Evaluation of the aggressiveness of peripheral giant cell lesion of the oral cavity by means of a hystochemical-immunohystochemical study

Detalhes bibliográficos
Autor(a) principal: De Carli, João Paulo
Data de Publicação: 2008
Outros Autores: Moraes, Norberto Perri, Crivelini, Marcelo Macedo, da Silva, Soluete Oliveira, Linden, Maria Salete Sandini, Trentin, Micheline Sandini
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista odonto ciência (Online)
Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2651
Resumo: Purpose: To study the aggressiveness of peripheral giant cell lesion (PGCL) using hystochemical-immunohystochemical analysis. Methods: The archives of the Dental School of Araçatuba (FOA-UNESP) and the University of Passo Fundo Dental School (FOUPF) were searched. A total of 181 PGCL cases were retrieved, and 61 met the study eligibility criteria. Firstly, the PGCL aggressiveness was graded, and the lesions were distributed into three groups based on clinical and radiographic findings. The 61 LCGPs hystopathological samples were colored by H.E. and analyzed for diagnostic confirmation. Afterwards, the cellular proliferation of 15 PGCLs cases was investigated by means of counting and calibrating of AgNORs (nucleolar organizer regions) area and evaluation of the immunoexpression of PCNA, Ki-67, and p53 markers. Results: No difference was found for AgNOR area and PCNA expression among PGCLs of the three studied groups, and p53 was not an effective immunomarker of the analyzed lesions. There was a statistically significant difference of AgNORs number and Ki-67 expression among the three groups. Conclusion: The AgNOR and Ki-67 results suggest that the PGCL clinical-radiographic aggressiveness can be related to its cellular proliferative activity. Key words: Giant cell peripheric lesion; diagnosis; silver staining; immunohystochemistry
id PUC_RS-22_b86f9f81984e13d409cb5673f72cd295
oai_identifier_str oai:ojs.revistaseletronicas.pucrs.br:article/2651
network_acronym_str PUC_RS-22
network_name_str Revista odonto ciência (Online)
repository_id_str
spelling Evaluation of the aggressiveness of peripheral giant cell lesion of the oral cavity by means of a hystochemical-immunohystochemical studyAvaliação da agressividade da lesão de células gigantes periférica da cavidade bucal através de estudo histoquímico-imunohistoquímicoGiant cell peripheric lesiondiagnosissilver stainingimmunohystochemistryGiant cell peripheric lesionPurpose: To study the aggressiveness of peripheral giant cell lesion (PGCL) using hystochemical-immunohystochemical analysis. Methods: The archives of the Dental School of Araçatuba (FOA-UNESP) and the University of Passo Fundo Dental School (FOUPF) were searched. A total of 181 PGCL cases were retrieved, and 61 met the study eligibility criteria. Firstly, the PGCL aggressiveness was graded, and the lesions were distributed into three groups based on clinical and radiographic findings. The 61 LCGPs hystopathological samples were colored by H.E. and analyzed for diagnostic confirmation. Afterwards, the cellular proliferation of 15 PGCLs cases was investigated by means of counting and calibrating of AgNORs (nucleolar organizer regions) area and evaluation of the immunoexpression of PCNA, Ki-67, and p53 markers. Results: No difference was found for AgNOR area and PCNA expression among PGCLs of the three studied groups, and p53 was not an effective immunomarker of the analyzed lesions. There was a statistically significant difference of AgNORs number and Ki-67 expression among the three groups. Conclusion: The AgNOR and Ki-67 results suggest that the PGCL clinical-radiographic aggressiveness can be related to its cellular proliferative activity. Key words: Giant cell peripheric lesion; diagnosis; silver staining; immunohystochemistryObjetivo: Estudar a agressividade da lesão de células gigantes periférica (LCGP) através de um estudo histoquímico e imunohistoquímico. Metodologia: Foram revisados os arquivos da Faculdade de Odontologia de Araçatuba (FOA-UNESP) e da Faculdade de Odontologia da Universidade de Passo Fundo (FOUPF), tendo sido obtidos 181 casos de LCGP, dos quais 61 preencheram os critérios de elegibilidade. Primeiramente, graduou-se a agressividade das LCGPs, que foram distribuídas em três grupos de estudo com base em achados clínicos e radiográficos. Em seguida, os cortes histológicos das 61 LCGPs, corados por H.E., foram analisados para confirmação diagnóstica. Posteriormente, investigou-se a proliferação celular de 15 casos de LCGPs através de contagem e aferição da área de AgNORs (regiões de organização nucleolar) e avaliação da imunoexpressão dos marcadores PCNA, Ki-67 e p53. Resultados: Não se constatou diferença na área das AgNORs e na expressão do PCNA entre as LCGPs dos três grupos estudados, e o p53 não foi efetivo na imunomarcação das lesões analisadas. Notou-se diferença estatisticamente significativa no número de AgNORs e na expressão do Ki-67 entre os grupos estudados. Conclusão: Os resultados de AgNOR e Ki-67 sugerem que a agressividade clínicoradiográfica das LCGPs pode ser relacionada com sua atividade proliferativa celular. Palavras-chave: Lesão periférica de células gigantes; diagnóstico; impregnação pela prata; imunohistoquímicaEDIPUCRS - Editora Universitária da PUCRS2008-06-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articleobservational; descriptiveapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2651Revista Odonto Ciência; Vol. 23 No. 3 (2008); 291 - 296Revista Odonto Ciência; v. 23 n. 3 (2008); 291 - 2961980-65230102-9460reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2651/3194De Carli, João PauloMoraes, Norberto PerriCrivelini, Marcelo Macedoda Silva, Soluete OliveiraLinden, Maria Salete SandiniTrentin, Micheline Sandiniinfo:eu-repo/semantics/openAccess2013-11-18T10:39:58Zoai:ojs.revistaseletronicas.pucrs.br:article/2651Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2013-11-18T10:39:58Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Evaluation of the aggressiveness of peripheral giant cell lesion of the oral cavity by means of a hystochemical-immunohystochemical study
Avaliação da agressividade da lesão de células gigantes periférica da cavidade bucal através de estudo histoquímico-imunohistoquímico
title Evaluation of the aggressiveness of peripheral giant cell lesion of the oral cavity by means of a hystochemical-immunohystochemical study
spellingShingle Evaluation of the aggressiveness of peripheral giant cell lesion of the oral cavity by means of a hystochemical-immunohystochemical study
De Carli, João Paulo
Giant cell peripheric lesion
diagnosis
silver staining
immunohystochemistry
Giant cell peripheric lesion
title_short Evaluation of the aggressiveness of peripheral giant cell lesion of the oral cavity by means of a hystochemical-immunohystochemical study
title_full Evaluation of the aggressiveness of peripheral giant cell lesion of the oral cavity by means of a hystochemical-immunohystochemical study
title_fullStr Evaluation of the aggressiveness of peripheral giant cell lesion of the oral cavity by means of a hystochemical-immunohystochemical study
title_full_unstemmed Evaluation of the aggressiveness of peripheral giant cell lesion of the oral cavity by means of a hystochemical-immunohystochemical study
title_sort Evaluation of the aggressiveness of peripheral giant cell lesion of the oral cavity by means of a hystochemical-immunohystochemical study
author De Carli, João Paulo
author_facet De Carli, João Paulo
Moraes, Norberto Perri
Crivelini, Marcelo Macedo
da Silva, Soluete Oliveira
Linden, Maria Salete Sandini
Trentin, Micheline Sandini
author_role author
author2 Moraes, Norberto Perri
Crivelini, Marcelo Macedo
da Silva, Soluete Oliveira
Linden, Maria Salete Sandini
Trentin, Micheline Sandini
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv De Carli, João Paulo
Moraes, Norberto Perri
Crivelini, Marcelo Macedo
da Silva, Soluete Oliveira
Linden, Maria Salete Sandini
Trentin, Micheline Sandini
dc.subject.por.fl_str_mv Giant cell peripheric lesion
diagnosis
silver staining
immunohystochemistry
Giant cell peripheric lesion
topic Giant cell peripheric lesion
diagnosis
silver staining
immunohystochemistry
Giant cell peripheric lesion
description Purpose: To study the aggressiveness of peripheral giant cell lesion (PGCL) using hystochemical-immunohystochemical analysis. Methods: The archives of the Dental School of Araçatuba (FOA-UNESP) and the University of Passo Fundo Dental School (FOUPF) were searched. A total of 181 PGCL cases were retrieved, and 61 met the study eligibility criteria. Firstly, the PGCL aggressiveness was graded, and the lesions were distributed into three groups based on clinical and radiographic findings. The 61 LCGPs hystopathological samples were colored by H.E. and analyzed for diagnostic confirmation. Afterwards, the cellular proliferation of 15 PGCLs cases was investigated by means of counting and calibrating of AgNORs (nucleolar organizer regions) area and evaluation of the immunoexpression of PCNA, Ki-67, and p53 markers. Results: No difference was found for AgNOR area and PCNA expression among PGCLs of the three studied groups, and p53 was not an effective immunomarker of the analyzed lesions. There was a statistically significant difference of AgNORs number and Ki-67 expression among the three groups. Conclusion: The AgNOR and Ki-67 results suggest that the PGCL clinical-radiographic aggressiveness can be related to its cellular proliferative activity. Key words: Giant cell peripheric lesion; diagnosis; silver staining; immunohystochemistry
publishDate 2008
dc.date.none.fl_str_mv 2008-06-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
observational; descriptive
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2651
url https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2651
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2651/3194
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
dc.source.none.fl_str_mv Revista Odonto Ciência; Vol. 23 No. 3 (2008); 291 - 296
Revista Odonto Ciência; v. 23 n. 3 (2008); 291 - 296
1980-6523
0102-9460
reponame:Revista odonto ciência (Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Revista odonto ciência (Online)
collection Revista odonto ciência (Online)
repository.name.fl_str_mv Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv ||odontociencia@pucrs.br
_version_ 1754820874237640704

Registros relacionados