Antimicrobial action of an intracanal medication trial using Aloe vera
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista odonto ciência (Online) |
Texto Completo: | https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/15717 |
Resumo: | Objective: This study aimed to test the calcium hydroxide associated with vehicles Aloe vera, chlorhexidine digluconate (2%) and saline, in inhibiting bacterial growth on Mueller-Hinton agar plates.Methods: The species Staphiloccocus aureus, Streptococcus pyogenes, Escherichia coli and Enterecoccus faecalis were isolated and inoculated in 3 mL of BHI (Brain Heart Infusion). Holes (5 mm diameter) were made in the plates and filled with the test materials. After incubation, readings were taken with a hand lens and a caliper with 0.1 mm accuracy to determine the diameter of the inhibition zone, after 24 and 48 hours. Each experiment was repeated six times, and the average values were obtained.Results: Chlorhexidine digluconate without calcium hydroxide resulted in better inhibition of bacterial growth, followed by the hydroxide pastes evaluated. E. coli strains were the most resistant to the tested compounds, followed by S. pyogenes and S. aureus. There was no statistically significant interaction between the variables.Conclusion: Chlorhexidine digluconate (2%) alone showed the best antimicrobial effectiveness. Aloe vera is a promising vehicle for the calcium hydroxide but more studies should be conducted on herbal medicines in dentistry. |
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Antimicrobial action of an intracanal medication trial using Aloe veraAção antimicrobiana de uma medicação intracanal experimental utilizando-se Aloe veraMicrobiologyEndodonticsPhytotherapyPhytotherapeutic drugsAloe veraMicrobiologiaEndodontiaFitoterapiaMedicamentos fitoterápicosAloe veraObjective: This study aimed to test the calcium hydroxide associated with vehicles Aloe vera, chlorhexidine digluconate (2%) and saline, in inhibiting bacterial growth on Mueller-Hinton agar plates.Methods: The species Staphiloccocus aureus, Streptococcus pyogenes, Escherichia coli and Enterecoccus faecalis were isolated and inoculated in 3 mL of BHI (Brain Heart Infusion). Holes (5 mm diameter) were made in the plates and filled with the test materials. After incubation, readings were taken with a hand lens and a caliper with 0.1 mm accuracy to determine the diameter of the inhibition zone, after 24 and 48 hours. Each experiment was repeated six times, and the average values were obtained.Results: Chlorhexidine digluconate without calcium hydroxide resulted in better inhibition of bacterial growth, followed by the hydroxide pastes evaluated. E. coli strains were the most resistant to the tested compounds, followed by S. pyogenes and S. aureus. There was no statistically significant interaction between the variables.Conclusion: Chlorhexidine digluconate (2%) alone showed the best antimicrobial effectiveness. Aloe vera is a promising vehicle for the calcium hydroxide but more studies should be conducted on herbal medicines in dentistry.Objetivo: Este estudo teve como objetivo testar o hidróxido de cálcio associado com os veículos Aloe vera, digluconato de clorexidina (2%) e solução salina, na inibição do crescimento bacteriano em placas de ágar Mueller-Hinton.Métodos: As espécies Staphiloccocus aureus, Streptococcus pyogenes, Escherichia coli e Enterecoccus faecalis foram isoladas e inoculadas em 3 mL de BHI (Brain Heart Infusion). Orifícios de 5 mm de diâmetro foram feitos nas placas e as substâncias a serem testadas foram inseridas. Após a incubação, as leituras dos halos de inibição foram aferidas com lente de mão e cursor com precisão de 0,1 mm a fim de determinar o diâmetro da zona de inibição, após 24 e 48 horas. Cada experimento foi repetido seis vezes, e os valores médios foram obtidos.Resultados: Digluconato de clorexidina (2%) isolado apresentou melhor inibição do crescimento bacteriano, seguido pelas pastas de hidróxido de cálcio avaliadas. Bactérias E. coli foram as mais resistentes aos compostos testados, seguidas por S. pyogenes e S. aureus. Não houve interação significativa entre as variáveis.Conclusão: Digluconato de clorexidina (2%) isolado apresentou melhor efetividade antimicrobiana. Aloe vera é um veículo promissor para o hidróxido de cálcio, entretanto mais estudos devem ser realizados com medicamentos fitoterápicos na Odontologia.EDIPUCRS - Editora Universitária da PUCRS2016-01-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articleapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/1571710.15448/1980-6523.2015.4.15717Revista Odonto Ciência; Vol. 30 No. 4 (2015); 153-156Revista Odonto Ciência; v. 30 n. 4 (2015); 153-1561980-65230102-946010.15448/1980-6523.2015.4reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/15717/14448Copyright (c) 2016 Revista Odonto Ciênciainfo:eu-repo/semantics/openAccessBeserra, Amanda Pereirae Silva, Débora LimaFé, Jessyca Leal MouraSoares, Isadora Mello VilarinhoParanagua, Josie Haydée Lima FerreiraCarvalho, Carmen Milena Rodrigues Siqueira2016-06-16T13:45:46Zoai:ojs.revistaseletronicas.pucrs.br:article/15717Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2016-06-16T13:45:46Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.none.fl_str_mv |
Antimicrobial action of an intracanal medication trial using Aloe vera Ação antimicrobiana de uma medicação intracanal experimental utilizando-se Aloe vera |
title |
Antimicrobial action of an intracanal medication trial using Aloe vera |
spellingShingle |
Antimicrobial action of an intracanal medication trial using Aloe vera Beserra, Amanda Pereira Microbiology Endodontics Phytotherapy Phytotherapeutic drugs Aloe vera Microbiologia Endodontia Fitoterapia Medicamentos fitoterápicos Aloe vera |
title_short |
Antimicrobial action of an intracanal medication trial using Aloe vera |
title_full |
Antimicrobial action of an intracanal medication trial using Aloe vera |
title_fullStr |
Antimicrobial action of an intracanal medication trial using Aloe vera |
title_full_unstemmed |
Antimicrobial action of an intracanal medication trial using Aloe vera |
title_sort |
Antimicrobial action of an intracanal medication trial using Aloe vera |
author |
Beserra, Amanda Pereira |
author_facet |
Beserra, Amanda Pereira e Silva, Débora Lima Fé, Jessyca Leal Moura Soares, Isadora Mello Vilarinho Paranagua, Josie Haydée Lima Ferreira Carvalho, Carmen Milena Rodrigues Siqueira |
author_role |
author |
author2 |
e Silva, Débora Lima Fé, Jessyca Leal Moura Soares, Isadora Mello Vilarinho Paranagua, Josie Haydée Lima Ferreira Carvalho, Carmen Milena Rodrigues Siqueira |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Beserra, Amanda Pereira e Silva, Débora Lima Fé, Jessyca Leal Moura Soares, Isadora Mello Vilarinho Paranagua, Josie Haydée Lima Ferreira Carvalho, Carmen Milena Rodrigues Siqueira |
dc.subject.por.fl_str_mv |
Microbiology Endodontics Phytotherapy Phytotherapeutic drugs Aloe vera Microbiologia Endodontia Fitoterapia Medicamentos fitoterápicos Aloe vera |
topic |
Microbiology Endodontics Phytotherapy Phytotherapeutic drugs Aloe vera Microbiologia Endodontia Fitoterapia Medicamentos fitoterápicos Aloe vera |
description |
Objective: This study aimed to test the calcium hydroxide associated with vehicles Aloe vera, chlorhexidine digluconate (2%) and saline, in inhibiting bacterial growth on Mueller-Hinton agar plates.Methods: The species Staphiloccocus aureus, Streptococcus pyogenes, Escherichia coli and Enterecoccus faecalis were isolated and inoculated in 3 mL of BHI (Brain Heart Infusion). Holes (5 mm diameter) were made in the plates and filled with the test materials. After incubation, readings were taken with a hand lens and a caliper with 0.1 mm accuracy to determine the diameter of the inhibition zone, after 24 and 48 hours. Each experiment was repeated six times, and the average values were obtained.Results: Chlorhexidine digluconate without calcium hydroxide resulted in better inhibition of bacterial growth, followed by the hydroxide pastes evaluated. E. coli strains were the most resistant to the tested compounds, followed by S. pyogenes and S. aureus. There was no statistically significant interaction between the variables.Conclusion: Chlorhexidine digluconate (2%) alone showed the best antimicrobial effectiveness. Aloe vera is a promising vehicle for the calcium hydroxide but more studies should be conducted on herbal medicines in dentistry. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/15717 10.15448/1980-6523.2015.4.15717 |
url |
https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/15717 |
identifier_str_mv |
10.15448/1980-6523.2015.4.15717 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/15717/14448 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2016 Revista Odonto Ciência info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2016 Revista Odonto Ciência |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
EDIPUCRS - Editora Universitária da PUCRS |
publisher.none.fl_str_mv |
EDIPUCRS - Editora Universitária da PUCRS |
dc.source.none.fl_str_mv |
Revista Odonto Ciência; Vol. 30 No. 4 (2015); 153-156 Revista Odonto Ciência; v. 30 n. 4 (2015); 153-156 1980-6523 0102-9460 10.15448/1980-6523.2015.4 reponame:Revista odonto ciência (Online) instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) instacron:PUC_RS |
instname_str |
Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
instacron_str |
PUC_RS |
institution |
PUC_RS |
reponame_str |
Revista odonto ciência (Online) |
collection |
Revista odonto ciência (Online) |
repository.name.fl_str_mv |
Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
repository.mail.fl_str_mv |
||odontociencia@pucrs.br |
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1754820876543459328 |