Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry

Detalhes bibliográficos
Autor(a) principal: Carvalho, Suzana Papile Maciel
Data de Publicação: 2010
Outros Autores: Sales-Peres, Arsenio, Ribeiro-Bicudo, Lucilene Arilho, da Silva, Ricardo Henrique Alves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista odonto ciência (Online)
Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5043
Resumo: Purpose: This study evaluated the quality of DNA obtained from stored human saliva and its applicability to human identification. Methods: The saliva samples of 20 subjects, collected in the form of saliva in natura and from mouth swabs and stored at -20ºC, were analyzed. After 7 days, the DNA was extracted from the 40 saliva samples and subjected to PCR and electrophoresis. After 180 days, the technique was repeated with the 20 swab samples. Results: The first-stage results indicated that DNA was successfully extracted in 97.5% of reactions, 95% of saliva in natura and 100% of swab saliva samples, with no statistically significant difference between the forms of saliva. In the second phase, the result was positive for all 20 analyzed samples (100%). Subsequently, in order to analyze the quality of the DNA obtained from human saliva, the SIX3-2 gene was tested on the 20 mouth swab samples, and the PCR products were digested using the MbO1 restriction enzyme to evaluate polymorphisms in the ADRA-2 gene, with positive results for most samples. Conclusion: It was concluded that the quantity and quality of DNA from saliva and the techniques employed are adequate for forensic analysis of DNA.
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spelling Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic DentistryAvaliação da qualidade do DNA extraído de saliva humana armazenada e sua aplicabilidade na identificação em Odontologia LegalHuman identificationForensic DentistryForensic MedicineDNAmolecular biologysalivaIdentificação HumanaPurpose: This study evaluated the quality of DNA obtained from stored human saliva and its applicability to human identification. Methods: The saliva samples of 20 subjects, collected in the form of saliva in natura and from mouth swabs and stored at -20ºC, were analyzed. After 7 days, the DNA was extracted from the 40 saliva samples and subjected to PCR and electrophoresis. After 180 days, the technique was repeated with the 20 swab samples. Results: The first-stage results indicated that DNA was successfully extracted in 97.5% of reactions, 95% of saliva in natura and 100% of swab saliva samples, with no statistically significant difference between the forms of saliva. In the second phase, the result was positive for all 20 analyzed samples (100%). Subsequently, in order to analyze the quality of the DNA obtained from human saliva, the SIX3-2 gene was tested on the 20 mouth swab samples, and the PCR products were digested using the MbO1 restriction enzyme to evaluate polymorphisms in the ADRA-2 gene, with positive results for most samples. Conclusion: It was concluded that the quantity and quality of DNA from saliva and the techniques employed are adequate for forensic analysis of DNA.Este trabalho objetivou avaliar a qualidade do DNA obtido de saliva humana armazenada e sua aplicabilidade da identificação de pessoas. Metodologia: Analisaram-se amostras salivares de n=20 sujeitos da pesquisa, coletadas nas formas de saliva in natura e de swab bucal, sendo armazenadas a 20ºC. Após 7 dias, o DNA foi extraído das 40 amostras de saliva e submetido à PCR e à eletroforese. Após 180 dias repetiu-se a técnica nas 20 amostras de swab. Resultados: Os resultados da primeira etapa indicaram que o DNA foi extraído com sucesso em 97,5% das reações, e, analisando-se separadamente, em 95% de saliva in natura e em 100% da saliva do swab, não havendo diferenças estatisticamente significantes entre as duas formas de saliva. Na segunda fase, o resultado foi positivo para as 20 amostras analisadas (100%). Posteriormente, para analisar a qualidade do DNA obtido da saliva humana, o gene SIX3-2 foi testado nas 20 amostras de swab bucal e foi feita a digestão do produto da PCR com a enzima de restrição MbO1 para avaliar polimorfismo do gene ADRA-2 obtendo-se resultados positivos para a maioria das amostras. Conclusão: Concluiu-se que a quantidade e a qualidade do DNA advindo de saliva e as técnicas empregadas estão adequadas à análise forense do DNA.EDIPUCRS - Editora Universitária da PUCRS2010-01-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articleapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5043Revista Odonto Ciência; Vol. 25 No. 1 (2010); 48 - 53Revista Odonto Ciência; v. 25 n. 1 (2010); 48 - 531980-65230102-9460reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5043/4861Carvalho, Suzana Papile MacielSales-Peres, ArsenioRibeiro-Bicudo, Lucilene Arilhoda Silva, Ricardo Henrique Alvesinfo:eu-repo/semantics/openAccess2013-11-11T12:48:37Zoai:ojs.revistaseletronicas.pucrs.br:article/5043Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2013-11-11T12:48:37Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry
Avaliação da qualidade do DNA extraído de saliva humana armazenada e sua aplicabilidade na identificação em Odontologia Legal
title Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry
spellingShingle Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry
Carvalho, Suzana Papile Maciel
Human identification
Forensic Dentistry
Forensic Medicine
DNA
molecular biology
saliva
Identificação Humana
title_short Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry
title_full Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry
title_fullStr Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry
title_full_unstemmed Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry
title_sort Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry
author Carvalho, Suzana Papile Maciel
author_facet Carvalho, Suzana Papile Maciel
Sales-Peres, Arsenio
Ribeiro-Bicudo, Lucilene Arilho
da Silva, Ricardo Henrique Alves
author_role author
author2 Sales-Peres, Arsenio
Ribeiro-Bicudo, Lucilene Arilho
da Silva, Ricardo Henrique Alves
author2_role author
author
author
dc.contributor.author.fl_str_mv Carvalho, Suzana Papile Maciel
Sales-Peres, Arsenio
Ribeiro-Bicudo, Lucilene Arilho
da Silva, Ricardo Henrique Alves
dc.subject.por.fl_str_mv Human identification
Forensic Dentistry
Forensic Medicine
DNA
molecular biology
saliva
Identificação Humana
topic Human identification
Forensic Dentistry
Forensic Medicine
DNA
molecular biology
saliva
Identificação Humana
description Purpose: This study evaluated the quality of DNA obtained from stored human saliva and its applicability to human identification. Methods: The saliva samples of 20 subjects, collected in the form of saliva in natura and from mouth swabs and stored at -20ºC, were analyzed. After 7 days, the DNA was extracted from the 40 saliva samples and subjected to PCR and electrophoresis. After 180 days, the technique was repeated with the 20 swab samples. Results: The first-stage results indicated that DNA was successfully extracted in 97.5% of reactions, 95% of saliva in natura and 100% of swab saliva samples, with no statistically significant difference between the forms of saliva. In the second phase, the result was positive for all 20 analyzed samples (100%). Subsequently, in order to analyze the quality of the DNA obtained from human saliva, the SIX3-2 gene was tested on the 20 mouth swab samples, and the PCR products were digested using the MbO1 restriction enzyme to evaluate polymorphisms in the ADRA-2 gene, with positive results for most samples. Conclusion: It was concluded that the quantity and quality of DNA from saliva and the techniques employed are adequate for forensic analysis of DNA.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5043
url https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5043
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5043/4861
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
dc.source.none.fl_str_mv Revista Odonto Ciência; Vol. 25 No. 1 (2010); 48 - 53
Revista Odonto Ciência; v. 25 n. 1 (2010); 48 - 53
1980-6523
0102-9460
reponame:Revista odonto ciência (Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Revista odonto ciência (Online)
collection Revista odonto ciência (Online)
repository.name.fl_str_mv Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv ||odontociencia@pucrs.br
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