In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa

Detalhes bibliográficos
Autor(a) principal: Semenoff, Tereza Aparecida Delle Vedove
Data de Publicação: 2008
Outros Autores: Semenoff Segundo, Alex, Biasoli, Éder Ricardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista odonto ciência (Online)
Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2293
Resumo: Purpose: The purpose of this study was to compare the in vitro antimicrobial effectiveness of Periogard®, Cepacol®, and Plax® against Staphylococcus aureus and Pseudomonas aeruginosa. Methods: Sixteen Petri dishes with blood agar and 16 with Mueller-Hinton agar culture media were used. Four dishes of each culture media were used as negative and positive controls. In the remaining 24 dishes, 4 wet discs were distributed in each Petri dish, as follows: 1 soaked in distilled water (control), 1 in Periogard®, 1 in Plax®, and 1 in Cepacol®. After 48h the inhibition halos of microbial growth were measured with digital calipers. Data were analyzed by ANOVA and Bonferroni test (α=0.05). Results: Regarding P. Aeruginosa, Periogard® was significantly more effective than the other mouthwashes (P< 0.05), followed by Cepacol; Plax® and distilled water did not show inhibition of microorganism growth. Regarding S. aureus, all mouthwashes had significant inhibitory effect in the following descending order: Plax® > Periogard® > Cepacol® (P< 0.05). Conclusion: The results suggest that the tested mouthwashes show different inhibitory potential for growth of Staphylococcus aureus and Pseudomonas aeruginosa. Key words: Mouthwashes; Staphylococcus aureus; Pseudomonas aeruginosa; chlorhexidine; triclosan; cetylpyridinium
id PUC_RS-22_9cf1953ded5641598f114bbdc3029413
oai_identifier_str oai:ojs.revistaseletronicas.pucrs.br:article/2293
network_acronym_str PUC_RS-22
network_name_str Revista odonto ciência (Online)
repository_id_str
spelling In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosaEfetividade antimicrobiana in vitro de enxaguatórios bucais frente aos microorganismos Staphylococcus aureus e Pseudomonas aeruginosaMouthwashesStaphylococcus aureusPseudomonas aeruginosachlorhexidinetriclosancetylpyridiniumPeriodonticsMicrobiologyPurpose: The purpose of this study was to compare the in vitro antimicrobial effectiveness of Periogard®, Cepacol®, and Plax® against Staphylococcus aureus and Pseudomonas aeruginosa. Methods: Sixteen Petri dishes with blood agar and 16 with Mueller-Hinton agar culture media were used. Four dishes of each culture media were used as negative and positive controls. In the remaining 24 dishes, 4 wet discs were distributed in each Petri dish, as follows: 1 soaked in distilled water (control), 1 in Periogard®, 1 in Plax®, and 1 in Cepacol®. After 48h the inhibition halos of microbial growth were measured with digital calipers. Data were analyzed by ANOVA and Bonferroni test (α=0.05). Results: Regarding P. Aeruginosa, Periogard® was significantly more effective than the other mouthwashes (P< 0.05), followed by Cepacol; Plax® and distilled water did not show inhibition of microorganism growth. Regarding S. aureus, all mouthwashes had significant inhibitory effect in the following descending order: Plax® > Periogard® > Cepacol® (P< 0.05). Conclusion: The results suggest that the tested mouthwashes show different inhibitory potential for growth of Staphylococcus aureus and Pseudomonas aeruginosa. Key words: Mouthwashes; Staphylococcus aureus; Pseudomonas aeruginosa; chlorhexidine; triclosan; cetylpyridiniumObjetivo: Este estudo comparou a efetividade antimicrobiana in vitro dos enxaguatórios bucais Periogard®, Cepacol® e Plax® nos microrganismos Staphylococcus aureus e Pseudomonas aeruginosa. Metodologia: Utilizaram-se 16 placas de Petri, com meio de cultura Ágar Sangue e 16 com Mueller Hinton. Quatro placas de cada meio foram usadas como controle positivo e negativo. Nas 24 placas restantes, distribuíram-se 4 discos umedecidos em cada placa, sendo: 1 em água destilada (controle), 1 em Periogard®, 1 em Plax® e 1 em Cepacol®. Após 48h, os halos de inibição de crescimento dos microrganismos foram medidos com paquímetro digital. Os dados foram analisados por ANOVA e teste de Bonferroni (α=0,05). Resultados: Em relação a P. aeruginosa, o Periogard® foi a substância mais efetiva (P< 0,05), seguida pelo Cepacol®; Plax® e água destilada não tiveram efeito inibitório. Para S. aureus, todas as substâncias apresentaram efetividade antimicrobiana, em ordem decrescente: Plax® > Periogard® > Cepacol® (P< 0,05). Conclusão: Os resultados sugerem que os enxaguatórios bucais testados apresentam diferente potencial de inibição para o crescimento de Staphylococcus aureus e Pseudomonas aeruginosa. Palavras-chave: Anti-sépticos bucais; Staphylococcus aureus; Pseudomonas aeruginosa; Clorexidina; Triclosan; CetilpiridínioEDIPUCRS - Editora Universitária da PUCRS2008-06-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articlecell cultureapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2293Revista Odonto Ciência; Vol. 23 No. 4 (2008); 351 - 354Revista Odonto Ciência; v. 23 n. 4 (2008); 351 - 3541980-65230102-9460reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2293/3434Semenoff, Tereza Aparecida Delle VedoveSemenoff Segundo, AlexBiasoli, Éder Ricardoinfo:eu-repo/semantics/openAccess2013-11-18T10:32:04Zoai:ojs.revistaseletronicas.pucrs.br:article/2293Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2013-11-18T10:32:04Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa
Efetividade antimicrobiana in vitro de enxaguatórios bucais frente aos microorganismos Staphylococcus aureus e Pseudomonas aeruginosa
title In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa
spellingShingle In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa
Semenoff, Tereza Aparecida Delle Vedove
Mouthwashes
Staphylococcus aureus
Pseudomonas aeruginosa
chlorhexidine
triclosan
cetylpyridinium
Periodontics
Microbiology
title_short In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa
title_full In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa
title_fullStr In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa
title_full_unstemmed In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa
title_sort In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa
author Semenoff, Tereza Aparecida Delle Vedove
author_facet Semenoff, Tereza Aparecida Delle Vedove
Semenoff Segundo, Alex
Biasoli, Éder Ricardo
author_role author
author2 Semenoff Segundo, Alex
Biasoli, Éder Ricardo
author2_role author
author
dc.contributor.author.fl_str_mv Semenoff, Tereza Aparecida Delle Vedove
Semenoff Segundo, Alex
Biasoli, Éder Ricardo
dc.subject.por.fl_str_mv Mouthwashes
Staphylococcus aureus
Pseudomonas aeruginosa
chlorhexidine
triclosan
cetylpyridinium
Periodontics
Microbiology
topic Mouthwashes
Staphylococcus aureus
Pseudomonas aeruginosa
chlorhexidine
triclosan
cetylpyridinium
Periodontics
Microbiology
description Purpose: The purpose of this study was to compare the in vitro antimicrobial effectiveness of Periogard®, Cepacol®, and Plax® against Staphylococcus aureus and Pseudomonas aeruginosa. Methods: Sixteen Petri dishes with blood agar and 16 with Mueller-Hinton agar culture media were used. Four dishes of each culture media were used as negative and positive controls. In the remaining 24 dishes, 4 wet discs were distributed in each Petri dish, as follows: 1 soaked in distilled water (control), 1 in Periogard®, 1 in Plax®, and 1 in Cepacol®. After 48h the inhibition halos of microbial growth were measured with digital calipers. Data were analyzed by ANOVA and Bonferroni test (α=0.05). Results: Regarding P. Aeruginosa, Periogard® was significantly more effective than the other mouthwashes (P< 0.05), followed by Cepacol; Plax® and distilled water did not show inhibition of microorganism growth. Regarding S. aureus, all mouthwashes had significant inhibitory effect in the following descending order: Plax® > Periogard® > Cepacol® (P< 0.05). Conclusion: The results suggest that the tested mouthwashes show different inhibitory potential for growth of Staphylococcus aureus and Pseudomonas aeruginosa. Key words: Mouthwashes; Staphylococcus aureus; Pseudomonas aeruginosa; chlorhexidine; triclosan; cetylpyridinium
publishDate 2008
dc.date.none.fl_str_mv 2008-06-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
cell culture
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2293
url https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2293
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2293/3434
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
dc.source.none.fl_str_mv Revista Odonto Ciência; Vol. 23 No. 4 (2008); 351 - 354
Revista Odonto Ciência; v. 23 n. 4 (2008); 351 - 354
1980-6523
0102-9460
reponame:Revista odonto ciência (Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Revista odonto ciência (Online)
collection Revista odonto ciência (Online)
repository.name.fl_str_mv Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv ||odontociencia@pucrs.br
_version_ 1754820873801433088