive for all 20 analyzed samples (100%). Subsequently, in order to analyze the quality of the DNA obtained from human saliva, the SIX3-2 gene was tested on the 20 mouth swab samples, and the PCR products were digested using the MbO1 restriction enzyme to evaluate polymorphisms in the ADRA-2 gene, with positive results for most samples. Conclusion: It was concluded that the quantity and quality of DNA from saliva and the techniques employed are adequate for forensic analysis of DNA.Este trabalho objetivou avaliar a qualidade do DNA obtido de saliva humana armazenada e sua aplicabilidade da identificação de pessoas. Metodologia: Analisaram-se amostras salivares de n=20 sujeitos da pesquisa, coletadas nas formas de saliva in natura e de swab bucal, sendo armazenadas a 20ºC. Após 7 dias, o DNA foi extraído das 40 amostras de saliva e submetido à PCR e à eletroforese. Após 180 dias repetiu-se a técnica nas 20 amostras de swab. Resultados: Os resultados da primeira etapa indicaram que o DNA foi extraído com sucesso em 97,5% das reações, e, analisando-se separadamente, em 95% de saliva in natura e em 100% da saliva do swab, não havendo diferenças estatisticamente significantes entre as duas formas de saliva. Na segunda fase, o resultado foi positivo para as 20 amostras analisadas (100%). Posteriormente, para analisar a qualidade do DNA obtido da saliva humana, o gene SIX3-2 foi testado nas 20 amostras de swab bucal e foi feita a digestão do produto da PCR com a enzima de restrição MbO1 para avaliar polimorfismo do gene ADRA-2 obtendo-se resultados positivos para a maioria das amostras. Conclusão: Concluiu-se que a quantidade e a qualidade do DNA advindo de saliva e as técnicas empregadas estão adequadas à análise forense do DNA.EDIPUCRS - Editora Universitária da PUCRS2010-01-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articleapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5043Revista Odonto Ciência; Vol. 25 No. 1 (2010); 48 - 53Revista Odonto Ciência; v. 25 n. 1 (2010); 48 - 531980-65230102-9460reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5043/4861Carvalho, Suzana Papile MacielSales-Peres, ArsenioRibeiro-Bicudo, Lucilene Arilhoda Silva, Ricardo Henrique Alvesinfo:eu-repo/semantics/openAccess2013-11-11T12:48:37Zoai:ojs.revistaseletronicas.pucrs.br:article/5043Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2013-11-11T12:48:37Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.none.fl_str_mv |
Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry Avaliação da qualidade do DNA extraído de saliva humana armazenada e sua aplicabilidade na identificação em Odontologia Legal |
title |
Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry |
spellingShingle |
Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry Carvalho, Suzana Papile Maciel Human identification Forensic Dentistry Forensic Medicine DNA molecular biology saliva Identificação Humana |
title_short |
Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry |
title_full |
Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry |
title_fullStr |
Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry |
title_full_unstemmed |
Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry |
title_sort |
Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry |
author |
Carvalho, Suzana Papile Maciel |
author_facet |
Carvalho, Suzana Papile Maciel Sales-Peres, Arsenio Ribeiro-Bicudo, Lucilene Arilho da Silva, Ricardo Henrique Alves |
author_role |
author |
author2 |
Sales-Peres, Arsenio Ribeiro-Bicudo, Lucilene Arilho da Silva, Ricardo Henrique Alves |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Carvalho, Suzana Papile Maciel Sales-Peres, Arsenio Ribeiro-Bicudo, Lucilene Arilho da Silva, Ricardo Henrique Alves |
dc.subject.por.fl_str_mv |
Human identification Forensic Dentistry Forensic Medicine DNA molecular biology saliva Identificação Humana |
topic |
Human identification Forensic Dentistry Forensic Medicine DNA molecular biology saliva Identificação Humana |
description |
Purpose: This study evaluated the quality of DNA obtained from stored human saliva and its applicability to human identification. Methods: The saliva samples of 20 subjects, collected in the form of saliva in natura and from mouth swabs and stored at -20ºC, were analyzed. After 7 days, the DNA was extracted from the 40 saliva samples and subjected to PCR and electrophoresis. After 180 days, the technique was repeated with the 20 swab samples. Results: The first-stage results indicated that DNA was successfully extracted in 97.5% of reactions, 95% of saliva in natura and 100% of swab saliva samples, with no statistically significant difference between the forms of saliva. In the second phase, the result was positive for all 20 analyzed samples (100%). Subsequently, in order to analyze the quality of the DNA obtained from human saliva, the SIX3-2 gene was tested on the 20 mouth swab samples, and the PCR products were digested using the MbO1 restriction enzyme to evaluate polymorphisms in the ADRA-2 gene, with positive results for most samples. Conclusion: It was concluded that the quantity and quality of DNA from saliva and the techniques employed are adequate for forensic analysis of DNA. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5043 |
url |
https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5043 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5043/4861 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
EDIPUCRS - Editora Universitária da PUCRS |
publisher.none.fl_str_mv |
EDIPUCRS - Editora Universitária da PUCRS |
dc.source.none.fl_str_mv |
Revista Odonto Ciência; Vol. 25 No. 1 (2010); 48 - 53 Revista Odonto Ciência; v. 25 n. 1 (2010); 48 - 53 1980-6523 0102-9460 reponame:Revista odonto ciência (Online) instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) instacron:PUC_RS |
instname_str |
Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
instacron_str |
PUC_RS |
institution |
PUC_RS |
reponame_str |
Revista odonto ciência (Online) |
collection |
Revista odonto ciência (Online) |
repository.name.fl_str_mv |
Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
repository.mail.fl_str_mv |
||odontociencia@pucrs.br |
_version_ |
1754820874885660672 |