In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista odonto ciência (Online) |
Texto Completo: | https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2293 |
Resumo: | Purpose: The purpose of this study was to compare the in vitro antimicrobial effectiveness of Periogard®, Cepacol®, and Plax® against Staphylococcus aureus and Pseudomonas aeruginosa. Methods: Sixteen Petri dishes with blood agar and 16 with Mueller-Hinton agar culture media were used. Four dishes of each culture media were used as negative and positive controls. In the remaining 24 dishes, 4 wet discs were distributed in each Petri dish, as follows: 1 soaked in distilled water (control), 1 in Periogard®, 1 in Plax®, and 1 in Cepacol®. After 48h the inhibition halos of microbial growth were measured with digital calipers. Data were analyzed by ANOVA and Bonferroni test (α=0.05). Results: Regarding P. Aeruginosa, Periogard® was significantly more effective than the other mouthwashes (P< 0.05), followed by Cepacol; Plax® and distilled water did not show inhibition of microorganism growth. Regarding S. aureus, all mouthwashes had significant inhibitory effect in the following descending order: Plax® > Periogard® > Cepacol® (P< 0.05). Conclusion: The results suggest that the tested mouthwashes show different inhibitory potential for growth of Staphylococcus aureus and Pseudomonas aeruginosa. Key words: Mouthwashes; Staphylococcus aureus; Pseudomonas aeruginosa; chlorhexidine; triclosan; cetylpyridinium |
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In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosaEfetividade antimicrobiana in vitro de enxaguatórios bucais frente aos microorganismos Staphylococcus aureus e Pseudomonas aeruginosaMouthwashesStaphylococcus aureusPseudomonas aeruginosachlorhexidinetriclosancetylpyridiniumPeriodonticsMicrobiologyPurpose: The purpose of this study was to compare the in vitro antimicrobial effectiveness of Periogard®, Cepacol®, and Plax® against Staphylococcus aureus and Pseudomonas aeruginosa. Methods: Sixteen Petri dishes with blood agar and 16 with Mueller-Hinton agar culture media were used. Four dishes of each culture media were used as negative and positive controls. In the remaining 24 dishes, 4 wet discs were distributed in each Petri dish, as follows: 1 soaked in distilled water (control), 1 in Periogard®, 1 in Plax®, and 1 in Cepacol®. After 48h the inhibition halos of microbial growth were measured with digital calipers. Data were analyzed by ANOVA and Bonferroni test (α=0.05). Results: Regarding P. Aeruginosa, Periogard® was significantly more effective than the other mouthwashes (P< 0.05), followed by Cepacol; Plax® and distilled water did not show inhibition of microorganism growth. Regarding S. aureus, all mouthwashes had significant inhibitory effect in the following descending order: Plax® > Periogard® > Cepacol® (P< 0.05). Conclusion: The results suggest that the tested mouthwashes show different inhibitory potential for growth of Staphylococcus aureus and Pseudomonas aeruginosa. Key words: Mouthwashes; Staphylococcus aureus; Pseudomonas aeruginosa; chlorhexidine; triclosan; cetylpyridiniumObjetivo: Este estudo comparou a efetividade antimicrobiana in vitro dos enxaguatórios bucais Periogard®, Cepacol® e Plax® nos microrganismos Staphylococcus aureus e Pseudomonas aeruginosa. Metodologia: Utilizaram-se 16 placas de Petri, com meio de cultura Ágar Sangue e 16 com Mueller Hinton. Quatro placas de cada meio foram usadas como controle positivo e negativo. Nas 24 placas restantes, distribuíram-se 4 discos umedecidos em cada placa, sendo: 1 em água destilada (controle), 1 em Periogard®, 1 em Plax® e 1 em Cepacol®. Após 48h, os halos de inibição de crescimento dos microrganismos foram medidos com paquímetro digital. Os dados foram analisados por ANOVA e teste de Bonferroni (α=0,05). Resultados: Em relação a P. aeruginosa, o Periogard® foi a substância mais efetiva (P< 0,05), seguida pelo Cepacol®; Plax® e água destilada não tiveram efeito inibitório. Para S. aureus, todas as substâncias apresentaram efetividade antimicrobiana, em ordem decrescente: Plax® > Periogard® > Cepacol® (P< 0,05). Conclusão: Os resultados sugerem que os enxaguatórios bucais testados apresentam diferente potencial de inibição para o crescimento de Staphylococcus aureus e Pseudomonas aeruginosa. Palavras-chave: Anti-sépticos bucais; Staphylococcus aureus; Pseudomonas aeruginosa; Clorexidina; Triclosan; CetilpiridínioEDIPUCRS - Editora Universitária da PUCRS2008-06-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articlecell cultureapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2293Revista Odonto Ciência; Vol. 23 No. 4 (2008); 351 - 354Revista Odonto Ciência; v. 23 n. 4 (2008); 351 - 3541980-65230102-9460reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2293/3434Semenoff, Tereza Aparecida Delle VedoveSemenoff Segundo, AlexBiasoli, Éder Ricardoinfo:eu-repo/semantics/openAccess2013-11-18T10:32:04Zoai:ojs.revistaseletronicas.pucrs.br:article/2293Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2013-11-18T10:32:04Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.none.fl_str_mv |
In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa Efetividade antimicrobiana in vitro de enxaguatórios bucais frente aos microorganismos Staphylococcus aureus e Pseudomonas aeruginosa |
title |
In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa |
spellingShingle |
In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa Semenoff, Tereza Aparecida Delle Vedove Mouthwashes Staphylococcus aureus Pseudomonas aeruginosa chlorhexidine triclosan cetylpyridinium Periodontics Microbiology |
title_short |
In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa |
title_full |
In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa |
title_fullStr |
In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa |
title_full_unstemmed |
In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa |
title_sort |
In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa |
author |
Semenoff, Tereza Aparecida Delle Vedove |
author_facet |
Semenoff, Tereza Aparecida Delle Vedove Semenoff Segundo, Alex Biasoli, Éder Ricardo |
author_role |
author |
author2 |
Semenoff Segundo, Alex Biasoli, Éder Ricardo |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Semenoff, Tereza Aparecida Delle Vedove Semenoff Segundo, Alex Biasoli, Éder Ricardo |
dc.subject.por.fl_str_mv |
Mouthwashes Staphylococcus aureus Pseudomonas aeruginosa chlorhexidine triclosan cetylpyridinium Periodontics Microbiology |
topic |
Mouthwashes Staphylococcus aureus Pseudomonas aeruginosa chlorhexidine triclosan cetylpyridinium Periodontics Microbiology |
description |
Purpose: The purpose of this study was to compare the in vitro antimicrobial effectiveness of Periogard®, Cepacol®, and Plax® against Staphylococcus aureus and Pseudomonas aeruginosa. Methods: Sixteen Petri dishes with blood agar and 16 with Mueller-Hinton agar culture media were used. Four dishes of each culture media were used as negative and positive controls. In the remaining 24 dishes, 4 wet discs were distributed in each Petri dish, as follows: 1 soaked in distilled water (control), 1 in Periogard®, 1 in Plax®, and 1 in Cepacol®. After 48h the inhibition halos of microbial growth were measured with digital calipers. Data were analyzed by ANOVA and Bonferroni test (α=0.05). Results: Regarding P. Aeruginosa, Periogard® was significantly more effective than the other mouthwashes (P< 0.05), followed by Cepacol; Plax® and distilled water did not show inhibition of microorganism growth. Regarding S. aureus, all mouthwashes had significant inhibitory effect in the following descending order: Plax® > Periogard® > Cepacol® (P< 0.05). Conclusion: The results suggest that the tested mouthwashes show different inhibitory potential for growth of Staphylococcus aureus and Pseudomonas aeruginosa. Key words: Mouthwashes; Staphylococcus aureus; Pseudomonas aeruginosa; chlorhexidine; triclosan; cetylpyridinium |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-06-22 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article cell culture |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2293 |
url |
https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2293 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2293/3434 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
EDIPUCRS - Editora Universitária da PUCRS |
publisher.none.fl_str_mv |
EDIPUCRS - Editora Universitária da PUCRS |
dc.source.none.fl_str_mv |
Revista Odonto Ciência; Vol. 23 No. 4 (2008); 351 - 354 Revista Odonto Ciência; v. 23 n. 4 (2008); 351 - 354 1980-6523 0102-9460 reponame:Revista odonto ciência (Online) instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) instacron:PUC_RS |
instname_str |
Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
instacron_str |
PUC_RS |
institution |
PUC_RS |
reponame_str |
Revista odonto ciência (Online) |
collection |
Revista odonto ciência (Online) |
repository.name.fl_str_mv |
Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
repository.mail.fl_str_mv |
||odontociencia@pucrs.br |
_version_ |
1754820873801433088 |