Antimicrobial action of an intracanal medication trial using Aloe vera

Detalhes bibliográficos
Autor(a) principal: Beserra, Amanda Pereira
Data de Publicação: 2016
Outros Autores: e Silva, Débora Lima, Fé, Jessyca Leal Moura, Soares, Isadora Mello Vilarinho, Paranagua, Josie Haydée Lima Ferreira, Carvalho, Carmen Milena Rodrigues Siqueira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista odonto ciência (Online)
Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/15717
Resumo: Objective: This study aimed to test the calcium hydroxide associated with vehicles Aloe vera, chlorhexidine digluconate (2%) and saline, in inhibiting bacterial growth on Mueller-Hinton agar plates.Methods: The species Staphiloccocus aureus, Streptococcus pyogenes, Escherichia coli and Enterecoccus faecalis were isolated and inoculated in 3 mL of BHI (Brain Heart Infusion). Holes (5 mm diameter) were made in the plates and filled with the test materials. After incubation, readings were taken with a hand lens and a caliper with 0.1 mm accuracy to determine the diameter of the inhibition zone, after 24 and 48 hours. Each experiment was repeated six times, and the average values were obtained.Results: Chlorhexidine digluconate without calcium hydroxide resulted in better inhibition of bacterial growth, followed by the hydroxide pastes evaluated. E. coli strains were the most resistant to the tested compounds, followed by S. pyogenes and S. aureus. There was no statistically significant interaction between the variables.Conclusion: Chlorhexidine digluconate (2%) alone showed the best antimicrobial effectiveness. Aloe vera is a promising vehicle for the calcium hydroxide but more studies should be conducted on herbal medicines in dentistry.
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spelling Antimicrobial action of an intracanal medication trial using Aloe veraAção antimicrobiana de uma medicação intracanal experimental utilizando-se Aloe veraMicrobiologyEndodonticsPhytotherapyPhytotherapeutic drugsAloe veraMicrobiologiaEndodontiaFitoterapiaMedicamentos fitoterápicosAloe veraObjective: This study aimed to test the calcium hydroxide associated with vehicles Aloe vera, chlorhexidine digluconate (2%) and saline, in inhibiting bacterial growth on Mueller-Hinton agar plates.Methods: The species Staphiloccocus aureus, Streptococcus pyogenes, Escherichia coli and Enterecoccus faecalis were isolated and inoculated in 3 mL of BHI (Brain Heart Infusion). Holes (5 mm diameter) were made in the plates and filled with the test materials. After incubation, readings were taken with a hand lens and a caliper with 0.1 mm accuracy to determine the diameter of the inhibition zone, after 24 and 48 hours. Each experiment was repeated six times, and the average values were obtained.Results: Chlorhexidine digluconate without calcium hydroxide resulted in better inhibition of bacterial growth, followed by the hydroxide pastes evaluated. E. coli strains were the most resistant to the tested compounds, followed by S. pyogenes and S. aureus. There was no statistically significant interaction between the variables.Conclusion: Chlorhexidine digluconate (2%) alone showed the best antimicrobial effectiveness. Aloe vera is a promising vehicle for the calcium hydroxide but more studies should be conducted on herbal medicines in dentistry.Objetivo: Este estudo teve como objetivo testar o hidróxido de cálcio associado com os veículos Aloe vera, digluconato de clorexidina (2%) e solução salina, na inibição do crescimento bacteriano em placas de ágar Mueller-Hinton.Métodos: As espécies Staphiloccocus aureus, Streptococcus pyogenes, Escherichia coli e Enterecoccus faecalis foram isoladas e inoculadas em 3 mL de BHI (Brain Heart Infusion). Orifícios de 5 mm de diâmetro foram feitos nas placas e as substâncias a serem testadas foram inseridas. Após a incubação, as leituras dos halos de inibição foram aferidas com lente de mão e cursor com precisão de 0,1 mm a fim de determinar o diâmetro da zona de inibição, após 24 e 48 horas. Cada experimento foi repetido seis vezes, e os valores médios foram obtidos.Resultados: Digluconato de clorexidina (2%) isolado apresentou melhor inibição do crescimento bacteriano, seguido pelas pastas de hidróxido de cálcio avaliadas. Bactérias E. coli foram as mais resistentes aos compostos testados, seguidas por S. pyogenes e S. aureus. Não houve interação significativa entre as variáveis.Conclusão: Digluconato de clorexidina (2%) isolado apresentou melhor efetividade antimicrobiana. Aloe vera é um veículo promissor para o hidróxido de cálcio, entretanto mais estudos devem ser realizados com medicamentos fitoterápicos na Odontologia.EDIPUCRS - Editora Universitária da PUCRS2016-01-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articleapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/1571710.15448/1980-6523.2015.4.15717Revista Odonto Ciência; Vol. 30 No. 4 (2015); 153-156Revista Odonto Ciência; v. 30 n. 4 (2015); 153-1561980-65230102-946010.15448/1980-6523.2015.4reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/15717/14448Copyright (c) 2016 Revista Odonto Ciênciainfo:eu-repo/semantics/openAccessBeserra, Amanda Pereirae Silva, Débora LimaFé, Jessyca Leal MouraSoares, Isadora Mello VilarinhoParanagua, Josie Haydée Lima FerreiraCarvalho, Carmen Milena Rodrigues Siqueira2016-06-16T13:45:46Zoai:ojs.revistaseletronicas.pucrs.br:article/15717Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2016-06-16T13:45:46Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Antimicrobial action of an intracanal medication trial using Aloe vera
Ação antimicrobiana de uma medicação intracanal experimental utilizando-se Aloe vera
title Antimicrobial action of an intracanal medication trial using Aloe vera
spellingShingle Antimicrobial action of an intracanal medication trial using Aloe vera
Beserra, Amanda Pereira
Microbiology
Endodontics
Phytotherapy
Phytotherapeutic drugs
Aloe vera
Microbiologia
Endodontia
Fitoterapia
Medicamentos fitoterápicos
Aloe vera
title_short Antimicrobial action of an intracanal medication trial using Aloe vera
title_full Antimicrobial action of an intracanal medication trial using Aloe vera
title_fullStr Antimicrobial action of an intracanal medication trial using Aloe vera
title_full_unstemmed Antimicrobial action of an intracanal medication trial using Aloe vera
title_sort Antimicrobial action of an intracanal medication trial using Aloe vera
author Beserra, Amanda Pereira
author_facet Beserra, Amanda Pereira
e Silva, Débora Lima
Fé, Jessyca Leal Moura
Soares, Isadora Mello Vilarinho
Paranagua, Josie Haydée Lima Ferreira
Carvalho, Carmen Milena Rodrigues Siqueira
author_role author
author2 e Silva, Débora Lima
Fé, Jessyca Leal Moura
Soares, Isadora Mello Vilarinho
Paranagua, Josie Haydée Lima Ferreira
Carvalho, Carmen Milena Rodrigues Siqueira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Beserra, Amanda Pereira
e Silva, Débora Lima
Fé, Jessyca Leal Moura
Soares, Isadora Mello Vilarinho
Paranagua, Josie Haydée Lima Ferreira
Carvalho, Carmen Milena Rodrigues Siqueira
dc.subject.por.fl_str_mv Microbiology
Endodontics
Phytotherapy
Phytotherapeutic drugs
Aloe vera
Microbiologia
Endodontia
Fitoterapia
Medicamentos fitoterápicos
Aloe vera
topic Microbiology
Endodontics
Phytotherapy
Phytotherapeutic drugs
Aloe vera
Microbiologia
Endodontia
Fitoterapia
Medicamentos fitoterápicos
Aloe vera
description Objective: This study aimed to test the calcium hydroxide associated with vehicles Aloe vera, chlorhexidine digluconate (2%) and saline, in inhibiting bacterial growth on Mueller-Hinton agar plates.Methods: The species Staphiloccocus aureus, Streptococcus pyogenes, Escherichia coli and Enterecoccus faecalis were isolated and inoculated in 3 mL of BHI (Brain Heart Infusion). Holes (5 mm diameter) were made in the plates and filled with the test materials. After incubation, readings were taken with a hand lens and a caliper with 0.1 mm accuracy to determine the diameter of the inhibition zone, after 24 and 48 hours. Each experiment was repeated six times, and the average values were obtained.Results: Chlorhexidine digluconate without calcium hydroxide resulted in better inhibition of bacterial growth, followed by the hydroxide pastes evaluated. E. coli strains were the most resistant to the tested compounds, followed by S. pyogenes and S. aureus. There was no statistically significant interaction between the variables.Conclusion: Chlorhexidine digluconate (2%) alone showed the best antimicrobial effectiveness. Aloe vera is a promising vehicle for the calcium hydroxide but more studies should be conducted on herbal medicines in dentistry.
publishDate 2016
dc.date.none.fl_str_mv 2016-01-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/15717
10.15448/1980-6523.2015.4.15717
url https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/15717
identifier_str_mv 10.15448/1980-6523.2015.4.15717
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/15717/14448
dc.rights.driver.fl_str_mv Copyright (c) 2016 Revista Odonto Ciência
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2016 Revista Odonto Ciência
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
dc.source.none.fl_str_mv Revista Odonto Ciência; Vol. 30 No. 4 (2015); 153-156
Revista Odonto Ciência; v. 30 n. 4 (2015); 153-156
1980-6523
0102-9460
10.15448/1980-6523.2015.4
reponame:Revista odonto ciência (Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Revista odonto ciência (Online)
collection Revista odonto ciência (Online)
repository.name.fl_str_mv Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv ||odontociencia@pucrs.br
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