Microbiological evaluation of peracetic acid for disinfection of acrylic resins

Detalhes bibliográficos
Autor(a) principal: Stopiglia, Cheila Denise Ottonelli
Data de Publicação: 2011
Outros Autores: Carissimi, Mariana, Scroferneker, Maria Lúcia, Fortes, Carmen Beatriz Borges
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista odonto ciência (Online)
Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/8735
Resumo: Purpose: The aim of this study was to assess the efficacy of peracetic acid (PAA) for the disinfection of dental acrylic resins experimentally contaminated with Candida albicans, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Methods: Fifteen materials were used for each type of resin (thermosetting, self-curing and microwave-curing). Each material was placed in a test tube containing culture medium with a suspension of each microorganism and then incubated. The materials were rinsed and transferred to other tubes containing 50 mL of water for 5 min, 0.2% peracetic acid for 5 min or glutaraldehyde for 30 min. The materials were placed in the culture agar and incubated. Microbial growth was determined by colony counting after plating. Results: Candida albicans growth was inhibited by peracetic acid and glutaraldehyde treatments. The number of colonies on resins treated with saline was greater than 105 CFU/mL. In resins infected with E. coli, S. aureus and P. aeruginosa the colony growth was not inhibited by saline and peracetic acid, but it was totally inhibited by glutaraldehyde. Conclusion: Surface disinfection using peracetic acid effectively inhibited C. albicans growth on all acrylic resins.
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spelling Microbiological evaluation of peracetic acid for disinfection of acrylic resinsAvaliação microbiológica de ácido peracético na desinfecção de resinas acrílicasPeracetic acidacrylic resinsCandida albicansÁcido peracéticoresinas acrílicasCandida albicansPurpose: The aim of this study was to assess the efficacy of peracetic acid (PAA) for the disinfection of dental acrylic resins experimentally contaminated with Candida albicans, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Methods: Fifteen materials were used for each type of resin (thermosetting, self-curing and microwave-curing). Each material was placed in a test tube containing culture medium with a suspension of each microorganism and then incubated. The materials were rinsed and transferred to other tubes containing 50 mL of water for 5 min, 0.2% peracetic acid for 5 min or glutaraldehyde for 30 min. The materials were placed in the culture agar and incubated. Microbial growth was determined by colony counting after plating. Results: Candida albicans growth was inhibited by peracetic acid and glutaraldehyde treatments. The number of colonies on resins treated with saline was greater than 105 CFU/mL. In resins infected with E. coli, S. aureus and P. aeruginosa the colony growth was not inhibited by saline and peracetic acid, but it was totally inhibited by glutaraldehyde. Conclusion: Surface disinfection using peracetic acid effectively inhibited C. albicans growth on all acrylic resins.Objetivo: O objetivo deste estudo foi avaliar a eficácia do ácido peracético (PAA) na desinfecção de resinas acrílicas dentais experimentalmente contaminadas com Candida albicans, Escherichia coli, Staphylococcus aureus e Pseudomonas aeruginosa. Metodologia: Quinze corpos de prova (CP) foram utilizados para cada tipo de resina (termopolimerizáveis, autopolimerizáveis e ativados por energia de micro-ondas). Cada CP foi colocado em um tubo teste com meio de cultura contendo uma suspensão de cada microrganismo e incubado. Posteriormente, os CP foram lavados e transferidos para outros tubos contendo 50 mL de água por 5 minutos, em 0,2% de ácido peracético por 5 min ou em glutaraldeído por 30 minutos, plaqueados em ágar de cultura e incubados. O crescimento microbiano foi determinado por contagem de colônias após o plaqueamento. Resultado: O crescimento de Candida albicans foi inibido nos tratamentos com ácido peracético e glutaraldeído. O número de colônias nas resinas tratadas com solução salina foi superior a 105 UFC/mL. Nas resinas infectadas com E. coli, S. aureus e P. aeruginosa, o crescimento das colônias não foi inibido nas resinas tratadas com salina e ácido peracético, mas foi totalmente inibida pelo glutaraldeído. Conclusão: A desinfecção com ácido peracético inibiu efetivamente o crescimento de C. albicans em todas as resinas acrílicas.EDIPUCRS - Editora Universitária da PUCRS2011-08-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articleapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/8735Revista Odonto Ciência; Vol. 26 No. 3 (2011); 238 - 241Revista Odonto Ciência; v. 26 n. 3 (2011); 238 - 2411980-65230102-9460reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/8735/6885Stopiglia, Cheila Denise OttonelliCarissimi, MarianaScroferneker, Maria LúciaFortes, Carmen Beatriz Borgesinfo:eu-repo/semantics/openAccess2013-11-11T11:52:28Zoai:ojs.revistaseletronicas.pucrs.br:article/8735Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2013-11-11T11:52:28Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Microbiological evaluation of peracetic acid for disinfection of acrylic resins
Avaliação microbiológica de ácido peracético na desinfecção de resinas acrílicas
title Microbiological evaluation of peracetic acid for disinfection of acrylic resins
spellingShingle Microbiological evaluation of peracetic acid for disinfection of acrylic resins
Stopiglia, Cheila Denise Ottonelli
Peracetic acid
acrylic resins
Candida albicans
Ácido peracético
resinas acrílicas
Candida albicans
title_short Microbiological evaluation of peracetic acid for disinfection of acrylic resins
title_full Microbiological evaluation of peracetic acid for disinfection of acrylic resins
title_fullStr Microbiological evaluation of peracetic acid for disinfection of acrylic resins
title_full_unstemmed Microbiological evaluation of peracetic acid for disinfection of acrylic resins
title_sort Microbiological evaluation of peracetic acid for disinfection of acrylic resins
author Stopiglia, Cheila Denise Ottonelli
author_facet Stopiglia, Cheila Denise Ottonelli
Carissimi, Mariana
Scroferneker, Maria Lúcia
Fortes, Carmen Beatriz Borges
author_role author
author2 Carissimi, Mariana
Scroferneker, Maria Lúcia
Fortes, Carmen Beatriz Borges
author2_role author
author
author
dc.contributor.author.fl_str_mv Stopiglia, Cheila Denise Ottonelli
Carissimi, Mariana
Scroferneker, Maria Lúcia
Fortes, Carmen Beatriz Borges
dc.subject.por.fl_str_mv Peracetic acid
acrylic resins
Candida albicans
Ácido peracético
resinas acrílicas
Candida albicans
topic Peracetic acid
acrylic resins
Candida albicans
Ácido peracético
resinas acrílicas
Candida albicans
description Purpose: The aim of this study was to assess the efficacy of peracetic acid (PAA) for the disinfection of dental acrylic resins experimentally contaminated with Candida albicans, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Methods: Fifteen materials were used for each type of resin (thermosetting, self-curing and microwave-curing). Each material was placed in a test tube containing culture medium with a suspension of each microorganism and then incubated. The materials were rinsed and transferred to other tubes containing 50 mL of water for 5 min, 0.2% peracetic acid for 5 min or glutaraldehyde for 30 min. The materials were placed in the culture agar and incubated. Microbial growth was determined by colony counting after plating. Results: Candida albicans growth was inhibited by peracetic acid and glutaraldehyde treatments. The number of colonies on resins treated with saline was greater than 105 CFU/mL. In resins infected with E. coli, S. aureus and P. aeruginosa the colony growth was not inhibited by saline and peracetic acid, but it was totally inhibited by glutaraldehyde. Conclusion: Surface disinfection using peracetic acid effectively inhibited C. albicans growth on all acrylic resins.
publishDate 2011
dc.date.none.fl_str_mv 2011-08-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/8735
url https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/8735
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/8735/6885
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
dc.source.none.fl_str_mv Revista Odonto Ciência; Vol. 26 No. 3 (2011); 238 - 241
Revista Odonto Ciência; v. 26 n. 3 (2011); 238 - 241
1980-6523
0102-9460
reponame:Revista odonto ciência (Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Revista odonto ciência (Online)
collection Revista odonto ciência (Online)
repository.name.fl_str_mv Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv ||odontociencia@pucrs.br
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