Immunohistochemical expression of pericytes and myofibroblasts in the extracellular matrix of oral actinic cheilitis and squamous cell carcinoma: a comparative study

Detalhes bibliográficos
Autor(a) principal: Wanderley, Flávia Godinho
Data de Publicação: 2017
Outros Autores: Nunes, Carlla Silva, Ferreira, Maira Sá, Gonzalez, Ana Cristina, Reis, Silvia Regina Almeida, Medrado, Alena Ribeiro Alves Peixoto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista odonto ciência (Online)
Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/23344
Resumo: OBJECTIVE: The aim of the present study is to conduct a comparative analysis of oral actinic cheilitis and squamous cell carcinomas by immunohistochemical analysis.METHODS: This cross-sectional study utilizes a convenience sample obtained from the archives of an oral pathology laboratory from a higher education institution. Tissue sections from patients diagnosed with actinic cheilitis and carcinoma were immunohistochemically analyzed using monoclonal antibodies specific for smooth muscle alpha actin (SMA) and neuron-glial antigen 2 (NG2). Individual cells that stained positive for these proteins were counted in specific fields by means of histomorphometry.RESULTS: Subsequent statistical analysis revealed a higher number of NG2-positive when compared with SMA-positive cells in both lesion types (p = 0.005) and these cells were located adjacent to blood vessels. The differences between normal oral mucosa, severe dysplasia, and carcinoma specimens were statistically significant (p = 0.009; p = 0.006).CONCLUSION: These data strongly suggest that increased expression of NG2- and SMA-positive cells is associated with formation of blood capillaries, highlighting the importance of angiogenesis in tumor progression.
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spelling Immunohistochemical expression of pericytes and myofibroblasts in the extracellular matrix of oral actinic cheilitis and squamous cell carcinoma: a comparative studyExpressão imuno-histoquímica de pericitos e miofibroblastos na matriz extracelular de queilite actínica oral e carcinoma de células escamosas: estudo comparativoCarcinomaPericytesMyofibroblastsOral PathologyCarcinomaPericitosMiofibroblastosOBJECTIVE: The aim of the present study is to conduct a comparative analysis of oral actinic cheilitis and squamous cell carcinomas by immunohistochemical analysis.METHODS: This cross-sectional study utilizes a convenience sample obtained from the archives of an oral pathology laboratory from a higher education institution. Tissue sections from patients diagnosed with actinic cheilitis and carcinoma were immunohistochemically analyzed using monoclonal antibodies specific for smooth muscle alpha actin (SMA) and neuron-glial antigen 2 (NG2). Individual cells that stained positive for these proteins were counted in specific fields by means of histomorphometry.RESULTS: Subsequent statistical analysis revealed a higher number of NG2-positive when compared with SMA-positive cells in both lesion types (p = 0.005) and these cells were located adjacent to blood vessels. The differences between normal oral mucosa, severe dysplasia, and carcinoma specimens were statistically significant (p = 0.009; p = 0.006).CONCLUSION: These data strongly suggest that increased expression of NG2- and SMA-positive cells is associated with formation of blood capillaries, highlighting the importance of angiogenesis in tumor progression.OBJETIVO: O objetivo do presente estudo foi realizar uma análise comparativa da queilite actínica e do carcinoma de células escamosas por meio de análise imuno-histoquímica.MÉTODOS: Este estudo transversal utilizou uma amostra de conveniência obtida dos arquivos de um laboratório de patologia oral de uma instituição de ensino superior. As secções de tecido de pacientes diagnosticados com queiliteactínica e carcinoma foram analisados pela técnica imuno-histoquímica usando anticorpos monoclonais específicos para alfa actina de músculo liso (SMA) e do antígeno neurônio/glial2 (ng2). As células com marcação positiva para estas proteínas foram contadas em campos específicos por meio de histomorfometria.RESULTADOS: análise estatística subsequente revelou maior número de positivos para ng2 quando comparados com células SMA positivas em ambos os tipos de lesão (p = 0,005) seno que estas células foram localizadas adjacentes aos vasos sanguíneos. As diferenças entre mucosa oral normal, displasia grave e espécimes de carcinoma foram estatisticamente significativas (p = 0,009; p = 0,006).CONCLUSÃO: Os dados sugerem, fortemente, que o aumento da expressão de células positivas para ng2 e SMA está associado à formação de capilares sanguíneos, destacando a importância da angiogênese na progressão tumoral.EDIPUCRS - Editora Universitária da PUCRS2017-10-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articleapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2334410.15448/1980-6523.2017.1.23344Revista Odonto Ciência; Vol. 32 No. 1 (2017); 28-34Revista Odonto Ciência; v. 32 n. 1 (2017); 28-341980-65230102-946010.15448/1980-6523.2017.1reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/23344/15975Copyright (c) 2017 Revista Odonto Ciênciainfo:eu-repo/semantics/openAccessWanderley, Flávia GodinhoNunes, Carlla SilvaFerreira, Maira SáGonzalez, Ana CristinaReis, Silvia Regina AlmeidaMedrado, Alena Ribeiro Alves Peixoto2018-04-04T16:33:35Zoai:ojs.revistaseletronicas.pucrs.br:article/23344Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2018-04-04T16:33:35Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Immunohistochemical expression of pericytes and myofibroblasts in the extracellular matrix of oral actinic cheilitis and squamous cell carcinoma: a comparative study
Expressão imuno-histoquímica de pericitos e miofibroblastos na matriz extracelular de queilite actínica oral e carcinoma de células escamosas: estudo comparativo
title Immunohistochemical expression of pericytes and myofibroblasts in the extracellular matrix of oral actinic cheilitis and squamous cell carcinoma: a comparative study
spellingShingle Immunohistochemical expression of pericytes and myofibroblasts in the extracellular matrix of oral actinic cheilitis and squamous cell carcinoma: a comparative study
Wanderley, Flávia Godinho
Carcinoma
Pericytes
Myofibroblasts
Oral Pathology
Carcinoma
Pericitos
Miofibroblastos
title_short Immunohistochemical expression of pericytes and myofibroblasts in the extracellular matrix of oral actinic cheilitis and squamous cell carcinoma: a comparative study
title_full Immunohistochemical expression of pericytes and myofibroblasts in the extracellular matrix of oral actinic cheilitis and squamous cell carcinoma: a comparative study
title_fullStr Immunohistochemical expression of pericytes and myofibroblasts in the extracellular matrix of oral actinic cheilitis and squamous cell carcinoma: a comparative study
title_full_unstemmed Immunohistochemical expression of pericytes and myofibroblasts in the extracellular matrix of oral actinic cheilitis and squamous cell carcinoma: a comparative study
title_sort Immunohistochemical expression of pericytes and myofibroblasts in the extracellular matrix of oral actinic cheilitis and squamous cell carcinoma: a comparative study
author Wanderley, Flávia Godinho
author_facet Wanderley, Flávia Godinho
Nunes, Carlla Silva
Ferreira, Maira Sá
Gonzalez, Ana Cristina
Reis, Silvia Regina Almeida
Medrado, Alena Ribeiro Alves Peixoto
author_role author
author2 Nunes, Carlla Silva
Ferreira, Maira Sá
Gonzalez, Ana Cristina
Reis, Silvia Regina Almeida
Medrado, Alena Ribeiro Alves Peixoto
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Wanderley, Flávia Godinho
Nunes, Carlla Silva
Ferreira, Maira Sá
Gonzalez, Ana Cristina
Reis, Silvia Regina Almeida
Medrado, Alena Ribeiro Alves Peixoto
dc.subject.por.fl_str_mv Carcinoma
Pericytes
Myofibroblasts
Oral Pathology
Carcinoma
Pericitos
Miofibroblastos
topic Carcinoma
Pericytes
Myofibroblasts
Oral Pathology
Carcinoma
Pericitos
Miofibroblastos
description OBJECTIVE: The aim of the present study is to conduct a comparative analysis of oral actinic cheilitis and squamous cell carcinomas by immunohistochemical analysis.METHODS: This cross-sectional study utilizes a convenience sample obtained from the archives of an oral pathology laboratory from a higher education institution. Tissue sections from patients diagnosed with actinic cheilitis and carcinoma were immunohistochemically analyzed using monoclonal antibodies specific for smooth muscle alpha actin (SMA) and neuron-glial antigen 2 (NG2). Individual cells that stained positive for these proteins were counted in specific fields by means of histomorphometry.RESULTS: Subsequent statistical analysis revealed a higher number of NG2-positive when compared with SMA-positive cells in both lesion types (p = 0.005) and these cells were located adjacent to blood vessels. The differences between normal oral mucosa, severe dysplasia, and carcinoma specimens were statistically significant (p = 0.009; p = 0.006).CONCLUSION: These data strongly suggest that increased expression of NG2- and SMA-positive cells is associated with formation of blood capillaries, highlighting the importance of angiogenesis in tumor progression.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-03
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/23344
10.15448/1980-6523.2017.1.23344
url https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/23344
identifier_str_mv 10.15448/1980-6523.2017.1.23344
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/23344/15975
dc.rights.driver.fl_str_mv Copyright (c) 2017 Revista Odonto Ciência
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Revista Odonto Ciência
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
dc.source.none.fl_str_mv Revista Odonto Ciência; Vol. 32 No. 1 (2017); 28-34
Revista Odonto Ciência; v. 32 n. 1 (2017); 28-34
1980-6523
0102-9460
10.15448/1980-6523.2017.1
reponame:Revista odonto ciência (Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Revista odonto ciência (Online)
collection Revista odonto ciência (Online)
repository.name.fl_str_mv Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv ||odontociencia@pucrs.br
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