Immunoexpression of PCNA and α-SMA in gingival overgrowth tissues

• Autor(a) principal: Fonseca, Luciara Leão Viana
• Data de Publicação: 2016
• Outros Autores: Andrade, Ana Luiza Dias Leite de, Galvão, Hébel Cavalcanti, Rosa, Marize Raquel Diniz da, Bonan, Paulo Rogério Ferreti
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista odonto ciência (Online)
• Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/15303
• topic: Gingival overgrowth; Phenytoin; Nifedipine; Gingival fibromatosis; Crescimento gengival; Fenitoína; Nifedipina; Fibromatose gengival

Purpose: This study aimed to investigate proliferating cell nuclear antigen (PCNA) and isoform-α of the smooth muscle actin (α-SMA) immunoexpression, as well as the epithelial thickness in druginduced gingival overgrowth (DIGO) and idiopathic gingival fibromatosis (IGF) patients.Methods: Gingival samples were obtained from 11 users of nifedipine and phenytoin, 6 of patients with IGF and 4 fibrous inflammatory hyperplasia. The specimens were submitted to immunohistochemical and morphological analysis.Results: Our results show that PCNA-epithelial positive cells were slightly more common among IGF patients, but there were not statistically significant differences among the groups (p>0.05). Comparing the vessels counts with α SMA-positive pericyte or smooth muscular cells, there was not statistical differences, although the control group presented a discrete higher count (p>0.05). The epithelial thickness analysis revealed that the DIGO group presented the higher mean, evidencing statistically significant differences in relation to the control group (p=0,002).Conclusion: In conclusion, our results demonstrated similarities between DIGO and IGF according to PCNA and α-SMA immunoexpression, although the epithelial thickness was higher in DIGO group.